Through computer screening of Genbank and experimental analysis a novel snoRNA, termed Z25, was first identified from mammals. Z25 snoRNA is 69 nucleotide in length, possessing typical boxC/D and terminal repeat seque...Through computer screening of Genbank and experimental analysis a novel snoRNA, termed Z25, was first identified from mammals. Z25 snoRNA is 69 nucleotide in length, possessing typical boxC/D and terminal repeat sequence, a 11 nt long sequence complementarity to 18S rRNA implies that Z25 snoRNA is a 2′ O ribose methylation guide at A1678 (human coordinate) of 18S rRNA. Z25 snoRNA is encoded by the fifth intron of human, mouse and rat nucleolin genes that have been found as the host genes for U20 and U23 snoRNA.展开更多
目的:探讨腹主动脉缩窄致压力负荷增加大鼠心肌肥厚模型中核仁素的表达情况.方法:采用体质量180～220 g SD大鼠40只,随机分为假手术组和腹主动脉缩窄模型组,利用腹主动脉缩窄法制备压力负荷增加心肌肥厚模型,分别于术后2周、4周观察心...目的:探讨腹主动脉缩窄致压力负荷增加大鼠心肌肥厚模型中核仁素的表达情况.方法:采用体质量180～220 g SD大鼠40只,随机分为假手术组和腹主动脉缩窄模型组,利用腹主动脉缩窄法制备压力负荷增加心肌肥厚模型,分别于术后2周、4周观察心脏质量指数、左心室质量指数;采用RT-PCR检测心肌组织中β-MHC mRNA的表达;采用Western印迹检测心肌、脑、肾组织中核仁素的表达情况.结果:腹主动脉缩窄模型组4周以后心脏质量指数、左心室质量指数较假手术组显著增加(P＜0.01);4周以后心肌组织中β-MHC mRNA的表达较假手术组显著升高(P＜0.05);2周以后心肌组织中核仁素蛋白的表达较假手术组显著升高(P＜0.05),而在脑、肾组织中无明显升高.结论:核仁素蛋白在大鼠压力负荷增加心肌肥厚模型中的表达上调,表明核仁素可能参与了压力负荷增加心肌肥厚的发生发展.展开更多
文摘Through computer screening of Genbank and experimental analysis a novel snoRNA, termed Z25, was first identified from mammals. Z25 snoRNA is 69 nucleotide in length, possessing typical boxC/D and terminal repeat sequence, a 11 nt long sequence complementarity to 18S rRNA implies that Z25 snoRNA is a 2′ O ribose methylation guide at A1678 (human coordinate) of 18S rRNA. Z25 snoRNA is encoded by the fifth intron of human, mouse and rat nucleolin genes that have been found as the host genes for U20 and U23 snoRNA.
文摘目的:探讨腹主动脉缩窄致压力负荷增加大鼠心肌肥厚模型中核仁素的表达情况.方法:采用体质量180～220 g SD大鼠40只,随机分为假手术组和腹主动脉缩窄模型组,利用腹主动脉缩窄法制备压力负荷增加心肌肥厚模型,分别于术后2周、4周观察心脏质量指数、左心室质量指数;采用RT-PCR检测心肌组织中β-MHC mRNA的表达;采用Western印迹检测心肌、脑、肾组织中核仁素的表达情况.结果:腹主动脉缩窄模型组4周以后心脏质量指数、左心室质量指数较假手术组显著增加(P＜0.01);4周以后心肌组织中β-MHC mRNA的表达较假手术组显著升高(P＜0.05);2周以后心肌组织中核仁素蛋白的表达较假手术组显著升高(P＜0.05),而在脑、肾组织中无明显升高.结论:核仁素蛋白在大鼠压力负荷增加心肌肥厚模型中的表达上调,表明核仁素可能参与了压力负荷增加心肌肥厚的发生发展.
