Well-crystallized hexagonal hematite （α-Fe2O3） platelets were synthesized by hydrothermal process, using a highly concentrated ferric hydroxide as precursor. The precursor was prepared by adding ammonia to the ferr...Well-crystallized hexagonal hematite （α-Fe2O3） platelets were synthesized by hydrothermal process, using a highly concentrated ferric hydroxide as precursor. The precursor was prepared by adding ammonia to the ferric sulfate solution which was obtained by leaching pyrite cinders with sulfuric acid. Structure and morphology of the synthesized products were investigated by X-ray diffraction, scanning electron microscope, transmission electron microscope and selected area electron diffraction. The results reveal that the reaction temperature has significant effects on the structure, size and shape of the synthesized hematite particles. Typical hexagonal hematite platelets, about 0.4-0.6 μm in diameter and 0.1 μm in thickness, were prepared at 230 ℃ for 0.5 h. Al^3＋, contained in the sulfuric acid leaching solution as an impurity, plays an extremely important role in the formation of hexagonal hematite. In addition, a possible mechanism about the formation of hexagonal hematite platelets was proposed.展开更多
The nonlinear vibration of graphene platelets reinforced composite corrugated(GPRCC)rectangular plates with shallow trapezoidal corrugations is investigated.Since graphene platelets are prone to agglomeration,a multi-...The nonlinear vibration of graphene platelets reinforced composite corrugated(GPRCC)rectangular plates with shallow trapezoidal corrugations is investigated.Since graphene platelets are prone to agglomeration,a multi-layer distribution is adopted here to match the engineering requirements.Firstly,an equivalent composite plate model is obtained,and then nonlinear equations of motion are derived by the von Kármán nonlinear geometric relationship and Hamilton’s principle.Afterwards,the Galerkin method and harmonic balance method are used to obtain an approximate analytical solution.Results show that the unit cell half period,unit cell inclination angle,unit cell height,graphene platelet dispersion pattern and graphene platelet weight fraction and geometry play important roles in the nonlinear vibration of the GPRCC plates.展开更多
The bending and stress analysis of a functionally graded polymer composite plate reinforced with graphene platelets are studied in this paper.The governing equations are derived by using principle of virtual work for ...The bending and stress analysis of a functionally graded polymer composite plate reinforced with graphene platelets are studied in this paper.The governing equations are derived by using principle of virtual work for a plate which is rested on Pasternak’s foundation.Sinusoidal shear deformation theory is used to describe displacement field.Four different distribution patterns are employed in our analysis.The analytical solution is presented for a functionally graded plate to investigate the influence of important parameters.The numerical results are presented to show the deflection and stress results of the problem for four employed patterns in terms of geometric parameters such as number of layers,weight fraction and two parameters of Pasternak’s foundation.展开更多
McEver和Berilacqua等将一组结构、功能相似的具有植物血凝素样结构的超家族称为选择素,它属于粘附分子。血小板上的选择素超家族便是血小板α颗粒膜蛋白140(α-granule membrane protein 140,GMP—140)。它存在于血小板的α颗粒膜上,...McEver和Berilacqua等将一组结构、功能相似的具有植物血凝素样结构的超家族称为选择素,它属于粘附分子。血小板上的选择素超家族便是血小板α颗粒膜蛋白140(α-granule membrane protein 140,GMP—140)。它存在于血小板的α颗粒膜上,只是在血小板被激活时才在血小板表面表达,因此它成为反映血小板活化的特异性指标。随着单克隆抗体及分子生物学技术等的应用,对GMP—140的特性、结构等已有一定的认识。在临床方面,抗人活化血小板表面GMP—140单克隆抗体的应用,一方面可直接判断体内血小板的活化程度。展开更多
Objective To develop a plasma pharmacological method evaluates the effect of Da Huang Zhe Chong capsule on platelet aggregation and its mechanism, which is a representative Traditional Chinese Medicine Patent Prescrip...Objective To develop a plasma pharmacological method evaluates the effect of Da Huang Zhe Chong capsule on platelet aggregation and its mechanism, which is a representative Traditional Chinese Medicine Patent Prescription Promoting blood circulation by removing blood stasis. Methods Platelets specimens from healthy volunteers made serum and plasma with medicine, while platelet PRP were separated, which were divided into 8groups,i.e. auto-serum, allo-serum, serum with Da Huang Zhe Chong capsule , serum with aspirin, auto-plasma, plasma with Da Huang Zhe Chong capsule, plasma with aspirin, every group added to serum and plasma to hatch. After ADP and adrenalin were added into the specimens and hatched, the effects of specimens on platelet aggregation were observed. Results After ADP adrenalin were added, all the serum groups did not present platelet aggregation,while all the plasma group presented platelet aggregation. P1, P5, Pmax, t and TM have no significant difference (P＞0. 05) between auto-plasma group and allo-plasma group induced by ADP and adrenalin. P1, P5, t and Pmax have significant differences (P＜0. 01) and TM decreased significantly (P＜0. 05) comparing plasma group with Da Huang Zhe Chong capsule and plasma group of aspirin to allo-plasma group. P1, t and Pmax have significant difference (P＜0.05), and P5 and TM are simulate comparing plasma group with Da Huang Zhe Chong capsule to plasma group of aspirin. P1, P5, t and Pmax have significant differences (P＜0. 01), P1, TM have also significant(P＜0. 05), comparing plasma group of Da Huang Zhe Chong capsule with plasma of aspirin to allo-plasma group induced by adrenalin. P1 ,P5 and Pmax have significant differences (P＜0.05), and t and Pmax are simulate comparing plasma group with Da Huang Zhe Chong capsule with plasma group of aspirin. Conclusion The serum pharmacological study is inappropriate to study platelet aggregation in vitro. The plasma pharmacological study is inappropriate to study platelet aggregation in vitro. The plasma pharmacological study could reflect the pharmacological effect produced in vivo. Da Huang Zhe Chong capsule has better anti artery thrombosis effect than aspirin, and it is an ideal medicine for anti artery thrombosis.展开更多
OBJECTIVE Salvia miltiorrhiza bunge contains various active constituents,some of which have been developed as commercially available medicine.Moreover,some other ingredients in Salvia miltiorrhiza play great roles in ...OBJECTIVE Salvia miltiorrhiza bunge contains various active constituents,some of which have been developed as commercially available medicine.Moreover,some other ingredients in Salvia miltiorrhiza play great roles in anti-platelet activity.The aim of the present study was to investigate the effects and the underlying mechanism of miltirone,a lipophilic compound of Salvia miltiorrhiza Bunge.METHODS The ability of miltirone to modulate platelet function was investigated by a variety of in vitro and in vivo experiments.Platelet aggregation and dense granule secretion induced by various agonists were measured with platelet aggregometer.Clot retraction and spreading were imaged by digital camera and fl uorescence microscope.Ferric chloride-induced carotid injury model and pulmonary thromboembolism model were used to check miltirone effect in vivo.To elucidate the mechanisms of anti-platelet activity of miltirone,flow cytometry and Western blotting were performed.RESULTS Miltirone(2,4,8 μmol·L^(-1)) was shown to suppress platelet aggregation,dense granule and α granule secretion in a dose-dependent manner.Meanwhile,miltirone inhibited the clot retraction and spreading of washed platelets.It reduced the phosphorylation of PLCγ2,PKC,Akt,GSK3β and ERK1/2 in the downstream signal pathway of collagen receptor.It also reduced the phosphorylation of Src and FAK in the integrin αⅡbβ3 mediated "outside-in" signaling,while it did not suppress the phosphorylation of β3.In addition,miltirone prolonged the occlusion time and reduced collagen/epinephrine induced pulmonary thrombi.CONCLUSION Miltirone suppresses platelet "inside-out" and "outside-in" signaling by affecting PLCγ2/PKC/ERK1/2,PI3K/Akt and Src/FAK signaling.Therefore,miltirone might represent a potential anti-platelet candidate for the prevention of thrombotic disorders.展开更多
Abstract：Aim To investigate the effect of hydrogen-rich saline on arterial thrombosis in sinoaortic denervatted （SAD） rats. Methods Rats were divided randomly into Sham group, SAD group, SAD ＋ hydrogen-rich saline...Abstract：Aim To investigate the effect of hydrogen-rich saline on arterial thrombosis in sinoaortic denervatted （SAD） rats. Methods Rats were divided randomly into Sham group, SAD group, SAD ＋ hydrogen-rich saline （5 mL · kg^-1 · d^-1） group and SAD ＋ hydrogen-rich saline （10 mL · kg^-1 · d^-1） group. Four weeks after Sham or SAD operation, rats were given intraperitoneally normal saline or hydrogen-rich saline for 4 weeks. Carotid artery thrombosis was induced by 30% ferric chloride. Time till occlusion was measured by Laser Doppler Flowme- try. Results When compared with the Sham group, time till occlusion was significantly shorter in SAD group. Moreover, platelet aggregation, platelet adhesion to collagen and the level of ROS and malondialdehyde （MDA） in plasma and Ca2＋ in platelets were significantly raised. The level of NO and SOD in plasma and NO in platelet were significantly reduced. When compared with the SAD group, time till occlusion in hydrogen-rich saline group group was significantly prolonged. Platelet aggregation, adhesion, the level of ROS, MDA and Ca2＋ were significantly re- duced, whereas the level of NO and SOD in plasma and NO in platelets were significantly higher. Conclusion Hydrogen-rich saline might improve arterial thrombosis in SAD rats through inhibition of platelet activation and oxi- dative stress.展开更多
Platelets aggregation and thrombosis formation are major reasons of cardiovascular and cerebral vascular diseases.To develop new generative,potent and safe agents for inhibiting platelet aggregation and preventing abo...Platelets aggregation and thrombosis formation are major reasons of cardiovascular and cerebral vascular diseases.To develop new generative,potent and safe agents for inhibiting platelet aggregation and preventing above diseases are urgently required.Some traditional Chinese medicines of″Houxue Huayu″have been shown to inhibit platelet aggregation potently.In the present study the mechanisms and the molecular targets of puerarin,salvianolic acid B and the analogue of 3-n-butylphthalide,dl-PHPB were investigated and compared with ticlopidine.Four platelet aggregation inducers,ADP,arachidonic acid,collagen and thrombin were used in the study.It was found that puerarin and dl-PHPB specifically inhibited ADP induced platelet aggregation like ticlopidine did.However,salvianolic acid B inhibited both ADP and collagen induced platelet aggregations with similar potency.Due to existing two ADP receptor subtypes on platelets,P2Y1 and P2Y12,we studied the action of above compounds on the receptors and the signaling pathways.It was found that dl-PHPB decreased IP1 accumulation produced by ADP,but had no effect on IP1 level induced by m-3M3 FBS,an activator of PLC.M-3M3 FBS might attenuate the inhibitory effect of dl-PHPB on ADP-induced platelet aggregation.In addition,dl-PHPB did not affect cyclic AMP formation in platelets by ADP,which is different from P2Y12 antagonist ticlopidine.Puerarin showed the similar effects of dl-PHPB.Therefore,the actions of dl-PHPB and puerarin might be through P2Y1receptor-PLC-βpathway.Salvianolic acid B did not reduce the IP1 accumulation stimulated by ADP.It might act on the receptor subtype P2Y12.Our results suggest that components of Chinese herb medicine might be a resource for development of novel anti-platelet drugs.展开更多
Aim To evaluate the time-effect and dose-effect of prasugrel hydrobromide acetic acid compound （PHAAC） inhibiting platelet aggregation. Methods For the time-effect study, 190 Sprague-Dawley （SD） rats were devided ...Aim To evaluate the time-effect and dose-effect of prasugrel hydrobromide acetic acid compound （PHAAC） inhibiting platelet aggregation. Methods For the time-effect study, 190 Sprague-Dawley （SD） rats were devided into 19 groups （n- 10）： the vehicle control group, the PHAAC groups （0.5, 1, 2, 4, 6, 24, 48, 72, 96 h） and the prasugrel hydrochloride groups （0.5, 1, 2, 4, 6, 24, 48, 72, 96 h）. Rats were singly intra- gastic administration of the vehicle, the PHAAC （5 mg·kg^-1） or the prasugrel hydrochloride （5 mg · kg^-1 ）, re- spectively. Blood samples were taken at each time point for the determination of platelet aggregation rate （PAR）. For the dose-effect study, 110 SD rats were devided into 11 groups （n= 10）： the vehicle control group, the PHAAC groups （10, 5, 2.5, 1, 0.5 mg · kg^-1, dosage of prasugrel） and the prasugrel hydrochloride groups （ 10, 5, 2.5, 1, 0.5 mg · kg^-1, dosage of prasugrel） . Blood samples were taken at 4 h after drug administration for the determination of PAR. Results Compared with the vehicle group, PHAAC has significant anti-platelet ag- gregative effects （P 〈 0.05） at the time of 0.5, 1, 2, 4, 6, 24, 48 h, and the effect at the time of 4 h was the strongest. There were no obvious differences between the effect of PHAAC （5 mg · kg^-1） and prasugrel hydrochlo- ride （5 mg · kg^-1） at each time point. Compared with the vehicle group, intragastic administration of PHAAC at the doses of 10, 5, 2.5, 1, 0.5 mg · kg^-1 could obviously inhibite the platelet aggregation, and showed a dose- dependent manner. There were no significant differences between the effect of PHAAC and prasugrel hydrochloride at the same dose. Conclusion PHAAC can inhibit platelet aggregation in a dose-dependent manner, and the effect at 4 h after drug administration is the strongest. The action strength and duration of PHAAC are similar with that of the prasugrel hydrochloride.展开更多
OBJECTIVE To investigate the effect of hydrogen-rich saline on platelet activation in hypertensive rats.METHODS The male Wistar rats were divided into control group,hypertension group,control+hydrogen-rich saline grou...OBJECTIVE To investigate the effect of hydrogen-rich saline on platelet activation in hypertensive rats.METHODS The male Wistar rats were divided into control group,hypertension group,control+hydrogen-rich saline group and hypertension+hydrogen-rich saline group.Hypertension was induced by subcutaneous infusion with angiotensinⅡ0.7 mg·kg-1·d-1for 2 weeks by osmotic mini-pumps in rats.Hydrogen-rich saline(10 mL·kg-1·d-1)was administered by intraperitoneal injection for 14 d.Platelet adhesion on collagen surface was evaluated using a well-defined perfusion chamber at low shear rate(300s-1)and high shear rate(1080s-1).The maximum aggregation rate of platelets induced by ADP was determined by turbidimetry.The levels of reactive oxygen species(ROS),nitric oxide(NO)and Ca2+in platelets were measured with flow cytometry.RESULTS When compared with the control group,platelet aggregation,platelet adhesion rate in high shear rate and low shear rate,and the level of ROS and Ca2+in platelets were elevated in hypertensive group.However,NO level in platelets decreased.Compared with the hypertensive group,hydrogen-rich saline treatment decreased platelet aggregation,platelet adhesion rate,the levels of ROS and Ca2+in platelets,and increased NO level in platelets of hypertensive rats.CONCLUSION Hydrogen-rich saline could inhibit platelet activation in hypertensive rats.This effect may be related to antioxidative stress.展开更多
Fetal liver tissues obtained from 28 human fetuses with gestation age from 3 to 6 months and fetal bone marrow from 35 human fetuses from 3 to 7 months were observed by immunochemical staining with anti-platelet GPⅡ ...Fetal liver tissues obtained from 28 human fetuses with gestation age from 3 to 6 months and fetal bone marrow from 35 human fetuses from 3 to 7 months were observed by immunochemical staining with anti-platelet GPⅡ b / Ⅲa monoclonal antibody and ABC technique. In the fetal liver, megakaryocytes were wholly located among growing fetal liver cells and near foci of hemopoiesis. Some megakaryocytes in the fetal liver were small7890- lymphoid-like megakaryocytes. The size of megakaryocytes both in the fetal liver (14.79 ± 4.52μm) and in the fetal bone marrow (16.08±7.39 μm) was small, which did not vary significantly over the gestation age ranging from 3 to 6 or 7 months. However, the maturation stage of megakaryocytes in the fetal liver shifted to more mature stage with the advancement of gestation, although the maturation stage of megakaryocytes in the fetal bone marrow did not change with the advancement of gestation from 4 to 7 months, the megakaryocyte in the fetal bone marrow was less mature展开更多
Background and Objective Renal insufficiency(RI)is reported to be associated with increased ischemic and bleeding events after percutaneous coronary intervention(PCI),which is possibly due to high residual platelet re...Background and Objective Renal insufficiency(RI)is reported to be associated with increased ischemic and bleeding events after percutaneous coronary intervention(PCI),which is possibly due to high residual platelet reactivity(HRPR)during DAPT therapy.Therefore,we performed a large prospective observational study to evaluate the platelet reactivity and related clinical outcomes in real-world patients with different renal insufficiency stage after PCI,and to examine whether HRPR is associated with higher incidence of adverse cardiovascular events in a 2-year follow up.展开更多
OBJECTIVE Platelets play a major role in mediating inflammatory response.The present work was designed to investigate whether arterial baroreflex impairment induced by sinoaortic denervation(SAD)affect platelet activa...OBJECTIVE Platelets play a major role in mediating inflammatory response.The present work was designed to investigate whether arterial baroreflex impairment induced by sinoaortic denervation(SAD)affect platelet activation,leading to the exacerbation of cerebral cortex and hippocampus inflammation in rats.METHODS Adult male SD rats were divided into four groups:the sham control,the sinoaortic denervation(SAD),the sham+LPS,the SAD+LPS.In another experiment,there were also four groups:the sham control,the SAD,the SAD+LPS and the SAD+LPS+asprin.Four weeks after sham or SAD surgery,all rats were examined for the level of CD41,CD45,IL-1βand PF-4 in the cerebral cortex and hippocampus using immunofluorescence and ELISA.Blood platelet and leukocyte count,platelet microaggre⁃gation,expression of CD154 and CD62P on platelet surface and platelet-leukocyte aggregate level was detected by flow cytometry.RESULTS Compared with sham+LPS group,the in SAD+LPS group rats exhibited the high level of CD41,CD45,IL-1βand PF-4 in the cerebral cortex and hippocampus.Leukocyte count,platelet microag⁃gregation,expression of CD154 and CD62P on platelet surface and platelet-leukocyte aggregate level was increased,while blood platelet count was decreased in the SAD+LPS.Moreover,all the above changes were improved in the SAD+LPS+asprin group when compared with the SAD+LPS group.CONCLUSION Arterial baroreflex dysfunction exacerbates inflammation in the rat cerebral cortex and hippocampus,which is likely mediated by platelet.展开更多
OBJECTIVE To screen the active ingredients preventing platelet aggregations from Taohong Siwu Decoction. METHODS Fresh blood from healthy adult volunteer was collected with vacuum tube for preparation of platelet susp...OBJECTIVE To screen the active ingredients preventing platelet aggregations from Taohong Siwu Decoction. METHODS Fresh blood from healthy adult volunteer was collected with vacuum tube for preparation of platelet suspension. Platelet suspension was incubated with Taohong Siwu Decoction for one hour at 37℃,then centrifuged. The precipitate was washed and desorbed with buffer solutions.The differences of chemical constituents between incubated and unincubated were identified with high performance liquid chromatography-diode array detector. RESULTS According to the chromatograph at 254 nm,at least three kinds of different components were detected in Taohong siwu decoction before and after incubated with platelet. CONCLUSION Platelet suspension can be used for screening active ingredients on platelet membrane receptor from the traditional Chinese medicine.展开更多
Background and Objective The white blood cell count to mean platelet volume ratio(WMR)has recently been described as a predictor of cardiovascular events in patients who undergo percutaneous coronary intervention(PCI)...Background and Objective The white blood cell count to mean platelet volume ratio(WMR)has recently been described as a predictor of cardiovascular events in patients who undergo percutaneous coronary intervention(PCI).The aim of this study was to investigate the usefulness of admission WMR in predicting outcomes in patients with acute coronary syndrome(ACS).展开更多
Arsenic in drinking water is a worldwide health problem that is associated with cardiovascular disease, but the cause is currently unknown. In order to examine whether arsenic affects vasomotor tone in blood vessels, ...Arsenic in drinking water is a worldwide health problem that is associated with cardiovascular disease, but the cause is currently unknown. In order to examine whether arsenic affects vasomotor tone in blood vessels, we investigated the effect of arsenic on agonist-induced vasorelaxation and vasoconstriction using the isolated rat aortic rings in in vitro organ bath system. Treatment with inorganic arsenite (AsⅢ) inhibited acetylcholine-induced relaxation of aortic rings by inhibiting production of nitric oxide in endothelium.展开更多
The size mismatch in an end-to-end vascular anastomosis between the host vessel and the graft may cause flow disturbance and predispose to thrombosis [1].Although a number of techniques have been employed to reduce th...The size mismatch in an end-to-end vascular anastomosis between the host vessel and the graft may cause flow disturbance and predispose to thrombosis [1].Although a number of techniques have been employed to reduce the risk of anastomotic thrombosis due to the size mismatch。展开更多
基金Project(2008A090300016) supported by the Key Science and Technology Item of Guangdong Province,ChinaProject(ZKJ2010022) supported by the Precious Apparatus Opening Center Foundation of Central South University,China
文摘Well-crystallized hexagonal hematite （α-Fe2O3） platelets were synthesized by hydrothermal process, using a highly concentrated ferric hydroxide as precursor. The precursor was prepared by adding ammonia to the ferric sulfate solution which was obtained by leaching pyrite cinders with sulfuric acid. Structure and morphology of the synthesized products were investigated by X-ray diffraction, scanning electron microscope, transmission electron microscope and selected area electron diffraction. The results reveal that the reaction temperature has significant effects on the structure, size and shape of the synthesized hematite particles. Typical hexagonal hematite platelets, about 0.4-0.6 μm in diameter and 0.1 μm in thickness, were prepared at 230 ℃ for 0.5 h. Al^3＋, contained in the sulfuric acid leaching solution as an impurity, plays an extremely important role in the formation of hexagonal hematite. In addition, a possible mechanism about the formation of hexagonal hematite platelets was proposed.
基金Project(11972204) supported by the National Natural Science Foundation of China。
文摘The nonlinear vibration of graphene platelets reinforced composite corrugated(GPRCC)rectangular plates with shallow trapezoidal corrugations is investigated.Since graphene platelets are prone to agglomeration,a multi-layer distribution is adopted here to match the engineering requirements.Firstly,an equivalent composite plate model is obtained,and then nonlinear equations of motion are derived by the von Kármán nonlinear geometric relationship and Hamilton’s principle.Afterwards,the Galerkin method and harmonic balance method are used to obtain an approximate analytical solution.Results show that the unit cell half period,unit cell inclination angle,unit cell height,graphene platelet dispersion pattern and graphene platelet weight fraction and geometry play important roles in the nonlinear vibration of the GPRCC plates.
基金the University of Kashan.(Grant Number:467893/0655)。
文摘The bending and stress analysis of a functionally graded polymer composite plate reinforced with graphene platelets are studied in this paper.The governing equations are derived by using principle of virtual work for a plate which is rested on Pasternak’s foundation.Sinusoidal shear deformation theory is used to describe displacement field.Four different distribution patterns are employed in our analysis.The analytical solution is presented for a functionally graded plate to investigate the influence of important parameters.The numerical results are presented to show the deflection and stress results of the problem for four employed patterns in terms of geometric parameters such as number of layers,weight fraction and two parameters of Pasternak’s foundation.
文摘McEver和Berilacqua等将一组结构、功能相似的具有植物血凝素样结构的超家族称为选择素,它属于粘附分子。血小板上的选择素超家族便是血小板α颗粒膜蛋白140(α-granule membrane protein 140,GMP—140)。它存在于血小板的α颗粒膜上,只是在血小板被激活时才在血小板表面表达,因此它成为反映血小板活化的特异性指标。随着单克隆抗体及分子生物学技术等的应用,对GMP—140的特性、结构等已有一定的认识。在临床方面,抗人活化血小板表面GMP—140单克隆抗体的应用,一方面可直接判断体内血小板的活化程度。
文摘Objective To develop a plasma pharmacological method evaluates the effect of Da Huang Zhe Chong capsule on platelet aggregation and its mechanism, which is a representative Traditional Chinese Medicine Patent Prescription Promoting blood circulation by removing blood stasis. Methods Platelets specimens from healthy volunteers made serum and plasma with medicine, while platelet PRP were separated, which were divided into 8groups,i.e. auto-serum, allo-serum, serum with Da Huang Zhe Chong capsule , serum with aspirin, auto-plasma, plasma with Da Huang Zhe Chong capsule, plasma with aspirin, every group added to serum and plasma to hatch. After ADP and adrenalin were added into the specimens and hatched, the effects of specimens on platelet aggregation were observed. Results After ADP adrenalin were added, all the serum groups did not present platelet aggregation,while all the plasma group presented platelet aggregation. P1, P5, Pmax, t and TM have no significant difference (P＞0. 05) between auto-plasma group and allo-plasma group induced by ADP and adrenalin. P1, P5, t and Pmax have significant differences (P＜0. 01) and TM decreased significantly (P＜0. 05) comparing plasma group with Da Huang Zhe Chong capsule and plasma group of aspirin to allo-plasma group. P1, t and Pmax have significant difference (P＜0.05), and P5 and TM are simulate comparing plasma group with Da Huang Zhe Chong capsule to plasma group of aspirin. P1, P5, t and Pmax have significant differences (P＜0. 01), P1, TM have also significant(P＜0. 05), comparing plasma group of Da Huang Zhe Chong capsule with plasma of aspirin to allo-plasma group induced by adrenalin. P1 ,P5 and Pmax have significant differences (P＜0.05), and t and Pmax are simulate comparing plasma group with Da Huang Zhe Chong capsule with plasma group of aspirin. Conclusion The serum pharmacological study is inappropriate to study platelet aggregation in vitro. The plasma pharmacological study is inappropriate to study platelet aggregation in vitro. The plasma pharmacological study could reflect the pharmacological effect produced in vivo. Da Huang Zhe Chong capsule has better anti artery thrombosis effect than aspirin, and it is an ideal medicine for anti artery thrombosis.
基金supported by National Natural Science Fund of China(81273574) Chinese Herb Key Project by Health and Family Planning Commission of Hubei Province
文摘OBJECTIVE Salvia miltiorrhiza bunge contains various active constituents,some of which have been developed as commercially available medicine.Moreover,some other ingredients in Salvia miltiorrhiza play great roles in anti-platelet activity.The aim of the present study was to investigate the effects and the underlying mechanism of miltirone,a lipophilic compound of Salvia miltiorrhiza Bunge.METHODS The ability of miltirone to modulate platelet function was investigated by a variety of in vitro and in vivo experiments.Platelet aggregation and dense granule secretion induced by various agonists were measured with platelet aggregometer.Clot retraction and spreading were imaged by digital camera and fl uorescence microscope.Ferric chloride-induced carotid injury model and pulmonary thromboembolism model were used to check miltirone effect in vivo.To elucidate the mechanisms of anti-platelet activity of miltirone,flow cytometry and Western blotting were performed.RESULTS Miltirone(2,4,8 μmol·L^(-1)) was shown to suppress platelet aggregation,dense granule and α granule secretion in a dose-dependent manner.Meanwhile,miltirone inhibited the clot retraction and spreading of washed platelets.It reduced the phosphorylation of PLCγ2,PKC,Akt,GSK3β and ERK1/2 in the downstream signal pathway of collagen receptor.It also reduced the phosphorylation of Src and FAK in the integrin αⅡbβ3 mediated "outside-in" signaling,while it did not suppress the phosphorylation of β3.In addition,miltirone prolonged the occlusion time and reduced collagen/epinephrine induced pulmonary thrombi.CONCLUSION Miltirone suppresses platelet "inside-out" and "outside-in" signaling by affecting PLCγ2/PKC/ERK1/2,PI3K/Akt and Src/FAK signaling.Therefore,miltirone might represent a potential anti-platelet candidate for the prevention of thrombotic disorders.
文摘Abstract：Aim To investigate the effect of hydrogen-rich saline on arterial thrombosis in sinoaortic denervatted （SAD） rats. Methods Rats were divided randomly into Sham group, SAD group, SAD ＋ hydrogen-rich saline （5 mL · kg^-1 · d^-1） group and SAD ＋ hydrogen-rich saline （10 mL · kg^-1 · d^-1） group. Four weeks after Sham or SAD operation, rats were given intraperitoneally normal saline or hydrogen-rich saline for 4 weeks. Carotid artery thrombosis was induced by 30% ferric chloride. Time till occlusion was measured by Laser Doppler Flowme- try. Results When compared with the Sham group, time till occlusion was significantly shorter in SAD group. Moreover, platelet aggregation, platelet adhesion to collagen and the level of ROS and malondialdehyde （MDA） in plasma and Ca2＋ in platelets were significantly raised. The level of NO and SOD in plasma and NO in platelet were significantly reduced. When compared with the SAD group, time till occlusion in hydrogen-rich saline group group was significantly prolonged. Platelet aggregation, adhesion, the level of ROS, MDA and Ca2＋ were significantly re- duced, whereas the level of NO and SOD in plasma and NO in platelets were significantly higher. Conclusion Hydrogen-rich saline might improve arterial thrombosis in SAD rats through inhibition of platelet activation and oxi- dative stress.
文摘Platelets aggregation and thrombosis formation are major reasons of cardiovascular and cerebral vascular diseases.To develop new generative,potent and safe agents for inhibiting platelet aggregation and preventing above diseases are urgently required.Some traditional Chinese medicines of″Houxue Huayu″have been shown to inhibit platelet aggregation potently.In the present study the mechanisms and the molecular targets of puerarin,salvianolic acid B and the analogue of 3-n-butylphthalide,dl-PHPB were investigated and compared with ticlopidine.Four platelet aggregation inducers,ADP,arachidonic acid,collagen and thrombin were used in the study.It was found that puerarin and dl-PHPB specifically inhibited ADP induced platelet aggregation like ticlopidine did.However,salvianolic acid B inhibited both ADP and collagen induced platelet aggregations with similar potency.Due to existing two ADP receptor subtypes on platelets,P2Y1 and P2Y12,we studied the action of above compounds on the receptors and the signaling pathways.It was found that dl-PHPB decreased IP1 accumulation produced by ADP,but had no effect on IP1 level induced by m-3M3 FBS,an activator of PLC.M-3M3 FBS might attenuate the inhibitory effect of dl-PHPB on ADP-induced platelet aggregation.In addition,dl-PHPB did not affect cyclic AMP formation in platelets by ADP,which is different from P2Y12 antagonist ticlopidine.Puerarin showed the similar effects of dl-PHPB.Therefore,the actions of dl-PHPB and puerarin might be through P2Y1receptor-PLC-βpathway.Salvianolic acid B did not reduce the IP1 accumulation stimulated by ADP.It might act on the receptor subtype P2Y12.Our results suggest that components of Chinese herb medicine might be a resource for development of novel anti-platelet drugs.
文摘Aim To evaluate the time-effect and dose-effect of prasugrel hydrobromide acetic acid compound （PHAAC） inhibiting platelet aggregation. Methods For the time-effect study, 190 Sprague-Dawley （SD） rats were devided into 19 groups （n- 10）： the vehicle control group, the PHAAC groups （0.5, 1, 2, 4, 6, 24, 48, 72, 96 h） and the prasugrel hydrochloride groups （0.5, 1, 2, 4, 6, 24, 48, 72, 96 h）. Rats were singly intra- gastic administration of the vehicle, the PHAAC （5 mg·kg^-1） or the prasugrel hydrochloride （5 mg · kg^-1 ）, re- spectively. Blood samples were taken at each time point for the determination of platelet aggregation rate （PAR）. For the dose-effect study, 110 SD rats were devided into 11 groups （n= 10）： the vehicle control group, the PHAAC groups （10, 5, 2.5, 1, 0.5 mg · kg^-1, dosage of prasugrel） and the prasugrel hydrochloride groups （ 10, 5, 2.5, 1, 0.5 mg · kg^-1, dosage of prasugrel） . Blood samples were taken at 4 h after drug administration for the determination of PAR. Results Compared with the vehicle group, PHAAC has significant anti-platelet ag- gregative effects （P 〈 0.05） at the time of 0.5, 1, 2, 4, 6, 24, 48 h, and the effect at the time of 4 h was the strongest. There were no obvious differences between the effect of PHAAC （5 mg · kg^-1） and prasugrel hydrochlo- ride （5 mg · kg^-1） at each time point. Compared with the vehicle group, intragastic administration of PHAAC at the doses of 10, 5, 2.5, 1, 0.5 mg · kg^-1 could obviously inhibite the platelet aggregation, and showed a dose- dependent manner. There were no significant differences between the effect of PHAAC and prasugrel hydrochloride at the same dose. Conclusion PHAAC can inhibit platelet aggregation in a dose-dependent manner, and the effect at 4 h after drug administration is the strongest. The action strength and duration of PHAAC are similar with that of the prasugrel hydrochloride.
基金The project supported National Natural Science Foundation of China(81173061)the Collaborative Innovation Center for Research and Development of Traditional Chinese Medicine in Mount Tai+2 种基金the Foundation of Overseas Distinguished Taishan Scholars of Shandong Province(FODTS)the Natural Science Foundation of Shandong Province(ZR2014HQ007)the Science and Technology Project of Taian City(201440774-25)
文摘OBJECTIVE To investigate the effect of hydrogen-rich saline on platelet activation in hypertensive rats.METHODS The male Wistar rats were divided into control group,hypertension group,control+hydrogen-rich saline group and hypertension+hydrogen-rich saline group.Hypertension was induced by subcutaneous infusion with angiotensinⅡ0.7 mg·kg-1·d-1for 2 weeks by osmotic mini-pumps in rats.Hydrogen-rich saline(10 mL·kg-1·d-1)was administered by intraperitoneal injection for 14 d.Platelet adhesion on collagen surface was evaluated using a well-defined perfusion chamber at low shear rate(300s-1)and high shear rate(1080s-1).The maximum aggregation rate of platelets induced by ADP was determined by turbidimetry.The levels of reactive oxygen species(ROS),nitric oxide(NO)and Ca2+in platelets were measured with flow cytometry.RESULTS When compared with the control group,platelet aggregation,platelet adhesion rate in high shear rate and low shear rate,and the level of ROS and Ca2+in platelets were elevated in hypertensive group.However,NO level in platelets decreased.Compared with the hypertensive group,hydrogen-rich saline treatment decreased platelet aggregation,platelet adhesion rate,the levels of ROS and Ca2+in platelets,and increased NO level in platelets of hypertensive rats.CONCLUSION Hydrogen-rich saline could inhibit platelet activation in hypertensive rats.This effect may be related to antioxidative stress.
文摘Fetal liver tissues obtained from 28 human fetuses with gestation age from 3 to 6 months and fetal bone marrow from 35 human fetuses from 3 to 7 months were observed by immunochemical staining with anti-platelet GPⅡ b / Ⅲa monoclonal antibody and ABC technique. In the fetal liver, megakaryocytes were wholly located among growing fetal liver cells and near foci of hemopoiesis. Some megakaryocytes in the fetal liver were small7890- lymphoid-like megakaryocytes. The size of megakaryocytes both in the fetal liver (14.79 ± 4.52μm) and in the fetal bone marrow (16.08±7.39 μm) was small, which did not vary significantly over the gestation age ranging from 3 to 6 or 7 months. However, the maturation stage of megakaryocytes in the fetal liver shifted to more mature stage with the advancement of gestation, although the maturation stage of megakaryocytes in the fetal bone marrow did not change with the advancement of gestation from 4 to 7 months, the megakaryocyte in the fetal bone marrow was less mature
文摘Background and Objective Renal insufficiency(RI)is reported to be associated with increased ischemic and bleeding events after percutaneous coronary intervention(PCI),which is possibly due to high residual platelet reactivity(HRPR)during DAPT therapy.Therefore,we performed a large prospective observational study to evaluate the platelet reactivity and related clinical outcomes in real-world patients with different renal insufficiency stage after PCI,and to examine whether HRPR is associated with higher incidence of adverse cardiovascular events in a 2-year follow up.
基金Natural Science Foundation of Shandong Province(ZR2017MH048)。
文摘OBJECTIVE Platelets play a major role in mediating inflammatory response.The present work was designed to investigate whether arterial baroreflex impairment induced by sinoaortic denervation(SAD)affect platelet activation,leading to the exacerbation of cerebral cortex and hippocampus inflammation in rats.METHODS Adult male SD rats were divided into four groups:the sham control,the sinoaortic denervation(SAD),the sham+LPS,the SAD+LPS.In another experiment,there were also four groups:the sham control,the SAD,the SAD+LPS and the SAD+LPS+asprin.Four weeks after sham or SAD surgery,all rats were examined for the level of CD41,CD45,IL-1βand PF-4 in the cerebral cortex and hippocampus using immunofluorescence and ELISA.Blood platelet and leukocyte count,platelet microaggre⁃gation,expression of CD154 and CD62P on platelet surface and platelet-leukocyte aggregate level was detected by flow cytometry.RESULTS Compared with sham+LPS group,the in SAD+LPS group rats exhibited the high level of CD41,CD45,IL-1βand PF-4 in the cerebral cortex and hippocampus.Leukocyte count,platelet microag⁃gregation,expression of CD154 and CD62P on platelet surface and platelet-leukocyte aggregate level was increased,while blood platelet count was decreased in the SAD+LPS.Moreover,all the above changes were improved in the SAD+LPS+asprin group when compared with the SAD+LPS group.CONCLUSION Arterial baroreflex dysfunction exacerbates inflammation in the rat cerebral cortex and hippocampus,which is likely mediated by platelet.
文摘OBJECTIVE To screen the active ingredients preventing platelet aggregations from Taohong Siwu Decoction. METHODS Fresh blood from healthy adult volunteer was collected with vacuum tube for preparation of platelet suspension. Platelet suspension was incubated with Taohong Siwu Decoction for one hour at 37℃,then centrifuged. The precipitate was washed and desorbed with buffer solutions.The differences of chemical constituents between incubated and unincubated were identified with high performance liquid chromatography-diode array detector. RESULTS According to the chromatograph at 254 nm,at least three kinds of different components were detected in Taohong siwu decoction before and after incubated with platelet. CONCLUSION Platelet suspension can be used for screening active ingredients on platelet membrane receptor from the traditional Chinese medicine.
文摘Background and Objective The white blood cell count to mean platelet volume ratio(WMR)has recently been described as a predictor of cardiovascular events in patients who undergo percutaneous coronary intervention(PCI).The aim of this study was to investigate the usefulness of admission WMR in predicting outcomes in patients with acute coronary syndrome(ACS).
文摘Arsenic in drinking water is a worldwide health problem that is associated with cardiovascular disease, but the cause is currently unknown. In order to examine whether arsenic affects vasomotor tone in blood vessels, we investigated the effect of arsenic on agonist-induced vasorelaxation and vasoconstriction using the isolated rat aortic rings in in vitro organ bath system. Treatment with inorganic arsenite (AsⅢ) inhibited acetylcholine-induced relaxation of aortic rings by inhibiting production of nitric oxide in endothelium.
基金supported by Grants-in-Aid from the National Natural Science Foundation of China No.10632010,30670517
文摘The size mismatch in an end-to-end vascular anastomosis between the host vessel and the graft may cause flow disturbance and predispose to thrombosis [1].Although a number of techniques have been employed to reduce the risk of anastomotic thrombosis due to the size mismatch。
