Oxygen uptake and transfer rates were studied in recombinant Pichia pastoris cultivation carried out in a stirred tank bioreactor.The oxygen uptake rate was determined by the macrokinetic model proposed earlier.Based ...Oxygen uptake and transfer rates were studied in recombinant Pichia pastoris cultivation carried out in a stirred tank bioreactor.The oxygen uptake rate was determined by the macrokinetic model proposed earlier.Based on pseudo-steady state mass transfer balance, an oxygen transfer model was established.Volumetric oxygen transfer coefficient k_La was estimated as a function of aeration rate and power input per unit volume.Oxygen uptake rate obtained from the macrokinetic model matched well with the oxygen transfer rate estimated by the mass balance model during the whole cultivation.The application of the oxygen transfer model for on-line biomass estimation was also presented.展开更多
为获得高产重组人血清白蛋白的转基因紫花苜蓿"游客"(Medicago sativa L. cv.‘Eureka’)株系用于规模化生产rHSA,构建了rHSA植物表达载体。以紫花苜蓿子叶愈伤组织为受体,通过农杆菌介导法进行遗传转化,筛选出潮霉素抗性植株...为获得高产重组人血清白蛋白的转基因紫花苜蓿"游客"(Medicago sativa L. cv.‘Eureka’)株系用于规模化生产rHSA,构建了rHSA植物表达载体。以紫花苜蓿子叶愈伤组织为受体,通过农杆菌介导法进行遗传转化,筛选出潮霉素抗性植株,提取抗性再生紫花苜蓿植株基因组DNA做PCR鉴定,结果表明重组人血清白蛋白基因片段已整合到紫花苜蓿基因组中;提取转基因植株总蛋白,Western blotting检测结果表明rHSA在转基因植株中成功表达。此结果表明转基因紫花苜蓿可稳定表达rHSA。展开更多
文摘Oxygen uptake and transfer rates were studied in recombinant Pichia pastoris cultivation carried out in a stirred tank bioreactor.The oxygen uptake rate was determined by the macrokinetic model proposed earlier.Based on pseudo-steady state mass transfer balance, an oxygen transfer model was established.Volumetric oxygen transfer coefficient k_La was estimated as a function of aeration rate and power input per unit volume.Oxygen uptake rate obtained from the macrokinetic model matched well with the oxygen transfer rate estimated by the mass balance model during the whole cultivation.The application of the oxygen transfer model for on-line biomass estimation was also presented.
文摘为获得高产重组人血清白蛋白的转基因紫花苜蓿"游客"(Medicago sativa L. cv.‘Eureka’)株系用于规模化生产rHSA,构建了rHSA植物表达载体。以紫花苜蓿子叶愈伤组织为受体,通过农杆菌介导法进行遗传转化,筛选出潮霉素抗性植株,提取抗性再生紫花苜蓿植株基因组DNA做PCR鉴定,结果表明重组人血清白蛋白基因片段已整合到紫花苜蓿基因组中;提取转基因植株总蛋白,Western blotting检测结果表明rHSA在转基因植株中成功表达。此结果表明转基因紫花苜蓿可稳定表达rHSA。