A total of 59 isolates of Aeromonas hydrophila were collected from common carp suffering from freshwater fish hemorrhage disease in 13 fishing grounds in the northeast China, and their phenotypic and genetic character...A total of 59 isolates of Aeromonas hydrophila were collected from common carp suffering from freshwater fish hemorrhage disease in 13 fishing grounds in the northeast China, and their phenotypic and genetic characteristics were investigated. All of the isolates were identified as A. hydrophila by traditional biochemical method and yielded a 686-bp DNA fragment of the 16S rDNA gene in the PCR experiments. Collected strains were also evaluated for their susceptibility to 17 different antibiotics. The isolates showed an even trend of the resistance and sensitivity to drugs, highly sensitive to antibiotics, such as Levofloxacin, PolymyxinB, Ofloxacin and resistant to antibiotics, such as Bristopen, Lincomycin, Ampicillin, Teicoplanin. Evaluation of genetic diversity was performed on all isolates by molecular typing with enterobacterial repetitive intergenic consensus （ERIC-PCR） method. The results showed that three different types, i.e. type I, ]I, and type ~I, were found in 59 isolates and type III accounted for a large proportion of 54.84%. There was no dominant difference between the tendency of the isolates of Heilongjiang Province and Jilin Province in these three types, which showed DI 〉 I 〉 I, while the isolates of Liaoning Province showed III 〉 II 〉 I. The percentage of different types in different provinces varied in each other; however, they didn＇t show any obvious regional or cluster-specific branches. In conclusion, the ability to distinguish Aeromonas hydrophila strains from diseased common carp with ERIC-PCR would be useful for epidemiological investigation and population genetic analysis of this pathogen in China.展开更多
Based on global monthly average data set of sea surface temperature (SST) during 1950 - 1992 and global monthly average 500 hPa height during 1930- 1997 offered by NCARINCEP, the feature of SST anomaly in the Atlantic...Based on global monthly average data set of sea surface temperature (SST) during 1950 - 1992 and global monthly average 500 hPa height during 1930- 1997 offered by NCARINCEP, the feature of SST anomaly in the Atlantic and its relation with summer precipitation over the Northeast China are analyzed. The results show that, the second eigenvector of the SST’s empirical orthogonal expanssion in winter season over the North Atlantic suggests that dist-ibution of SST anomaly has unusual meridional difference; The location of its center is basically identical to center of significant correlation region be- tween summer precipitation over the Northeast China and winter SST in the Atlantic. When winter SST in the North Atlantic is hot in south and cold in north, the blocking situation is stronger in the middle- high latitude. Correspondingly, the blocking high pressure in the northern North Pacific is also getting stronger, the westerlies circulation index in East Asia in next summer would be lower,as a result,more precipitation in the summer would be experienced over Northeast China and vice versa.展开更多
基金Supported by the Central-Level Non-profit Scientific Research Institutes Special Funds (201104)the Natural Science Foundation of Heilongjiang Province of China (C201140)
文摘A total of 59 isolates of Aeromonas hydrophila were collected from common carp suffering from freshwater fish hemorrhage disease in 13 fishing grounds in the northeast China, and their phenotypic and genetic characteristics were investigated. All of the isolates were identified as A. hydrophila by traditional biochemical method and yielded a 686-bp DNA fragment of the 16S rDNA gene in the PCR experiments. Collected strains were also evaluated for their susceptibility to 17 different antibiotics. The isolates showed an even trend of the resistance and sensitivity to drugs, highly sensitive to antibiotics, such as Levofloxacin, PolymyxinB, Ofloxacin and resistant to antibiotics, such as Bristopen, Lincomycin, Ampicillin, Teicoplanin. Evaluation of genetic diversity was performed on all isolates by molecular typing with enterobacterial repetitive intergenic consensus （ERIC-PCR） method. The results showed that three different types, i.e. type I, ]I, and type ~I, were found in 59 isolates and type III accounted for a large proportion of 54.84%. There was no dominant difference between the tendency of the isolates of Heilongjiang Province and Jilin Province in these three types, which showed DI 〉 I 〉 I, while the isolates of Liaoning Province showed III 〉 II 〉 I. The percentage of different types in different provinces varied in each other; however, they didn＇t show any obvious regional or cluster-specific branches. In conclusion, the ability to distinguish Aeromonas hydrophila strains from diseased common carp with ERIC-PCR would be useful for epidemiological investigation and population genetic analysis of this pathogen in China.
文摘Based on global monthly average data set of sea surface temperature (SST) during 1950 - 1992 and global monthly average 500 hPa height during 1930- 1997 offered by NCARINCEP, the feature of SST anomaly in the Atlantic and its relation with summer precipitation over the Northeast China are analyzed. The results show that, the second eigenvector of the SST’s empirical orthogonal expanssion in winter season over the North Atlantic suggests that dist-ibution of SST anomaly has unusual meridional difference; The location of its center is basically identical to center of significant correlation region be- tween summer precipitation over the Northeast China and winter SST in the Atlantic. When winter SST in the North Atlantic is hot in south and cold in north, the blocking situation is stronger in the middle- high latitude. Correspondingly, the blocking high pressure in the northern North Pacific is also getting stronger, the westerlies circulation index in East Asia in next summer would be lower,as a result,more precipitation in the summer would be experienced over Northeast China and vice versa.
