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Effect of sterilization methods on quality and storage characteristics of tofu fermented by lactic acid bacteria
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作者 Yang Liu Huifang Liu +3 位作者 Jiangting Hao Xueting Li Liang Li Xiaoyu Yang 《Grain & Oil Science and Technology》 2025年第1期32-42,共11页
This study examined the effects of pasteurization(PAS),ultrasonic sterilization(ULS),and microwave sterilization(MWS)on the quality and storage characteristics of brine-fermented tofu(BFT)and fermented tofu(FT).Compar... This study examined the effects of pasteurization(PAS),ultrasonic sterilization(ULS),and microwave sterilization(MWS)on the quality and storage characteristics of brine-fermented tofu(BFT)and fermented tofu(FT).Comparative analysis revealed that MWS had a negligible detrimental effect on the structural integrity and organoleptic properties of BFT and FT,while effectively maintaining its water-holding capacity(WHC)and exhibiting the least impact on its texture.In contrast,PAS and ULS increased hardness and chewiness significantly(P<0.05),but ULS also enhanced the brightness of tofu.Throughout the storage period,the WHC,elasticity,and sensory properties of tofu generally decreased,whereas the hardness and chewiness increased.PAS-BFT and MWS-FT maintained sensory quality for the longest periods of 14 and 12 days respectively,and could be decomposed to more small molecule peptides within 0–8 days and 0–6 days,which are more easily to be absorbed by the body.The findings discovered that MWS is the most suitable method for sterilization of tofu,with superior capability in maintaining the quality,extending shelf life,and improving digestibility of tofu. 展开更多
关键词 lactic acid bacteria Fermented tofu STERILIZATION QUALITY Storage characteristics
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Lactic acid bacteria act as potent interventions in improving hyperuricemia:a review
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作者 Qingling Wu Xiaodong Pei +2 位作者 Tiantian Gao Xiaoling Liu Chenghua Wang 《Food Science and Human Wellness》 2025年第4期1212-1223,共12页
Hyperuricemia(HUA)is characterized by elevated levels of uric acid(UA)in the bloodstream,resulting from either excessive production or insufficient excretion of UA within the body.If left untreated,progressive or pers... Hyperuricemia(HUA)is characterized by elevated levels of uric acid(UA)in the bloodstream,resulting from either excessive production or insufficient excretion of UA within the body.If left untreated,progressive or persistent HUA can lead to gout,causing significant harm to human health.Lactic acid bacteria(LAB),generally recognized as safe(GRAS)probiotics,have been shown to alleviate symptoms associated with gastrointestinal disorders such as irritable bowel syndrome and inflammatory bowel disease while supporting overall bodily functions and health.Recently,LAB has emerged as a potentially safe,cost-effective and efficient treatment for HUA.This comprehensive review aims to explore the current literature on the mechanisms through which LAB controls HUA.These mechanisms include suppressing purine metabolism,absorbing purine compounds,modulating microbiota to maintain host global purine homeostasis,reducing intestinal permeability,producing metabolites that alleviate HUA symptoms,promoting the expression of urate excretory proteins and inhibiting the expression of urate reabsorption proteins.The findings presented in this review provide a framework for further investigation into how probiotic LAB can alleviate HUA by influencing UA metabolism and elucidating their underlying action mechanisms. 展开更多
关键词 HYPERURICEMIA GOUT lactic acid bacteria Uric acid-lowering mechanism
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Preparation of lactic acid bacteria compound starter cultures based on pasting properties and its improvement of glutinous rice flour and dough 被引量:1
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作者 Dengyu Wang Linlin Liu +4 位作者 Bing Wang Wenjian Xie Yanguo Shi Na Zhang Hongchen Fan 《Food Science and Human Wellness》 SCIE CAS CSCD 2024年第4期2090-2101,共12页
The effects of 5 lactic acid bacteria(LAB)fermentation on the pasting properties of glutinous rice flour were compared,and suitable fermentation strains were selected based on the changes of viscosity,setback value,an... The effects of 5 lactic acid bacteria(LAB)fermentation on the pasting properties of glutinous rice flour were compared,and suitable fermentation strains were selected based on the changes of viscosity,setback value,and breakdown value to prepare LAB compound starter cultures.The results revealed that Latilactobacillus sakei HSD004 and Lacticaseibacillus rhamnosus HSD005 had apparent advantages in increasing the viscosity and reducing the setback and breakdown values of glutinous rice flour.In particular,the compound starter created using the two abovementioned LAB in the ratio of 3:1 had better performance than that using a single LAB in improving the pasting properties and increasing the water and oil absorption capacity of glutinous rice flour.Moreover,the gelatinization enthalpy of the fermented samples increased significantly.For frozen glutinous rice dough stored for 28 days,the viscoelasticity of frozen dough prepared by compound starter was better than that of control dough,and the freezable water content was lower than that of control dough.These results indicate that compound LAB fermentation is a promising technology in the glutinous rice-based food processing industry,which has significance for its application. 展开更多
关键词 Glutinous rice flour Glutinous rice dough lactic acid bacteria compound starter cultures Pasting properties VISCOELASTICITY
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Co-inoculation of Debaryomyces hansenii and lactic acid bacteria: a strategy to improve the taste and odour profiles of dry sausages
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作者 Rongxin Wen Yumeng Sui +4 位作者 Jiaqi Liu Huiping Wang Baohua Kong Ligang Qin Qian Chen 《Food Science and Human Wellness》 SCIE CAS CSCD 2024年第6期3273-3283,共11页
The effects of the co-inoculation of Debaryomyces hansenii separately with 3 lactic acid bacteria(LAB),Lactobacillus sakei,Lactobacillus plantarum and Lactobacillus curvatus,on the taste and odour profi les of dry sau... The effects of the co-inoculation of Debaryomyces hansenii separately with 3 lactic acid bacteria(LAB),Lactobacillus sakei,Lactobacillus plantarum and Lactobacillus curvatus,on the taste and odour profi les of dry sausages were investigated.The co-inoculated sausages showed higher free amino acid and organic acid contents than the non-inoculated control and sausages inoculated with D.hansenii alone.Meanwhile,the sausages inoculated with D.hansenii+L.plantarum,D.hansenii+L.sakei and D.hansenii+L.curvatus had the highest contents of aldehydes,esters and alcohols,respectively.The results of electronic tongue,electronic nose and sensory evaluation demonstrated that compared with the sausage inoculated with D.hansenii,the sour taste and fl oral odour increased and the fatty odour decreased in the sausage inoculated with D.hansenii+L.sakei;this was more favourable for the development of a desirable fl avour in sausages.Moreover,the partial least squares regression analysis indicated that 10 taste and 33 odour compounds were mainly responsible for the differences in the flavour profiles among the sausages.Overall,these findings contributed to a more comprehensive understanding of the formation of sensory characteristics in dry sausages co-inoculated with yeast and LAB. 展开更多
关键词 Debaryomyces hansenii lactic acid bacteria Dry sausage CO-INOCULATION Flavour profile
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Allergen degradation of bee pollen by lactic acid bacteria fermentation and its alleviatory effects on allergic reactions in BALB/c mice
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作者 Shuting Yin Qiangqiang Li +5 位作者 Yuxiao Tao Enning Zhou Kun Wang Wanwen Chen Xiangxin Li Liming Wu 《Food Science and Human Wellness》 SCIE CSCD 2024年第1期349-359,共11页
Food allergy as a global health problem threatens food industry.Bee pollen(BP)is a typical food with allergenic potentials,although it performs various nutritional/pharmacological functions to humans.In this study,lac... Food allergy as a global health problem threatens food industry.Bee pollen(BP)is a typical food with allergenic potentials,although it performs various nutritional/pharmacological functions to humans.In this study,lactic acid bacteria(LAB)were used to ferment Brassica napus BP for alleviating its allergenicity.Four novel allergens(glutaredoxin,oleosin-B2,catalase and lipase)were identified with significant decreases in LAB-fermented BP(FBP)than natural BP by proteomics.Meanwhile,metabolomics analysis showed significant increases of 28 characteristic oligopeptides and amino acids in FBP versus BP,indicating the degradation of LAB on allergens.Moreover,FBP showed alleviatory effects in BALB/c mice,which relieved pathological symptoms and lowered production of allergic mediators.Microbial high-throughput sequencing analysis showed that FBP could regulate gut microbiota and metabolism to strengthen immunity,which were closely correlated with the alleviation of allergic reactivity.These findings could contribute to the development and utilization of hypoallergenic BP products. 展开更多
关键词 Alleviatory effects lactic acid bacteria fermentation Bee pollen allergen Gut microbiota Host metabolism
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Screening of glucosinolates degrading lactic acid bacteria and their utilization in rapeseed meal fermentation
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作者 Yifang Chen Chong Xie +2 位作者 Muhammad Bilal Pei Wang Runqiang Yang 《Grain & Oil Science and Technology》 CAS 2024年第3期168-176,共9页
Rapeseed meal is a promising food ingredient, but its utilization is limited by the presence of some potentially harmful ingredients, such as glucosinolates. Fermentation is a cost-effective method of detoxication but... Rapeseed meal is a promising food ingredient, but its utilization is limited by the presence of some potentially harmful ingredients, such as glucosinolates. Fermentation is a cost-effective method of detoxication but a food-grade starter culture with glucosinolates degradation capacity is required. In this study, 46 strains of lactic acid bacteria from traditional paocai brines were screened for their ability to glucosinolate degradation. The results showed that more than 50% of the strains significantly degraded glucosinolates. Two strains of Lactiplantibacillus(p7 and s7) with high capacity of glucosinolates degradation through producing enzymes were identified. Then,an optimized condition for rapeseed meal fermentation by p7 was established to degrade glucosinolates, which can achieve about 80% degradation. UPLC/Q-TOF-MS analysis showed that the degradation rate of individual glucosinolates was different and the degradation rate of gluconapin and progoitrin in rapeseed meal can reach more than 90%. Meanwhile, fermentation with p7 can improve safety of rapeseed meal by inhibiting the growth of Enterobacteriaceae and improve its nutritional properties by degrading phytic acid. The in vitro digestion experiments showed that the content of glucosinolates in rapeseed meal decreased significantly during gastric digestion. Meanwhile, fermentation with p7 can greatly improve the release of soluble protein and increase the contents of free essential amino acids, such as lysine(increased by 12 folds) and methionine(increased by 10 folds). 展开更多
关键词 Rapeseed meal GLUCOSINOLATES FERMENTATION lactic acid bacteria In vitro digestion
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3D printed lactic acid bacteria hydrogel:cell release kinetics and stability 被引量:1
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作者 Yifei Liu Xintao Yin +3 位作者 Xiudong Xia Zhen Liu Lifei Chen Mingsheng Dong 《Food Science and Human Wellness》 SCIE CSCD 2023年第2期477-487,共11页
In this study,a new type of 3 D printed living biological hydrogel was developed by integrating lactic acid bacteria(LAB)into biocompatible and non-toxic polymer materials.Interestingly,the living materials loaded wit... In this study,a new type of 3 D printed living biological hydrogel was developed by integrating lactic acid bacteria(LAB)into biocompatible and non-toxic polymer materials.Interestingly,the living materials loaded with LAB can be freeze-dried and reused for more than 100 times.The bio-hydrogel can be used to co-culture different LAB and keep its fermentation performance stable in long-term use.The release kinetics model and response surface method were used to simulate and optimize the bacteria release mode in the bio-hydrogel.The results show that the release of bacteria from hydrogel is regulated by the coupling of Fickian diffusion and polymer swelling.The stability of LAB hydrogel was evaluated by reuse experiments.The images of confocal microscopy and scanning electron microscope showed that the bacteria with high cell viability were distributed in the hydrogel and intact structure of the living hydrogel was maintained after 100 times of reuse as yoghurt starter.In conclusion,the 3 D printed LAB bio-hydrogel developed in this study has the advantage of reuse and sustainability,which is expected to open up a new way for the preparation of food culture starter. 展开更多
关键词 3D printing Bioink Living hydrogel lactic acid bacteria Culture starter
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燕麦β-葡聚糖对凝固型酸奶发酵特性及贮藏品质的影响
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作者 李真 张钰 +3 位作者 蔡杰 杨勇 索标 艾志录 《轻工学报》 北大核心 2025年第2期23-30,共8页
在不同质量分数的燕麦β-葡聚糖(Oatβ-glucan,OBG)水溶液中加入脱脂奶粉制备凝固型酸奶,并测定其pH值、酸度、流变特性、持水力(WHC)、乳酸菌活菌数等,考查OBG对凝固型酸奶发酵特性及贮藏品质的影响。结果表明:与对照组相比,OBG对发酵... 在不同质量分数的燕麦β-葡聚糖(Oatβ-glucan,OBG)水溶液中加入脱脂奶粉制备凝固型酸奶,并测定其pH值、酸度、流变特性、持水力(WHC)、乳酸菌活菌数等,考查OBG对凝固型酸奶发酵特性及贮藏品质的影响。结果表明:与对照组相比,OBG对发酵期间的pH值无显著性影响(P>0.05),但会显著降低发酵后期(6~8 h)和后熟24 h酸奶的酸度(P<0.05),且显著增加后熟24 h酸奶的WHC(P<0.05)。添加质量分数为0.4%的OBG可使后熟24 h酸奶表现出更高的储能模量(G′)和损耗模量(G″),即黏弹性较佳,感官品质也较优;随着贮藏时间的延长,实验组和对照组的乳酸菌活菌数均呈先升高后降低的趋势,且当贮藏15 d时,0.4%OBG组的乳酸菌活菌数最多(9.12 log CFU/mL)。因此,适量添加OBG不仅能增强凝固型酸奶的发酵特性,还能显著提升其贮藏品质,并对凝固型酸奶中的乳酸菌起到保护作用。 展开更多
关键词 燕麦Β-葡聚糖 乳酸菌 凝固型酸奶 发酵特性 贮藏品质
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乳酸菌接种发酵生产辣椒酱的工艺优化
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作者 万景瑞 蒋鹏飞 +5 位作者 史冠莹 肖作兵 张乐 赵丽丽 赵妍 王赵改 《中国食品添加剂》 2025年第2期84-92,共9页
为解决传统自然发酵辣椒酱生产条件不受控制、产品品质不稳定的问题,以期通过接种不同乳酸菌和优化发酵工艺生产一款高品质的辣椒酱。本研究以感官评价、理化指标(总酸、辣椒红色素、亚硝酸盐含量)为评价指标,采用对照、单因素和正交试... 为解决传统自然发酵辣椒酱生产条件不受控制、产品品质不稳定的问题,以期通过接种不同乳酸菌和优化发酵工艺生产一款高品质的辣椒酱。本研究以感官评价、理化指标(总酸、辣椒红色素、亚硝酸盐含量)为评价指标,采用对照、单因素和正交试验优化辣椒酱最佳工艺条件。结果表明,影响发酵辣椒酱产品品质的因素主次顺序为菌种接种量>蔗糖添加量>食盐添加量,辣椒酱的最佳发酵工艺条件:乳酸菌三菌为最适菌种,接种量2.0%、大蒜添加量8%、蔗糖添加量7%、食盐添加量9%、发酵时间15 d。利用此工艺生产的辣椒酱整体品质最优,产品鲜亮有光泽,呈亮红色,组织细腻均匀,发酵香味浓郁,酸咸味适度、辣味突出,产品的理化指标、微生物指标符合国家标准。 展开更多
关键词 乳酸菌 接种发酵 辣椒酱 工艺优化
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瑞士乳杆菌与耐酸乳杆菌复合强化对液态发酵食醋风味的影响
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作者 黄浩伦 陆震鸣 +5 位作者 柴丽娟 张晓娟 许泓瑜 李信 史劲松 许正宏 《食品与发酵工业》 CAS 北大核心 2025年第1期143-150,共8页
该研究将瑞士乳杆菌(Lactobacillus helveticus)与耐酸乳杆菌(Lactobacillus acetotolerans)分阶段接种于液态发酵食醋的发酵过程中,以提升食醋品质。采用高效液相色谱、顶空固相微萃取-气质联用和电子舌等分析方法,对液态发酵食醋的有... 该研究将瑞士乳杆菌(Lactobacillus helveticus)与耐酸乳杆菌(Lactobacillus acetotolerans)分阶段接种于液态发酵食醋的发酵过程中,以提升食醋品质。采用高效液相色谱、顶空固相微萃取-气质联用和电子舌等分析方法,对液态发酵食醋的有机酸含量、挥发性化合物含量进行分析并对其进行感官评价。结果表明,L.helveticus L-6与L.acetotolerans L-9复合强化后,液态发酵食醋总酸可达5.15 g/100 mL;还原糖残留量为3.41 g/L;乳酸、丙酮酸含量提升显著,其中乳酸含量提升幅度最大,可达12.8 g/L;复合强化可以显著提升乙酸乙酯、乳酸乙酯、苯乙酸等具有花果香、奶香和甜香风味的挥发性化合物含量;感官评价结果表明,两种乳酸菌分阶段复合强化发酵的食醋甜味和鲜味增加且酸味明显减弱,风味品质明显提升。该研究证实了在液态发酵食醋的发酵过程中进行L.helveticus L-6与L.acetotolerans L-9复合强化,有助于优化食醋有机酸组成,提升食醋的风味品质,为食醋的酿造工艺优化和产品创新提供参考。 展开更多
关键词 乳酸菌 液态发酵食醋 生物强化 有机酸 风味
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内蒙古地区自然发酵乳中乳酸菌的分离鉴定及优良菌株筛选
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作者 雷雪艳 赵丽霞 +1 位作者 李瑜 刘文俊 《食品与发酵工业》 CAS 北大核心 2025年第1期11-17,共7页
鲜乳及其发酵乳是牧民日常饮食中主要的营养物来源之一,其中含有丰富的乳酸菌资源。为了从纯培养角度了解不同乳源中乳酸菌的菌群结构并获取其中宝贵的乳酸菌菌种,该研究采用纯培养技术从内蒙古牧区21份样品中分离得到411株乳酸菌,包括1... 鲜乳及其发酵乳是牧民日常饮食中主要的营养物来源之一,其中含有丰富的乳酸菌资源。为了从纯培养角度了解不同乳源中乳酸菌的菌群结构并获取其中宝贵的乳酸菌菌种,该研究采用纯培养技术从内蒙古牧区21份样品中分离得到411株乳酸菌,包括10个属,21个种。其中乳酸乳球菌(Lactococcus lactis)分离株的数量最多,其次为瑞士乳杆菌(Lactobacillus helveticus)和马乳酒样乳杆菌(Lactobacillus kefiranofaciens)。优势菌群分析发现不同乳源的菌群结构差异较大,酸马乳的菌群多样性最丰富,马乳酒样乳杆菌是其优势菌种之一。根据酸马乳低pH值、低酒精含量的特点,从93株马乳酒样乳杆菌中筛选出4株在低pH值(pH 3.0)和6%(体积分数)乙醇环境下耐受性较好的菌株,然后进一步研究其人工胃肠液耐受性、乙醇耐受性和对3种食品中常见致病菌的抑菌能力。最终筛选出2株马乳酒样乳杆菌分别为:IMAU12331与IMAU12208,具有较好的耐受性和抑菌特性,可作为潜在益生菌开发利用,具有较高的潜在应用价值。 展开更多
关键词 乳酸菌 分离鉴定 菌株筛选 耐受性 抑菌特性
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乳酸菌发酵对米乳品质、风味和抗氧化活性的影响
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作者 高小妹 隋勇 +6 位作者 祝振洲 李书艺 周雷 施建斌 熊添 蔡芳 梅新 《食品工业科技》 北大核心 2025年第4期127-135,共9页
为了探究乳酸菌发酵对米乳品质、风味及抗氧化活性的影响,本研究以黑米、红米和糙米为原料,以乳粉和蔗糖为辅料,添加直投式乳酸菌发酵剂制成发酵型米乳。并以相同条件下不添加发酵剂制成的非发酵型米乳为对照,比较分析6种米乳的稳定性... 为了探究乳酸菌发酵对米乳品质、风味及抗氧化活性的影响,本研究以黑米、红米和糙米为原料,以乳粉和蔗糖为辅料,添加直投式乳酸菌发酵剂制成发酵型米乳。并以相同条件下不添加发酵剂制成的非发酵型米乳为对照,比较分析6种米乳的稳定性、蛋白质含量、还原糖含量、总酚含量、DPPH和ABTS+自由基清除活性以及挥发性风味物质的差异。结果显示,乳酸菌发酵显著(P<0.05)提高了米乳的酸度、蛋白质含量、总酚含量、抗氧化活性,降低了米乳的pH、可溶性固形物和还原糖含量,对离心沉淀率无显著影响。气相色谱-质谱联用仪和电子鼻分析结果显示,与对照组相比,乳酸菌发酵后米乳中风味物质的种类和含量显著提高,正己醇、苯甲醛等米乳的主要香气成分含量分别增加了0.23%~2.42%、0.11%~0.72%。乳酸菌发酵后的米乳具有特有的发酵风味。研究结果可为深入认识乳酸菌发酵对米乳品质、风味和抗氧化活性的影响及发酵谷物饮料的开发提供一定的理论依据。 展开更多
关键词 米乳 乳酸菌发酵 营养 风味 抗氧化活性
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肠膜明串珠菌MG12的抑菌特性及其对西梅采后品质的影响
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作者 张芯语 叶艺洁 +3 位作者 木尼热·木塔力甫 楚敏 朱静 张志东 《食品与发酵工业》 北大核心 2025年第6期175-182,192,共9页
西梅采后生理代谢旺盛,贮藏过程中果实易失水萎蔫、腐败变质。乳酸菌可代替化学防腐剂应用于果蔬的贮藏保鲜,既能起到抑菌作用,又能保持果蔬品质。为探明肠膜明串株菌发酵液对采后西梅贮藏保鲜效果,该研究解析了肠膜明串株菌MG12发酵液... 西梅采后生理代谢旺盛,贮藏过程中果实易失水萎蔫、腐败变质。乳酸菌可代替化学防腐剂应用于果蔬的贮藏保鲜,既能起到抑菌作用,又能保持果蔬品质。为探明肠膜明串株菌发酵液对采后西梅贮藏保鲜效果,该研究解析了肠膜明串株菌MG12发酵液的抑菌性能,并采用发酵液浸泡西梅,评价了处理后的西梅贮藏品质。结果表明,MG12发酵液能有效抑制狭卵链格孢菌(Alternaria angustiovoidea)和大肠埃希菌(Escherichia coli),并且经不同温度、pH、酶和紫外辐照处理后仍保留较好的抑菌活性。使用发酵上清液处理(处理组)的西梅在放置15 d后发病率比对照组低37.03%、失重率比对照组低5.17%、可溶性固形物含量比对照组高5.73%、可滴定酸含量比对照组高11%,处理组硬度是对照组的2.28倍。肠膜明串株菌MG12发酵液处理表现出较好的西梅采后贮藏保鲜效果。 展开更多
关键词 乳酸菌 肠膜明串株菌 西梅贮藏 抑菌剂
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植物乳植杆菌IMAUJBP3和罗伊氏乳杆菌IMAUJBR3复配对发酵羊肉香肠品质及多肽抗氧化能力的影响
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作者 夏玲燕 钱敏 +5 位作者 徐晔 程峰 王待巽 张开屏 田建军 靳烨 《食品科学》 北大核心 2025年第6期80-88,共9页
为探究植物乳植杆菌IMAUJBP3和罗伊氏乳杆菌IMAUJBR3复配对发酵香肠品质及多肽抗氧化能力的影响,将实验室前期制备的具有优良特性的乳酸菌接种到发酵羊肉香肠中,分析了香肠的品质指标、风味及多肽含量和抗氧化活性。结果表明复配乳酸菌... 为探究植物乳植杆菌IMAUJBP3和罗伊氏乳杆菌IMAUJBR3复配对发酵香肠品质及多肽抗氧化能力的影响,将实验室前期制备的具有优良特性的乳酸菌接种到发酵羊肉香肠中,分析了香肠的品质指标、风味及多肽含量和抗氧化活性。结果表明复配乳酸菌可以快速降低发酵香肠的pH值、水分活度和水分含量;同时丁酸乙酯、戊酸乙酯、芳樟醇、反式-2-壬醛等24种物质(相对气味活度值>1)对风味起主导作用;复配乳酸菌显著提升了发酵香肠多肽含量以及抗氧化活性(P<0.05)。综上所述,植物乳植杆菌IMAUJBP3与罗伊氏乳杆菌IMAUJBR3复配在一定程度上改善发酵羊肉香肠的品质、风味和多肽抗氧化活性。 展开更多
关键词 乳酸菌 复配作用 发酵羊肉香肠 风味 多肽 抗氧化
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乳酸菌发酵诱导水蜜桃浆凝胶体系自发变色的4D打印研究
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作者 王岩 牛犇 +5 位作者 王冠楠 房祥军 刘瑞玲 吴伟杰 陈慧芝 郜海燕 《食品与发酵工业》 北大核心 2025年第8期143-150,共8页
为拓宽食品的4D打印品类,推动食品4D打印技术发展,将水蜜桃浆与马铃薯粉、黄原胶、海藻酸钠复配成适合打印的凝胶体系,添加天然花青素和乳酸菌构建4D打印凝胶体系,研究乳酸菌发酵诱导的水蜜桃浆凝胶体系自发变色和品质变化。结果表明,... 为拓宽食品的4D打印品类,推动食品4D打印技术发展,将水蜜桃浆与马铃薯粉、黄原胶、海藻酸钠复配成适合打印的凝胶体系,添加天然花青素和乳酸菌构建4D打印凝胶体系,研究乳酸菌发酵诱导的水蜜桃浆凝胶体系自发变色和品质变化。结果表明,当马铃薯粉的添加量为25%(质量分数)时,打印凝胶体系具有良好的流动性和支撑性能;随着发酵时间的延长,4D打印凝胶体系的乳酸菌数量大量增长、霉菌生长受到一定抑制;乳酸菌的生长引起打印物pH值降低约1.8,导致打印物中花青素的颜色从紫色变为红色,发酵12 h的ΔE值为15.2;此外,打印物的质构特性(如硬度、弹性、胶着性和回复性)随发酵时间的延长而出现小幅度增加,这与发酵过程中乳酸菌数量的动态变化和水分的变化(水分质量分数降低5.2%)相关。 展开更多
关键词 水蜜桃浆凝胶 4D打印 乳酸菌发酵 自发变色 花青素
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乳酸菌对不同比例王草和百香果果皮混合青贮发酵品质和干物质消化率的影响
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作者 吕仁龙 蒋睿珂 +4 位作者 张雨书 蒋博 李茂 周汉林 高玲 《中国饲料》 北大核心 2025年第5期125-131,共7页
为研究添加乳酸菌对王草与百香果果皮不同比例混合青贮发酵品质和干物质消化率的影响,试验将王草与百香果果皮混合青贮比例分别设为10:0(0%)、9:1(10%)、8:2(20%)、7:3(30%)、6:4(40%)、5:5(50%),再分别做无添加和乳酸菌(1.33×10^(... 为研究添加乳酸菌对王草与百香果果皮不同比例混合青贮发酵品质和干物质消化率的影响,试验将王草与百香果果皮混合青贮比例分别设为10:0(0%)、9:1(10%)、8:2(20%)、7:3(30%)、6:4(40%)、5:5(50%),再分别做无添加和乳酸菌(1.33×10^(5) CFU/g)添加组(n=3),利用体外培养方法分析干物质消化率和瘤胃液发酵参数。结果表明:添加乳酸菌后,青贮pH显著下降(P<0.01),乳酸含量显著升高(P<0.01),挥发性氨态氮含量和乙酸含量显著降低(P<0.05),体外培养后的产气量和干物质消化率相比无添加组也显著提高(P<0.05)。随着百香果皮添加量的增加,青贮pH显著降低(P<0.05),乳酸含量显著上升(P<0.01),在50%添加比例下达到最高。在瘤胃液发酵方面,随着百香果皮添加量增加,pH逐渐下降,产气和干物质消化率逐渐上升(P<0.05),在50%添加组达到最高。综上所述,乳酸菌、百香果果皮均有利于提升混合青贮发酵品质、营养价值和干物质消化率,结合实际生产,王草与百香果果皮混合青贮下,百香果果皮最大且最适添加比例为40%~50%。 展开更多
关键词 百香果皮 王草 青贮 乳酸菌 干物质消化率
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芸豆-蓝靛果复合发酵液制备工艺优化及其抗氧化活性
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作者 王佳 丁方莉 +9 位作者 安宇 曾雪莹 张智慧 李思楠 徐开媛 周芳 王颖 张璐 徐炳政 孙泽堃 《食品工业科技》 北大核心 2025年第3期222-231,共10页
为提高杂粮与小浆果的利用度,研究复配乳酸菌发酵芸豆-蓝靛果复合汁制备发酵液并优化其工艺参数。选取四种乳酸菌对芸豆-蓝靛果复合汁接种发酵,以活菌数为指标筛选复配乳酸菌后,进行单因素实验及响应面优化试验确定复合发酵液的工艺参数... 为提高杂粮与小浆果的利用度,研究复配乳酸菌发酵芸豆-蓝靛果复合汁制备发酵液并优化其工艺参数。选取四种乳酸菌对芸豆-蓝靛果复合汁接种发酵,以活菌数为指标筛选复配乳酸菌后,进行单因素实验及响应面优化试验确定复合发酵液的工艺参数,并对其理化性质、生物活性及抗氧化能力进行检测。结果表明,筛选得到复配乳酸菌L1:L2等比例复配接种芸豆-蓝靛果复合汁,优化得到的复合发酵液的最优工艺参数为原料复配比例1:1、初始pH5.0、接种量2%、发酵温度34℃,此条件下复合发酵液的活菌数为9.89 lg CFU/mL。经检测,复合发酵液中总酚及黄酮含量均在16 h达到最高,分别为2204.96μg/mL和701.09μg/mL。抗氧化实验表明,在发酵过程中芸豆-蓝靛果复合汁的DPPH自由基清除率及ABTS+自由基清除率呈现先上升后缓慢下降的趋势,最高清除值为98.23%和93.77%,最优条件制备的芸豆-蓝靛果复合发酵液抗氧化能力较强。 展开更多
关键词 芸豆 蓝靛果 乳酸菌 工艺优化 抗氧化活性
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乳酸菌发酵对燕麦面团及馒头品质特性的影响
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作者 李佳豪 孙冰华 +1 位作者 钱晓洁 王晓曦 《粮食与油脂》 北大核心 2025年第1期31-37,共7页
为了改善燕麦粉加工的适应性,选用植物乳杆菌(Lactobacillus plantarum HH-LP56)分别对燕麦生粉和不同糊化度(13%、17%、24%、30%、34%)燕麦熟粉进行发酵改性,探究乳酸菌发酵对燕麦粉的游离氨基酸、淀粉酶活性、还原糖含量和热特性,以... 为了改善燕麦粉加工的适应性,选用植物乳杆菌(Lactobacillus plantarum HH-LP56)分别对燕麦生粉和不同糊化度(13%、17%、24%、30%、34%)燕麦熟粉进行发酵改性,探究乳酸菌发酵对燕麦粉的游离氨基酸、淀粉酶活性、还原糖含量和热特性,以及燕麦-小麦复配面团的流变发酵特性和馒头质构特性的影响。结果表明:乳酸菌发酵促进了燕麦生粉中游离氨基酸的释放,增加了燕麦生粉和熟粉中淀粉酶活性和还原糖含量。将发酵改性燕麦生/熟粉与小麦粉进行复配,改善了面团的发酵特性,制得的馒头比体积上升,硬度下降,馒头的品质特性得到有效改善。 展开更多
关键词 乳酸菌 燕麦粉 糊化度 加工性能
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乳酸菌细菌素及其对金黄色葡萄球菌生物膜抑制作用研究进展
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作者 薛小格 刘园园 +5 位作者 张丽纳 樊睿 辛宇 马佳歌 于微 刘丽波 《食品工业科技》 北大核心 2025年第4期414-424,共11页
金黄色葡萄球菌(Staphylococcus aureus,S.aureus)是一种能在食品表面形成生物膜的革兰氏阳性条件致病菌,其生物膜的形成对食品加工和食品安全造成了严重威胁。乳酸菌细菌素是由乳酸菌在代谢过程中产生的一类具有抗菌活性的多肽,可抑制... 金黄色葡萄球菌(Staphylococcus aureus,S.aureus)是一种能在食品表面形成生物膜的革兰氏阳性条件致病菌,其生物膜的形成对食品加工和食品安全造成了严重威胁。乳酸菌细菌素是由乳酸菌在代谢过程中产生的一类具有抗菌活性的多肽,可抑制多种食源性致病菌及其生物膜的形成。本文针对乳酸菌细菌素的分类、作用方式及其在群体感应调控下的合成机制进行了综述,基于培养条件和培养基成分简述了提高乳酸菌细菌素合成量的常规方法,总结了不同来源乳酸菌细菌素的纯化方式,系统综述了乳酸菌细菌素对S.aureus生物膜的抑制作用,从Agr系统和LuxS/AI-2群体感应系统的角度简要阐述了乳酸菌细菌素对S.aureus生物膜的调控机制,并展望了乳酸菌细菌素对S.aureus生物膜抑制机制的未来研究方向,为乳酸菌细菌素作为生物防腐剂的开发和广泛使用提供科学依据。 展开更多
关键词 乳酸菌细菌素 金黄色葡萄球菌 生物膜 抑菌机制 分离纯化
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基于模糊数学感官评价-TOPSIS法的花生粕发酵乳优良乳酸菌筛选
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作者 陆开祥 陈中爱 +2 位作者 刘丽静 薛桥丽 胡永金 《中国调味品》 北大核心 2025年第3期76-83,共8页
为促进花生粕的综合利用,以13株乳酸菌发酵花生粕乳,采用逼近理想解排序(TOPSIS)法分别从酸度、黏度、氨基酸态氮浓度、持水力、活菌数和感官属性等方面进行综合评价,探究乳酸菌在花生粕乳中的发酵性能。结果表明,植物乳杆菌植物亚种(L6... 为促进花生粕的综合利用,以13株乳酸菌发酵花生粕乳,采用逼近理想解排序(TOPSIS)法分别从酸度、黏度、氨基酸态氮浓度、持水力、活菌数和感官属性等方面进行综合评价,探究乳酸菌在花生粕乳中的发酵性能。结果表明,植物乳杆菌植物亚种(L6)的产酸速率最高,达到9.57°T/h,其次是植物乳杆菌(X2),为9.26°T/h。植物乳杆菌(X2)和副干酪乳杆菌(X5)的后酸化能力较弱,贮藏期间酸度变化量分别为10.84°T和9.58°T,且这两株菌产氨基酸态氮浓度较高,分别为589.87μg/mL和562.28μg/mL。嗜热链球菌(S2)的产黏能力和持水力最强,分别为1227.33 mPa·s和42.62%,且其综合感官评分最高,为89.75分。发酵乳杆菌(FYa1)和植物乳杆菌(MDb2)的活菌数最高,分别为8.89 lg CFU/mL和8.80 lg CFU/mL。采用TOPSIS法综合评价,得出嗜热链球菌(S2)为综合发酵性能较优良的菌株,以其为核心菌株辅以乳酸杆菌作为发酵剂应用于花生粕乳的发酵,可为花生粕乳发酵产品的开发提供有效路径。 展开更多
关键词 花生粕乳 乳酸菌 模糊数学 TOPSIS法
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