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Preliminary research and scheme design of deep underground in situ geo-information detection experiment for Geology in Time
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作者 Heping Xie Ru Zhang +13 位作者 Zetian Zhang Yinshuang Ai Jianhui Deng Yun Chen Yong Zhou Mingchuan Li Liqiang Liu Mingzhong Gao Zeqian Yang Weiqiang Ling Heng Gao Qijun Hao Kun Xiao Chendi Lou 《International Journal of Mining Science and Technology》 SCIE EI CAS CSCD 2024年第1期1-13,共13页
The deep earth,deep sea,and deep space are the main parts of the national“three deep”strategy,which is in the forefront of the strategic deployment clearly defined in China’s 14th Five-Year Plan(2021-2025)and the L... The deep earth,deep sea,and deep space are the main parts of the national“three deep”strategy,which is in the forefront of the strategic deployment clearly defined in China’s 14th Five-Year Plan(2021-2025)and the Long-Range Objectives Through the Year 2035.It is important to reveal the evolutionary process and mechanism of deep tectonics to understand the earth’s past,present and future.The academic con-notation of Geology in Time has been given for the first time,which refers to the multi-field evolution response process of geological bodies at different time and spatial scales caused by geological processes inside and outside the Earth.Based on the deep in situ detection space and the unique geological envi-ronment of China Jinping Underground Laboratory,the scientific issue of the correlation mechanism and law between deep internal time-varying and shallow geological response is given attention.Innovative research and frontier exploration on deep underground in situ geo-information detection experiments for Geology in Time are designed to be carried out,which will have the potential to explore the driving force of Geology in Time,reveal essential laws of deep earth science,and explore innovative technologies in deep underground engineering. 展开更多
关键词 Deep underground Geology in time China Jinping Underground Laboratory In situ detection
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Real time detection of antibody-antigen interaction using a laser scanning confocal imaging-surface plasmon resonance system 被引量:4
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作者 Zhang Hong-Yan Yang Li-Quan +5 位作者 Meng Lan Nie Jia-Cai Ning Ting-Yin Liu Wei-Min Sun Jia-Yu Wang Peng-Fei 《Chinese Physics B》 SCIE EI CAS CSCD 2012年第2期224-228,共5页
A laser scanning confocal imaging-surface plasmon resonance (LSCI-SPR) instrument integrated with a wavelength-dependent surface plasmon resonance (SPR) sensor and a laser scanning confocal microscopy (LSCM) is ... A laser scanning confocal imaging-surface plasmon resonance (LSCI-SPR) instrument integrated with a wavelength-dependent surface plasmon resonance (SPR) sensor and a laser scanning confocal microscopy (LSCM) is built to detect the bonding process of human IgG and fluorescent-labeled affinity purified antibodies in real time. The shifts of resonant wavelength at different reaction time stages are obtained by SPR, corresponding well with the changes of the fluorescence intensity collected by using LSCM. The instrument shows the merits of the combination and complementation of the SPR and LSCM, with such advantages as quantificational analysis, high spatial resolution and real time monitor, which are of great importance for practical applications in biosensor and life science. 展开更多
关键词 laser scanning confocal imaging-surface plasmon resonance COMBINATION COMPLEMENTATION real time
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Radiation dose detection using a high-power portable optically stimulated luminescence real-time reading system 被引量:2
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作者 Yi-Hang Wang Hua Chen +2 位作者 Fei Chen Yong-Gang Yuan Tai-Ping Peng 《Nuclear Science and Techniques》 SCIE CAS CSCD 2018年第10期245-253,共9页
Optically stimulated luminescence(OSL) reading systems are becoming smaller and capable of real-time detection. To improve real-time and multipurpose radiation dosimetry readings, we built a real-time continuous-wave(... Optically stimulated luminescence(OSL) reading systems are becoming smaller and capable of real-time detection. To improve real-time and multipurpose radiation dosimetry readings, we built a real-time continuous-wave(RCW) OSL reading system. This system is both small and lightweight, and it employs powerful laser excitation(478 mW/cm^2) at the dosimetry probe location. We investigate the possibility of using the RCW mode to read the radiation luminescence(RL) or OSL by using a singlecrystal Al_2O_3:C dosimeter in a low-dose-rate137 Cs y field.Our results indicate that the RL/OSL follows a stable and uniform distribution. The minimum detected doses associated with the RL, OSL, and RL + OSL signals are2.1 9 10^(-2), 3.17 9 10^(-1), and 5.7 9 10^(-2) l Gy, respectively. This device provides a framework for the future development of applications for practical radiation dose measurements. 展开更多
关键词 OSL real-time READING Al2O3:C Radiation DOSE
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Real-Time Detection for Magnetic Island of Neoclassical Tearing Mode in EAST Plasma Control System
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作者 梁绍勇 肖炳甲 +5 位作者 张洋 王淋芳 袁旗平 罗正平 石同辉 提昂 《Plasma Science and Technology》 SCIE EI CAS CSCD 2016年第2期197-201,共5页
Accurate detection of a magnetic island in real time is one of the important issues for the tearing mode(TM) and neoclassical tearing mode(NTM) control.This paper presents a real-time detection system for the magn... Accurate detection of a magnetic island in real time is one of the important issues for the tearing mode(TM) and neoclassical tearing mode(NTM) control.This paper presents a real-time detection system for the magnetic island of NTM control in the EAST Plasma Control System(PCS).Diagnosis is based on magnetic periodic perturbation and electron temperature fluctuation caused by the magnetic island.Therefore,a Mirnov measurement has been selected to calculate the island's parameters,such as island width,frequency of island rotation,and toroidal number.The electron cyclotron emission(ECE) system can detect the island position,which is calculated by two fast detection algorithms called correlation analysis and Hilbert transform.For future NTM control,real-time equilibrium reconstruction(rt-EFIT) is needed to locate the rational q-surface where the island is detected.This fast detection system is able to detect an island within 3 ms.It can be integrated into PCS to provide effective parameters of the island for NTM control by using EC resonance heating(ECRH) in the next experiment of EAST. 展开更多
关键词 real time magnetic island Mirnov ECE phase jump
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4种植物源性成分多重real-time PCR检测方法的建立及其在食用淀粉中的应用 被引量:3
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作者 范维 高晓月 +4 位作者 董雨馨 刘虹宇 李贺楠 赵文涛 郭文萍 《食品科学》 EI CAS CSCD 北大核心 2024年第1期210-216,共7页
建立一种可同时快速检测红薯、木薯、马铃薯、玉米源性成分的多重实时聚合酶链式反应(real-time polymerase chain reaction,real-time PCR)方法。分别以红薯g3pdh基因、木薯g3pdh基因、马铃薯UGPase基因、玉米zSSIIb基因为靶基因设计... 建立一种可同时快速检测红薯、木薯、马铃薯、玉米源性成分的多重实时聚合酶链式反应(real-time polymerase chain reaction,real-time PCR)方法。分别以红薯g3pdh基因、木薯g3pdh基因、马铃薯UGPase基因、玉米zSSIIb基因为靶基因设计特异性引物和TaqMan探针,以18S rRNA基因为内参基因,建立多重real-time PCR方法,开展方法学验证,并对不同掺入比例模拟样品和实际淀粉样品进行检测。结果显示,该方法具有高通量、特异性强、灵敏度高等优点。与15种非目标源性均无交叉反应;对目标DNA的检测灵敏度可达到3×10^(-3) ng/μL,且具有良好的线性关系和扩增效率;对淀粉样品的检出限可达0.1%,对50份实际样品进行检测,结果与参比方法一致,说明建立的多重real-time PCR法可用于食用淀粉种类掺假鉴别检测。 展开更多
关键词 多重实时聚合酶链式反应 食用淀粉 木薯 红薯 马铃薯 玉米
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Building Real-Time Network Intrusion Detection System Based on Parallel Time-Series Mining Techniques
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作者 赵峰 李庆华 《Journal of Southwest Jiaotong University(English Edition)》 2005年第1期11-17,共7页
A new real-time model based on parallel time-series mining is proposed to improve the accuracy and efficiency of the network intrusion detection systems. In this model, multidimensional dataset is constructed to descr... A new real-time model based on parallel time-series mining is proposed to improve the accuracy and efficiency of the network intrusion detection systems. In this model, multidimensional dataset is constructed to describe network events, and sliding window updating algorithm is used to maintain network stream. Moreover, parallel frequent patterns and frequent episodes mining algorithms are applied to implement parallel time-series mining engineer which can intelligently generate rules to distinguish intrusions from normal activities. Analysis and study on the basis of DAWNING 3000 indicate that this parallel time-series mining-based model provides a more accurate and efficient way to building real-time NIDS. 展开更多
关键词 Intrusion detection time-series mining Sliding window Parallel algorithm
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基于病菌孢子捕捉和real-time PCR技术的田间空气中小麦白粉病菌孢子动态监测及病情估计模型研究 被引量:1
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作者 王奥霖 商昭月 +8 位作者 张美惠 王贵 胡小平 徐飞 孙振宇 曹世勤 刘伟 范洁茹 周益林 《植物保护》 CAS CSCD 北大核心 2024年第2期49-56,72,共9页
利用Burkard定容式孢子捕捉器结合real-time PCR定量技术,分别对种植高抗、中感和高感白粉病小麦品种的田间空气中白粉病菌分生孢子浓度进行监测,结果表明,real-time PCR定量与传统的显微观察计数两种方法测得的孢子浓度呈显著正相关(P... 利用Burkard定容式孢子捕捉器结合real-time PCR定量技术,分别对种植高抗、中感和高感白粉病小麦品种的田间空气中白粉病菌分生孢子浓度进行监测,结果表明,real-time PCR定量与传统的显微观察计数两种方法测得的孢子浓度呈显著正相关(P≤0.01),且两种病菌孢子计数方法在同一抗性品种上监测到的孢子浓度动态相近。此外,两种方法测得的孢子浓度与各气象因子的相关性分析结果一致,空气中的白粉病菌孢子浓度主要与空气相对湿度显著正相关。在此基础上,利用两种方法测定的田间空气中白粉病菌孢子浓度分别建立了基于累积孢子浓度的田间病情估计模型。分析发现,基于两种孢子浓度测定方法建立的病情估计模型间无显著性差异,表明real-time PCR定量技术测定的孢子浓度在构建白粉病病情估计模型上具有一定可行性。该结果为real-time PCR定量技术与病菌孢子捕捉技术相结合用于小麦白粉病的监测和预测提供理论依据。 展开更多
关键词 小麦白粉病 病菌孢子捕捉 实时荧光定量PCR 病原菌监测 病情估计模型
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基于Real-time PCR法检测乳粉中牛源性成分定量研究
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作者 陈晨 史国华 +5 位作者 陈勃旭 张瑞 王玉欣 贾文珅 陈佳 周巍 《粮油食品科技》 CAS CSCD 北大核心 2024年第2期159-164,共6页
基于Real-timePCR建立了乳粉中牛源性成分相对定量检测方法,并对牛的特异性引物与探针进行了特异性、灵敏度和稳定性测试。通过模拟不同浓度牛乳粉与马乳粉混合样本,根据其△Ct值的函数关系进行线性拟合进而绘制标准曲线,建立乳粉中牛... 基于Real-timePCR建立了乳粉中牛源性成分相对定量检测方法,并对牛的特异性引物与探针进行了特异性、灵敏度和稳定性测试。通过模拟不同浓度牛乳粉与马乳粉混合样本,根据其△Ct值的函数关系进行线性拟合进而绘制标准曲线,建立乳粉中牛源性成分的相对定量检测。结果显示,该方法的最低检测限为0.00001 mg/mL,回收率为91.11%~119.2%,组间变异系数≤0.58%、组内变异系数≤1.44%。说明该方法在特异性与稳定性上适用于乳粉中牛源性成分及含量的掺假检测。 展开更多
关键词 牛乳粉 马乳粉 real-time PCR 掺假检测
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马乳酒样乳杆菌马乳酒样亚种real-time PCR检测方法的建立与应用
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作者 吕厚姣 李欣媛 +3 位作者 白小佳 贾龙刚 耿伟涛 王艳萍 《食品科学》 EI CAS CSCD 北大核心 2024年第9期102-108,共7页
本研究建立了一种特异性实时聚合酶链式反应(real-time polymerase chain reaction,real-time PCR)检测方法,根据模式菌株马乳酒样乳杆菌马乳酒样亚种ZW3的16S rDNA序列和全基因组序列设计筛选特异性引物,采用SYBR Green I荧光染料建立r... 本研究建立了一种特异性实时聚合酶链式反应(real-time polymerase chain reaction,real-time PCR)检测方法,根据模式菌株马乳酒样乳杆菌马乳酒样亚种ZW3的16S rDNA序列和全基因组序列设计筛选特异性引物,采用SYBR Green I荧光染料建立real-time PCR方法,并对方法的特异性、灵敏度、重复性和混合体系等进行检测。结果表明,本研究所建立的方法特异性强、灵敏度高、重复性好,建立real-time PCR的标准曲线,其决定系数R2为0.965,具有良好的线性关系,且在马乳酒样乳杆菌马乳酒样亚种及混合体系中可以特异性检出。综上,本研究建立的real-time PCR法可以快速、准确地检测马乳酒样乳杆菌马乳酒样亚种,为马乳酒样乳杆菌的特异性定性定量检测提供了一种新的方法。 展开更多
关键词 马乳酒样乳杆菌马乳酒样亚种 实时聚合酶链式反应 特异性引物
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Digital coherent detection research on Brillouin optical time domain reflectometry with simplex pulse codes 被引量:7
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作者 郝蕴琦 叶青 +2 位作者 潘政清 蔡海文 瞿荣辉 《Chinese Physics B》 SCIE EI CAS CSCD 2014年第11期253-256,共4页
The digital coherent detection technique has been investigated without any frequency-scanning device in the Brillouin optical time domain reflectometry (BOTDR), where the simplex pulse codes are applied in the sensi... The digital coherent detection technique has been investigated without any frequency-scanning device in the Brillouin optical time domain reflectometry (BOTDR), where the simplex pulse codes are applied in the sensing system. The time domain signal of every code sequence is collected by the data acquisition card (DAQ). A shift-averaging technique is applied in the frequency domain for the reason that the local oscillator (LO) in the coherent detection is fix-frequency deviated from the primary source. With the 31-bit simplex code, the signal-to-noise ratio (SNR) has 3.5-dB enhancement with the same single pulse traces, accordant with the theoretical analysis. The frequency fluctuation for simplex codes is 14.01 MHz less than that for a single pulse as to 4-m spatial resolution. The results are believed to be beneficial for the BOTDR performance improvement. 展开更多
关键词 Brillouin optical time domain reflectometry digital coherent detection simplex pulse codes signal-to-noise ratio
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Real-Time Method for Detecting Harmonic and Reactive Currents of Single-Phase Circuits 被引量:1
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作者 张秀峰 丁菊霞 《Journal of Southwest Jiaotong University(English Edition)》 2006年第2期135-141,共7页
According to the characteristics of single-phase circuits and demand of using active filter for real-time detecting harmonic and reactive currents, a detecting method based on Fryze's power definition is proposed. Th... According to the characteristics of single-phase circuits and demand of using active filter for real-time detecting harmonic and reactive currents, a detecting method based on Fryze's power definition is proposed. The results of theoretical analysis and simula- tion show that the proposed method is effective in realtime detecting of instantaneous harmonic and reactive currents in single-phase circuits. When only detecting the total reactive currents, this method does not need a phase-locked loop circuit, and it also can be used in some special applications to provide different compensations on the ground of different requirements of electric network. Compared with the other methods based on the theory of instantaneous reactive power, this method is simple and easy to realize. 展开更多
关键词 Active filter HARMONIC Reactive current real-time detection Single-phase circuit Electric-network
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24重荧光real-time PCR技术在食物中毒快速检测中的应用
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作者 卢媛 钟颖涛 《食品安全导刊》 2024年第6期85-88,共4页
目的:应用24重荧光real-time PCR检测技术快速筛检食物中毒病原菌,结合国家标准中的培养法探讨24重荧光real-time PCR检测技术符合性和应用价值。方法:采用高灵敏度、高特异性的24重荧光real-time PCR检测技术作为中毒病原菌的初筛方法... 目的:应用24重荧光real-time PCR检测技术快速筛检食物中毒病原菌,结合国家标准中的培养法探讨24重荧光real-time PCR检测技术符合性和应用价值。方法:采用高灵敏度、高特异性的24重荧光real-time PCR检测技术作为中毒病原菌的初筛方法,国标方法进行细菌分离培养,并对分离出的病原菌进行生化鉴定。结果:5份食物中毒患者肛拭子在增菌前检出4份霍乱弧菌核酸(非O1/非O139群,24重荧光real-time PCR),9份患者肛拭子在增菌后检出5株霍乱弧菌(非O1/非O139群,国标培养法),其中包含4份PCR技术初筛阳性样品,两个方法的符合率为80%。所有样本均未检出沙门氏菌、志贺菌、副溶血性弧菌、致泻性大肠埃希菌以及金黄色葡萄球菌。结论:应用24重荧光real-time PCR检测技术同时检测24种常见致病病原菌,能高效锁定中毒病原菌。将其与国标培养法相结合,对临床治疗和食物中毒快速处置能起到积极作用,值得应用和推广。 展开更多
关键词 24重荧光real-time PCR技术 培养法 食物中毒
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A Space-Time Reverberation Model for Moving Target Detection 被引量:1
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作者 Jingwei Yin Bing Liu +1 位作者 Guangping Zhu Xiao Han 《Journal of Marine Science and Application》 CSCD 2019年第4期522-529,共8页
In recent years,moving target detection methods based on low-rank and sparse matrix decomposition have been developed,and they have achieved good results.However,there is not enough interpretation to support the assum... In recent years,moving target detection methods based on low-rank and sparse matrix decomposition have been developed,and they have achieved good results.However,there is not enough interpretation to support the assumption that there is a high correlation among the reverberations after each transmitting pulse.In order to explain the correlation of reverberations,a new reverberation model is proposed from the perspective of scattering cells in this paper.The scattering cells are the subarea divided from the detection area.The energy fluctuation of a scattering cell with time and the influence of the neighboring cells are considered.Key parameters of the model were analyzed by numerical analysis,and the applicability of the model was verified by experimental analysis.The results showed that the model can be used for several simulations to evaluate the performance of moving target detection methods. 展开更多
关键词 Space-time reverberation Model scattering cell Energy fluctuation Moving target detection
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Detection of K in soil using time-resolved laser-induced breakdown spectroscopy based on convolutional neural networks 被引量:1
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作者 Chengxu LU Bo WANG +3 位作者 Xunpeng JIANG Junning ZHANG Kang NIU Yanwei YUAN 《Plasma Science and Technology》 SCIE EI CAS CSCD 2019年第3期108-113,共6页
One of the technical bottlenecks of traditional laser-induced breakdown spectroscopy(LIBS) is the difficulty in quantitative detection caused by the matrix effect. To troubleshoot this problem,this paper investigated ... One of the technical bottlenecks of traditional laser-induced breakdown spectroscopy(LIBS) is the difficulty in quantitative detection caused by the matrix effect. To troubleshoot this problem,this paper investigated a combination of time-resolved LIBS and convolutional neural networks(CNNs) to improve K determination in soil. The time-resolved LIBS contained the information of both wavelength and time dimension. The spectra of wavelength dimension showed the characteristic emission lines of elements, and those of time dimension presented the plasma decay trend. The one-dimensional data of LIBS intensity from the emission line at 766.49 nm were extracted and correlated with the K concentration, showing a poor correlation of R_c^2?=?0.0967, which is caused by the matrix effect of heterogeneous soil. For the wavelength dimension, the two-dimensional data of traditional integrated LIBS were extracted and analyzed by an artificial neural network(ANN), showing R_v^2?=?0.6318 and the root mean square error of validation(RMSEV)?=?0.6234. For the time dimension, the two-dimensional data of time-decay LIBS were extracted and analyzed by ANN, showing R_v^2?=?0.7366 and RMSEV?=?0.7855.These higher determination coefficients reveal that both the non-K emission lines of wavelength dimension and the spectral decay of time dimension could assist in quantitative detection of K.However, due to limited calibration samples, the two-dimensional models presented over-fitting.The three-dimensional data of time-resolved LIBS were analyzed by CNNs, which extracted and integrated the information of both the wavelength and time dimension, showing the R_v^2?=?0.9968 and RMSEV?=?0.0785. CNN analysis of time-resolved LIBS is capable of improving the determination of K in soil. 展开更多
关键词 quantitative detection potassium(K) SOIL time-RESOLVED LASER-INDUCED breakdown spectroscopy(LIBS) convolutional neural networks(CNNs)
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Community detection in signed networks based on discrete-time model
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作者 Jianrui Chen Li Zhang +1 位作者 Weiwei Liu Zaizai Yan 《Chinese Physics B》 SCIE EI CAS CSCD 2017年第1期574-583,共10页
Community detection in signed networks has been studied widely in recent years. In this paper, a discrete difference equation is proposed to imitate the consistently changing phases of the nodes. During the interactio... Community detection in signed networks has been studied widely in recent years. In this paper, a discrete difference equation is proposed to imitate the consistently changing phases of the nodes. During the interaction, each node will update its phase based on the difference equation. Each node has many different nodes connected with it, and these neighbors have different influences on it. The similarity between two nodes is applied to describe the influences between them. Nodes with high positive similarities will get together and nodes with negative similarities will be far away from each other.Communities are detected ultimately when the phases of the nodes are stable. Experiments on real world and synthetic signed networks show the efficiency of detection performance. Moreover, the presented method gains better detection performance than two existing good algorithms. 展开更多
关键词 community detection signed networks discrete-time model SIMILARITY
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Frequency response range of terahertz pulse coherent detection based on THz-induced time-resolved luminescence quenching
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作者 Man Zhang Zhen-Gang Yang +3 位作者 Jin-Song Liu Ke-Jia Wang Jiao-Li Gong Sheng-Lie Wang 《Chinese Physics B》 SCIE EI CAS CSCD 2018年第6期127-131,共5页
It has been proposed previously that the coherent detection of a terahertz(THz) pulse can be achieved based on the time-resolved luminescence quenching. In this paper, we investigate the frequency response range of ... It has been proposed previously that the coherent detection of a terahertz(THz) pulse can be achieved based on the time-resolved luminescence quenching. In this paper, we investigate the frequency response range of this novel detection technology by simulating the motion of carriers in gallium arsenide(GaAs) by the ensemble Monte Carlo method. At room temperature, for a direct-current(DC) voltage of 20 kV/cm applied to the semiconductor(GaAs) and sampling time o140 fs, the luminescence quenching phenomena induced by terahertz pulses with different center frequencies are studied The results show that the quenching efficiency is independent of the THz frequency when the frequency is in a range o0.1 THz–4 THz. However, when the frequency exceeds 4 THz, the efficiency decreases with the increase of frequency Therefore, the frequency response range is 0.1 THz–4 THz. Moreover, when the sampling time is changed to 100 fs the frequency response range is extended to be approximately 0.1 THz–5.6 THz. This study of the frequency-dependen characteristics of the luminescence response to the THz pulse can provide a theoretical basis for the exploration of THz detection technology. 展开更多
关键词 frequency response range terahertz-pulse coherent detection time-resolved luminescence quenching ensemble Monte Carlo method
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转基因番茄植株Mi1.2表达水平的Real Time PCR检测体系的建立 被引量:3
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作者 魏振林 田志环 +1 位作者 焦传珍 董玲 《安徽农业科学》 CAS 北大核心 2008年第18期7589-7590,共2页
[目的]为转基因番茄植株的高通量筛选奠定基础。[方法]利用CTAB法提取番茄叶片总RNA进行Real Time PCR扩增,分析转MI1、2基因的番茄植株表达水平的检测体系。[结果]提取RNA的A260/A280为1.78~1.88,RNA无明显降解。在严谨扩增条件... [目的]为转基因番茄植株的高通量筛选奠定基础。[方法]利用CTAB法提取番茄叶片总RNA进行Real Time PCR扩增,分析转MI1、2基因的番茄植株表达水平的检测体系。[结果]提取RNA的A260/A280为1.78~1.88,RNA无明显降解。在严谨扩增条件下,引物SYBR2的扩增效率高于SYBR1。Mg^2+的适宜浓度为2.0mg/L。Real Time PCR扩增产物具有良好的特异性,熔解曲线特异峰出现在84.5℃附近,在熔解曲线略低于83℃附近有极微弱的非特异峰。因此在定量反应中信号检测步骤应放在84℃。以4种不同模板分子数条件下扩增曲线Ct值得到的回归方程为Y=-3.78×log(copynumber)+39.50,相关系数为0.998。[结论]该试验获得的Real Time PCR体系可用于转基因植株表达水平的检测。 展开更多
关键词 real time PCR 检测体系 转基因番茄植株 熔解曲线
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Real-time PCR及预测微生物学在白酒酿造中的应用构想 被引量:7
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作者 孙超 卢慧丽 刘勇 《中国酿造》 CAS 2012年第10期7-10,共4页
利用实时荧光定量PCR技术及预测微生物学建立可培养酿酒微生物资源数据库和预测微生物学数学模型,可探究在白酒酿造过程中可培养酿酒微生物的变化规律及不同环境因素对可培养酿酒微生物菌群变化的影响,为白酒酿造工艺优化提供重要参数。
关键词 realtime PCR 预测微生物学 白酒酿造
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一种5重real-time PCR筛查转基因水稻方法的建立 被引量:6
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作者 董立明 杨帆 +4 位作者 邢珍娟 李葱葱 闫伟 龙丽坤 李飞武 《食品科学》 EI CAS CSCD 北大核心 2021年第24期329-334,共6页
以转基因水稻中最常用的CaMV35S启动子、NOS终止子、Cry1Ab/Ac基因、HPT基因及SPS水稻内标基因为研究对象,利用5种不同的荧光信号(FAM、HEX、Taxas Red、Cy5、Cy5.5)进行多重实时聚合酶链式反应(realtime polymerase chain reaction,rea... 以转基因水稻中最常用的CaMV35S启动子、NOS终止子、Cry1Ab/Ac基因、HPT基因及SPS水稻内标基因为研究对象,利用5种不同的荧光信号(FAM、HEX、Taxas Red、Cy5、Cy5.5)进行多重实时聚合酶链式反应(realtime polymerase chain reaction,real-time PCR)检测方法的研究。通过引物组合筛选、反应体系优化、特异性测试、灵敏度测试、适用性测试等一系列实验,建立了5重real-time PCR方法,灵敏度可达0.032%。此方法具有灵敏度高、结果准确、通量大等优点,可实现水稻中转基因成分的快速、高效检测。 展开更多
关键词 转基因水稻 筛查元件 单一实时聚合酶链式反应 5重实时聚合酶链式反应 高通量检测方法
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应用SYBR GreenI的Real-time PCR方法定量检测空肠弯曲杆菌 被引量:10
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作者 阳成波 蒋原 +1 位作者 黄克和 祝长青 《动物医学进展》 CSCD 2003年第1期74-78,共5页
空肠弯曲杆菌(Campylobacterjejuni)被认为是人类主要食源性病原菌之一。由于空肠弯曲杆菌特殊的生长条件和容易进入不可培养但存活的状态(Viablebutnonculturable,VNC),所以传统的生化鉴定结果不一定可靠,并且是一项费时而繁琐的工作... 空肠弯曲杆菌(Campylobacterjejuni)被认为是人类主要食源性病原菌之一。由于空肠弯曲杆菌特殊的生长条件和容易进入不可培养但存活的状态(Viablebutnonculturable,VNC),所以传统的生化鉴定结果不一定可靠,并且是一项费时而繁琐的工作。核酸检测方法的出现为空肠弯曲杆菌的检测带来了方便。本文基于LightCycler为平台,利用SYBRGreenI染料能特异与双链DNA结合而发荧光的特性,建立一种Real-timePCR方法来定量检测空肠弯曲杆菌。用该方法检测时,发现所有空肠弯曲杆菌(11株)都呈阳性,所有其它弯曲菌(3株)和其它菌株(5株)都是阴性。整个检测过程60min内可以完成,检测限度为5CFU,标准曲线的相关系数为1。结果表明基于SYBRGreenI的定量PCR方法既为空肠弯曲杆菌提供了一种特异、敏感、快速和简洁的定量检测方法,又为研究空肠弯曲杆菌致病机理提供了一种重要方法。 展开更多
关键词 空肠弯曲杆菌 定量检测 SYBRGreenI染料 real-timePCR方法 致病机理 食源性病原菌
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