The tropomyosin(TM)fractions of crab protems may cause allergic reactions in mdividuals susceptible to allergies;however,efficient and safe methods by which to reduce such allergenicity are not currently available.The...The tropomyosin(TM)fractions of crab protems may cause allergic reactions in mdividuals susceptible to allergies;however,efficient and safe methods by which to reduce such allergenicity are not currently available.Therefore,in this study,the effects of three different processing methods,i.e.,microwave,ultrasound,and high temperature-pressure(HTP)treatments,on the digestion stability of TM from Chinese mitten crab muscle and the allergenicity of TM digestion products were explored.sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis showed that microwaving had little effect on the digestion stability of TM.In contrast,ultrasound and HTP treatments facilitated the degradation of TM.Similarly,Western blotting and inhibition ELISA indicated that the IgE-binding activity of TM was significantly reduced after treatment with ultrasound or HTP.Among the three different proces sing methods,HTP was the most effective method for improving digestibility of TM and reducing immunoreactivity.This finding provides new insights into treatments for crab allergies.展开更多
Although hazelnut oil is rich in nutrients,its quality is greatly affected by how it is processed.However,no studies to date have comprehensively analyzed the lipid composition of hazelnut oil using different processi...Although hazelnut oil is rich in nutrients,its quality is greatly affected by how it is processed.However,no studies to date have comprehensively analyzed the lipid composition of hazelnut oil using different processing methods.Here,we conducted a lipidomics analysis using UPLC-QTOF-MS to characterize the lipid composition of cold-pressed hazelnut oil(CPO),ultrasonic-assisted hexane hazelnut oil(UHO)and enzyme-assisted aqueous hazelnut oil(EAO).A total of 10 subclasses of 98 lipids were identified,including35 glycerolipids(GLs),56 glycerophospholipids(GPs)and 7 sphingolipids(SPs).The total lipid and GL content were the highest in CPO,GP content was the highest in UHO and the ceramide content in SPs was most abundant in EAO.Multivariate statistical analysis showed that the lipid profiles of hazelnut oil prepared with different processing methods varied.Twelve significantly different lipids(TAG 54:3,TAG 52:2,TAG54:4,TAG 54:2,TAG 52:3,TAG 54:5,DAG 36:2,DAG 36:4,DAG 36:3,PC 36:2,PA 36:2 and PE 36:3)were identified,and these lipids could potentially be used as biomarkers to distinguish between hazelnut oil subjected to different processing methods.Our results provide useful information for hazelnut oil applications and new insight into the effects of edible oil processing.展开更多
Alunite is the most important non bauxite resource for alumina. Various methods have been proposed and patented for processing alunite, but none has been performed at industrial scale and no technical,operational and ...Alunite is the most important non bauxite resource for alumina. Various methods have been proposed and patented for processing alunite, but none has been performed at industrial scale and no technical,operational and economic data is available to evaluate methods. In addition, selecting the right approach for alunite beneficiation, requires introducing a wide range of criteria and careful analysis of alternatives.In this research, after studying the existing processes, 13 methods were considered and evaluated by 14 technical, economic and environmental analyzing criteria. Due to multiplicity of processing methods and attributes, in this paper, Multi Attribute Decision Making methods were employed to examine the appropriateness of choices. The Delphi Analytical Hierarchy Process(DAHP) was used for weighting selection criteria and Fuzzy TOPSIS approach was used to determine the most profitable candidates. Among 13 studied methods, Spanish, Svoronos and Hazan methods were respectively recognized to be the best choices.展开更多
The spectrum user’s librarys containing 1533 familiar toxicants have been built; A method with high separability and sensitivity has been found,The RT and the detection limit of 210 familiar abuse drugs has been conf...The spectrum user’s librarys containing 1533 familiar toxicants have been built; A method with high separability and sensitivity has been found,The RT and the detection limit of 210 familiar abuse drugs has been confirmed; the processing methods to analyse spectrum data automatically have been found; It is simple,quick,sensitive and reliable,and can take place of manual operation,It is very useful to screen the familiar toxicants in unkown sample.展开更多
The screening of particles with different vibration modes was simulated by means of a 3D discrete element method (3D-DEM). The motion and penetration of the particles on the screen deck were analyzed for linear, circu...The screening of particles with different vibration modes was simulated by means of a 3D discrete element method (3D-DEM). The motion and penetration of the particles on the screen deck were analyzed for linear, circular and elliptical vibration of the screen. The results show that the travel velocity of the particles is the fastest, but the screening efficiency is the lowest, for the linear vibration mode. The circular motion resulted in the highest screening efficiency, but the lowest particle travel velocity. In the steady state the screening efficiency for each mode is seen to increase gradually along the longitudinal direction of the deck. The screening efficiency increment of the circular mode is the largest while the linear mode shows the smallest increment. The volume fraction of near-mesh size particles at the underside is larger than that of small size particles all along the screen deck. Linear screening mode has more near-mesh and small size particles on the first three deck sections, and fewer on the last two sections, compared to the circular or elliptical modes.展开更多
The structure of a microlens array（ MLA） can be formed on copper by an indentation process which is a new manufacture approach we applied here instead of a traditional method to test the material property,thereby wo...The structure of a microlens array（ MLA） can be formed on copper by an indentation process which is a new manufacture approach we applied here instead of a traditional method to test the material property,thereby work time can be saved. Single-indentation and multi-indentation are both conducted to generate a single dimple and dimples array,namely micro lens and MLA. Based on finite element simulation method,factors affecting the form accuracy,such as springback at the compressed area of one single dimple and compressional deformation at the adjacent area of dimples arrays,are determined,and the results are verified by experiments under the same conditions. Meanwhile,indenter compensation method is proposed to improve form accuracy of single dimple,and the relationship between pitch and compressional deformation is investigated by modelling seven sets of multi-indentations at different pitches to identify the critical pitch for the MLA＇s indentation processing. Loads and cross-sectional profiles are measured and analyzed to reveal the compressional deformation mechanism. Finally,it is found that MLA at pitches higher than 1. 47 times of its diameter can be manufactured precisely by indentation using a compensated indenter.展开更多
In order to study the failure process of an anchorage structure and the evolution law of the body＇s defor- mation field, anchor push-out tests were carried out based on digital speckle correlation methods （DSCM）. T...In order to study the failure process of an anchorage structure and the evolution law of the body＇s defor- mation field, anchor push-out tests were carried out based on digital speckle correlation methods （DSCM）. The stress distribution of the anchorage interface was investigated using the particle flow numerical simulation method. The results indicate that there are three stages in the deformation and fail- ure process of an anchorage structure： elastic bonding stage, a de-bonding stage and a failure stage. The stress distribution in the interface controls the stability of the structure. In the elastic bonding stage, the shear stress peak point of the interface is close to the loading end, and the displacement field gradually develops into a ＂V＂ shape, in the de-bonding stage, there is a shear stress plateau in the center of the anchorage section, and shear strain localization begins to form in the deformation field. In the failure stage, the bonding of the interface fails rapidly and the shear stress peak point moves to the anchorage free end. The anchorage structure moves integrally along the macro-cracl~ The de-bonding stage is a research focus in the deformation and failure process of an anchorage structure, and plays an important guiding role in roadway support design and prediction of the stability of the surrounding rock.展开更多
Proper matching of forestry machinery is important when raising mechanization levels for forestry production. In the matching process, forestry machinery needs not only expertise, but also improved methods for solving...Proper matching of forestry machinery is important when raising mechanization levels for forestry production. In the matching process, forestry machinery needs not only expertise, but also improved methods for solving problems. I propose combination of case-based reasoning （CBR） and rule-based reasoning （RBR） by calculating the similarity of quantitative parameters of various forestry machines in an analytical and hierarchical process. I calculated the similarity of machin-ery used in forest industries to enable better selection and matching of equipment. I propose a weight-value adjusting method based on sums of squares of deviations in which the individual parameter weights were modified in the process of application. During the process of system design, I put forward a design method knowledge base and generated a dynamic web reasoning framework to integrate the processes of forest industry machinery selection and weight-value adjustment. This enables expansion of the scope of the complete system and enhancement of the reasoning efficiency. I demonstrate the validity and practicability of this method using a practical example.展开更多
Life science has a need for detection methods that are label-free and real-time. In this paper, we have selected staphylococcal protein A （SPA） and swine immunoglobulin G （IgG）, and monitor the bindings between SP...Life science has a need for detection methods that are label-free and real-time. In this paper, we have selected staphylococcal protein A （SPA） and swine immunoglobulin G （IgG）, and monitor the bindings between SPA and swine IgG with different concentrations, as well as the dissociations of SPA-swine IgG complex in different pH values of phosphate buffer by oblique-incidence reflectivity difference （OIRD） in a label-free and real-time fashion. We obtain the ON and OFF reaction dynamic curves corresponding to the bindings and dissociations of SPA and swine IgG. Through our analysis of the experimental results, we have been able to obtain the damping coefficients and the dissociation time of SPA and swine IgG for different pH values of the phosphate buffer. The results prove that the OIRD technique is a competing method for monitoring the dynamic processes of biomolecule interaction and achieving the quantitative information of reaction kinetics.展开更多
基金supported by the earmarked fund for the Anhui Provincial Modern Agri-industry Technology Research System(AHCYJSTX-08)the China Agriculture Research System of MOF and MARA(CARS-48)。
文摘The tropomyosin(TM)fractions of crab protems may cause allergic reactions in mdividuals susceptible to allergies;however,efficient and safe methods by which to reduce such allergenicity are not currently available.Therefore,in this study,the effects of three different processing methods,i.e.,microwave,ultrasound,and high temperature-pressure(HTP)treatments,on the digestion stability of TM from Chinese mitten crab muscle and the allergenicity of TM digestion products were explored.sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis showed that microwaving had little effect on the digestion stability of TM.In contrast,ultrasound and HTP treatments facilitated the degradation of TM.Similarly,Western blotting and inhibition ELISA indicated that the IgE-binding activity of TM was significantly reduced after treatment with ultrasound or HTP.Among the three different proces sing methods,HTP was the most effective method for improving digestibility of TM and reducing immunoreactivity.This finding provides new insights into treatments for crab allergies.
基金supported by Key R&D Project of Liaoning Province,under Grant Research and Demonstration of Key Technologies for Deep Processing and Comprehensive Utilization of Northeast Hazelnuts(2020JH2/10200037)Service Local Project of Liaoning Province,under Grant Demonstration and Promotion of new deep-processing technology for comprehensive utilization of Northeast Hazelnuts(LSNFW201903)horizontal subject,under Grant Demonstration and Promotion of key technologies for transformation and deep processing of wild hazelnut forest in northwestern Liaoning(H2019388)。
文摘Although hazelnut oil is rich in nutrients,its quality is greatly affected by how it is processed.However,no studies to date have comprehensively analyzed the lipid composition of hazelnut oil using different processing methods.Here,we conducted a lipidomics analysis using UPLC-QTOF-MS to characterize the lipid composition of cold-pressed hazelnut oil(CPO),ultrasonic-assisted hexane hazelnut oil(UHO)and enzyme-assisted aqueous hazelnut oil(EAO).A total of 10 subclasses of 98 lipids were identified,including35 glycerolipids(GLs),56 glycerophospholipids(GPs)and 7 sphingolipids(SPs).The total lipid and GL content were the highest in CPO,GP content was the highest in UHO and the ceramide content in SPs was most abundant in EAO.Multivariate statistical analysis showed that the lipid profiles of hazelnut oil prepared with different processing methods varied.Twelve significantly different lipids(TAG 54:3,TAG 52:2,TAG54:4,TAG 54:2,TAG 52:3,TAG 54:5,DAG 36:2,DAG 36:4,DAG 36:3,PC 36:2,PA 36:2 and PE 36:3)were identified,and these lipids could potentially be used as biomarkers to distinguish between hazelnut oil subjected to different processing methods.Our results provide useful information for hazelnut oil applications and new insight into the effects of edible oil processing.
文摘Alunite is the most important non bauxite resource for alumina. Various methods have been proposed and patented for processing alunite, but none has been performed at industrial scale and no technical,operational and economic data is available to evaluate methods. In addition, selecting the right approach for alunite beneficiation, requires introducing a wide range of criteria and careful analysis of alternatives.In this research, after studying the existing processes, 13 methods were considered and evaluated by 14 technical, economic and environmental analyzing criteria. Due to multiplicity of processing methods and attributes, in this paper, Multi Attribute Decision Making methods were employed to examine the appropriateness of choices. The Delphi Analytical Hierarchy Process(DAHP) was used for weighting selection criteria and Fuzzy TOPSIS approach was used to determine the most profitable candidates. Among 13 studied methods, Spanish, Svoronos and Hazan methods were respectively recognized to be the best choices.
文摘The spectrum user’s librarys containing 1533 familiar toxicants have been built; A method with high separability and sensitivity has been found,The RT and the detection limit of 210 familiar abuse drugs has been confirmed; the processing methods to analyse spectrum data automatically have been found; It is simple,quick,sensitive and reliable,and can take place of manual operation,It is very useful to screen the familiar toxicants in unkown sample.
基金financial support from the National Natural Science Foundation of China (No. 51204181)the Research Fund for the Doctoral Program of Higher Education of China (No.20110095120004)+1 种基金the Fundamental Research Funds for the Central Universities (Nos. 2011QNA10 and 2010QNB17)the China Postdoctoral Science Foundation (No. 20110491485) for this work
文摘The screening of particles with different vibration modes was simulated by means of a 3D discrete element method (3D-DEM). The motion and penetration of the particles on the screen deck were analyzed for linear, circular and elliptical vibration of the screen. The results show that the travel velocity of the particles is the fastest, but the screening efficiency is the lowest, for the linear vibration mode. The circular motion resulted in the highest screening efficiency, but the lowest particle travel velocity. In the steady state the screening efficiency for each mode is seen to increase gradually along the longitudinal direction of the deck. The screening efficiency increment of the circular mode is the largest while the linear mode shows the smallest increment. The volume fraction of near-mesh size particles at the underside is larger than that of small size particles all along the screen deck. Linear screening mode has more near-mesh and small size particles on the first three deck sections, and fewer on the last two sections, compared to the circular or elliptical modes.
基金Supported by the National Natural Science Foundation of China(51375050)
文摘The structure of a microlens array（ MLA） can be formed on copper by an indentation process which is a new manufacture approach we applied here instead of a traditional method to test the material property,thereby work time can be saved. Single-indentation and multi-indentation are both conducted to generate a single dimple and dimples array,namely micro lens and MLA. Based on finite element simulation method,factors affecting the form accuracy,such as springback at the compressed area of one single dimple and compressional deformation at the adjacent area of dimples arrays,are determined,and the results are verified by experiments under the same conditions. Meanwhile,indenter compensation method is proposed to improve form accuracy of single dimple,and the relationship between pitch and compressional deformation is investigated by modelling seven sets of multi-indentations at different pitches to identify the critical pitch for the MLA＇s indentation processing. Loads and cross-sectional profiles are measured and analyzed to reveal the compressional deformation mechanism. Finally,it is found that MLA at pitches higher than 1. 47 times of its diameter can be manufactured precisely by indentation using a compensated indenter.
基金financially supported by the National Key Basic Research Program of China (No.2010CB226805)the National Natural Science Foundation of China (Nos.51474136 and 51474013)+1 种基金the Opening Project Fund of State Key Laboratory of Mining Disaster Prevention and Control Co-founded by Shandong Province and the Ministry of Science and Technology (No.MDPC2013KF06)the Research Award Fund for the Excellent Youth of Shandong University of Science and Technology (No.2011KYJQ106)
文摘In order to study the failure process of an anchorage structure and the evolution law of the body＇s defor- mation field, anchor push-out tests were carried out based on digital speckle correlation methods （DSCM）. The stress distribution of the anchorage interface was investigated using the particle flow numerical simulation method. The results indicate that there are three stages in the deformation and fail- ure process of an anchorage structure： elastic bonding stage, a de-bonding stage and a failure stage. The stress distribution in the interface controls the stability of the structure. In the elastic bonding stage, the shear stress peak point of the interface is close to the loading end, and the displacement field gradually develops into a ＂V＂ shape, in the de-bonding stage, there is a shear stress plateau in the center of the anchorage section, and shear strain localization begins to form in the deformation field. In the failure stage, the bonding of the interface fails rapidly and the shear stress peak point moves to the anchorage free end. The anchorage structure moves integrally along the macro-cracl~ The de-bonding stage is a research focus in the deformation and failure process of an anchorage structure, and plays an important guiding role in roadway support design and prediction of the stability of the surrounding rock.
基金financially supported by the Fundamental Research Funds for the Central Universities Nos.DL12EB01-03the planning subject of "the Twelfth Five-Year-Plan" in National Science and Technology Nos.2012AA102003-2Heilongjiang Natural Science Fund in China Nos.F201116
文摘Proper matching of forestry machinery is important when raising mechanization levels for forestry production. In the matching process, forestry machinery needs not only expertise, but also improved methods for solving problems. I propose combination of case-based reasoning （CBR） and rule-based reasoning （RBR） by calculating the similarity of quantitative parameters of various forestry machines in an analytical and hierarchical process. I calculated the similarity of machin-ery used in forest industries to enable better selection and matching of equipment. I propose a weight-value adjusting method based on sums of squares of deviations in which the individual parameter weights were modified in the process of application. During the process of system design, I put forward a design method knowledge base and generated a dynamic web reasoning framework to integrate the processes of forest industry machinery selection and weight-value adjustment. This enables expansion of the scope of the complete system and enhancement of the reasoning efficiency. I demonstrate the validity and practicability of this method using a practical example.
基金Supported by the Key Research Program of Chinese Academy of Sciences
文摘Life science has a need for detection methods that are label-free and real-time. In this paper, we have selected staphylococcal protein A （SPA） and swine immunoglobulin G （IgG）, and monitor the bindings between SPA and swine IgG with different concentrations, as well as the dissociations of SPA-swine IgG complex in different pH values of phosphate buffer by oblique-incidence reflectivity difference （OIRD） in a label-free and real-time fashion. We obtain the ON and OFF reaction dynamic curves corresponding to the bindings and dissociations of SPA and swine IgG. Through our analysis of the experimental results, we have been able to obtain the damping coefficients and the dissociation time of SPA and swine IgG for different pH values of the phosphate buffer. The results prove that the OIRD technique is a competing method for monitoring the dynamic processes of biomolecule interaction and achieving the quantitative information of reaction kinetics.
