Background and objective Low-density computed tomography(LDCT)improved early lung cancer diagnosis but introduces an excess of false-positive pulmonary nodules data.Hence,accurate diagnosis of early-stage lung cancer ...Background and objective Low-density computed tomography(LDCT)improved early lung cancer diagnosis but introduces an excess of false-positive pulmonary nodules data.Hence,accurate diagnosis of early-stage lung cancer remains challenging.The purpose of the study was to assess the feasibility of using circulating tumour cells(CTCs)to differentiate malignant from benign pulmonary nodules.Materials and methods 122 patients with suspected malignant pulmonary nodules detected on chest CT in preparation for surgery were prospectively recruited.Peripheral blood samples were collected before surgery,and CTCs were identified upon isolation by size of epithelial tumour cells and morphological analysis.Laser capture microdissection,MALBAC amplification,and whole-exome sequencing were performed on 8 samples.The diagnostic efficacy of CTCs counting,and the genomic variation profile of benign and malignant CTCs samples were analysed.Results Using 2.5 cells/5 m L as the cut-off value,the area under the receiver operating characteristic curve was of 0.651(95%confidence interval:0.538-0.764),with a sensitivity and specificity of 0.526 and 0.800,respectively,and positive and negative predictive values of 91.1%and 30.3%,respectively.Distinct sequence variations differences in DNA damage repair-related and driver genes were observed in benign and malignant samples.TP53 mutations were identified in CTCs of four malignant cases;in particular,g.7578115T>C,g.7578645C>T,and g.7579472G>C were exclusively detected in all four malignant samples.Conclusion CTCs play an ancillary role in the diagnosis of pulmonary nodules.TP53 mutations in CTCs might be used to identify benign and malignant pulmonary nodules.展开更多
Viral-mediated gene transfer of thymidine kinase ofherpes simplex virus (HSV-tk) has been used to confercytotoxic sensitivity to ganciclovir (GCV) in a variety oftnmor cells. HSV-tk converts GCV into a phosphorylatedc...Viral-mediated gene transfer of thymidine kinase ofherpes simplex virus (HSV-tk) has been used to confercytotoxic sensitivity to ganciclovir (GCV) in a variety oftnmor cells. HSV-tk converts GCV into a phosphorylatedcompound which is toxic for dividing cells by blockingDNA synthesis. Our previous study has shown展开更多
In order to investigate the characteristics of particle-induced pressure loss in the solid–liquid lifting pipe,a series of experiments were conducted in 200 mm diameter lifting pipe.Simulation manganese nodules with ...In order to investigate the characteristics of particle-induced pressure loss in the solid–liquid lifting pipe,a series of experiments were conducted in 200 mm diameter lifting pipe.Simulation manganese nodules with five different mean diameters of10 mm,20 mm,30 mm,40 mm and 50 mm were used,both in isolation and a combination in equal fraction by mass.The flow velocities in the lifting pipe ranged from 0.12 m/s to 1.61 m/s,and the mass of particles employed was 10 kg for each particle diameter.Three regimes,wavy bed,partly fluidization,and fully fluidization,were observed over the flow velocity.The solid–liquid pressure drop data were measured by differential pressure transmitter,and pressure drop caused by the solid particles was calculated and analyzed.The results show that the evolutions of the pressure loss due to solid particles are relevant to the solid–liquid flow regimes,and they are distinctly influenced by fluid velocity and particle size.展开更多
Marine geology is one of the important mainstays in marine sciences.Since themiddle of this century,due to inumerable systematic investigations,especially the appli-cation of advanced science and technology of various...Marine geology is one of the important mainstays in marine sciences.Since themiddle of this century,due to inumerable systematic investigations,especially the appli-cation of advanced science and technology of various kinds,there has emerged a series ofimportant discoveries in the study of marine geology.These discoveries,while makingcertain traditional basic concepts and theories of geology questionable,led to the birth ofa number of new theories.For example,the discovery of changes in the paleooceanic en-展开更多
To make sure that the nodule collector can walk on the soft sediments of seafloor effectively, suspension principle of deep-ocean nodule collector is proposed. The supporting and driving mechanisms are separated to av...To make sure that the nodule collector can walk on the soft sediments of seafloor effectively, suspension principle of deep-ocean nodule collector is proposed. The supporting and driving mechanisms are separated to avoid collector sinking. The supporting mechanism is a streamlined body with large and smooth supporting area. The grounding pressure is reduced to 0.5- 1 N/cm2 to make sure that the sinkage is limited. The impellers serve as the driving mechanism to supply enough driving power. The position between the supporting mechanism and the driving mechanism can be adjusted according to the operating condition to decrease the walking resistance and to increase driving efficiency. The test results indicate that the collector can walk on the surface of the soft sediments with the limited sinkage. The traction forces were up to 800 kg and the sinkage of the impeller was under the limitation.展开更多
Introduction: Lung nodules are frequently identified on imaging studies and can represent early lung cancers. We instituted the Lung Nodule Evaluation Team(LNET) to optimize management of these nodules by a lung speci...Introduction: Lung nodules are frequently identified on imaging studies and can represent early lung cancers. We instituted the Lung Nodule Evaluation Team(LNET) to optimize management of these nodules by a lung specialist physician. All lung nodules identified by a radiologist prompted a direct consultation to this service. We report our initial experience with this process. Methods: This is a retrospective review of patients with lung nodules at a single institution from 2008 to 2015. Since October 2014, lung nodules >3 mm identified on computed tomography(CT) scanning of the chest generate an automatic consult to LNET from the radiology service. Demographic, nodule and follow up data was entered into a surveillance database and summarized.Results: There were 1,873 patients identified in the database. Of these, 900 patients were undergoing active surveillance. Consults increased from 5.5 to 93 per month after the start of the new consult program. Lung nodules were identified on 64% of chest CT scans. Prior to the direct radiology consult the average size of a nodule was 1.7 cm and 0.7 cm after. The overall time from initial nodule imaging to initiating a management plan by a thoracic specialist physician was 3.7 days. Conclusion: Assessment of lung nodules by a specialist physician is important to ensure appropriate long term management and optimize utilization of diagnostic interventions. A direct radiology consult to a specialized team of chest physicians decreased the time in initiating a management plan, identified smaller nodules and may lead to a more judicious use of health care resources in the management of lung nodules.展开更多
Bedded manganese ore deposits occur in many localities within the accretionary belts of the Shikoku region,SW Japan.The deposits occur mostly in bedded chert or its metamorphosed equivalent.These chert-hosted manganes...Bedded manganese ore deposits occur in many localities within the accretionary belts of the Shikoku region,SW Japan.The deposits occur mostly in bedded chert or its metamorphosed equivalent.These chert-hosted manganese deposits are considered to have been manganese nodule/crust-bearing siliceous sediments on deep-sea floor and have been converted to manganese ores by low-grade metamorphism through subduction-accretion process.The mineral assemblages of the ores reflect the metamorphic grade of the accretionary complexes.On the other hand,iron-manganese deposits and some manganese deposits occurring directly over basalt are considered to have been the hydrothermal precipitates associated with submarine volcanism.展开更多
The root of Alnus sibirica is rich in tissue type. Phloem fibers are obvious and Plasmodesmas are well developed. The number of primary xylem bunches are not stable, there are triarch, tetrarch and pentarch types. Th...The root of Alnus sibirica is rich in tissue type. Phloem fibers are obvious and Plasmodesmas are well developed. The number of primary xylem bunches are not stable, there are triarch, tetrarch and pentarch types. The perforation plate of vessel is scalariform. The rays types are radial in shape and unicellular in structure. In root nodule, cortex is the best developed structure, the stele is small. The root nodule is branched. In root nodule tissues, actinomyces proliferate luxuriently, the actinomyces body is in different shapes.展开更多
基金supported by the grant from China-Japan Friendship Hospital Talent Introduction Research Start-up Fund(to Guang ying ZHU)(No.2016-RC-4)。
文摘Background and objective Low-density computed tomography(LDCT)improved early lung cancer diagnosis but introduces an excess of false-positive pulmonary nodules data.Hence,accurate diagnosis of early-stage lung cancer remains challenging.The purpose of the study was to assess the feasibility of using circulating tumour cells(CTCs)to differentiate malignant from benign pulmonary nodules.Materials and methods 122 patients with suspected malignant pulmonary nodules detected on chest CT in preparation for surgery were prospectively recruited.Peripheral blood samples were collected before surgery,and CTCs were identified upon isolation by size of epithelial tumour cells and morphological analysis.Laser capture microdissection,MALBAC amplification,and whole-exome sequencing were performed on 8 samples.The diagnostic efficacy of CTCs counting,and the genomic variation profile of benign and malignant CTCs samples were analysed.Results Using 2.5 cells/5 m L as the cut-off value,the area under the receiver operating characteristic curve was of 0.651(95%confidence interval:0.538-0.764),with a sensitivity and specificity of 0.526 and 0.800,respectively,and positive and negative predictive values of 91.1%and 30.3%,respectively.Distinct sequence variations differences in DNA damage repair-related and driver genes were observed in benign and malignant samples.TP53 mutations were identified in CTCs of four malignant cases;in particular,g.7578115T>C,g.7578645C>T,and g.7579472G>C were exclusively detected in all four malignant samples.Conclusion CTCs play an ancillary role in the diagnosis of pulmonary nodules.TP53 mutations in CTCs might be used to identify benign and malignant pulmonary nodules.
文摘Viral-mediated gene transfer of thymidine kinase ofherpes simplex virus (HSV-tk) has been used to confercytotoxic sensitivity to ganciclovir (GCV) in a variety oftnmor cells. HSV-tk converts GCV into a phosphorylatedcompound which is toxic for dividing cells by blockingDNA synthesis. Our previous study has shown
基金Projects(51174037,51339008)supported by the National Natural Science Foundation of China
文摘In order to investigate the characteristics of particle-induced pressure loss in the solid–liquid lifting pipe,a series of experiments were conducted in 200 mm diameter lifting pipe.Simulation manganese nodules with five different mean diameters of10 mm,20 mm,30 mm,40 mm and 50 mm were used,both in isolation and a combination in equal fraction by mass.The flow velocities in the lifting pipe ranged from 0.12 m/s to 1.61 m/s,and the mass of particles employed was 10 kg for each particle diameter.Three regimes,wavy bed,partly fluidization,and fully fluidization,were observed over the flow velocity.The solid–liquid pressure drop data were measured by differential pressure transmitter,and pressure drop caused by the solid particles was calculated and analyzed.The results show that the evolutions of the pressure loss due to solid particles are relevant to the solid–liquid flow regimes,and they are distinctly influenced by fluid velocity and particle size.
文摘Marine geology is one of the important mainstays in marine sciences.Since themiddle of this century,due to inumerable systematic investigations,especially the appli-cation of advanced science and technology of various kinds,there has emerged a series ofimportant discoveries in the study of marine geology.These discoveries,while makingcertain traditional basic concepts and theories of geology questionable,led to the birth ofa number of new theories.For example,the discovery of changes in the paleooceanic en-
基金Project(2012AA091201) supported by the National High Technology Research and Development Program of China
文摘To make sure that the nodule collector can walk on the soft sediments of seafloor effectively, suspension principle of deep-ocean nodule collector is proposed. The supporting and driving mechanisms are separated to avoid collector sinking. The supporting mechanism is a streamlined body with large and smooth supporting area. The grounding pressure is reduced to 0.5- 1 N/cm2 to make sure that the sinkage is limited. The impellers serve as the driving mechanism to supply enough driving power. The position between the supporting mechanism and the driving mechanism can be adjusted according to the operating condition to decrease the walking resistance and to increase driving efficiency. The test results indicate that the collector can walk on the surface of the soft sediments with the limited sinkage. The traction forces were up to 800 kg and the sinkage of the impeller was under the limitation.
文摘Introduction: Lung nodules are frequently identified on imaging studies and can represent early lung cancers. We instituted the Lung Nodule Evaluation Team(LNET) to optimize management of these nodules by a lung specialist physician. All lung nodules identified by a radiologist prompted a direct consultation to this service. We report our initial experience with this process. Methods: This is a retrospective review of patients with lung nodules at a single institution from 2008 to 2015. Since October 2014, lung nodules >3 mm identified on computed tomography(CT) scanning of the chest generate an automatic consult to LNET from the radiology service. Demographic, nodule and follow up data was entered into a surveillance database and summarized.Results: There were 1,873 patients identified in the database. Of these, 900 patients were undergoing active surveillance. Consults increased from 5.5 to 93 per month after the start of the new consult program. Lung nodules were identified on 64% of chest CT scans. Prior to the direct radiology consult the average size of a nodule was 1.7 cm and 0.7 cm after. The overall time from initial nodule imaging to initiating a management plan by a thoracic specialist physician was 3.7 days. Conclusion: Assessment of lung nodules by a specialist physician is important to ensure appropriate long term management and optimize utilization of diagnostic interventions. A direct radiology consult to a specialized team of chest physicians decreased the time in initiating a management plan, identified smaller nodules and may lead to a more judicious use of health care resources in the management of lung nodules.
文摘Bedded manganese ore deposits occur in many localities within the accretionary belts of the Shikoku region,SW Japan.The deposits occur mostly in bedded chert or its metamorphosed equivalent.These chert-hosted manganese deposits are considered to have been manganese nodule/crust-bearing siliceous sediments on deep-sea floor and have been converted to manganese ores by low-grade metamorphism through subduction-accretion process.The mineral assemblages of the ores reflect the metamorphic grade of the accretionary complexes.On the other hand,iron-manganese deposits and some manganese deposits occurring directly over basalt are considered to have been the hydrothermal precipitates associated with submarine volcanism.
文摘The root of Alnus sibirica is rich in tissue type. Phloem fibers are obvious and Plasmodesmas are well developed. The number of primary xylem bunches are not stable, there are triarch, tetrarch and pentarch types. The perforation plate of vessel is scalariform. The rays types are radial in shape and unicellular in structure. In root nodule, cortex is the best developed structure, the stele is small. The root nodule is branched. In root nodule tissues, actinomyces proliferate luxuriently, the actinomyces body is in different shapes.
