This study investigated the effects of progesterone(P4) on the production and survival of neurons in the hippocampal dentate gyrus of adult male mice.The administration of P4(4 mg/kg) for 3 consecutive days beginning ...This study investigated the effects of progesterone(P4) on the production and survival of neurons in the hippocampal dentate gyrus of adult male mice.The administration of P4(4 mg/kg) for 3 consecutive days beginning on the 0-2 nd day after the first BrdU-injection(BrdU-D0-2) produced an approximately twofold increase in the number of 28-and 56-day-old BrdU(+) cells in comparison to the controls,whereas it did not alter the number of 24 /48-h-old BrdU(+) cells.P4 preferentially promoted the survival of newborn neurons when administered at BrdU-D5-7,but not at BrdU-D10-12 and BrdU-D15-17.Androstenedione(Ad),testosterone(TE),or estradiol(E2) at the same-dose of P4,when administered at BrdU-D0-2,could not replicate the effect of P4,while the inhibition of 5 alpha-reductase by finasteride did not affect the P4-action,indicating that the P4-effect is exerted by P4 itself but not by its metabolites.On the other hand,the P4R antagonist RU486 partially suppressed the P4-effect,while inhibitors for Src,MEK,or PI3K totally suppressed the P4-effect.Finally,the P4-enhanced survival of newborn neurons was accompanied by a potentiation of spatial learning and memory,which was P4R-dependent.These findings suggest that P4 enhances the survival of newborn neurons through P4R and /or the Src-ERK and PI3K pathways independent of its influence on cell proliferation,which is well correlated with the potentiated spatial cognitive function of P4-treated animals.展开更多
Based on published sequences for chicken Bcl-2,three siRNAs（small interfering RNA）were designed,and expression vectors were constructed and transfected into goose granulosa cells cultured in vitro.Bcl-2 protein,apop...Based on published sequences for chicken Bcl-2,three siRNAs（small interfering RNA）were designed,and expression vectors were constructed and transfected into goose granulosa cells cultured in vitro.Bcl-2 protein,apoptosis and proliferation of granulosa cells,48 h after the transf ection,were analyzed by flow cytometry,and progesterone（P）secreted into the culture medium was measured by radioimmunoassay.In addition,apoptosis and Bcl-2 protein level were assessed in untreated granulosa cells from the four largest preovulatory follicles（F<sub>1</sub><sup>F</sup><sub>4</sub>）,the smallest preovulatory follicles（SPF）,small yellow follicles（SYF）and atretic follicles.The highest level of Bcl-2 protein was observed in granulosa cells from SPF,and levels in cells from healthy follicles were significantly higher than those of atretic follicles（P【0.05）.Bcl-2 protein levels in cells subjected to RNAi were significantly lower than those of controls（P【0.05）,while apoptosis indices（AI）,proliferation indices（PI）and P secretion in the RNAi treatments were higher than those of controls（P【0.05）.展开更多
In breeding season and non-breeding season, the effects of three kinds of progesterone plugs on estrous control in ewes were studied. Meanwhile, the advantages and the disadvantages of the progesterone plugs were anal...In breeding season and non-breeding season, the effects of three kinds of progesterone plugs on estrous control in ewes were studied. Meanwhile, the advantages and the disadvantages of the progesterone plugs were analyzed. The results showed that there were no significant difference among three progesterone plugs on modulating the ewes＇ estrus and pregnancy, and affecting estrous rate and fecundation rate （P〉0.05）. It was observed that the progesterone plug produced by Animal Husbandry Research Center of Heilongjiang Academy of Agricultural Sciences could modulate the ewes＇ estrus and pregnancy effectively, and the low cost and convenience in operation made it popularize in the reproducation of sheep widely.展开更多
磷石膏(PG)是湿法磷酸工艺中的固废产物,且堆积量较为庞大,了解磷石膏的处理方法及应用现状,可以为该领域的一线科研人员提供研发思路借鉴。运用文献计量学方法,统计了2000~2024年Web of Science(WoS)核心数据库和中国知网(CNKI)核心数...磷石膏(PG)是湿法磷酸工艺中的固废产物,且堆积量较为庞大,了解磷石膏的处理方法及应用现状,可以为该领域的一线科研人员提供研发思路借鉴。运用文献计量学方法,统计了2000~2024年Web of Science(WoS)核心数据库和中国知网(CNKI)核心数据库的相关文献进行了检索分析,深入剖析了PG应用现状、热点趋势,并对国内外各自的发展提出重点研究方向,以期为PG的资源化利用提供解决思路。结果显示:2000~2024年,国内外关于PG应用的发文量逐年上升,累计达到6408篇,PG的研究内容主要为应用过程中的杂质元素影响,其中经改性处理和掺混其他物质后,应用至建筑领域的研究愈发火热。国外初期研究注重放射性元素对环境的影响,中期注重稀土元素的提取,而国内初期研究侧重于生产化学产品,中期侧重于建筑领域应用研究,近期国内外都开始注重PG的机械强度性能测试。相较于国外PG应用行业较为集中,国内PG的应用研究注重多行业共同发展。展开更多
文摘This study investigated the effects of progesterone(P4) on the production and survival of neurons in the hippocampal dentate gyrus of adult male mice.The administration of P4(4 mg/kg) for 3 consecutive days beginning on the 0-2 nd day after the first BrdU-injection(BrdU-D0-2) produced an approximately twofold increase in the number of 28-and 56-day-old BrdU(+) cells in comparison to the controls,whereas it did not alter the number of 24 /48-h-old BrdU(+) cells.P4 preferentially promoted the survival of newborn neurons when administered at BrdU-D5-7,but not at BrdU-D10-12 and BrdU-D15-17.Androstenedione(Ad),testosterone(TE),or estradiol(E2) at the same-dose of P4,when administered at BrdU-D0-2,could not replicate the effect of P4,while the inhibition of 5 alpha-reductase by finasteride did not affect the P4-action,indicating that the P4-effect is exerted by P4 itself but not by its metabolites.On the other hand,the P4R antagonist RU486 partially suppressed the P4-effect,while inhibitors for Src,MEK,or PI3K totally suppressed the P4-effect.Finally,the P4-enhanced survival of newborn neurons was accompanied by a potentiation of spatial learning and memory,which was P4R-dependent.These findings suggest that P4 enhances the survival of newborn neurons through P4R and /or the Src-ERK and PI3K pathways independent of its influence on cell proliferation,which is well correlated with the potentiated spatial cognitive function of P4-treated animals.
基金supported by National Natural Science Foundation of China(30300253)Chen guang youth technology program of Wuhan(20065004116-25)
文摘Based on published sequences for chicken Bcl-2,three siRNAs（small interfering RNA）were designed,and expression vectors were constructed and transfected into goose granulosa cells cultured in vitro.Bcl-2 protein,apoptosis and proliferation of granulosa cells,48 h after the transf ection,were analyzed by flow cytometry,and progesterone（P）secreted into the culture medium was measured by radioimmunoassay.In addition,apoptosis and Bcl-2 protein level were assessed in untreated granulosa cells from the four largest preovulatory follicles（F<sub>1</sub><sup>F</sup><sub>4</sub>）,the smallest preovulatory follicles（SPF）,small yellow follicles（SYF）and atretic follicles.The highest level of Bcl-2 protein was observed in granulosa cells from SPF,and levels in cells from healthy follicles were significantly higher than those of atretic follicles（P【0.05）.Bcl-2 protein levels in cells subjected to RNAi were significantly lower than those of controls（P【0.05）,while apoptosis indices（AI）,proliferation indices（PI）and P secretion in the RNAi treatments were higher than those of controls（P【0.05）.
基金Supported by Heilongjiang Institute of Agricultural Sciences Youth Funded Projects
文摘In breeding season and non-breeding season, the effects of three kinds of progesterone plugs on estrous control in ewes were studied. Meanwhile, the advantages and the disadvantages of the progesterone plugs were analyzed. The results showed that there were no significant difference among three progesterone plugs on modulating the ewes＇ estrus and pregnancy, and affecting estrous rate and fecundation rate （P〉0.05）. It was observed that the progesterone plug produced by Animal Husbandry Research Center of Heilongjiang Academy of Agricultural Sciences could modulate the ewes＇ estrus and pregnancy effectively, and the low cost and convenience in operation made it popularize in the reproducation of sheep widely.
文摘磷石膏(PG)是湿法磷酸工艺中的固废产物,且堆积量较为庞大,了解磷石膏的处理方法及应用现状,可以为该领域的一线科研人员提供研发思路借鉴。运用文献计量学方法,统计了2000~2024年Web of Science(WoS)核心数据库和中国知网(CNKI)核心数据库的相关文献进行了检索分析,深入剖析了PG应用现状、热点趋势,并对国内外各自的发展提出重点研究方向,以期为PG的资源化利用提供解决思路。结果显示:2000~2024年,国内外关于PG应用的发文量逐年上升,累计达到6408篇,PG的研究内容主要为应用过程中的杂质元素影响,其中经改性处理和掺混其他物质后,应用至建筑领域的研究愈发火热。国外初期研究注重放射性元素对环境的影响,中期注重稀土元素的提取,而国内初期研究侧重于生产化学产品,中期侧重于建筑领域应用研究,近期国内外都开始注重PG的机械强度性能测试。相较于国外PG应用行业较为集中,国内PG的应用研究注重多行业共同发展。
