This study aimed to isolate and characterize the structures of glycoproteins from peas and determine their hypoglycemic activity.The crude pea glycoproteins(PGP)were extracted by hot water and purified by diethylamino...This study aimed to isolate and characterize the structures of glycoproteins from peas and determine their hypoglycemic activity.The crude pea glycoproteins(PGP)were extracted by hot water and purified by diethylaminoethyl(DEAE)-Sepharose chromatography and Sephadex G-100 size-exclusion chromatography in sequence.Then three main fractions were obtained,namely PGP1,PGP2 and PGP3,with molecular weights of 897615,846740 and 1194692 Da,respectively.The physical and chemical properties of the three fractions were evaluated and compared by Fourier transform infrared spectroscopy(FT-IR),nuclear magnetic resonance(NMR),scanning electron microscope(SEM),high performance liquid chromatography(HPLC)and other analytical techniques.The fraction PGP2 with the highest hypoglycemic activity,was screened using the Caco-2 monolayer cell model.It can inhibit the uptake of glucose in the small intestine,as well as the activities of maltase and sucrase.After simulated gastrointestinal digestion,PGP2 signifi cantly enhanced the inhibitory effect of α-glucosidase,and slightly reduced the inhibitory ability ofα-amylase.In summary,PGP2 possessed strong hypoglycemic activity after digestion.These results indicated that PGP2 has the potential to be developed into a functional food or natural medicine for the treatment of type 2 diabetes mellitus.展开更多
We irradiated pea seeds with neutrons from a ^(252)Cf source and studied the radiation dose effects on various morphological development parameters during the growth of M_(1) generation peas.We found that in the dose ...We irradiated pea seeds with neutrons from a ^(252)Cf source and studied the radiation dose effects on various morphological development parameters during the growth of M_(1) generation peas.We found that in the dose range of 0.51-9.27 Gy,with the increase in neutron-absorbed dose,the morphological development parameters of M_(1) generation peas at the initial seedling stage showed an obvious trend with three fluctuations.With the development of pea,this trend gradually weakened.Further analysis and verification showed that the main trend in the M_(1) generation of pea seeds was an inhibitory effect induced by neutron irradiation and there was a good linear correlation between the inhibitory effect and neutron absorption dose We successfully demonstrated the background removal of mutant plants and defined morphological developmen parameters for peas that match the overall development of plants.Our results will positively impact neutron mutation breeding and automatic agriculture.展开更多
为确定对春播豌豆安全高效的除草剂种类及其剂量,采用田间小区试验方法,对5种茎叶除草剂进行防效观察及安全性评价。结果表明,供试除草剂均可用于豌豆田。其中108 g/L高效氟吡甲禾灵乳油450~600 m L/hm^2总防效最好,480 g/L灭草松水剂3 ...为确定对春播豌豆安全高效的除草剂种类及其剂量,采用田间小区试验方法,对5种茎叶除草剂进行防效观察及安全性评价。结果表明,供试除草剂均可用于豌豆田。其中108 g/L高效氟吡甲禾灵乳油450~600 m L/hm^2总防效最好,480 g/L灭草松水剂3 000~4 500 m L/hm^2、10%精喹禾灵乳油900 m L/hm^2、80%阔草清水分散粒剂45 g/hm^2和24%烯草酮乳油675 m L/hm^2均可有效防除豌豆田杂草。10%精喹禾灵乳油900 m L/hm^2和80%阔草清水分散粒剂30~45 g/hm^2处理增产效果显著,均较对照增产10%以上。108 g/L高效氟吡甲禾灵乳油450 m L/hm^2处理较对照增产9.59%。综合考虑除草效果和安全性,推荐108 g/L高效氟吡甲禾灵乳油450m L/hm^2、480 g/L灭草松水剂3 000 m L/hm^2、10%精喹禾灵乳油900 m L/hm^2和80%阔草清水分散粒剂45 g/hm^2用于豌豆田杂草防除。展开更多
基金The authors would like to show deepest gratitude tothe Earmarked Fund for Jiangsu Agricultural Industry Technology System(JATS[2020]413)Postgraduate Research&Practice Innovation Program of Jiangsu Province(No.KYCX19_0682)forfinancial assistance.
文摘This study aimed to isolate and characterize the structures of glycoproteins from peas and determine their hypoglycemic activity.The crude pea glycoproteins(PGP)were extracted by hot water and purified by diethylaminoethyl(DEAE)-Sepharose chromatography and Sephadex G-100 size-exclusion chromatography in sequence.Then three main fractions were obtained,namely PGP1,PGP2 and PGP3,with molecular weights of 897615,846740 and 1194692 Da,respectively.The physical and chemical properties of the three fractions were evaluated and compared by Fourier transform infrared spectroscopy(FT-IR),nuclear magnetic resonance(NMR),scanning electron microscope(SEM),high performance liquid chromatography(HPLC)and other analytical techniques.The fraction PGP2 with the highest hypoglycemic activity,was screened using the Caco-2 monolayer cell model.It can inhibit the uptake of glucose in the small intestine,as well as the activities of maltase and sucrase.After simulated gastrointestinal digestion,PGP2 signifi cantly enhanced the inhibitory effect of α-glucosidase,and slightly reduced the inhibitory ability ofα-amylase.In summary,PGP2 possessed strong hypoglycemic activity after digestion.These results indicated that PGP2 has the potential to be developed into a functional food or natural medicine for the treatment of type 2 diabetes mellitus.
基金supported by the National Natural Science Foundation of China(Nos.11675069 and 12075106)the Natural Science Foundation of Gansu Province(No.20JR10RA607)the Fundamental Research Funds for the Central Universities of China(No.lzujbky-2020-kb09)。
文摘We irradiated pea seeds with neutrons from a ^(252)Cf source and studied the radiation dose effects on various morphological development parameters during the growth of M_(1) generation peas.We found that in the dose range of 0.51-9.27 Gy,with the increase in neutron-absorbed dose,the morphological development parameters of M_(1) generation peas at the initial seedling stage showed an obvious trend with three fluctuations.With the development of pea,this trend gradually weakened.Further analysis and verification showed that the main trend in the M_(1) generation of pea seeds was an inhibitory effect induced by neutron irradiation and there was a good linear correlation between the inhibitory effect and neutron absorption dose We successfully demonstrated the background removal of mutant plants and defined morphological developmen parameters for peas that match the overall development of plants.Our results will positively impact neutron mutation breeding and automatic agriculture.
文摘为确定对春播豌豆安全高效的除草剂种类及其剂量,采用田间小区试验方法,对5种茎叶除草剂进行防效观察及安全性评价。结果表明,供试除草剂均可用于豌豆田。其中108 g/L高效氟吡甲禾灵乳油450~600 m L/hm^2总防效最好,480 g/L灭草松水剂3 000~4 500 m L/hm^2、10%精喹禾灵乳油900 m L/hm^2、80%阔草清水分散粒剂45 g/hm^2和24%烯草酮乳油675 m L/hm^2均可有效防除豌豆田杂草。10%精喹禾灵乳油900 m L/hm^2和80%阔草清水分散粒剂30~45 g/hm^2处理增产效果显著,均较对照增产10%以上。108 g/L高效氟吡甲禾灵乳油450 m L/hm^2处理较对照增产9.59%。综合考虑除草效果和安全性,推荐108 g/L高效氟吡甲禾灵乳油450m L/hm^2、480 g/L灭草松水剂3 000 m L/hm^2、10%精喹禾灵乳油900 m L/hm^2和80%阔草清水分散粒剂45 g/hm^2用于豌豆田杂草防除。
