In order to optimize the embedded system implementation for Ethernet-based computer numerical control （CNC） system, it is very necessary to establish the performance analysis model and further adopt the codesign met...In order to optimize the embedded system implementation for Ethernet-based computer numerical control （CNC） system, it is very necessary to establish the performance analysis model and further adopt the codesign method from the control, communication and computing perspectives. On the basis of analyzing real-time Ethemet, system architecture, time characteristic parameters of control-loop ere, a performance analysis model for real-time Ethemet-based CNC system was proposed, which is able to include the timing effects caused by the implementation platform in the simulation. The key for establishing the model is accomplished by designing the error analysis module and the controller nodes. Under the restraint of CPU resource and communication bandwidth, the experiment with a case study was conducted, and the results show that if the deadline miss ratio of data packets is 0.2%, then the percentage error is 1.105%. The proposed model can be used at several stages of CNC system development.展开更多
A novel reconfigurable hardware system which uses both muhi-DSP and FPGA to attain high performance and real-time image processing are presented. The system structure and working principle of mainly processing multi-B...A novel reconfigurable hardware system which uses both muhi-DSP and FPGA to attain high performance and real-time image processing are presented. The system structure and working principle of mainly processing multi-BSP board, extended multi-DSP board are analysed. The outstanding advantage is that the communication among different board components of this system is supported by high speed link ports ＆ serial ports for increasing the system performance and computational power. Then the implementation of embedded real-time operating systems （RTOS） by us is discussed in detail. In this system, we adopt two kinds of parallel structures controlled by RTOS for parallel processing of algorithms. The experimental results show that exploitive period of the system is short, and maintenance convenient. Thus it is suitable for real-time image processing and can get satisfactory effect of image recognition.展开更多
A newly designed approach of simultaneous stabilization is given for linear discrete time-delay systems. The problem of stabilization for a collection of systems is discussed initially. Adequate condition are obtained...A newly designed approach of simultaneous stabilization is given for linear discrete time-delay systems. The problem of stabilization for a collection of systems is discussed initially. Adequate condition are obtained in terms of linear matrix inequalities （LMIs） which are independent of time delays such that the resultant collection of discrete time-delay systems are stable with an upper bound of the quadratic performance index. Subsequently, controllers are designed such that the resultant closed-loop discrete time-delay systems are simultaneously stabilized with the upper bound of the quadratic performance index. Finally,a numerical example is given to illustrate the design method.展开更多
[目的]为转基因番茄植株的高通量筛选奠定基础。[方法]利用CTAB法提取番茄叶片总RNA进行Real Time PCR扩增，分析转MI1、2基因的番茄植株表达水平的检测体系。[结果]提取RNA的A260／A280为1．78～1．88，RNA无明显降解。在严谨扩增条件...[目的]为转基因番茄植株的高通量筛选奠定基础。[方法]利用CTAB法提取番茄叶片总RNA进行Real Time PCR扩增，分析转MI1、2基因的番茄植株表达水平的检测体系。[结果]提取RNA的A260／A280为1．78～1．88，RNA无明显降解。在严谨扩增条件下，引物SYBR2的扩增效率高于SYBR1。Mg^2＋的适宜浓度为2．0mg／L。Real Time PCR扩增产物具有良好的特异性，熔解曲线特异峰出现在84．5℃附近，在熔解曲线略低于83℃附近有极微弱的非特异峰。因此在定量反应中信号检测步骤应放在84℃。以4种不同模板分子数条件下扩增曲线Ct值得到的回归方程为Y=-3．78×log（copynumber）＋39．50，相关系数为0．998。[结论]该试验获得的Real Time PCR体系可用于转基因植株表达水平的检测。展开更多
为探讨PRRSV感染后机体的体液免疫应答、从分子水平深入研究PRRSV的免疫机制,本研究针对Th2型细胞因子IL-4、IL-6、IL-10以及看家基因β-actin的基因序列分别设计一对特异性引物,构建含有各自引物扩增序列的重组质粒作为阳性标准品,建...为探讨PRRSV感染后机体的体液免疫应答、从分子水平深入研究PRRSV的免疫机制,本研究针对Th2型细胞因子IL-4、IL-6、IL-10以及看家基因β-actin的基因序列分别设计一对特异性引物,构建含有各自引物扩增序列的重组质粒作为阳性标准品,建立了检测IL-4、IL-6、IL-10及β-actin的SYBR Green Ⅰ real-time PCR方法。该方法线性关系好,各种细胞因子及β-actin标准曲线的相关系数均达到0.997以上;敏感性高,初始模板的检出下限均达到1×101copies/μL;特异性强,扩增产物形成单一的特异性熔解峰;重复性好,组内与组间的变异系数均小于3%。应用所建立的方法对猪繁殖与呼吸综合征病毒(PRRSV)感染仔猪外周血单个核细胞(PBMC)中IL-4、IL-6和IL-10mRNA的表达水平进行了检测。结果表明,本研究建立的real-timePCR检测方法灵敏度高、特异性强、重复性好,可以用于猪Th2型细胞因子的检测及定量分析。展开更多
基金Projects(50875090,50905063) supported by the National Natural Science Foundation of ChinaProject(2009AA04Z111) supported by the National High Technology Research and Development Program of China+2 种基金Project(20090460769) supported by China Postdoctoral Science FoundationProject(2011ZM0070) supported by the Fundamental Research Funds for the Central Universities in ChinaProject(S2011010001155) supported by the Natural Science Foundation of Guangdong Province,China
文摘In order to optimize the embedded system implementation for Ethernet-based computer numerical control （CNC） system, it is very necessary to establish the performance analysis model and further adopt the codesign method from the control, communication and computing perspectives. On the basis of analyzing real-time Ethemet, system architecture, time characteristic parameters of control-loop ere, a performance analysis model for real-time Ethemet-based CNC system was proposed, which is able to include the timing effects caused by the implementation platform in the simulation. The key for establishing the model is accomplished by designing the error analysis module and the controller nodes. Under the restraint of CPU resource and communication bandwidth, the experiment with a case study was conducted, and the results show that if the deadline miss ratio of data packets is 0.2%, then the percentage error is 1.105%. The proposed model can be used at several stages of CNC system development.
基金This project was supported by the National Natural Science Foundation of China(60135020) National Key Pre-researchProject of China(413010701 -3) .
文摘A novel reconfigurable hardware system which uses both muhi-DSP and FPGA to attain high performance and real-time image processing are presented. The system structure and working principle of mainly processing multi-BSP board, extended multi-DSP board are analysed. The outstanding advantage is that the communication among different board components of this system is supported by high speed link ports ＆ serial ports for increasing the system performance and computational power. Then the implementation of embedded real-time operating systems （RTOS） by us is discussed in detail. In this system, we adopt two kinds of parallel structures controlled by RTOS for parallel processing of algorithms. The experimental results show that exploitive period of the system is short, and maintenance convenient. Thus it is suitable for real-time image processing and can get satisfactory effect of image recognition.
基金This project was Supported by the National Natural Science Foundation of China (50335020,60574011) PostdoctoralFund (2005038553) Science Research Important Foundation in Hubei Provincial Department of Education(2002z04001).
文摘A newly designed approach of simultaneous stabilization is given for linear discrete time-delay systems. The problem of stabilization for a collection of systems is discussed initially. Adequate condition are obtained in terms of linear matrix inequalities （LMIs） which are independent of time delays such that the resultant collection of discrete time-delay systems are stable with an upper bound of the quadratic performance index. Subsequently, controllers are designed such that the resultant closed-loop discrete time-delay systems are simultaneously stabilized with the upper bound of the quadratic performance index. Finally,a numerical example is given to illustrate the design method.
文摘[目的]为转基因番茄植株的高通量筛选奠定基础。[方法]利用CTAB法提取番茄叶片总RNA进行Real Time PCR扩增，分析转MI1、2基因的番茄植株表达水平的检测体系。[结果]提取RNA的A260／A280为1．78～1．88，RNA无明显降解。在严谨扩增条件下，引物SYBR2的扩增效率高于SYBR1。Mg^2＋的适宜浓度为2．0mg／L。Real Time PCR扩增产物具有良好的特异性，熔解曲线特异峰出现在84．5℃附近，在熔解曲线略低于83℃附近有极微弱的非特异峰。因此在定量反应中信号检测步骤应放在84℃。以4种不同模板分子数条件下扩增曲线Ct值得到的回归方程为Y=-3．78×log（copynumber）＋39．50，相关系数为0．998。[结论]该试验获得的Real Time PCR体系可用于转基因植株表达水平的检测。
文摘为探讨PRRSV感染后机体的体液免疫应答、从分子水平深入研究PRRSV的免疫机制,本研究针对Th2型细胞因子IL-4、IL-6、IL-10以及看家基因β-actin的基因序列分别设计一对特异性引物,构建含有各自引物扩增序列的重组质粒作为阳性标准品,建立了检测IL-4、IL-6、IL-10及β-actin的SYBR Green Ⅰ real-time PCR方法。该方法线性关系好,各种细胞因子及β-actin标准曲线的相关系数均达到0.997以上;敏感性高,初始模板的检出下限均达到1×101copies/μL;特异性强,扩增产物形成单一的特异性熔解峰;重复性好,组内与组间的变异系数均小于3%。应用所建立的方法对猪繁殖与呼吸综合征病毒(PRRSV)感染仔猪外周血单个核细胞(PBMC)中IL-4、IL-6和IL-10mRNA的表达水平进行了检测。结果表明,本研究建立的real-timePCR检测方法灵敏度高、特异性强、重复性好,可以用于猪Th2型细胞因子的检测及定量分析。
