用高效阴离子交换色谱-脉冲安培检测法(HPAEC-PAD)测定了烤烟中的水溶性葡萄糖、果糖和蔗糖。采用水浸取及膜过滤法处理烤烟样品,以D ionex C arboPac PA-1阴离子交换柱为色谱柱,0.2m o l/L N aOH水溶液为淋洗液进行分离测定。葡萄糖、...用高效阴离子交换色谱-脉冲安培检测法(HPAEC-PAD)测定了烤烟中的水溶性葡萄糖、果糖和蔗糖。采用水浸取及膜过滤法处理烤烟样品,以D ionex C arboPac PA-1阴离子交换柱为色谱柱,0.2m o l/L N aOH水溶液为淋洗液进行分离测定。葡萄糖、果糖和蔗糖的含量与其峰面积的线性关系良好,回收率均在97%以上。方法简便易行,灵敏度高,重现性良好,可以实现对烟草中单糖的快速分离和测定。展开更多
A method for determination of iodide in complex matrix-acetic acid,glacial acetic acid and acetic anhydride was established using high performance anion exchange chromatography with pulsed amperometric detector(HPAEC-...A method for determination of iodide in complex matrix-acetic acid,glacial acetic acid and acetic anhydride was established using high performance anion exchange chromatography with pulsed amperometric detector(HPAEC-PAD).Using 200 mmol/Lnitric scid solution as mobile phase,iodide can be separated within 10 min on high hydrophilic IonPac AS16 column.The relative standard deviation(RSD)of 20μg/L iodide standard solution prepared in industrial acetic acid,glacial acetic acid and acetic anhydride was 1.8%,11.5% and 8.0%,separately.The detection limit was 0.02,0.04μg/L,separately(500μg injection,3 times of signal to moise ratio).The proposed method was applied for determination of iodide in real samples,noiodide was detected and the recovery was ranging from 85 to 111%?This method has asvantages of simplicity to prepare the samples,no inteference and high sensitivity,etc.展开更多
文摘用高效阴离子交换色谱-脉冲安培检测法(HPAEC-PAD)测定了烤烟中的水溶性葡萄糖、果糖和蔗糖。采用水浸取及膜过滤法处理烤烟样品,以D ionex C arboPac PA-1阴离子交换柱为色谱柱,0.2m o l/L N aOH水溶液为淋洗液进行分离测定。葡萄糖、果糖和蔗糖的含量与其峰面积的线性关系良好,回收率均在97%以上。方法简便易行,灵敏度高,重现性良好,可以实现对烟草中单糖的快速分离和测定。
文摘A method for determination of iodide in complex matrix-acetic acid,glacial acetic acid and acetic anhydride was established using high performance anion exchange chromatography with pulsed amperometric detector(HPAEC-PAD).Using 200 mmol/Lnitric scid solution as mobile phase,iodide can be separated within 10 min on high hydrophilic IonPac AS16 column.The relative standard deviation(RSD)of 20μg/L iodide standard solution prepared in industrial acetic acid,glacial acetic acid and acetic anhydride was 1.8%,11.5% and 8.0%,separately.The detection limit was 0.02,0.04μg/L,separately(500μg injection,3 times of signal to moise ratio).The proposed method was applied for determination of iodide in real samples,noiodide was detected and the recovery was ranging from 85 to 111%?This method has asvantages of simplicity to prepare the samples,no inteference and high sensitivity,etc.
