The UV-Vis and infrared(IR)absorption spectra of organophosphorus(OP)pesticides have been studied.A correlation in spectra was developed to optimize the OP pesticides in the environments.The spectroscopic(UV-Vis and I...The UV-Vis and infrared(IR)absorption spectra of organophosphorus(OP)pesticides have been studied.A correlation in spectra was developed to optimize the OP pesticides in the environments.The spectroscopic(UV-Vis and IR)spectrum of OP pesticides like methyl parathion,malathion and parathion has been interpreted in detail.A complete calculation of the normal frequencies and absolute intensities of UV-Vis and IR absorption bands are interpreted with the help of corresponding experimental data.In the colorimetric reactions,the bands appear at 2 077,1 637,1 455,1 015,655cm^(-1) for malathion;2 081,1 639,1 316,1 015,794,683cm^(-1) for parathion;2 078,1 632,1 032,794cm^(-1) for methyl parathion were used for quantitative or qualitative analysis.All these IR spectra were acquired by averaging 100 scans at a resolution of 4cm^(-1).It is determined experimentally in the region 200~450nm for UV-Vis absorption bands and in the region 400~4 000cm^(-1) for IR absorption bands.It is concluded that the mainly optically active groups(P—OH, C=O,P=O, C—O—C,P—O—C, P=S, —OH)present in pesticides which are responsible in change in significant data for quantitative and qualitative analysis.The various optical properties like wavelength,band energy,wave number,and frequency,also are calculated.展开更多
The Organochlorine Pesticides(OCPs),such as hexachlorocyclohexane(HCH)and dichlorodiphenyltrichloro ethane(DDT),are well known as typical persistent organic pollutions(POPs).They were worldwide used several decades ag...The Organochlorine Pesticides(OCPs),such as hexachlorocyclohexane(HCH)and dichlorodiphenyltrichloro ethane(DDT),are well known as typical persistent organic pollutions(POPs).They were worldwide used several decades ago,even today in some area.Although the water resource is very important in natural environments and essential to agriculture,the pollution of OCPs in water has emerged as worldwide environmental problem. Wuhan section of the Yangtze River is the drinking water source of Wuhan area in Hubei province.In addition,Hubei province is an important production base of grain,cotton,oil and pig.As the rapid development of industry and mass-use of OCPs展开更多
OBJECTIVE Identification of pesticide residues in TCM herbs is important for drug safety and public health.However,it is very challenging for the development of an instrument-based multi-residue pesticide analysis met...OBJECTIVE Identification of pesticide residues in TCM herbs is important for drug safety and public health.However,it is very challenging for the development of an instrument-based multi-residue pesticide analysis method for theherbalproducts due to their complex matrix.This study aims to develop a rapid method for the high-throughput detection of pesticide residues in TCM herbal products using liquid chromatography coupled to quadrupoletime of flight(LC-QTOF)mass spectrometry.METHODS In this study,we attempt to investigate possible pesticide residues in TCM herbal products which are commonly available in Singapore and to consolidate a comprehensive pesticide database for more than 250 regulated pesticides in FDA Regulations.We also attempt to perform non-targeted and targeted screenings of pesticide residues in herbal products by searching our database.RESULTS The developed LC-QTOF method demonstrates have high mass accuracy,resolution and selectivity,which makes it a very suitable tool for monitoring both target and non-target pesticides with a limit of detection at the low ppb level.Moreover,the LC-QTOF technology permits to retain all spectral data in a single run.It may avoid many difficulties related to matrix effects and will save much manpower.CONCLUSIONThis means that a variety of TCM herbs can be easily detected by this method for the presence or absence of pesticide residues that must conform to government regulations.展开更多
The levels of common pesticides and heavy metals in Dendrobium officinale grown from 2012 to 2014 were measured, and three planting methods were used in 2014. The quality of plant husbandry improved during the study p...The levels of common pesticides and heavy metals in Dendrobium officinale grown from 2012 to 2014 were measured, and three planting methods were used in 2014. The quality of plant husbandry improved during the study period. Thirteen types of pesticides were detected in D. officinale grown in 2014 and antimicrobials, including carbendazim, difenoconazole and azoxystrobin, were evident in 61.53% of plants. These three pesticides constituted 72.33% of all the antimicrobials detected. Planting methods affected the levels of pesticides and heavy metals. Trellising in plastic sheds was optimal, being associated with the lowest amounts of both pesticides and heavy metals. Both cadmium（Cd） and lead（Pb） levels were high in treed plants, whereas Cr, As and Cu levels were the highest in potted plants.展开更多
A GC-MS method for the determination of 27 organochlorine pesticides and 15 Pyrethroid pesticides in animal food is established.The method was based on Gel Permeation chromatography combined with solid-phase extractio...A GC-MS method for the determination of 27 organochlorine pesticides and 15 Pyrethroid pesticides in animal food is established.The method was based on Gel Permeation chromatography combined with solid-phase extraction for sample preparation.Rapid qualitative and quantitative analyses was carried out by gas chromatography-mass spectrometry under the selective-ion monitoring mode.The experimental results showed that the correlation coefficients were better than 0.99,the recoveries for spiked standards were 70%-104%,the relative standard deviations were 2.1%-15.9%.展开更多
A new method was developed for determination of 407 pesticide residues in complex matrix by using LC-MS with triple quadrupole(QQQ).Comparing with regular method,the method has high sensitivity and repeativity,and was...A new method was developed for determination of 407 pesticide residues in complex matrix by using LC-MS with triple quadrupole(QQQ).Comparing with regular method,the method has high sensitivity and repeativity,and was applied for the determination of peticide in real sample with satisfactory results.展开更多
The paper is to study the main co-extractions existing in multiresidue analysis of pesticides in vegetables.For this,extracted 10 vegetable and analyzed the extractions with GC-MS.Compared the spectra of those co-extr...The paper is to study the main co-extractions existing in multiresidue analysis of pesticides in vegetables.For this,extracted 10 vegetable and analyzed the extractions with GC-MS.Compared the spectra of those co-extractions in different vegetables,confirm the main co-extractions of vegetables.The result shows the co-extractions are same including pigment,phenols,fat acids,lipin and steroid.But the mass of those co-extractions in different vegetable is different.The study result is help for the development of pesticide residue method.展开更多
A new method was developed for the simultaneous analysis of 12 pesticide residues in onion and fennel by gas chromatography(GC)-triple quadrupole mass spectrometry(MS/MS)-electron impact ion source(EI)-select reaction...A new method was developed for the simultaneous analysis of 12 pesticide residues in onion and fennel by gas chromatography(GC)-triple quadrupole mass spectrometry(MS/MS)-electron impact ion source(EI)-select reaction monitoring mode(SRM).The 12 pesticide includes fungicide,acaricide,carbamate and plant growth regulator pesticide.The complicated matrix samples of onion and fennel were extracted by acetonitrile,cleaned up by solid phase extraction of Carb/PSA.By optimizing the GC-MS/MS-SRM parameters,the interference from clean-up procedure was further removed and 12 pesticide residues were determined accurately.The average recoveries for most pesticides(at the spiked level of 0.05-0.20 mg/kg) were between 114%-156%,the relative standard deviation(RSD) of the method was less than 20%,and the limit of detection were 0.5-5 μg/kg.展开更多
To establish a new GC-MS/MS method for quantitative detection of 22 kinds of pesticide in fruit and vegetable including organic phosphorus,carbamate & pyrethrin etc.Working was performed by Xcalibur software with ...To establish a new GC-MS/MS method for quantitative detection of 22 kinds of pesticide in fruit and vegetable including organic phosphorus,carbamate & pyrethrin etc.Working was performed by Xcalibur software with multitask mode.We Carried on qualitative and quantitative analysis to mass spectrum of MS/MS in order to obtained a credible experiment result.This method is sensitive and accurate,it is suitable for determination of multifarious pesticide residues.展开更多
An efficient and selective analytical method for the determination of 8 organochlorines pesticides residues(α-666,β-666,γ-666,δ-666,op’-DDT,pp’-DDT,pp’-DDD,pp’-DDE) in coffee has been developed.The cof...An efficient and selective analytical method for the determination of 8 organochlorines pesticides residues(α-666,β-666,γ-666,δ-666,op’-DDT,pp’-DDT,pp’-DDD,pp’-DDE) in coffee has been developed.The coffee samples were extracted with hexane-acetone(1 ∶1,by volume) according to a selective extraction step using accelerated solvent extraction(ASE),followed by a clean-up by gel permeation chromatography(GPC) and a column combined with Florisil and GCB.Identification and quantification were performed using gas chromatography with negative chemical ionization mass spectrometric detection(GC-NCI MS).The method detection limits varied between 0.002 and 0.069 ng/g.The calibration curves showed a good linearity for all OCPs(r≥0.999 5) under a concentration of 0.20-50.0 μg/L.When the spiked levels were 0.5 μg/L and 5 μg/L,the average recoveries were range of 94%-110% with RSD of 3.5%-10.6%.The method was accurate,sensitive and suitable for the analysis of pesticides.展开更多
The spectrum user’s librarys containing 1533 familiar toxicants have been built; A method with high separability and sensitivity has been found,The RT and the detection limit of 210 familiar abuse drugs has been conf...The spectrum user’s librarys containing 1533 familiar toxicants have been built; A method with high separability and sensitivity has been found,The RT and the detection limit of 210 familiar abuse drugs has been confirmed; the processing methods to analyse spectrum data automatically have been found; It is simple,quick,sensitive and reliable,and can take place of manual operation,It is very useful to screen the familiar toxicants in unkown sample.展开更多
A high-performance liquid chromatography-electrospray tandem quadrupole mass spectrometry with linear trap detection method for the quantify and determination of a lot of pesticide residues in vegetable is described. ...A high-performance liquid chromatography-electrospray tandem quadrupole mass spectrometry with linear trap detection method for the quantify and determination of a lot of pesticide residues in vegetable is described. The pesticide were extracted from more vegetable(such as new ginger,garlic,shallot) with acetonitrile and the extract purified by SPE(solid phase extract). To set up EPI(enhance product ion) MS library.LC-MS/MS limit of detection (LOD) based on a signal/noise ratio of 3,was estimated to be 1 μg/kg for all pesticides. The overall average recovery in twenty-five compounds spiked vegetable was 70%-115%. LC-MS/MS-QTRAP has shown itself to be a senitive,selective and rapid method and was successfully used for the quantify and confirmation analysis of more pesticides in more difference vegetable samples for regulatory purposes.展开更多
The screening of pesticide residues plays a vital role in food safety.Applications of high throughput analytical procedures are desirable for screening a large number of pesticides and food samples in a time-efficient...The screening of pesticide residues plays a vital role in food safety.Applications of high throughput analytical procedures are desirable for screening a large number of pesticides and food samples in a time-efficient and cost-effective manner.This review discusses how sample throughput of pesticide analysis could be improved with an emphasis on sample preparation,instrumentation and data analysis.展开更多
Water is an essential natural resource for sustaining life and environment.Though groundwater contributes only 0.6%of the total water resources on earth, it is the major and preferred source of drinking water in rural...Water is an essential natural resource for sustaining life and environment.Though groundwater contributes only 0.6%of the total water resources on earth, it is the major and preferred source of drinking water in rural as well as urban areas of many developing countries like India.Uttar Pradesh state展开更多
The application of organic mass spectrometry on pesticides residues analysis was reviewed.According to the characteristics of pesticides residues,sample separation could be performed by GC or LC.The analytical charact...The application of organic mass spectrometry on pesticides residues analysis was reviewed.According to the characteristics of pesticides residues,sample separation could be performed by GC or LC.The analytical characteristics of organic mass spectrometry,including GC-MS,LC-MS,GC-tandem MS and LC-tandem MS,were discussed.展开更多
The agricultural production on the irrigated grounds can not carry on without mineral fertilizers,pesticides and herbicides.Especially it is shown in Uzbekistan, in cultivation of cotton.There is an increase in minera...The agricultural production on the irrigated grounds can not carry on without mineral fertilizers,pesticides and herbicides.Especially it is shown in Uzbekistan, in cultivation of cotton.There is an increase in mineralization,rigidity,quantity of heavy metals,phenols and other pollutions in the cotton fields.Thus there is an exhaustion of stocks of fresh underground waters.In the year 2003 we were offered to create展开更多
基金worked in funding projects from DST,New Delhi for extensive research in the area of nano-bio molecular spectroscopy and sensor technology development
文摘The UV-Vis and infrared(IR)absorption spectra of organophosphorus(OP)pesticides have been studied.A correlation in spectra was developed to optimize the OP pesticides in the environments.The spectroscopic(UV-Vis and IR)spectrum of OP pesticides like methyl parathion,malathion and parathion has been interpreted in detail.A complete calculation of the normal frequencies and absolute intensities of UV-Vis and IR absorption bands are interpreted with the help of corresponding experimental data.In the colorimetric reactions,the bands appear at 2 077,1 637,1 455,1 015,655cm^(-1) for malathion;2 081,1 639,1 316,1 015,794,683cm^(-1) for parathion;2 078,1 632,1 032,794cm^(-1) for methyl parathion were used for quantitative or qualitative analysis.All these IR spectra were acquired by averaging 100 scans at a resolution of 4cm^(-1).It is determined experimentally in the region 200~450nm for UV-Vis absorption bands and in the region 400~4 000cm^(-1) for IR absorption bands.It is concluded that the mainly optically active groups(P—OH, C=O,P=O, C—O—C,P—O—C, P=S, —OH)present in pesticides which are responsible in change in significant data for quantitative and qualitative analysis.The various optical properties like wavelength,band energy,wave number,and frequency,also are calculated.
文摘The Organochlorine Pesticides(OCPs),such as hexachlorocyclohexane(HCH)and dichlorodiphenyltrichloro ethane(DDT),are well known as typical persistent organic pollutions(POPs).They were worldwide used several decades ago,even today in some area.Although the water resource is very important in natural environments and essential to agriculture,the pollution of OCPs in water has emerged as worldwide environmental problem. Wuhan section of the Yangtze River is the drinking water source of Wuhan area in Hubei province.In addition,Hubei province is an important production base of grain,cotton,oil and pig.As the rapid development of industry and mass-use of OCPs
文摘OBJECTIVE Identification of pesticide residues in TCM herbs is important for drug safety and public health.However,it is very challenging for the development of an instrument-based multi-residue pesticide analysis method for theherbalproducts due to their complex matrix.This study aims to develop a rapid method for the high-throughput detection of pesticide residues in TCM herbal products using liquid chromatography coupled to quadrupoletime of flight(LC-QTOF)mass spectrometry.METHODS In this study,we attempt to investigate possible pesticide residues in TCM herbal products which are commonly available in Singapore and to consolidate a comprehensive pesticide database for more than 250 regulated pesticides in FDA Regulations.We also attempt to perform non-targeted and targeted screenings of pesticide residues in herbal products by searching our database.RESULTS The developed LC-QTOF method demonstrates have high mass accuracy,resolution and selectivity,which makes it a very suitable tool for monitoring both target and non-target pesticides with a limit of detection at the low ppb level.Moreover,the LC-QTOF technology permits to retain all spectral data in a single run.It may avoid many difficulties related to matrix effects and will save much manpower.CONCLUSIONThis means that a variety of TCM herbs can be easily detected by this method for the presence or absence of pesticide residues that must conform to government regulations.
文摘The levels of common pesticides and heavy metals in Dendrobium officinale grown from 2012 to 2014 were measured, and three planting methods were used in 2014. The quality of plant husbandry improved during the study period. Thirteen types of pesticides were detected in D. officinale grown in 2014 and antimicrobials, including carbendazim, difenoconazole and azoxystrobin, were evident in 61.53% of plants. These three pesticides constituted 72.33% of all the antimicrobials detected. Planting methods affected the levels of pesticides and heavy metals. Trellising in plastic sheds was optimal, being associated with the lowest amounts of both pesticides and heavy metals. Both cadmium（Cd） and lead（Pb） levels were high in treed plants, whereas Cr, As and Cu levels were the highest in potted plants.
文摘A GC-MS method for the determination of 27 organochlorine pesticides and 15 Pyrethroid pesticides in animal food is established.The method was based on Gel Permeation chromatography combined with solid-phase extraction for sample preparation.Rapid qualitative and quantitative analyses was carried out by gas chromatography-mass spectrometry under the selective-ion monitoring mode.The experimental results showed that the correlation coefficients were better than 0.99,the recoveries for spiked standards were 70%-104%,the relative standard deviations were 2.1%-15.9%.
文摘A new method was developed for determination of 407 pesticide residues in complex matrix by using LC-MS with triple quadrupole(QQQ).Comparing with regular method,the method has high sensitivity and repeativity,and was applied for the determination of peticide in real sample with satisfactory results.
文摘The paper is to study the main co-extractions existing in multiresidue analysis of pesticides in vegetables.For this,extracted 10 vegetable and analyzed the extractions with GC-MS.Compared the spectra of those co-extractions in different vegetables,confirm the main co-extractions of vegetables.The result shows the co-extractions are same including pigment,phenols,fat acids,lipin and steroid.But the mass of those co-extractions in different vegetable is different.The study result is help for the development of pesticide residue method.
文摘A new method was developed for the simultaneous analysis of 12 pesticide residues in onion and fennel by gas chromatography(GC)-triple quadrupole mass spectrometry(MS/MS)-electron impact ion source(EI)-select reaction monitoring mode(SRM).The 12 pesticide includes fungicide,acaricide,carbamate and plant growth regulator pesticide.The complicated matrix samples of onion and fennel were extracted by acetonitrile,cleaned up by solid phase extraction of Carb/PSA.By optimizing the GC-MS/MS-SRM parameters,the interference from clean-up procedure was further removed and 12 pesticide residues were determined accurately.The average recoveries for most pesticides(at the spiked level of 0.05-0.20 mg/kg) were between 114%-156%,the relative standard deviation(RSD) of the method was less than 20%,and the limit of detection were 0.5-5 μg/kg.
文摘To establish a new GC-MS/MS method for quantitative detection of 22 kinds of pesticide in fruit and vegetable including organic phosphorus,carbamate & pyrethrin etc.Working was performed by Xcalibur software with multitask mode.We Carried on qualitative and quantitative analysis to mass spectrum of MS/MS in order to obtained a credible experiment result.This method is sensitive and accurate,it is suitable for determination of multifarious pesticide residues.
文摘An efficient and selective analytical method for the determination of 8 organochlorines pesticides residues(α-666,β-666,γ-666,δ-666,op’-DDT,pp’-DDT,pp’-DDD,pp’-DDE) in coffee has been developed.The coffee samples were extracted with hexane-acetone(1 ∶1,by volume) according to a selective extraction step using accelerated solvent extraction(ASE),followed by a clean-up by gel permeation chromatography(GPC) and a column combined with Florisil and GCB.Identification and quantification were performed using gas chromatography with negative chemical ionization mass spectrometric detection(GC-NCI MS).The method detection limits varied between 0.002 and 0.069 ng/g.The calibration curves showed a good linearity for all OCPs(r≥0.999 5) under a concentration of 0.20-50.0 μg/L.When the spiked levels were 0.5 μg/L and 5 μg/L,the average recoveries were range of 94%-110% with RSD of 3.5%-10.6%.The method was accurate,sensitive and suitable for the analysis of pesticides.
文摘The spectrum user’s librarys containing 1533 familiar toxicants have been built; A method with high separability and sensitivity has been found,The RT and the detection limit of 210 familiar abuse drugs has been confirmed; the processing methods to analyse spectrum data automatically have been found; It is simple,quick,sensitive and reliable,and can take place of manual operation,It is very useful to screen the familiar toxicants in unkown sample.
文摘A high-performance liquid chromatography-electrospray tandem quadrupole mass spectrometry with linear trap detection method for the quantify and determination of a lot of pesticide residues in vegetable is described. The pesticide were extracted from more vegetable(such as new ginger,garlic,shallot) with acetonitrile and the extract purified by SPE(solid phase extract). To set up EPI(enhance product ion) MS library.LC-MS/MS limit of detection (LOD) based on a signal/noise ratio of 3,was estimated to be 1 μg/kg for all pesticides. The overall average recovery in twenty-five compounds spiked vegetable was 70%-115%. LC-MS/MS-QTRAP has shown itself to be a senitive,selective and rapid method and was successfully used for the quantify and confirmation analysis of more pesticides in more difference vegetable samples for regulatory purposes.
文摘The screening of pesticide residues plays a vital role in food safety.Applications of high throughput analytical procedures are desirable for screening a large number of pesticides and food samples in a time-efficient and cost-effective manner.This review discusses how sample throughput of pesticide analysis could be improved with an emphasis on sample preparation,instrumentation and data analysis.
文摘Water is an essential natural resource for sustaining life and environment.Though groundwater contributes only 0.6%of the total water resources on earth, it is the major and preferred source of drinking water in rural as well as urban areas of many developing countries like India.Uttar Pradesh state
文摘The application of organic mass spectrometry on pesticides residues analysis was reviewed.According to the characteristics of pesticides residues,sample separation could be performed by GC or LC.The analytical characteristics of organic mass spectrometry,including GC-MS,LC-MS,GC-tandem MS and LC-tandem MS,were discussed.
文摘The agricultural production on the irrigated grounds can not carry on without mineral fertilizers,pesticides and herbicides.Especially it is shown in Uzbekistan, in cultivation of cotton.There is an increase in mineralization,rigidity,quantity of heavy metals,phenols and other pollutions in the cotton fields.Thus there is an exhaustion of stocks of fresh underground waters.In the year 2003 we were offered to create
