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Biomathematical study of time-dependent flow of a Carreau nanofluid through inclined catheterized arteries with overlapping stenosis 被引量:6
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作者 Ashfaq Ahmed Sohail Nadeem 《Journal of Central South University》 SCIE EI CAS CSCD 2017年第11期2725-2744,共20页
This work is concerned with the analysis of blood flow through inclined catheterized arteries having a balloon(angioplasty) with time-variant overlapping stenosis. The nature of blood in small arteries is analyzed mat... This work is concerned with the analysis of blood flow through inclined catheterized arteries having a balloon(angioplasty) with time-variant overlapping stenosis. The nature of blood in small arteries is analyzed mathematically by considering it as a Carreau nanofluid. The highly nonlinear momentum equations of nanofluid model are simplified by considering the mild stenosis case. The formulated problem is solved by a homotopy perturbation expansion in terms of a variant of the Weissenberg number to obtain explicit forms for the axial velocity, the stream function, the pressure gradient, the resistance impedance and the wall shear stress distribution. These solutions depend on the Brownian motion number, thermophoresis number, local temperature Grashof number G_r and local nanoparticle Grash of number B_r. The results were also studied for various values of the physical parameters, such as the Weissenberg number W_i, the power law index n, the taper angle φ, the maximum height of stenosis δ~*, the angle of inclination α, the maximum height of balloon σ~*, the axial displacement of the balloon z_d~*,the flow rate F and the Froud number Fr. The obtained results show that the transmission of axial velocity curves through a Newtonian fluid(Wi=0, n=1, Gr=0, Br=0, Nt=0, Nb≠0) is substantially lower than that through a Carreau nanofluid near the wall of balloon while the inverse occurs in the region between the balloon and stenosis. The streamlines have a clearly distinguished shifting toward the stenotic region and this shifting appears near the wall of the balloon, while it has almost disappeared near the stenotic wall and the trapping bolus in the case of horizontal arteries and Newtonian fluid(Wi=0, n=1, Gr=0, Br=0, Nt=0, Nb≠0) does not appear but for the case of Carreau nanofluid bolus appears. 展开更多
关键词 overlapping stenosed artery NANOFLUID Grashof number BROWNIAN motion THERMOPHORESIS pharmacology
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Automatic fuzzy-DBSCAN algorithm for morphological and overlapping datasets 被引量:5
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作者 YELGHI Aref KÖSE Cemal +1 位作者 YELGHI Asef SHAHKAR Amir 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2020年第6期1245-1253,共9页
Clustering is one of the unsupervised learning problems.It is a procedure which partitions data objects into groups.Many algorithms could not overcome the problems of morphology,overlapping and the large number of clu... Clustering is one of the unsupervised learning problems.It is a procedure which partitions data objects into groups.Many algorithms could not overcome the problems of morphology,overlapping and the large number of clusters at the same time.Many scientific communities have used the clustering algorithm from the perspective of density,which is one of the best methods in clustering.This study proposes a density-based spatial clustering of applications with noise(DBSCAN)algorithm based on the selected high-density areas by automatic fuzzy-DBSCAN(AFD)which works with the initialization of two parameters.AFD,by using fuzzy and DBSCAN features,is modeled by the selection of high-density areas and generates two parameters for merging and separating automatically.The two generated parameters provide a state of sub-cluster rules in the Cartesian coordinate system for the dataset.The model overcomes the problems of clustering such as morphology,overlapping,and the number of clusters in a dataset simultaneously.In the experiments,all algorithms are performed on eight data sets with 30 times of running.Three of them are related to overlapping real datasets and the rest are morphologic and synthetic datasets.It is demonstrated that the AFD algorithm outperforms other recently developed clustering algorithms. 展开更多
关键词 clustering density-based spatial clustering of applications with noise(DBSCAN) FUZZY overlapping data mining
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Bayesian method for system reliability assessment of overlapping pass/fail data 被引量:4
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作者 Zhipeng Hao Shengkui Zeng Jianbin Guo 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2015年第1期208-214,共7页
For high reliability and long life systems, system pass/fail data are often rare. Integrating lower-level data, such as data drawn from the subsystem or component pass/fail testing,the Bayesian analysis can improve th... For high reliability and long life systems, system pass/fail data are often rare. Integrating lower-level data, such as data drawn from the subsystem or component pass/fail testing,the Bayesian analysis can improve the precision of the system reliability assessment. If the multi-level pass/fail data are overlapping,one challenging problem for the Bayesian analysis is to develop a likelihood function. Since the computation burden of the existing methods makes them infeasible for multi-component systems, this paper proposes an improved Bayesian approach for the system reliability assessment in light of overlapping data. This approach includes three steps: fristly searching for feasible paths based on the binary decision diagram, then screening feasible points based on space partition and constraint decomposition, and finally simplifying the likelihood function. An example of a satellite rolling control system demonstrates the feasibility and the efficiency of the proposed approach. 展开更多
关键词 system reliability assessment Bayesian analysis limited samples overlapping pass/fail data
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Density-based rough set model for hesitant node clustering in overlapping community detection 被引量:2
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作者 Jun Wang Jiaxu Peng Ou Liu 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2014年第6期1089-1097,共9页
Overlapping community detection in a network is a challenging issue which attracts lots of attention in recent years.A notion of hesitant node(HN) is proposed. An HN contacts with multiple communities while the comm... Overlapping community detection in a network is a challenging issue which attracts lots of attention in recent years.A notion of hesitant node(HN) is proposed. An HN contacts with multiple communities while the communications are not strong or even accidental, thus the HN holds an implicit community structure.However, HNs are not rare in the real world network. It is important to identify them because they can be efficient hubs which form the overlapping portions of communities or simple attached nodes to some communities. Current approaches have difficulties in identifying and clustering HNs. A density-based rough set model(DBRSM) is proposed by combining the virtue of densitybased algorithms and rough set models. It incorporates the macro perspective of the community structure of the whole network and the micro perspective of the local information held by HNs, which would facilitate the further "growth" of HNs in community. We offer a theoretical support for this model from the point of strength of the trust path. The experiments on the real-world and synthetic datasets show the practical significance of analyzing and clustering the HNs based on DBRSM. Besides, the clustering based on DBRSM promotes the modularity optimization. 展开更多
关键词 density-based rough set model(DBRSM) overlapping community detection rough set hesitant node(HN) trust path
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A novel weighted evolving network model based on clique overlapping growth
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作者 杨旭华 王波 孙豹 《Journal of Central South University》 SCIE EI CAS 2010年第4期830-835,共6页
A novel weighted evolving network model based on the clique overlapping growth was proposed.The model shows different network characteristics under two different selection mechanisms that are preferential selection an... A novel weighted evolving network model based on the clique overlapping growth was proposed.The model shows different network characteristics under two different selection mechanisms that are preferential selection and random selection.On the basis of mean-field theory,this model under the two different selection mechanisms was analyzed.The analytic equations of distributions of the number of cliques that a vertex joins and the vertex strength of the model were given.It is proved that both distributions follow the scale-free power-law distribution in preferential selection mechanism and the exponential distribution in random selection mechanism,respectively.The analytic expressions of exponents of corresponding distributions were obtained.The agreement between the simulations and analytical results indicates the validity of the theoretical analysis.Finally,three real transport bus networks(BTNs) of Beijing,Shanghai and Hangzhou in China were studied.By analyzing their network properties,it is discovered that these real BTNs belong to a kind of weighted evolving network model with clique overlapping growth and random selection mechanism that was proposed in this context. 展开更多
关键词 weighted network clique overlapping mean-field theory bus transport network
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Overlap吻合与π吻合在全腹腔镜全胃切除术中的应用 被引量:1
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作者 李锋 彭德伟 +2 位作者 李文艺 何静 黄金向 《郑州大学学报(医学版)》 CAS 北大核心 2024年第4期531-536,共6页
目的:比较Overlap吻合与π吻合在全腹腔镜全胃切除术(TLTG)中的应用效果。方法:选择2019年2月至2022年2月在成都市第五人民医院行TLTG的胃癌患者98例,根据食管空肠吻合方式分为Overlap吻合组(n=56)和π吻合组(n=42)。比较两组围手术期指... 目的:比较Overlap吻合与π吻合在全腹腔镜全胃切除术(TLTG)中的应用效果。方法:选择2019年2月至2022年2月在成都市第五人民医院行TLTG的胃癌患者98例,根据食管空肠吻合方式分为Overlap吻合组(n=56)和π吻合组(n=42)。比较两组围手术期指标(手术时间、吻合时间、术中出血量、淋巴结清扫数目、术后肛门排气时间、术后拔除引流管时间、术后住院时间、肿瘤上缘至食管切缘距离),吻合口相关并发症(吻合口出血、吻合口狭窄、吻合口漏)与随访并发症(吻合口溃疡、倾倒综合征、反流性食管炎、营养不良)发生情况,以及术前与术后3个月整体营养状况主观评估量表(PG-SGA)评分与营养状况客观指标(血红蛋白、白蛋白、总蛋白)水平的变化。结果:Overlap吻合组手术时间[(230.36±25.12)min vs(205.58±20.43)min]、吻合时间[(43.22±6.15)min vs(27.45±5.36)min]均长于π吻合组(P<0.05);两组吻合口相关并发症、随访并发症发生率及术前、术后3个月PG-SGA量表评分与营养状况客观指标水平的变化差异均无统计学意义(P>0.05)。结论:Overlap吻合和π吻合在TLTG术中各有优势,π吻合可缩短手术时间和吻合时间。 展开更多
关键词 胃癌 全腹腔镜全胃切除术 Overlap吻合 π吻合 并发症 营养状况
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Novel method for extraction of ship target with overlaps in SAR image via EM algorithm 被引量:1
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作者 CAO Rui WANG Yong 《Journal of Systems Engineering and Electronics》 SCIE CSCD 2024年第4期874-887,共14页
The quality of synthetic aperture radar(SAR)image degrades in the case of multiple imaging projection planes(IPPs)and multiple overlapping ship targets,and then the performance of target classification and recognition... The quality of synthetic aperture radar(SAR)image degrades in the case of multiple imaging projection planes(IPPs)and multiple overlapping ship targets,and then the performance of target classification and recognition can be influenced.For addressing this issue,a method for extracting ship targets with overlaps via the expectation maximization(EM)algorithm is pro-posed.First,the scatterers of ship targets are obtained via the target detection technique.Then,the EM algorithm is applied to extract the scatterers of a single ship target with a single IPP.Afterwards,a novel image amplitude estimation approach is pro-posed,with which the radar image of a single target with a sin-gle IPP can be generated.The proposed method can accom-plish IPP selection and targets separation in the image domain,which can improve the image quality and reserve the target information most possibly.Results of simulated and real mea-sured data demonstrate the effectiveness of the proposed method. 展开更多
关键词 expectation maximization(EM)algorithm image processing imaging projection plane(IPP) overlapping ship tar-get synthetic aperture radar(SAR)
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Influence of sampling on three-dimensional surface shape measurement
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作者 QIAO Nao-sheng Shang Xue 《中国光学(中英文)》 EI CAS CSCD 北大核心 2024年第6期1512-1520,共9页
In order to accurately measure an object’s three-dimensional surface shape,the influence of sampling on it was studied.First,on the basis of deriving spectra expressions through the Fourier transform,the generation o... In order to accurately measure an object’s three-dimensional surface shape,the influence of sampling on it was studied.First,on the basis of deriving spectra expressions through the Fourier transform,the generation of CCD pixels was analyzed,and its expression was given.Then,based on the discrete expression of deformation fringes obtained after sampling,its Fourier spectrum expression was derived,resulting in an infinitely repeated"spectra island"in the frequency domain.Finally,on the basis of using a low-pass filter to remove high-order harmonic components and retaining only one fundamental frequency component,the inverse Fourier transform was used to reconstruct the signal strength.A method of reducing the sampling interval,i.e.,reducing the number of sampling points per fringe,was proposed to increase the ratio between the sampling frequency and the fundamental frequency of the grating.This was done to reconstruct the object’s surface shape more accurately under the condition of m>4.The basic principle was verified through simulation and experiment.In the simulation,the sampling intervals were 8 pixels,4 pixels,2 pixels,and 1 pixel,the maximum absolute error values obtained in the last three situations were 88.80%,38.38%,and 31.50%in the first situation,respectively,and the corresponding average absolute error values are 71.84%,43.27%,and 32.26%.It is demonstrated that the smaller the sampling interval,the better the recovery effect.Taking the same four sampling intervals in the experiment as in the simulation can also lead to the same conclusions.The simulated and experimental results show that reducing the sampling interval can improve the accuracy of object surface shape measurement and achieve better reconstruction results. 展开更多
关键词 three-dimensional surface shape measurement sampling interval spectra overlapping measurement accuracy
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鸡白介素18与鸡γ干扰素基因融合表达载体构建 被引量:5
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作者 李晓婷 宋佰芬 崔玉东 《黑龙江八一农垦大学学报》 2017年第1期49-53,82,共6页
为了研究新型细胞因子制剂,构建鸡白细胞介素18和鸡干扰素γ的基因融合表达载体。无菌提取鸡脾细胞的总RNA,以其为模板经过RT-PCR的方法分别扩增出Ch IL-18和Ch IFN-γ基因片段,并连接到p MDTM18-T克隆载体上,构建重组质粒p MDTM18-T-Ch... 为了研究新型细胞因子制剂,构建鸡白细胞介素18和鸡干扰素γ的基因融合表达载体。无菌提取鸡脾细胞的总RNA,以其为模板经过RT-PCR的方法分别扩增出Ch IL-18和Ch IFN-γ基因片段,并连接到p MDTM18-T克隆载体上,构建重组质粒p MDTM18-T-Ch IL-18和p MDTM18-T-Ch IFN-γ。以重组质粒p MDTM18-T-Ch IL-18和p MDTM18-T-Ch IFN-γ为模板,利用多肽氨基酸接头和overlap技术,获得Ch IL-18-Ch IFN-γ融合基因并连接到克隆载体p MDTM18-T上,经PCR、酶切及测序鉴定正确后连接到p ET32a原核表达载体中。构建Ch IL-18-Ch IFN-γ原核表达载体。为下一步研究二者之间的作用关系及抗病毒功能奠定了基础。 展开更多
关键词 鸡白细胞介素18 鸡干扰素γ OVERLAP 原核表达载体
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关于基因重组中OLC算法的改进研究 被引量:1
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作者 买阿丽 杨雯雯 《郑州大学学报(理学版)》 CAS 北大核心 2016年第2期34-39,46,共7页
针对基因组组装问题,从数据预处理,利用KMP算法在O(m+n)的时间上快速确定某两个碱基片段的最大重复度,将读长序列依据Overlap图连成Contigs链以及Contigs N50的确定4个环节,改进现有的OLC拼接技术,并给出优化后的模型和算法,较好地解决... 针对基因组组装问题,从数据预处理,利用KMP算法在O(m+n)的时间上快速确定某两个碱基片段的最大重复度,将读长序列依据Overlap图连成Contigs链以及Contigs N50的确定4个环节,改进现有的OLC拼接技术,并给出优化后的模型和算法,较好地解决了基因组组装问题. 展开更多
关键词 基因重组 KMP算法 Overlap图 Contigs链 Contigs N50
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工业控制中多串口Overlapped IO通信的关键技术
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作者 李超 李运华 曹泽莼 《制造技术与机床》 CSCD 北大核心 2007年第12期90-94,共5页
在网络化数控系统和生产线中,串口通信技术是主控计算机(IPC)和节点设备之间广泛应用的通信技术之一。文章深入研究了IPC端NT环境下多串口的Overlapped IO方式工作特点,并详细给出了用Win32 API编写Overlapped模式下串口读取数据、发送... 在网络化数控系统和生产线中,串口通信技术是主控计算机(IPC)和节点设备之间广泛应用的通信技术之一。文章深入研究了IPC端NT环境下多串口的Overlapped IO方式工作特点,并详细给出了用Win32 API编写Overlapped模式下串口读取数据、发送数据以及多串口逻辑控制的程序框架,其实现方法为需要多串口通信应用的工业场合以及相关的研究工作提供了良好的工具平台。 展开更多
关键词 串口通信 Overlapped IO 数控系统
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IFCEM based recognition method for target with interval-overlapped hybrid attributes
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作者 GUAN Xin LI Shuangming +1 位作者 SUN Guidong WANG Haibin 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2023年第2期408-421,共14页
When the attributes of unknown targets are not just numerical attributes,but hybrid attributes containing linguistic attributes,the existing recognition methods are not effective.In addition,it is more difficult to id... When the attributes of unknown targets are not just numerical attributes,but hybrid attributes containing linguistic attributes,the existing recognition methods are not effective.In addition,it is more difficult to identify the unknown targets densely distributed in the feature space,especially when there is interval overlap between attribute measurements of different target classes.To address these problems,a novel method based on intuitionistic fuzzy comprehensive evaluation model(IFCEM)is proposed.For numerical attributes,targets in the database are divided into individual classes and overlapping classes,and for linguistic attributes,continuous interval-valued linguistic term set(CIVLTS)is used to describe target characteristic.A cloud modelbased method and an area-based method are proposed to obtain intuitionistic fuzzy decision information of query target on numerical attributes and linguistic attributes respectively.An improved inverse weighted kernel fuzzy c-means(IWK-FCM)algorithm is proposed for solution of attribute weight vector.The possibility matrix is applied to determine the identity and category of query target.Finally,a case study composed of parameter sensitivity analysis,recognition accuracy analysis.and comparison with other methods,is taken to verify the superiority of the proposed method. 展开更多
关键词 intuitionistic fuzzy comprehensive evaluation model(IFCEM) interval overlapping cloud model area-based method inverse weighted kernel fuzzy c-means(IWK-FCM)
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全腹腔镜全胃切除术中食管空肠overlap吻合术安全性和有效性的Meta分析 被引量:3
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作者 黄宗圆 曾爱明 +2 位作者 刘森 梁树芬 闫慧明 《海南医学院学报》 CAS 2023年第8期603-611,共9页
目的:系统评价和比较全腹腔镜全胃切除术中overlap食管空肠吻合术和传统食管空肠吻合术治疗胃癌的安全性和有效性,为临床实践提供循证医学证据。方法:通过计算机检索国内外数据库PubMed、Embase、Cochrane Library、Web of Science、知... 目的:系统评价和比较全腹腔镜全胃切除术中overlap食管空肠吻合术和传统食管空肠吻合术治疗胃癌的安全性和有效性,为临床实践提供循证医学证据。方法:通过计算机检索国内外数据库PubMed、Embase、Cochrane Library、Web of Science、知网、万方、维普,检索时限为各大数据库建库至2022年1月。获得相关文献,逐步筛选,纳入符合条件的研究,采用Cochrane ROB偏倚风险评估工具和NOS量表对文章进行质量评价,提取高质量文献中所需数据,最后通过Review Manager 5.3软件进行Meta分析。结果:最终纳入11项研究,包括1 398例患者,其中有566例接受overlap吻合术的患者和832例接受传统吻合术的患者。结果表明,overlap吻合术和传统吻合术在手术时间(MD=0.63,95%CI=7.22,8.48,P=0.88)、术后首次排气时间(MD=-0.13,95%CI=0.43,0.18,P=0.42)、术后首次进食时间(MD=0.02,95%CI=0.33,0.37,P=0.91)、吻合口漏(OR=1.38,95%CI=0.73,2.63,P=0.32)、术后住院时间(MD=-0.16,95%CI=0.82,0.51,P=0.64)方面无显著差异(均P>0.05)。与传统吻合术相比,overlap吻合术吻合时间较长(MD=5.60,95%CI=0.59,10.62,P=0.03)、吻合口出血发生率较高(OR=2.48,95%CI=1.08,5.69,P=0.03)、术中出血量较少(MD=-6.42,95%CI=10.28,-2.56,P=0.001)、吻合口狭窄发生率较低(OR=0.17,95%CI=0.06,0.46,P=0.000 6)。结论:overlap食管空肠吻合术是一种安全、有效、预后佳的手术方式,有望成为全腹腔镜全胃切除术中食管空肠吻合标准术式。 展开更多
关键词 胃癌 全腹腔镜全胃切除术 食管空肠吻合术 overlap吻合术 META分析
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黄曲霉寡聚-1,6-葡萄糖苷酶基因的克隆及其在工业酿酒酵母CICC1346中的表达 被引量:1
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作者 康小龙 吕晓静 +4 位作者 黄清媚 熊春江 林蒋海 肖文娟 刘泽寰 《食品与发酵工业》 CAS CSCD 北大核心 2016年第8期44-50,共7页
通过重叠延伸PCR(gene splicing by overlap extension,SOE-PCR)扩增黄曲霉寡聚-1,6-葡萄糖苷酶基因和酿酒酵母α-信号肽序列,定向重组到整合型表达载体pδRCMB中,并在CICC1346中实现分泌表达;然后通过同源建模、利用分子模拟软件Discov... 通过重叠延伸PCR(gene splicing by overlap extension,SOE-PCR)扩增黄曲霉寡聚-1,6-葡萄糖苷酶基因和酿酒酵母α-信号肽序列,定向重组到整合型表达载体pδRCMB中,并在CICC1346中实现分泌表达;然后通过同源建模、利用分子模拟软件Discovery Studio 4.1分析其蛋白结构,以对硝基苯-α-D-葡萄糖吡喃苷(pNPG)为底物,建立并确定酶活反应条件,并进行纯化、酶学性质分析;将密码子优化后序列在CICC1346中实现分泌表达,利用淀粉进行共发酵实验。结果显示:重组酶突变后相对野生型蛋白结构无影响。SDS-PAGE分析重组重组酶大小约为70 kDa;优化后最高酶活达到0.69 U/m L,重组酶最适pH为6.5,在pH4.5.0~7.0维持90%以上的酶活;最适温度为40℃,在30~40℃维持接近100%的酶活;受Cu^(2+)和Mn^(2+)严格抑制;寡聚-1,6-葡萄糖苷酶与α-淀粉酶及糖化酶协同利用淀粉产乙醇,提高淀粉水解效率。这是首次报道黄曲霉的寡聚-1,6-葡萄糖苷酶基因在酿酒酵母整合型分泌表达。 展开更多
关键词 酿酒酵母 黄曲霉 重叠延伸PCR(splicing by OVERLAP extension SOE—PCR) 同源建模 寡聚-1 6-葡萄糖 苷酶 淀粉
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多项Probit模型参数的极大似然估计 被引量:1
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作者 赵江涛 徐兴忠 《应用数学》 CSCD 北大核心 2004年第S2期90-93,共4页
本文考虑多项probit模型中参数的极大似然估计(MLE)的存在性.在协方差阵已知和均匀结构两种情况下,给出MLE存在的充要条件.
关键词 多项Probit模型 极大似然估计 OVERLAP
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Overlapped OFDMA——新颖的多载波频谱共享传输机制
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作者 宫本康 张朝阳 叶露 《浙江大学学报(工学版)》 EI CAS CSCD 北大核心 2013年第5期860-866,共7页
针对现有非连续正交频分复用(DOFDM)等系统通常只允许认知用户利用授权用户未使用的频带进行共享传输的问题,提出一种新颖的频谱共享传输机制,即混叠正交频分复用多址接入(Overlapped OFDMA),能够在一定条件下实现认知用户与授权用户的... 针对现有非连续正交频分复用(DOFDM)等系统通常只允许认知用户利用授权用户未使用的频带进行共享传输的问题,提出一种新颖的频谱共享传输机制,即混叠正交频分复用多址接入(Overlapped OFDMA),能够在一定条件下实现认知用户与授权用户的混叠频谱共享.通过对一般OFDM系统的信号结构进行研究发现,当认知用户的子载波带宽采用授权用户子载波带宽的一半进行传输、其频谱和授权用户频谱存在一定混叠时,只要认知用户保持和授权用户时域信号准同步且其子载波分配方案满足一定的限制条件,授权用户将不受干扰.由于各载波上的干扰都包含有其它载波的信息,因此认知用户可以通过适当的恢复算法将信息完整地恢复.仿真和分析结果表明,尽管Overlapped OFDMA会带来一定的复杂度,但授权用户的性能和DOFDM系统相比几乎相同,而认知用户却获得了更加优异的吞吐量性能.该机制为频谱共享传输体制的设计提供了新的可能性. 展开更多
关键词 Overlapped OFDMA 认知无线电 频谱共享传输
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全腹腔镜全胃切除术食管空肠吻合术反穿刺法与Overlap法的比较 被引量:15
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作者 汪兵 夏亚斌 +1 位作者 张义胜 赵军 《中国微创外科杂志》 CSCD 北大核心 2020年第7期590-594,共5页
目的比较反穿刺法与Overlap法在全腹腔镜全胃切除术(totally laparoscopic total gastrectomy,TLTG)食管空肠吻合中的应用效果。方法回顾性分析我院2017年1月~2018年12月75例TLTG的临床资料,依据食管空肠重建方式不同,分为反穿刺组(n=41... 目的比较反穿刺法与Overlap法在全腹腔镜全胃切除术(totally laparoscopic total gastrectomy,TLTG)食管空肠吻合中的应用效果。方法回顾性分析我院2017年1月~2018年12月75例TLTG的临床资料,依据食管空肠重建方式不同,分为反穿刺组(n=41)和Overlap组(n=34)。结果75例均顺利完成全TLTG。与Overlap组比较,反穿刺组吻合时间[(46.4±6.3)min vs(52.9±4.6)min,t=-5.028,P=0.000]和术后住院时间[(11.0±1.3)d vs(11.6±1.2)d,t=-2.363,P=0.021]明显缩短,住院费用明显减少[(44720.7±3499.2)元vs.(48164.6±5536.5)元,t=-3.274,P=0.002],但肠道恢复时间显著延长[(3.8±1.1)d vs.(3.2±0.6)d,t=2.675,P=0.009]。2组总手术时间、术中出血量、清扫淋巴结数目差异无统计学意义(P>0.05)。结论全腹腔镜下食管空肠吻合术管型吻合的反穿刺法和线型吻合的Overlap法均安全可行,但反穿刺法在吻合时间和手术费用上更有优势。 展开更多
关键词 反穿刺法 Overlap吻合 腹腔镜 全胃切除术
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腹腔镜左半结肠切除术中结肠结肠侧侧吻合(Overlap)的临床价值 被引量:9
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作者 钟克力 夏利刚 +4 位作者 胡海军 陈杰 李明伟 林烈文 郭春华 《中国微创外科杂志》 CSCD 北大核心 2019年第11期996-999,共4页
目的探讨腹腔镜左半结肠切除术中结肠结肠侧侧吻合(Overlap)的临床应用价值。方法2014年5月~2017年10月,按患者及家属意愿将53例腹腔镜下左半结肠根治术分为腔内吻合组(19例)和腔外吻合组(34例)。腔内吻合组采用直线切割缝合器镜下完成O... 目的探讨腹腔镜左半结肠切除术中结肠结肠侧侧吻合(Overlap)的临床应用价值。方法2014年5月~2017年10月,按患者及家属意愿将53例腹腔镜下左半结肠根治术分为腔内吻合组(19例)和腔外吻合组(34例)。腔内吻合组采用直线切割缝合器镜下完成Overlap吻合,扩大脐部观察孔至4 cm将标本和标本袋取出;腔外吻合组扩大左侧腹切口至4~6 cm,体外完成切除和结肠结肠端侧吻合。比较2组手术时间、术中出血量、术后24 h疼痛评分、肛门排气时间、术后住院时间、并发症和手术费用。结果腔内吻合组术后疼痛评分低[(5.6±1.4)分vs.(6.7±1.5)分,t=-2.508,P=0.015],但手术费用高[(2.8±0.4)万元vs.(2.1±0.4)万元,t=5.952,P=0.000],2组手术时间、术中出血量、排气时间、术后住院时间、并发症无显著性差异(P>0.05)。结论腹腔镜左半结肠切除术采用Overlap吻合方式安全可行,更为微创。 展开更多
关键词 腹腔镜 左半结肠切除术 Overlap吻合
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山羊痘病毒SS株5个ANK基因缺失的重组病毒构建 被引量:2
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作者 杨勇飞 张成 +3 位作者 张雪萍 童剑军 高娜 李有文 《现代畜牧兽医》 2021年第1期58-62,共5页
为研究锚定蛋白基因(Ankyrin,ANK)对山羊痘病毒的影响,试验采用融合PCR和Overlap PCR技术扩增山羊痘病毒SS株5个ANK基因(ANK010、ANK138、ANK140、ANK141.2和ANK145)两端侧翼序列和绿色荧光蛋白(GFP)基因,并将其产物连接Trans1-T 1载体... 为研究锚定蛋白基因(Ankyrin,ANK)对山羊痘病毒的影响,试验采用融合PCR和Overlap PCR技术扩增山羊痘病毒SS株5个ANK基因(ANK010、ANK138、ANK140、ANK141.2和ANK145)两端侧翼序列和绿色荧光蛋白(GFP)基因,并将其产物连接Trans1-T 1载体构建ANK缺失的转移载体,经过菌液PCR以及质粒双酶切鉴定,阳性重组质粒用Lip 2000转染至已经感染羊痘病毒SS株的羊睾丸原代细胞,依报告基因GFP的表达情况在荧光显微镜下筛选目的基因缺失的重组病毒,同时设立不感染病毒的对照。结果表明:通过融合PCR方法成功扩增山羊痘病毒SS株ANK基因010和138的两端侧翼序列及GFP基因片段,大小约1200 bp;通过Overlap PCR方法成功得到ANK基因140、141.2和145基因的侧翼及GFP基因片段,大小约900 bp,与理论相符。研究成功构建了基因缺失转移载体,将其转染感染SS病毒的细胞中,5个ANK基因缺失的表达载体均可见绿色荧光斑点,说明得到各自基因缺失的重组羊痘病毒。 展开更多
关键词 羊痘病毒 GFP 基因缺失 融合PCR 重组病毒 Overlap PCR
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多元秩-序模型MLE的存在性
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作者 孙立敏 徐兴忠 《应用数学》 CSCD 北大核心 2004年第S2期115-119,共5页
本文对多元秩 序模型极大似然估计的存在性进行了研究,在对模型协方差阵Ω的一些约束下,文中给出了其参数极大似然估计存在的一些充分必要条件.
关键词 多元秩-序模型 极大似然估计(MLE) OVERLAP
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