Populus alba is a foundation species in evolutionary and ecological studies in the northern hemisphere.In this study,the chloroplast genome and gene map of P.alba were constructed.The P.alba chloroplast genome is 156,...Populus alba is a foundation species in evolutionary and ecological studies in the northern hemisphere.In this study,the chloroplast genome and gene map of P.alba were constructed.The P.alba chloroplast genome is 156,505 bp in length comprising a large single-copy region,two inverted repeat regions and a small single-copy region.The genome contains 131 genes,including 86 protein-coding genes(77 PCG species),eight ribosomal RNA genes(four rRNA species)and 37 transfer RNA genes(30 tRNA species).Phylogenetic analysis indicates that all Populus chloroplast genome sequences are clustered together and divided into three large branches.Among reported Populus chloroplast genomes,the leuce section formed monophyletic,indicating that all Populus spp.have a common maternal ancestor.P.rotundifolia and P.tremula are closely related and are sisters to P.davidiana.P.alba is closely related to P.adenopoda.Population genetic research in ecology and evolution may be easily developed through chloroplast genomes as they are conserved.This research will benefit future studies related to Populus,one of the world’s most ecologically and economically important genera.展开更多
Five Larix species(L.griffithii,L.speciose,L.himalaica,L.kongboensis,and L.potaninii var.australis),have survived on the Qinghai-Tibet Plateau(QTP)under specific climate conditions for decades.The lack of genomic info...Five Larix species(L.griffithii,L.speciose,L.himalaica,L.kongboensis,and L.potaninii var.australis),have survived on the Qinghai-Tibet Plateau(QTP)under specific climate conditions for decades.The lack of genomic information seriously hinders research on the evolution,conservation and ecology of these Larix resources.In this study,complete chloroplast(cp)genomes of the 5 species were assembled and compared based on next generation sequencing technology combined with polymerase chain reaction validation.The results show that the 5 cp genomes are relatively conservative in size,gene content and arrangement,and border variation.Phylogenetic analysis showed that the species are closely related as well as to seven other species of the same genus.In addition,the 5 cp genomes contained few simple sequence repeats and relatively low nucleotide variability;thus,12 candidate polymorphic cp DNA markers will be helpful for further research on relevant population genetics.These results will provide valuable genetic information for the conservation,evolution and ecology of these species and their relatives.展开更多
基金The work was supported by the Fundamental Research Funds of the Chinese Academy of Forestry(No.CAFYBB2017ZX001-1)the National Natural Science Foundation of China(No.31470665).
文摘Populus alba is a foundation species in evolutionary and ecological studies in the northern hemisphere.In this study,the chloroplast genome and gene map of P.alba were constructed.The P.alba chloroplast genome is 156,505 bp in length comprising a large single-copy region,two inverted repeat regions and a small single-copy region.The genome contains 131 genes,including 86 protein-coding genes(77 PCG species),eight ribosomal RNA genes(four rRNA species)and 37 transfer RNA genes(30 tRNA species).Phylogenetic analysis indicates that all Populus chloroplast genome sequences are clustered together and divided into three large branches.Among reported Populus chloroplast genomes,the leuce section formed monophyletic,indicating that all Populus spp.have a common maternal ancestor.P.rotundifolia and P.tremula are closely related and are sisters to P.davidiana.P.alba is closely related to P.adenopoda.Population genetic research in ecology and evolution may be easily developed through chloroplast genomes as they are conserved.This research will benefit future studies related to Populus,one of the world’s most ecologically and economically important genera.
基金the National Natural Science Foundation of China(31660215)the Construction Project for First-Class Ecology Discipline in Guizhou(GNYL[2017]007),ChinaMajor Scientifi c and Technological Projects of Guizhou Province([2018]5261),China。
文摘Five Larix species(L.griffithii,L.speciose,L.himalaica,L.kongboensis,and L.potaninii var.australis),have survived on the Qinghai-Tibet Plateau(QTP)under specific climate conditions for decades.The lack of genomic information seriously hinders research on the evolution,conservation and ecology of these Larix resources.In this study,complete chloroplast(cp)genomes of the 5 species were assembled and compared based on next generation sequencing technology combined with polymerase chain reaction validation.The results show that the 5 cp genomes are relatively conservative in size,gene content and arrangement,and border variation.Phylogenetic analysis showed that the species are closely related as well as to seven other species of the same genus.In addition,the 5 cp genomes contained few simple sequence repeats and relatively low nucleotide variability;thus,12 candidate polymorphic cp DNA markers will be helpful for further research on relevant population genetics.These results will provide valuable genetic information for the conservation,evolution and ecology of these species and their relatives.
