OBJECTIVE To explore the therapeutic effect and its mechanism of persimmon leaf flavonoids(PLF) on rats with oral ulcer. METHODS The oral ulcer model was induced by acetic acid,was used for intervention of Guilin wate...OBJECTIVE To explore the therapeutic effect and its mechanism of persimmon leaf flavonoids(PLF) on rats with oral ulcer. METHODS The oral ulcer model was induced by acetic acid,was used for intervention of Guilin watermelon frost and different dose(20,40 and 80 mg·kg^(-1)) of PLF.Ulcer area was calculated on the fourth and the seventh day after injury; the changes of superoxide dismutase(SOD) and malondialdehyde(MDA) in serum and ulcer tissues were observed; the level of tumor necrosis factor alpha(TNF-alpha) in ulcer tissue was measured. To observe the pathological morphological changes of H-E staining. RESULTS Guilin watermelon frost and PLF(40 and 80 mg·kg^(-1))can reduce the ulcer area(P<0.05); PLF(20,40 and 80 mg·kg^(-1)) can increase the activity of SOD and decrease the content of MDA in serum and ulcer tissue(P<0.05); Guilin watermelon frost and PLF could significantly decrease the levels of TNF-alpha in ulcer tissue(P<0.05),and improve the inflammatory infiltration of ulcer tissue. CONCLUSION PLF has certain therapeutic effects on oral ulcer induced by acetic acid,and the mechanism may be related to improve oxidative damage and reduce inflammatory reaction.展开更多
目的探讨加味葛根芩连汤治疗复发性口腔溃疡(Recurrent oral ulceration,ROU)疗效及对免疫功能的影响。方法选取ROU患者112例,按1∶1简单随机法分为观察组与对照组,各56例。对照组予以康复新液,观察组予以加味葛根芩连汤,均持续治疗8周...目的探讨加味葛根芩连汤治疗复发性口腔溃疡(Recurrent oral ulceration,ROU)疗效及对免疫功能的影响。方法选取ROU患者112例,按1∶1简单随机法分为观察组与对照组,各56例。对照组予以康复新液,观察组予以加味葛根芩连汤,均持续治疗8周。记录两组疗效、口腔溃疡数量,评估两组溃疡疼痛[视觉模拟评分(Visual analogue scale,VAS)]、中医证候积分、外周血炎性细胞因子[白细胞介素(Interleukin,IL)-1β、IL-17、IL-10、肿瘤坏死因子(Tumor necrosis factor,TNF-α)]水平、细胞免疫功能(CD3^(+)、CD4^(+)、CD8^(+))水平、生存质量[世界卫生组织生存质量测定量表(World health organization quality of life scale,WHOQOL-100)]及不良反应。于治疗后随访8周评估复发率。结果相较于对照组,观察组总有效率更高(P<0.05)。治疗后观察组中医证候积分和溃疡数量低(少)于对照组(P<0.05)。与治疗前对比,两组治疗4、8周后的血清IL-1β、IL-17、IL-10和TNF-α水平均降低,且观察组均低于对照组,其中治疗8周后观察组血清指标最低(P<0.05)。与治疗前对比,两组治疗4、8周后的CD3^(+)、CD4^(+)水平均升高,CD8^(+)水平均降低,且观察组CD3^(+)、CD4^(+)水平均高于对照组,CD8^(+)水平低于对照组,其中治疗8周后观察组CD3^(+)、CD4^(+)水平最高,CD8^(+)水平最低(P<0.05)。随治疗时间的变化,两组VAS评分逐渐下降,WHOQOL-100评分逐渐升高,且治疗2、4、6、8周后观察组VAS评分低于对照组,WHOQOL-100评分高于对照组(P<0.05)。两组不良反应发生率对比差异无统计学意义(P>0.05)。观察组复发率较对照组更低(P<0.05)。结论加味葛根芩连汤治疗ROU不仅利于提升患者临床疗效,减轻炎症反应和疼痛程度,还可提高免疫功能和生存质量,降低复发率,且安全性也较高。展开更多
基金The project supported by National Undergraduate Training Program for Innovation and Entrepreneurship(201510439015)
文摘OBJECTIVE To explore the therapeutic effect and its mechanism of persimmon leaf flavonoids(PLF) on rats with oral ulcer. METHODS The oral ulcer model was induced by acetic acid,was used for intervention of Guilin watermelon frost and different dose(20,40 and 80 mg·kg^(-1)) of PLF.Ulcer area was calculated on the fourth and the seventh day after injury; the changes of superoxide dismutase(SOD) and malondialdehyde(MDA) in serum and ulcer tissues were observed; the level of tumor necrosis factor alpha(TNF-alpha) in ulcer tissue was measured. To observe the pathological morphological changes of H-E staining. RESULTS Guilin watermelon frost and PLF(40 and 80 mg·kg^(-1))can reduce the ulcer area(P<0.05); PLF(20,40 and 80 mg·kg^(-1)) can increase the activity of SOD and decrease the content of MDA in serum and ulcer tissue(P<0.05); Guilin watermelon frost and PLF could significantly decrease the levels of TNF-alpha in ulcer tissue(P<0.05),and improve the inflammatory infiltration of ulcer tissue. CONCLUSION PLF has certain therapeutic effects on oral ulcer induced by acetic acid,and the mechanism may be related to improve oxidative damage and reduce inflammatory reaction.
文摘目的探讨加味葛根芩连汤治疗复发性口腔溃疡(Recurrent oral ulceration,ROU)疗效及对免疫功能的影响。方法选取ROU患者112例,按1∶1简单随机法分为观察组与对照组,各56例。对照组予以康复新液,观察组予以加味葛根芩连汤,均持续治疗8周。记录两组疗效、口腔溃疡数量,评估两组溃疡疼痛[视觉模拟评分(Visual analogue scale,VAS)]、中医证候积分、外周血炎性细胞因子[白细胞介素(Interleukin,IL)-1β、IL-17、IL-10、肿瘤坏死因子(Tumor necrosis factor,TNF-α)]水平、细胞免疫功能(CD3^(+)、CD4^(+)、CD8^(+))水平、生存质量[世界卫生组织生存质量测定量表(World health organization quality of life scale,WHOQOL-100)]及不良反应。于治疗后随访8周评估复发率。结果相较于对照组,观察组总有效率更高(P<0.05)。治疗后观察组中医证候积分和溃疡数量低(少)于对照组(P<0.05)。与治疗前对比,两组治疗4、8周后的血清IL-1β、IL-17、IL-10和TNF-α水平均降低,且观察组均低于对照组,其中治疗8周后观察组血清指标最低(P<0.05)。与治疗前对比,两组治疗4、8周后的CD3^(+)、CD4^(+)水平均升高,CD8^(+)水平均降低,且观察组CD3^(+)、CD4^(+)水平均高于对照组,CD8^(+)水平低于对照组,其中治疗8周后观察组CD3^(+)、CD4^(+)水平最高,CD8^(+)水平最低(P<0.05)。随治疗时间的变化,两组VAS评分逐渐下降,WHOQOL-100评分逐渐升高,且治疗2、4、6、8周后观察组VAS评分低于对照组,WHOQOL-100评分高于对照组(P<0.05)。两组不良反应发生率对比差异无统计学意义(P>0.05)。观察组复发率较对照组更低(P<0.05)。结论加味葛根芩连汤治疗ROU不仅利于提升患者临床疗效,减轻炎症反应和疼痛程度,还可提高免疫功能和生存质量,降低复发率,且安全性也较高。
