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题名福建省属公益类科研院所运行状况及成效分析
被引量：1
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作者
许正春
池敏青
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机构
福建省农业科学院农业经济与科技信息研究所
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出处
《福建农业科技》
2018年第12期38-41,共4页
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基金
福建省科技计划项目--省属公益类科研院所基本科研专项(2016R1015-3)
福建省农业科学院"十三五"青年科技创新团队(STIT2017-3-7)
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文摘
在新一轮公益类科研院所分类改革背景下,总结近10年来福建省属公益类科研院所的发展概况及政府对该类院所的投入情况。以2015年数据为例,综述福建省属公益类科研院所从科技人才建设、科技经费投入、科技平台与固定资产建设等方面科技资源投入和配置情况,从获奖、知识产权、行业标准、论文论著、科技成果转化及对外科技服务等方面分析分类改革后福建省属公益类科研院所取得成效。
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关键词
省属公益类科研院所
分类改革
运行状况
成效
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Keywords
Provincial public welfare research institutions
restructuring of different categories of institutions
running situation
achievements
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分类号
G322.7
[文化科学]
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题名中韩野生软枣猕猴桃种质资源遗传多样性分析
被引量：13
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作者
赵成日
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机构
延边大学农学院
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出处
《果树学报》
CAS
CSCD
北大核心
2018年第9期1043-1051,共9页
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基金
国家自然科学基金(31660319)
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文摘
【目的】利用RAPD分子标记技术研究国内长白山地区和韩国由来野生软枣猕猴桃种质资源的遗传多样性。【方法】以长白山地区和韩国由来的28个野生软枣猕猴桃的叶片为材料,利用RAPD分子标记技术进行了遗传多样性分析,并明确了它们之间的亲缘关系。【结果】利用131个随机引物进行PCR,从中筛选出了多态性高、重复性好且扩增条带清晰的24个引物。24个引物在28个野生软枣猕猴桃中共扩增出191条带,其中多态性条带为186条,占97.4%。平均每个引物产生7.75个多态性条带。应用NTSYSpc 2.10e软件进行遗传一致度和遗传距离分析后用UPGMA方法进行聚类分析。28个野生软枣猕猴桃种质资源之间的遗传距离为0.020 2~0.934 2。遗传距离0.58时可将28个野生种划分成2大类。在遗传距离最小的0.02处,有蛟河2号和3号2个野生种,其RAPD分子标记相似性为98%。【结论】来自不同地理区域的野生软枣猕猴桃之间存在较高的遗传多样性,而在同一地理区域内遗传多样性较低。韩国野生软枣猕猴桃之间的遗传多样性较低,且与二道白河、汪清、左家等地的野生软枣猕猴桃亲缘关系较近。即具有同一地理区域聚类趋势,且不同地理区域间存在较高的遗传多样性。
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关键词
软枣猕猴桃
遗传多样性
RAPD
长白山地区
韩国
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Keywords
Actinidia arguta
Gendenaturation at 94 ℃ for 1 min
annealing at 36 ℃for 1 min
extension at 72 ℃ for 2 min
reaction of 40 cycles
last extension at 72 ℃ for 5 min. The same template was repeated twice. RAPD-PCR amplification products were analyzed by 0.8% agarose gel （0.5 ×TAE buffer） electrophoresis. [Results]The 24 primers had high genetic polymorphisms. A total of 191 bands were amplified
of which 186 were polymorphic bands
accounting for 97.4%. Average 7.75 polymorphic bands were produced by each primer. Genetic identity and genetic distance were calculated by NTSYSpc 2.10e software and cluster analysis was analyzed by UPGMA. The result showed that the genetic distances among the 28 wild species ofA. arguta germplasm resources were 0.020 2-0.934 2. When the genetic distance was 0.58
the 28 wild species could be divided into two groups. The first group included Antu coloring type
Antu No.1-6 fromAntu County and Jiaohe No. 1-5 from Jiaohe. The second group include： ErdaobaiheNo. 1-4 from Erdaobaihe
Wangqing No. 1-3 from Wangqing County and Wangqing Forestry Bureau
Zuojia No. 1-3 from the Zuojia special production Institute of the Chinese Academy of Agricultural Sciences （CAAS）
Liaoning Huanren and Helong Qingshan
South Korea No. 1-4 from Chungbuk
Jeon- ham and Gyeongnam in South Korea. The similarity of RAPD markers of Jiaohe 2 and 3
the two wild species reached 98 % when the genetic distance was 0.0202. Almost all the wild species from the same area belonged to the same category within a very small genetic distance. For example
Antu No. 1-6 were within the 0.184 genetic distance
Jiaohe No. 1-5 were within 0.18 genetic distance
Wangqing No. 1-3 and Erdaobaihe No. 1 and No. 4 were within 0.315 genetic distance
Zuojia No. 1-3 was within 0.25 genetic distance
South Korea No. 1-4 were within 0.23 genetic distance
Erdaobaihe No. 2 and No. 3 were within 0.41 genetic distance. [Conclusion]There was a high genetic diversity among wild A. arguta collected from different geographic regions
but genetic diversity among those collected from the same geographical area was low. The genetic diversity of the wild A. arguta from South Korea was relatively low and was closely related to the wild A. arguta from Erdaobaihe
Wang Qing and Zuo Jia. The species collected from the etic diversity
RAPD
Changbai mountion
South Korea
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分类号
S663.4
[农业科学—果树学]
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