Profiling the protein composition of bacteria is essential for understanding their biology,physiology and interaction with environment.Mass spectrometry has become a pivotal tool for protein analysis,facilitating the ...Profiling the protein composition of bacteria is essential for understanding their biology,physiology and interaction with environment.Mass spectrometry has become a pivotal tool for protein analysis,facilitating the examination of expression levels,molecular masses and structural modifications.In this study,we compared the performance of three widely-used mass spectrometry methods,i.e.,matrix-assisted laser desorption/ionization(MALDI)protein fingerprinting,top-down proteomics and bottom-up proteomics,in the profiling of bacterial protein composition.It was revealed that bottom-up proteomics provided the highest protein coverage and exhibited the greatest protein profile overlap between bacterial species.In contrast,MALDI protein fingerprinting demonstrated superior detection reproducibility and effectiveness in distinguishing between bacterial species.Although top-down proteomics identified fewer proteins than bottom-up approach,it complemented MALDI fingerprinting in the discovery of bacterial protein markers,both favoring abundant,stable,and hydrophilic bacterial ribosomal proteins.This study represents the most systematic and comprehensive comparison of mass spectrometry-based protein profiling methodologies to date.It provides valuable guidelines for the selection of appropriate profiling strategies for specific analytical purposes.This will facilitate studies across various fields,including infection diagnosis,antimicrobial resistance detection and pharmaceutical target discovery.展开更多
Objective This study aims to analyze metabolites in the cerebrospinal fluid(CSF)of neurosyphilis patients to identify potential diagnostic markers.Methods Untargeted metabolomics by liquid chromatography-mass spectrom...Objective This study aims to analyze metabolites in the cerebrospinal fluid(CSF)of neurosyphilis patients to identify potential diagnostic markers.Methods Untargeted metabolomics by liquid chromatography-mass spectrometry was used to analyze metabolites in CSF samples from five neurosyphilis and five syphilis/non-neurosyphilis patients.After quality control and normalization,data analyses(principal component analysis,orthogonal partial least squares-discriminant analysis,hierarchical clustering)were conducted to identify differential metabolites.Results The content of metabolites(Pro-leu,nudifloramide,N-oleoyl glycine,DMP 777,(±)-propionylcarnitine,and 3β-hydroxy-5-cholenoic acid)in neurosyphilis patients was 1.7-to 4.5-fold of that in the non-neurosyphilis patients.Conclusions The metabolites,particularly nudifloramide and N-oleoyl glycine,which have been implicated in other neurological disorders,warrant further investigation into their roles in the pathogenesis of neurosyphilis.展开更多
文摘Profiling the protein composition of bacteria is essential for understanding their biology,physiology and interaction with environment.Mass spectrometry has become a pivotal tool for protein analysis,facilitating the examination of expression levels,molecular masses and structural modifications.In this study,we compared the performance of three widely-used mass spectrometry methods,i.e.,matrix-assisted laser desorption/ionization(MALDI)protein fingerprinting,top-down proteomics and bottom-up proteomics,in the profiling of bacterial protein composition.It was revealed that bottom-up proteomics provided the highest protein coverage and exhibited the greatest protein profile overlap between bacterial species.In contrast,MALDI protein fingerprinting demonstrated superior detection reproducibility and effectiveness in distinguishing between bacterial species.Although top-down proteomics identified fewer proteins than bottom-up approach,it complemented MALDI fingerprinting in the discovery of bacterial protein markers,both favoring abundant,stable,and hydrophilic bacterial ribosomal proteins.This study represents the most systematic and comprehensive comparison of mass spectrometry-based protein profiling methodologies to date.It provides valuable guidelines for the selection of appropriate profiling strategies for specific analytical purposes.This will facilitate studies across various fields,including infection diagnosis,antimicrobial resistance detection and pharmaceutical target discovery.
文摘Objective This study aims to analyze metabolites in the cerebrospinal fluid(CSF)of neurosyphilis patients to identify potential diagnostic markers.Methods Untargeted metabolomics by liquid chromatography-mass spectrometry was used to analyze metabolites in CSF samples from five neurosyphilis and five syphilis/non-neurosyphilis patients.After quality control and normalization,data analyses(principal component analysis,orthogonal partial least squares-discriminant analysis,hierarchical clustering)were conducted to identify differential metabolites.Results The content of metabolites(Pro-leu,nudifloramide,N-oleoyl glycine,DMP 777,(±)-propionylcarnitine,and 3β-hydroxy-5-cholenoic acid)in neurosyphilis patients was 1.7-to 4.5-fold of that in the non-neurosyphilis patients.Conclusions The metabolites,particularly nudifloramide and N-oleoyl glycine,which have been implicated in other neurological disorders,warrant further investigation into their roles in the pathogenesis of neurosyphilis.
