Retinoic acid-inducible gene Ⅰ (RIG-Ⅰ) and melanoma differentiation-associated protein 5 (MDA5) sense viral RNA and activate antiviral immune responses.Herein we investigate their functions in human epithelial cells...Retinoic acid-inducible gene Ⅰ (RIG-Ⅰ) and melanoma differentiation-associated protein 5 (MDA5) sense viral RNA and activate antiviral immune responses.Herein we investigate their functions in human epithelial cells,the primary and initial target of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).A deficiency in MDA5,RIG-Ⅰ or mitochondrial antiviral signaling protein (MAVS) enhanced viral replication.The expression of the type I/III interferon(IFN) during infection was impaired in MDA5;and MAVS;,but not in RIG-Ⅰ;,when compared to wild type (WT) cells.The mRNA level of full-length angiotensin-converting enzyme 2 (ACE2),the cellular entry receptor for SARS-CoV-2,was approximately 2.5-fold higher in RIG-Ⅰ;than WT cells.These data demonstrate MDA5 as the predominant SARS-CoV-2 sensor,IFN-independent induction of ACE2 and anti-SARS-CoV-2 role of RIG-Ⅰ in epithelial cells.展开更多
为了解猪圆环病毒2型(porcine circovirus type 2,PCV2)感染对仔猪的肺泡巨噬细胞(PAM)免疫功能造成的影响,试验对PCV2感染40日龄左右健康仔猪PAM模式识别受体TLR2、TLR4、CD16、CD18mRNA的动态变化进行了研究。结果发现,在病毒感染后T...为了解猪圆环病毒2型(porcine circovirus type 2,PCV2)感染对仔猪的肺泡巨噬细胞(PAM)免疫功能造成的影响,试验对PCV2感染40日龄左右健康仔猪PAM模式识别受体TLR2、TLR4、CD16、CD18mRNA的动态变化进行了研究。结果发现,在病毒感染后TLR2、TLR4、CD16mRNA的转录水平动态变化基本相似,3、7d高于对照组,14d低于对照组,28d接近对照组,且3d时TLR4、CD16差异极显著(P<0.01),14d仅TLR2差异显著(P<0.05);CD18mRNA的转录水平,3d时显著低于对照组(P<0.05),而7d时显著高于对照组(P<0.05),28d接近正常。以上结果表明,PCV2感染PAM后可引起PAM对异物识别吞噬功能与吞噬后信号转导功能出现紊乱,影响了PAM吞噬和清除病原微生物的能力。展开更多
基金supported by a National Institutes of Health grant (No. R01AI132526)a UConn Health Startup fund to Wang P。
文摘Retinoic acid-inducible gene Ⅰ (RIG-Ⅰ) and melanoma differentiation-associated protein 5 (MDA5) sense viral RNA and activate antiviral immune responses.Herein we investigate their functions in human epithelial cells,the primary and initial target of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).A deficiency in MDA5,RIG-Ⅰ or mitochondrial antiviral signaling protein (MAVS) enhanced viral replication.The expression of the type I/III interferon(IFN) during infection was impaired in MDA5;and MAVS;,but not in RIG-Ⅰ;,when compared to wild type (WT) cells.The mRNA level of full-length angiotensin-converting enzyme 2 (ACE2),the cellular entry receptor for SARS-CoV-2,was approximately 2.5-fold higher in RIG-Ⅰ;than WT cells.These data demonstrate MDA5 as the predominant SARS-CoV-2 sensor,IFN-independent induction of ACE2 and anti-SARS-CoV-2 role of RIG-Ⅰ in epithelial cells.
文摘为了解猪圆环病毒2型(porcine circovirus type 2,PCV2)感染对仔猪的肺泡巨噬细胞(PAM)免疫功能造成的影响,试验对PCV2感染40日龄左右健康仔猪PAM模式识别受体TLR2、TLR4、CD16、CD18mRNA的动态变化进行了研究。结果发现,在病毒感染后TLR2、TLR4、CD16mRNA的转录水平动态变化基本相似,3、7d高于对照组,14d低于对照组,28d接近对照组,且3d时TLR4、CD16差异极显著(P<0.01),14d仅TLR2差异显著(P<0.05);CD18mRNA的转录水平,3d时显著低于对照组(P<0.05),而7d时显著高于对照组(P<0.05),28d接近正常。以上结果表明,PCV2感染PAM后可引起PAM对异物识别吞噬功能与吞噬后信号转导功能出现紊乱,影响了PAM吞噬和清除病原微生物的能力。
