The purpose of this study was to evaluate the effect and the potential mechanism of administering a pGRF gene plasmid on the growth and immunological function of weanling piglets subjected to immune-stress.Eighteen we...The purpose of this study was to evaluate the effect and the potential mechanism of administering a pGRF gene plasmid on the growth and immunological function of weanling piglets subjected to immune-stress.Eighteen weanling(Duroc×Landrace×Large White) piglets aged 35 d±2 d and initial BW of 7.86 kg±0.59 kg were randomly assigned to three treatments according to gender and BW by using a single factor design.The three treatments were injections of a pGRF gene plasmid,pGRF gene plasmid followed by challenge with lipopolysaccharide(LPS),and LPS to piglets not receiving the plasmid.Each treatment group consisted of six piglets.The results were as follows:piglets in the pGRF gene plasmid plus LPS treatment had a better growth performance than those only receiving LPS(P【0.05), and F/G of piglets in the pGRF gene plasmid plus LPS group were very slightly lower(P】0.05) than those in the LPS group;serum levels of IGF-1 in the pGRF gene plasmid plus LPS group were significantly higher than those in the LPS group(P【0.05 or P【0.01);serum levels of IgG in the pGRF gene plasmid plus LPS group were higher than those in the LPS group(P【0.05);serum levels of IL-1 and IL-6 in the pGRF gene plasmid plus LPS group were significantly lower than those in the LPS group(P【0.05 or P【0.01).展开更多
In search of the effect of direct cardiac compression and at the same time the exemption of conventional thoracotomy for cardiopulmonary resuscitation (CPR), we have invented intra-thoracic heart presser (ITHP). The I...In search of the effect of direct cardiac compression and at the same time the exemption of conventional thoracotomy for cardiopulmonary resuscitation (CPR), we have invented intra-thoracic heart presser (ITHP). The IHTP is designed to be inserted via a s展开更多
The experiment was conducted to study the effects and possible mechanism of GLP-2 on proliferation,metabolism and apoptosis of cultured enterocytes from a 28-d weaned piglet injured by exposure to β-conglycinin.A cel...The experiment was conducted to study the effects and possible mechanism of GLP-2 on proliferation,metabolism and apoptosis of cultured enterocytes from a 28-d weaned piglet injured by exposure to β-conglycinin.A cell damage model was established to investigate cell proliferation, metabolism and apoptosis by exposing primary cell cultures of intestinal epithelial cells(IEC) to 1.2 and 2.4 mg/mL β-conglycinin.A 2×3 factorial experiment was then used to study the effect of different GLP-2 concentrations of(1×10<sup>-9</sup>,1×10<sup>-8</sup> and 1×10<sup>-7</sup>mol/L),in combination with the two concentrations ofβ-conglycinin.Cells exposed to the allergenβ-conglycinin had decreased(P【0.05) MTT OD;decreased (P【0.01) protein retention and total protein content of cells;increased(P【0.01) LDH and caspase-3 activities and decreased(P【0.05) Na<sup>+</sup>,K<sup>+</sup>-ATPase activity.When GLP-2 was used in combination withβ-conglycinin,MTT OD,protein retention,total protein content and Na<sup>+</sup>,K<sup>+</sup>-ATPase activity significantly increased(P【0.05);LDH activity gradually decreased(P【0.05 or P【0.01) and Caspase-3 activity significantly decreased(P【0.01) with increasing concentrations of GLP-2.The results indicated thatβ-conglycinin had adverse effects on proliferation and integrity of IEC in vitro.GLP-2 relieved or prevented the adverse effects ofβ-conglycinin on proliferation and integrity of IEC by regulating Na<sup>+</sup>,K<sup>+</sup>- ATPase and Caspase-3 activities,and consequently affecting cell metabolism.展开更多
文摘The purpose of this study was to evaluate the effect and the potential mechanism of administering a pGRF gene plasmid on the growth and immunological function of weanling piglets subjected to immune-stress.Eighteen weanling(Duroc×Landrace×Large White) piglets aged 35 d±2 d and initial BW of 7.86 kg±0.59 kg were randomly assigned to three treatments according to gender and BW by using a single factor design.The three treatments were injections of a pGRF gene plasmid,pGRF gene plasmid followed by challenge with lipopolysaccharide(LPS),and LPS to piglets not receiving the plasmid.Each treatment group consisted of six piglets.The results were as follows:piglets in the pGRF gene plasmid plus LPS treatment had a better growth performance than those only receiving LPS(P【0.05), and F/G of piglets in the pGRF gene plasmid plus LPS group were very slightly lower(P】0.05) than those in the LPS group;serum levels of IGF-1 in the pGRF gene plasmid plus LPS group were significantly higher than those in the LPS group(P【0.05 or P【0.01);serum levels of IgG in the pGRF gene plasmid plus LPS group were higher than those in the LPS group(P【0.05);serum levels of IL-1 and IL-6 in the pGRF gene plasmid plus LPS group were significantly lower than those in the LPS group(P【0.05 or P【0.01).
文摘In search of the effect of direct cardiac compression and at the same time the exemption of conventional thoracotomy for cardiopulmonary resuscitation (CPR), we have invented intra-thoracic heart presser (ITHP). The IHTP is designed to be inserted via a s
基金supported by Program for Changjiang Scholars and Innovative Reseach Team in University (IRTO 555)Applied Basic Research(045Y029-031) of Sichuan Province,People's Republic of China
文摘The experiment was conducted to study the effects and possible mechanism of GLP-2 on proliferation,metabolism and apoptosis of cultured enterocytes from a 28-d weaned piglet injured by exposure to β-conglycinin.A cell damage model was established to investigate cell proliferation, metabolism and apoptosis by exposing primary cell cultures of intestinal epithelial cells(IEC) to 1.2 and 2.4 mg/mL β-conglycinin.A 2×3 factorial experiment was then used to study the effect of different GLP-2 concentrations of(1×10<sup>-9</sup>,1×10<sup>-8</sup> and 1×10<sup>-7</sup>mol/L),in combination with the two concentrations ofβ-conglycinin.Cells exposed to the allergenβ-conglycinin had decreased(P【0.05) MTT OD;decreased (P【0.01) protein retention and total protein content of cells;increased(P【0.01) LDH and caspase-3 activities and decreased(P【0.05) Na<sup>+</sup>,K<sup>+</sup>-ATPase activity.When GLP-2 was used in combination withβ-conglycinin,MTT OD,protein retention,total protein content and Na<sup>+</sup>,K<sup>+</sup>-ATPase activity significantly increased(P【0.05);LDH activity gradually decreased(P【0.05 or P【0.01) and Caspase-3 activity significantly decreased(P【0.01) with increasing concentrations of GLP-2.The results indicated thatβ-conglycinin had adverse effects on proliferation and integrity of IEC in vitro.GLP-2 relieved or prevented the adverse effects ofβ-conglycinin on proliferation and integrity of IEC by regulating Na<sup>+</sup>,K<sup>+</sup>- ATPase and Caspase-3 activities,and consequently affecting cell metabolism.
