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基于Needs甲基化法枸杞多糖指纹图谱的建立 被引量:1
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作者 王斌强 文畅 +2 位作者 苏娟 曲欢欢 丁侃 《世界科学技术-中医药现代化》 CSCD 北大核心 2024年第5期1162-1171,共10页
目的多糖是枸杞中主要的活性成分,各产地间枸杞多糖的结构和含量均有差异。目前枸杞多糖的质量控制方法大多都通过单糖组成构建指纹图谱,但仅通过单糖指纹图谱对枸杞多糖进行质量控制,并不能系统地对不同产地的枸杞多糖进行质量控制。因... 目的多糖是枸杞中主要的活性成分,各产地间枸杞多糖的结构和含量均有差异。目前枸杞多糖的质量控制方法大多都通过单糖组成构建指纹图谱,但仅通过单糖指纹图谱对枸杞多糖进行质量控制,并不能系统地对不同产地的枸杞多糖进行质量控制。因此,我们在单糖指纹图谱的基础上,利用Needs甲基化法确定枸杞多糖的糖残基连接方式,建立Needs甲基化法枸杞多糖指纹图谱,实现对不同产地枸杞的质量控制。方法通过水提醇沉法获得枸杞多糖,并采用Needs甲基化、完全酸水解、硼氢化钠还原及乙酰化等方法结合GC-MS测定枸杞多糖的糖残基连接方式。结果将18批枸杞多糖的糖残基连接方式色谱图导入《中药色谱指纹图谱相似度评价系统》(2004版)构建出指纹图谱,并结合多种化学计量法评价不同产地间枸杞多糖的差异。相似度计算结果发现,除西藏自治区三批枸杞多糖相似度0.551-0.569,其余15批样品相似度均0.929以上。糖残基连接方式色谱图共标定了16个共有峰,指认了其中10个共有峰,分别为TArap、T-Araf、T-Xylp、1,2-Arap、1,3-Rhap、1,5-Araf、T-Glcp、T-Galp、1,4-Glcp、1,6-Galp。HCA、PCA和PLS-DA分析结果将18批次不同产地的枸杞多糖分为3类,并筛选出了3个标志性成分,分别为T-Araf、1,5-Araf和T-Glcp,以此来判断不同产地间枸杞多糖的差异性。结论首次建立了18批枸杞多糖的Needs甲基化法指纹图谱,为枸杞多糖质量控制提供实验数据参考,进一步证明了多糖在中药质量控制中的作用。 展开更多
关键词 枸杞多糖 needs甲基化法 糖残基连接方式 指纹图谱 质量控制
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On The Role Of Needs Analysis In ESP Course And Its Implications For Specialized EnglishReading Cour
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作者 刘浩 庞云青 《陕西师范大学学报(哲学社会科学版)》 CSSCI 北大核心 1999年第S1期149-152,共4页
CLCNumber:H319.3Documentcode:AArtrcleID:1000┐5293(1999)Sup.┐0150┐04SpecializedEnglishreadingistheseniorstage... CLCNumber:H319.3Documentcode:AArtrcleID:1000┐5293(1999)Sup.┐0150┐04SpecializedEnglishreadingistheseniorstageincolegeEnglishte... 展开更多
关键词 needs ANALYSIS ESP specialized ENGLISH READING SYLLABUS
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Needs Analysis in ESP Course 被引量:1
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作者 黄奕 《陕西师范大学学报(哲学社会科学版)》 CSSCI 北大核心 2006年第S1期346-349,共4页
From the early 1960’s,English for Specific Purposes(ESP) has grown to become one of the most prominent areas of EFL teaching.This article tends to find a better way to teach ESP with the help of needs analysis.
关键词 ESP ENGLISH TEACHING needs ANALYSIS
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Customization Product Design Based on User's Emotion and Needs 被引量:3
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作者 金纯 张春林 刘文枝 《Defence Technology(防务技术)》 SCIE EI CAS 2005年第2期245-249,共5页
The paper presents a design method that ensures the ingenuity of the product form as well as the whole and exact expression of user’s needs. The key idea is to establish an automatic design system which can transform... The paper presents a design method that ensures the ingenuity of the product form as well as the whole and exact expression of user’s needs. The key idea is to establish an automatic design system which can transform the user’s language needs into the product features in real-time. A rifle was taken as a research instance and soldiers were chosen as evaluation customers. The theory of fuzzy set and semantic difference are adopted to evaluate the relationship between user’s needs and product features as well as their alternatives. FAHP (fuzzy analytic hierarchy process) is utilized to judge the user’s satisfactory forms. This method can also be applied to other product form designs. 展开更多
关键词 user’s needs product form CUSTOMIZATION fuzzy number
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Research Progress of Triploid Rainbow Trout Culture Technology
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作者 Sun Hongqing Ma Lin +5 位作者 You Hongzheng Luo Xin Liu Houfu Meng Muhan Sun Xueliang Bi Xiangdong 《Journal of Northeast Agricultural University(English Edition)》 2025年第1期76-85,共10页
In recent years,the quality of people's lives has gradually improved,and people pay more attention to the rationality of food nutrition.Green and healthy aquatic products,as representative of high-protein and low-... In recent years,the quality of people's lives has gradually improved,and people pay more attention to the rationality of food nutrition.Green and healthy aquatic products,as representative of high-protein and low-fat foods,are increasingly sought after by people.Triploid rainbow trout(Oncorhynchus mykiss)is rich in multiple unsaturated fatty acids and trace elements,which have rich nutrition and delicate flesh,its market demand is increasing.Triploid rainbow trout aquaculture has gradually become a characteristic economic industry in China.The triploid rainbow trout is favored by farmers because of its fast growth rate,delicious meat and large population.However,the development of triploid rainbow trout aquaculture is also restricted by many problems,such as unreasonable breeding management,high feed cost and low processing efficiency.Based on the research reports of domestic and foreign experts and scholars,this paper summarized the research progress of triploid rainbow trout,analyzed the influence of different farming models,nutritional needs,disease prevention on the triploid rainbow trout industry,and put forward suggestions for the development of triploid rainbow trout industry in order to provide scientific reference for the further development of triploid rainbow trout farming technology in China. 展开更多
关键词 triploid rainbow trout breeding mode nutritional needs disease prevention
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Analysis and Research on Information Design of the Smart Community Pension Service 被引量:2
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作者 Chen Hao 《学术界》 CSSCI 北大核心 2018年第8期268-280,共13页
In the research on the smart community pension service,it is found that the problems encountered in the promotion of the smart pension can not be solved in the short term because of the lack of information and the acc... In the research on the smart community pension service,it is found that the problems encountered in the promotion of the smart pension can not be solved in the short term because of the lack of information and the acceptance of the intelligent products by the elderly. This paper puts forward the research ideas based on the cognitive characteristics and demand prediction of the specific population. Focusing on the research on the pension needs of people born in the 1960 s or 1970 s,it provides support for the solution of smart pension services in the peak period of aging population in the future. 展开更多
关键词 SMART COMMUNITY PENSION needs COGNITION of the ELDERLY information design DEMAND forecasting
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Primary stage of solar energy use in architecture-Shadow control 被引量:1
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作者 M.Tahbaz 《Journal of Central South University》 SCIE EI CAS 2012年第3期755-763,共9页
In architecture and urban design,solar energy plays an important role to save fossil energy and preserve environment.Passive or active use of this energy needs especial consideration in the preliminary stage of design... In architecture and urban design,solar energy plays an important role to save fossil energy and preserve environment.Passive or active use of this energy needs especial consideration in the preliminary stage of design to the probable shadows.This work will answer two questions:1) How is it possible to distinguish shadow need periods during a year in a specific climate-2) How is it possible to produce shadow in hot periods without being deprived of pleasant sunshine in cold periods-In this regard,it will introduce the usage process and opportunities of "shading mask" of Olgyay as an easy and useful graphical method in six steps.At the end,different ways of using this method in design will be discussed.Several research projects that have been done in past 30 years will support the argument as examples of this method. 展开更多
关键词 shading mask sun-path chart shadow angle protractor thermal comfort climatic needs calendar
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