Cotton bollworm(He1icoverpa armigera)is an important agricultural pest that causes severe yield loss to crops,particularly to cotton.Transgenic Bt crops have been successful in protecting plant s,however,Bt proteins a...Cotton bollworm(He1icoverpa armigera)is an important agricultural pest that causes severe yield loss to crops,particularly to cotton.Transgenic Bt crops have been successful in protecting plant s,however,Bt proteins are to xic to all lepidopteran insects but have little effects to sucking pests,such as aphids.Furthermore,the continuo us use of Bt crop s increases insect resistance.These call for new transgenic app roac hes of pest control.The bollworms have adapted to cotto n plants,de-spite that most cotton cultivars accumulate a high level of gossypol and related sesquiterpene p hytoa-lexins.展开更多
Background Apolygus lucorum is a worldwide omnivorous pest damaging a range of crops and causing great economic losses.Symbiotic bacteria living in insects play a key role in the nutrition,physiology,and behavior of h...Background Apolygus lucorum is a worldwide omnivorous pest damaging a range of crops and causing great economic losses.Symbiotic bacteria living in insects play a key role in the nutrition,physiology,and behavior of hosts.Here,we present an experiment using Illumina HiSeq sequencing targeting the V3–V4 regions of bacteria’s 16S rRNA throughout the entire life cycle of A.lucorum.Results The first and second instar nymphs have the largest alpha diversity compared with other life stages of the insect.Bacterial phyla Proteobacteria(72.29%),Firmicutes(15.24%),Actinobacteria(7.76%)exhibit the largest relative abundance in all developmental stages.Erwinia(23.97%)and Lactococcus(10.62%)are the two genera with the high-est relative abundance.The relative abundance of Erwinia in the nymph stage is significantly greater than the adult stage,and the relative abundance of Lactococcus in 6-day-old and 9-day-old adult females is higher compared with adult males.Conclusions These results reveal that microbial community composition and relative abundance shift dynamically at different life stages,implying that different bacterial phyla and genera may have specific roles in specific life stages such as metabolism,nutrition absorption,detoxification,and reproduction.This study reveals for the first time the community composition and ecological dynamics of symbiotic bacteria throughout the life stages of A.lucorum,and thus may provide insight to new strategies for pest control.展开更多
文摘Cotton bollworm(He1icoverpa armigera)is an important agricultural pest that causes severe yield loss to crops,particularly to cotton.Transgenic Bt crops have been successful in protecting plant s,however,Bt proteins are to xic to all lepidopteran insects but have little effects to sucking pests,such as aphids.Furthermore,the continuo us use of Bt crop s increases insect resistance.These call for new transgenic app roac hes of pest control.The bollworms have adapted to cotto n plants,de-spite that most cotton cultivars accumulate a high level of gossypol and related sesquiterpene p hytoa-lexins.
基金This research was supported by Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences.
文摘Background Apolygus lucorum is a worldwide omnivorous pest damaging a range of crops and causing great economic losses.Symbiotic bacteria living in insects play a key role in the nutrition,physiology,and behavior of hosts.Here,we present an experiment using Illumina HiSeq sequencing targeting the V3–V4 regions of bacteria’s 16S rRNA throughout the entire life cycle of A.lucorum.Results The first and second instar nymphs have the largest alpha diversity compared with other life stages of the insect.Bacterial phyla Proteobacteria(72.29%),Firmicutes(15.24%),Actinobacteria(7.76%)exhibit the largest relative abundance in all developmental stages.Erwinia(23.97%)and Lactococcus(10.62%)are the two genera with the high-est relative abundance.The relative abundance of Erwinia in the nymph stage is significantly greater than the adult stage,and the relative abundance of Lactococcus in 6-day-old and 9-day-old adult females is higher compared with adult males.Conclusions These results reveal that microbial community composition and relative abundance shift dynamically at different life stages,implying that different bacterial phyla and genera may have specific roles in specific life stages such as metabolism,nutrition absorption,detoxification,and reproduction.This study reveals for the first time the community composition and ecological dynamics of symbiotic bacteria throughout the life stages of A.lucorum,and thus may provide insight to new strategies for pest control.
