This experiment designed the pulsed electric fields （PEF） of high intensity of 100 kV· cm^-1 sterilization system. Fluorescent pseudomonas as target cell was operated 180 s in the PEF. By observing the differen...This experiment designed the pulsed electric fields （PEF） of high intensity of 100 kV· cm^-1 sterilization system. Fluorescent pseudomonas as target cell was operated 180 s in the PEF. By observing the difference of the bacteria before and after the disposal by TEM, it is found that the cell walls of the treated bacteria were broken. Irreversible perforations were formed on the cell membrane. The cell inclusions and cell fragments were leaked. The cell died as a result. The results showed that the PEF sterilization system designed can be used for liquid food sterilization experiments.展开更多
甜菜是我国制得蔗糖的主要原料之一,而甜菜制糖的传统热水提取法存在提取时间长、能耗高、提取率低等问题,不符合绿色环保的可持续发展理念。脉冲电场(pulsed electric field,PEF)辅助提取被公认为一种可靠的提取手段,具有短时、非热、...甜菜是我国制得蔗糖的主要原料之一,而甜菜制糖的传统热水提取法存在提取时间长、能耗高、提取率低等问题,不符合绿色环保的可持续发展理念。脉冲电场(pulsed electric field,PEF)辅助提取被公认为一种可靠的提取手段,具有短时、非热、高效等特点,用于甜菜制糖已有不少研究。该文概述国内外使用PEF预处理提取甜菜糖的相关应用,介绍其辅助提取的作用机制和影响提取率的主要因素,并对PEF提取应用做分析与展望,以期为实际生产提供理论指导。展开更多
基金the Youth Science Fund of Heilongjiang Province (QC07C39)Postdoctoral Fund of Heilongjiang (LBH-Z06163)Science Research Fund of Northeast Agricultural University
文摘This experiment designed the pulsed electric fields （PEF） of high intensity of 100 kV· cm^-1 sterilization system. Fluorescent pseudomonas as target cell was operated 180 s in the PEF. By observing the difference of the bacteria before and after the disposal by TEM, it is found that the cell walls of the treated bacteria were broken. Irreversible perforations were formed on the cell membrane. The cell inclusions and cell fragments were leaked. The cell died as a result. The results showed that the PEF sterilization system designed can be used for liquid food sterilization experiments.
文摘甜菜是我国制得蔗糖的主要原料之一,而甜菜制糖的传统热水提取法存在提取时间长、能耗高、提取率低等问题,不符合绿色环保的可持续发展理念。脉冲电场(pulsed electric field,PEF)辅助提取被公认为一种可靠的提取手段,具有短时、非热、高效等特点,用于甜菜制糖已有不少研究。该文概述国内外使用PEF预处理提取甜菜糖的相关应用,介绍其辅助提取的作用机制和影响提取率的主要因素,并对PEF提取应用做分析与展望,以期为实际生产提供理论指导。
