The contact characteristics of the rough tooth surface during the meshing process are significantly affected by the lubrication state.The coupling effect of tooth surface roughness and lubrication on meshing character...The contact characteristics of the rough tooth surface during the meshing process are significantly affected by the lubrication state.The coupling effect of tooth surface roughness and lubrication on meshing characteristics of planetary gear is studied.An improved three-dimensional(3 D)anisotropic tooth surface roughness fractal model is proposed based on the experimental parameters.Considering asperity contact and elastohydrodynamic lubrication(EHL),the contact load and flexibility deformation of the tooth surface are derived,and the deformation compatibility equation of the 3 D loaded tooth contact analysis(3 D-LTCA)method is improved.The asperity of the tooth surface changes the system from EHL to mixed lubrication and reduces the stiffness of the oil film.Compared with the sun planet gear,the asperity has a greater effect on the meshing characteristics of the ring-planet gear.Compared with the proposed method,the comprehensive stiffness obtained by the traditional calculation method considering the lubrication effect is smaller,especially for the ring-planet gear.Compared with roughness,speed and viscosity,the meshing characteristics of planetary gears are most sensitive to torque.展开更多
从三维Mesh数据中分割建筑物立面以识别对象,是三维场景理解的关键,但现有方法多依赖高成本的精细标注数据。针对该问题,提出了一种半监督学习方法,引入一种基于对比学习和一致性正则化的半监督语义分割(semi-supervised semantic segme...从三维Mesh数据中分割建筑物立面以识别对象,是三维场景理解的关键,但现有方法多依赖高成本的精细标注数据。针对该问题,提出了一种半监督学习方法,引入一种基于对比学习和一致性正则化的半监督语义分割(semi-supervised semantic segmentation based on contrastive learning and consistency regularization,SS_CC)方法,用于分割三维Mesh数据的建筑物立面。在SS_CC方法中,改进后的对比学习模块利用正负样本之间的类可分性,能够更有效地利用类特征信息;提出的基于特征空间的一致性正则化损失函数,从挖掘全局特征的角度增强了对所提取建筑物立面特征的鉴别力。实验结果表明,所提出的SS_CC方法在F1分数、mIoU指标上优于当前一些主流方法,且在建筑物的墙面和窗户上的分割效果相对更好。展开更多
Gear assembly errors can lead to the increase of vibration and noise of the system,which affect the stability of system.The influence can be compensated by tooth modification.Firstly,an improved three-dimensional load...Gear assembly errors can lead to the increase of vibration and noise of the system,which affect the stability of system.The influence can be compensated by tooth modification.Firstly,an improved three-dimensional loaded tooth contact analysis(3D-LTCA)method which can consider tooth modification and coupling assembly errors is proposed,and mesh stiffness calculated by proposed method is verified by MASTA software.Secondly,based on neural network,the surrogate model(SM)that maps the relationship between modification parameters and mesh mechanical parameters is established,and its accuracy is verified.Finally,SM is introduced to establish an optimization model with the target of minimizing mesh stiffness variations and obtaining more even load distribution on mesh surface.The results show that even considering training time,the efficiency of gear pair optimization by surrogate model is still much higher than that by LTCA method.After optimization,the mesh stiffness fluctuation of gear pair with coupling assembly error is reduced by 34.10%,and difference in average contact stresses between left and right regions of the mesh surface is reduced by 62.84%.展开更多
The aim of this paper is to simulate and study the early moments of the reactive ballistics of a large caliber projectile fired from a gun,combining 0D and 2D axisymmetric Computational Fluid Dynamics(CFD)approaches.F...The aim of this paper is to simulate and study the early moments of the reactive ballistics of a large caliber projectile fired from a gun,combining 0D and 2D axisymmetric Computational Fluid Dynamics(CFD)approaches.First,the methodology is introduced with the development of an interior ballistics(IB)lumped parameter code(LPC),integrating an original image processing method for calculating the specific regression of propellant grains that compose the gun propellant.The ONERA CFD code CEDRE,equipped with a Dynamic Mesh Technique(DMT),is then used in conjunction with the developed LPC to build a dedicated methodology to calculate IB.First results obtained on the AGARD gun and 40 mm gun test cases are in a good agreement with the existing literature.CEDRE is also used to calculate inter-mediate ballistics(first milliseconds of free flight of the projectile)with a multispecies and reactive approach either starting from the gun muzzle plane or directly following IB.In the latter case,an inverse problem involving a Latin hypercube sampling method is used to find a gun propellant configuration that allows the projectile to reach a given exit velocity and base pressure when IB ends.The methodology developed in this work makes it possible to study the flame front of the intermediate flash and depressurization that occurs in a base bleed(BB)channel at the gun muzzle.Average pressure variations in the BB channel during depressurization are in good agreement with literature.展开更多
Importance measures in reliability systems are used to identify weak components in contributing to a proper function of the system. Traditional importance measures mainly concerned the changing value of the system rel...Importance measures in reliability systems are used to identify weak components in contributing to a proper function of the system. Traditional importance measures mainly concerned the changing value of the system reliability caused by the change of the reliability of the component, and seldom considered the joint effect of the probability distribution, improvement rate of the object component. This paper studies the rate of the system reliability upgrading with an improvement of the component reliability for the multi-state consecutive k-out-of-n system. To verify the multi-state consecutive k-out-of-n system reliability upgrading by improving one component based on its improvement rate, an increasing potential importance （IPI） and its physical meaning are described at first. Secondly, the relationship between the IPI and Birnbaum importance measures are discussed. And the IPI for some different improvement actions of the component is further discussed. Thirdly, the characteristics of the IPI are analyzed. Finally, an application to an oil pipeline system is given.展开更多
Classical network reliability problems assume both net- works and components have only binary states, fully working or fully failed states. But many actual networks are multi-state, such as communication networks and ...Classical network reliability problems assume both net- works and components have only binary states, fully working or fully failed states. But many actual networks are multi-state, such as communication networks and transportation networks. The nodes and arcs in the networks may be in intermediate states which are not fully working either fully failed. A simulation ap- proach for computing the two-terminal reliability of a multi-state network is described. Two-terminal reliability is defined as the probability that d units of demand can be supplied from the source to sink nodes under the time threshold T. The capacities of arcs may be in a stochastic state following any discrete or continuous distribution. The transmission time of each arc is also not a fixed number but stochastic according to its current capacity and de- mand. To solve this problem, a capacitated stochastic coloured Petri net is proposed for modelling the system behaviour. Places and transitions respectively stand for the nodes and arcs of a net- work. Capacitated transition and self-modified token colour with route information are defined to describe the multi-state network. By the simulation, the two-terminal reliability and node importance can be estimated and the optimal route whose reliability is highest can also be given. Finally, two examples of different kinds of multi- state networks are given.展开更多
To verify the effectiveness of the integrated importance measure （IIM） for multi-state coherent systems of k level, the definition and physical meaning of IIM are demonstrated. Then, the improvement potential and Δ...To verify the effectiveness of the integrated importance measure （IIM） for multi-state coherent systems of k level, the definition and physical meaning of IIM are demonstrated. Then, the improvement potential and Δ-importance measures are generalized to multi-state coherent systems based on the system performance level, and the relationships between IIM and traditional importance measures are discussed. The characteristics of IIM are demonstrated in both series and parallel systems. Also, an application to an oil transportation system is given. The comparison results show that： （i） IIM has some useful properties that are not possessed by traditional importance measures; （ii） IIM is effective in evaluating the component role in multi-state systems when the component reliability and the failure rate are simultaneously considered.展开更多
The optimal transmission lines assignment with maximal reliabilities (OTLAMR) in the multi-source multi-sink multi-state computer network (MMMCN) was investigated. The OTLAMR problem contains two sub-problems: the MMM...The optimal transmission lines assignment with maximal reliabilities (OTLAMR) in the multi-source multi-sink multi-state computer network (MMMCN) was investigated. The OTLAMR problem contains two sub-problems: the MMMCN reliabilities evaluation and multi-objective transmission lines assignment optimization. First, a reliability evaluation with a transmission line assignment (RETLA) algorithm is proposed to calculate the MMMCN reliabilities under the cost constraint for a certain transmission lines configuration. Second, the non-dominated sorting genetic algorithm II (NSGA-II) is adopted to find the non-dominated set of the transmission lines assignments based on the reliabilities obtained from the RETLA algorithm. By combining the RETLA and the NSGA-II algorithms together, the RETLA-NSGA II algorithm is proposed to solve the OTLAMR problem. The experiments result show that the RETLA-NSGA II algorithm can provide efficient solutions in a reasonable time, from which the decision makers can choose the best solution based on their preferences and experiences.展开更多
The thermal elasto-hydrodynamic lubrication characteristics of the internal meshing gears in a planetary gear train under vibrations were examined considering the influence of the modification coefficient and time-var...The thermal elasto-hydrodynamic lubrication characteristics of the internal meshing gears in a planetary gear train under vibrations were examined considering the influence of the modification coefficient and time-varying meshing stiffness.Based on dynamic theory of the gear system,a dynamic model of the planetary gear train was established.The lubrication performances of modified gear systems under vibrations and static loads were analyzed.Compared with other transmission types,the best lubrication effect could be produced by the positive transmission.A thicker lubricating oil film could be formed,and the friction coefficient and oil film flash temperature are the smallest.Increasing modification coefficient improves the lubrication performance continuously but intensifies the engage-in and tooth-change impact.For the planetary and inner gears,the increase in the modification coefficient also leads a decrease in the oil film stiffness.展开更多
Simulation study on the cylindrical gear meshing with the evolution gear meshing stiffness is being done for better understanding the dynamic characteristics of the kinematics.With consideration of damping,bearing cle...Simulation study on the cylindrical gear meshing with the evolution gear meshing stiffness is being done for better understanding the dynamic characteristics of the kinematics.With consideration of damping,bearing clearance and gear backlash nonlinearity,the dynamic model is set up and computed in MATLAB.The analysis about the relationship between the kinematic responses and the meshing stiffness are carried out.And the results showed that as the gear mesh stiffness is changed from small to large,the performance of the system is changed from the harmonic stable periodic motion to with one times,two times,four times,ending chaos of the stability of the bifurcation.The research results would have theoretical guidance value for the fault diagnosis in engineering.展开更多
At the vascular injury sites,the ultra-large (UL) multimeric von willebrand Factor (VWF) is released in response to physiological and pathophysiological stimuli,and mediates platelet adhesion,aggregation,and cross-lin...At the vascular injury sites,the ultra-large (UL) multimeric von willebrand Factor (VWF) is released in response to physiological and pathophysiological stimuli,and mediates platelet adhesion,aggregation,and cross-linking to maintain hemostasis.This UL-VWF is specifically cleaved by ADAMTS13(A Disintegrin And Metalloprotease with a ThromboSpondintype-1 motif,member 13)to prevent microvascular thrombosis.Each VWF monomer consists of five types of repeat domains in the order of D1-D2-D’-D3-A1-A2-A3-D4-C1-C2-C3-C4-C5-C6-CK,in which the A2 domain contains the ADAMTS13cleavage site(Tyr1605-Met1606),exposure of which requires mechanical or chemical stimuli.Under flows,fluid shear stress regulates VWF degradation and size distribution through opening the A2 domain and exposing its cleavage site for ADAMTS13.VWF A2 domain contains a C-terminal vicinal disulfide bond,a calcium binding sites,and a flexibleα4-less-loop.These unique structure features together make A2 more sensitive to mechanical signal than other VWF A subdomains,i.e.A1 and A3 domains.It is believed that A2 is first bound with and then cleaved by ADAMTS13,together with force-induced conformation transformation.To reveal molecular basis of this two-step model of VWF hydrolyzation by ADAMTS13,we here examined stretch-induced unfolding processes of VWF A2 domain in more detail by Steered molecular dynamics(SMD)simulations,with the use of crystal structure of VWF A2(PDB ID 3GXB),and observed that there were multiple quasi-stable conformations of stretched A2 until itsβ4-strand and a3-helix were pulled away the central hydrophobic core and the cleavage sites were fully accessible to solvent.Our MD simulation data showed that,in unfolding,at first,the cleavage site residue Tyr1605 was exposed partially and binding sites for Spacer domain of ADAMTS13 were exposed to a high level whenα6-helix was separated from A2 body;then,withβ6-strand and a5-helix been pulled away,the binding sites for Cysrich domain of ADAMTS13 was exposed completely while the exposure degree of Tyr1605 was not improved;further,separation ofβ5-strand andα4-less-loop made Tyr1605 and Met1606 and the respective binding sites for ADAMTS13 Spacer domain,Cys-rich domain,and Disintegrin-like domain be fully exposed to reach the optimal catalytic state;lastly,withβ4-strand separation,the cleavage sites and binding sites all were overstretched,leading to mismatch of ADAMTS13 and A2 conformation especially in the binding sites.This conformational mismatch may cause reduction of ADAMTS13 hydrolysis efficiency.Furthermore,the data of SMD simulations under constant forces demonstrated that,the stretched A2 conformation had different quasi-stable states,which all had the better mechanical stability within simulation time of 100 ns;and the conformational transformation from one state to another must overcome their respective potential barriers.The hydrolysis efficiency should depend on each state of the stretched A2 conformation,because of the exclusive matched-degree of A2 and ADMATS13.This computer prediction on the mechanical stability and multi-states of stretched A2 provides a novel insight into the mechano-chemical regulation on cleavage of A2 by ADAMTS13.It would be helpful for design of related drug targeting the binding sites on A2 and exosites on ADAMTS13 for the treatment of patients with acquired TTP.展开更多
Alkaline water electrolysis(AWE)is the most mature technology for hydrogen production by water electrolysis.Alkaline water electrolyzer consists of multiple electrolysis cells,and a single cell consists of a diaphragm...Alkaline water electrolysis(AWE)is the most mature technology for hydrogen production by water electrolysis.Alkaline water electrolyzer consists of multiple electrolysis cells,and a single cell consists of a diaphragm,electrodes,bipolar plates and end plates,etc.The existing industrial bipolar plate channel is concave-convex structure,which is manufactured by complicated and high-cost mold punching.This structure still results in uneven electrolyte flow and low current density in the electrolytic cell,further increasing in energy consumption and cost of AWE.Thereby,in this article,the electrochemical and flow model is firstly constructed,based on the existing industrial concave and convex flow channel structure of bipolar plate,to study the current density,electrolyte flow and bubble distribution in the electrolysis cell.The reliability of the model was verified by comparison with experimental data in literature.Among which,the electrochemical current density affects the bubble yield,on the other hand,the generated bubbles cover the electrode surface,affecting the active specific surface area and ohmic resistance,which in turn affects the electrochemical reaction.The result indicates that the flow velocity near the bottom of the concave ball approaches zero,while the flow velocity on the convex ball surface is significantly higher.Additionally,vortices are observed within the flow channel structure,leading to an uneven distribution of electrolyte.Next,modelling is used to optimize the bipolar plate structure of AWE by simulating the electrochemistry and fluid flow performances of four kinds of structures,namely,concave and convex,rhombus,wedge and expanded mesh,in the bipolar plate of alkaline water electrolyzer.The results show that the expanded mesh channel structure has the largest current density of 3330 A/m^(2)and electrolyte flow velocity of 0.507 m/s in the electrolytic cell.Under the same current density,the electrolytic cell with the expanded mesh runner structure has the smallest potential and energy consumption.This work provides a useful guide for the comprehensive understanding and optimization of channel structures,and a theoretical basis for the design of large-scale electrolyzer.展开更多
基金Project(2024A1515240020)supported by the Guangdong Basic and Applied Basic Research Foundation,China。
文摘The contact characteristics of the rough tooth surface during the meshing process are significantly affected by the lubrication state.The coupling effect of tooth surface roughness and lubrication on meshing characteristics of planetary gear is studied.An improved three-dimensional(3 D)anisotropic tooth surface roughness fractal model is proposed based on the experimental parameters.Considering asperity contact and elastohydrodynamic lubrication(EHL),the contact load and flexibility deformation of the tooth surface are derived,and the deformation compatibility equation of the 3 D loaded tooth contact analysis(3 D-LTCA)method is improved.The asperity of the tooth surface changes the system from EHL to mixed lubrication and reduces the stiffness of the oil film.Compared with the sun planet gear,the asperity has a greater effect on the meshing characteristics of the ring-planet gear.Compared with the proposed method,the comprehensive stiffness obtained by the traditional calculation method considering the lubrication effect is smaller,especially for the ring-planet gear.Compared with roughness,speed and viscosity,the meshing characteristics of planetary gears are most sensitive to torque.
文摘从三维Mesh数据中分割建筑物立面以识别对象,是三维场景理解的关键,但现有方法多依赖高成本的精细标注数据。针对该问题,提出了一种半监督学习方法,引入一种基于对比学习和一致性正则化的半监督语义分割(semi-supervised semantic segmentation based on contrastive learning and consistency regularization,SS_CC)方法,用于分割三维Mesh数据的建筑物立面。在SS_CC方法中,改进后的对比学习模块利用正负样本之间的类可分性,能够更有效地利用类特征信息;提出的基于特征空间的一致性正则化损失函数,从挖掘全局特征的角度增强了对所提取建筑物立面特征的鉴别力。实验结果表明,所提出的SS_CC方法在F1分数、mIoU指标上优于当前一些主流方法,且在建筑物的墙面和窗户上的分割效果相对更好。
基金Project(11972112)supported by the National Natural Science Foundation of ChinaProject(N2103024)supported by the Fundamental Research Funds for the Central Universities,ChinaProject(J2019-IV-0018-0086)supported by the National Science and Technology Major Project,China。
文摘Gear assembly errors can lead to the increase of vibration and noise of the system,which affect the stability of system.The influence can be compensated by tooth modification.Firstly,an improved three-dimensional loaded tooth contact analysis(3D-LTCA)method which can consider tooth modification and coupling assembly errors is proposed,and mesh stiffness calculated by proposed method is verified by MASTA software.Secondly,based on neural network,the surrogate model(SM)that maps the relationship between modification parameters and mesh mechanical parameters is established,and its accuracy is verified.Finally,SM is introduced to establish an optimization model with the target of minimizing mesh stiffness variations and obtaining more even load distribution on mesh surface.The results show that even considering training time,the efficiency of gear pair optimization by surrogate model is still much higher than that by LTCA method.After optimization,the mesh stiffness fluctuation of gear pair with coupling assembly error is reduced by 34.10%,and difference in average contact stresses between left and right regions of the mesh surface is reduced by 62.84%.
基金the French Defense Innovation Agency (AID)the French Procurement Agency for Armament (DGA)ONERA's scientific direction for funding and supporting the present work
文摘The aim of this paper is to simulate and study the early moments of the reactive ballistics of a large caliber projectile fired from a gun,combining 0D and 2D axisymmetric Computational Fluid Dynamics(CFD)approaches.First,the methodology is introduced with the development of an interior ballistics(IB)lumped parameter code(LPC),integrating an original image processing method for calculating the specific regression of propellant grains that compose the gun propellant.The ONERA CFD code CEDRE,equipped with a Dynamic Mesh Technique(DMT),is then used in conjunction with the developed LPC to build a dedicated methodology to calculate IB.First results obtained on the AGARD gun and 40 mm gun test cases are in a good agreement with the existing literature.CEDRE is also used to calculate inter-mediate ballistics(first milliseconds of free flight of the projectile)with a multispecies and reactive approach either starting from the gun muzzle plane or directly following IB.In the latter case,an inverse problem involving a Latin hypercube sampling method is used to find a gun propellant configuration that allows the projectile to reach a given exit velocity and base pressure when IB ends.The methodology developed in this work makes it possible to study the flame front of the intermediate flash and depressurization that occurs in a base bleed(BB)channel at the gun muzzle.Average pressure variations in the BB channel during depressurization are in good agreement with literature.
基金supported by the National Natural Science Foundation of China (71271170 71101116)+1 种基金the National High Technology Research and Development Program of China (863 Progrom) (2012AA040914)the Basic Research Foundation of Northwestern Polytechnical University (JC20120228)
文摘Importance measures in reliability systems are used to identify weak components in contributing to a proper function of the system. Traditional importance measures mainly concerned the changing value of the system reliability caused by the change of the reliability of the component, and seldom considered the joint effect of the probability distribution, improvement rate of the object component. This paper studies the rate of the system reliability upgrading with an improvement of the component reliability for the multi-state consecutive k-out-of-n system. To verify the multi-state consecutive k-out-of-n system reliability upgrading by improving one component based on its improvement rate, an increasing potential importance （IPI） and its physical meaning are described at first. Secondly, the relationship between the IPI and Birnbaum importance measures are discussed. And the IPI for some different improvement actions of the component is further discussed. Thirdly, the characteristics of the IPI are analyzed. Finally, an application to an oil pipeline system is given.
基金supported by the National Natural Science Foundation of China (70971132)
文摘Classical network reliability problems assume both net- works and components have only binary states, fully working or fully failed states. But many actual networks are multi-state, such as communication networks and transportation networks. The nodes and arcs in the networks may be in intermediate states which are not fully working either fully failed. A simulation ap- proach for computing the two-terminal reliability of a multi-state network is described. Two-terminal reliability is defined as the probability that d units of demand can be supplied from the source to sink nodes under the time threshold T. The capacities of arcs may be in a stochastic state following any discrete or continuous distribution. The transmission time of each arc is also not a fixed number but stochastic according to its current capacity and de- mand. To solve this problem, a capacitated stochastic coloured Petri net is proposed for modelling the system behaviour. Places and transitions respectively stand for the nodes and arcs of a net- work. Capacitated transition and self-modified token colour with route information are defined to describe the multi-state network. By the simulation, the two-terminal reliability and node importance can be estimated and the optimal route whose reliability is highest can also be given. Finally, two examples of different kinds of multi- state networks are given.
基金supported by the National Natural Science Foundation of China (7110111671271170)+2 种基金the National Basic Research Program of China (973 Progrom) (2010CB328000)the National High Technology Research and Development Program of China (863 Progrom) (2012AA040914)the Basic Research Foundation of Northwestern Polytechnical University (JC20120228)
文摘To verify the effectiveness of the integrated importance measure （IIM） for multi-state coherent systems of k level, the definition and physical meaning of IIM are demonstrated. Then, the improvement potential and Δ-importance measures are generalized to multi-state coherent systems based on the system performance level, and the relationships between IIM and traditional importance measures are discussed. The characteristics of IIM are demonstrated in both series and parallel systems. Also, an application to an oil transportation system is given. The comparison results show that： （i） IIM has some useful properties that are not possessed by traditional importance measures; （ii） IIM is effective in evaluating the component role in multi-state systems when the component reliability and the failure rate are simultaneously considered.
基金Projects(61004074,61134001,21076179)supported by the National Natural Science Foundation of ChinaProject(2009BAG12A08)supported by the National Key Technology Support Program of China+1 种基金Project(2010QNA5001)supported by the Fundamental Research Funds for the Central Universities of ChinaProjects(2012AA06A404,2006AA04Z184)supported by the National High Technology Research and Development Program of China
文摘The optimal transmission lines assignment with maximal reliabilities (OTLAMR) in the multi-source multi-sink multi-state computer network (MMMCN) was investigated. The OTLAMR problem contains two sub-problems: the MMMCN reliabilities evaluation and multi-objective transmission lines assignment optimization. First, a reliability evaluation with a transmission line assignment (RETLA) algorithm is proposed to calculate the MMMCN reliabilities under the cost constraint for a certain transmission lines configuration. Second, the non-dominated sorting genetic algorithm II (NSGA-II) is adopted to find the non-dominated set of the transmission lines assignments based on the reliabilities obtained from the RETLA algorithm. By combining the RETLA and the NSGA-II algorithms together, the RETLA-NSGA II algorithm is proposed to solve the OTLAMR problem. The experiments result show that the RETLA-NSGA II algorithm can provide efficient solutions in a reasonable time, from which the decision makers can choose the best solution based on their preferences and experiences.
基金Projects(51575289,51705270)supported by the National Natural Science Foundation of China。
文摘The thermal elasto-hydrodynamic lubrication characteristics of the internal meshing gears in a planetary gear train under vibrations were examined considering the influence of the modification coefficient and time-varying meshing stiffness.Based on dynamic theory of the gear system,a dynamic model of the planetary gear train was established.The lubrication performances of modified gear systems under vibrations and static loads were analyzed.Compared with other transmission types,the best lubrication effect could be produced by the positive transmission.A thicker lubricating oil film could be formed,and the friction coefficient and oil film flash temperature are the smallest.Increasing modification coefficient improves the lubrication performance continuously but intensifies the engage-in and tooth-change impact.For the planetary and inner gears,the increase in the modification coefficient also leads a decrease in the oil film stiffness.
基金supported by the National Natural Science Foundation-supported Program(51275052&51575055)
文摘Simulation study on the cylindrical gear meshing with the evolution gear meshing stiffness is being done for better understanding the dynamic characteristics of the kinematics.With consideration of damping,bearing clearance and gear backlash nonlinearity,the dynamic model is set up and computed in MATLAB.The analysis about the relationship between the kinematic responses and the meshing stiffness are carried out.And the results showed that as the gear mesh stiffness is changed from small to large,the performance of the system is changed from the harmonic stable periodic motion to with one times,two times,four times,ending chaos of the stability of the bifurcation.The research results would have theoretical guidance value for the fault diagnosis in engineering.
基金supported by National Natural Science Foundation of China Grants 11672109 ( to Y. F.) and 11432006 ( to J. W.)
文摘At the vascular injury sites,the ultra-large (UL) multimeric von willebrand Factor (VWF) is released in response to physiological and pathophysiological stimuli,and mediates platelet adhesion,aggregation,and cross-linking to maintain hemostasis.This UL-VWF is specifically cleaved by ADAMTS13(A Disintegrin And Metalloprotease with a ThromboSpondintype-1 motif,member 13)to prevent microvascular thrombosis.Each VWF monomer consists of five types of repeat domains in the order of D1-D2-D’-D3-A1-A2-A3-D4-C1-C2-C3-C4-C5-C6-CK,in which the A2 domain contains the ADAMTS13cleavage site(Tyr1605-Met1606),exposure of which requires mechanical or chemical stimuli.Under flows,fluid shear stress regulates VWF degradation and size distribution through opening the A2 domain and exposing its cleavage site for ADAMTS13.VWF A2 domain contains a C-terminal vicinal disulfide bond,a calcium binding sites,and a flexibleα4-less-loop.These unique structure features together make A2 more sensitive to mechanical signal than other VWF A subdomains,i.e.A1 and A3 domains.It is believed that A2 is first bound with and then cleaved by ADAMTS13,together with force-induced conformation transformation.To reveal molecular basis of this two-step model of VWF hydrolyzation by ADAMTS13,we here examined stretch-induced unfolding processes of VWF A2 domain in more detail by Steered molecular dynamics(SMD)simulations,with the use of crystal structure of VWF A2(PDB ID 3GXB),and observed that there were multiple quasi-stable conformations of stretched A2 until itsβ4-strand and a3-helix were pulled away the central hydrophobic core and the cleavage sites were fully accessible to solvent.Our MD simulation data showed that,in unfolding,at first,the cleavage site residue Tyr1605 was exposed partially and binding sites for Spacer domain of ADAMTS13 were exposed to a high level whenα6-helix was separated from A2 body;then,withβ6-strand and a5-helix been pulled away,the binding sites for Cysrich domain of ADAMTS13 was exposed completely while the exposure degree of Tyr1605 was not improved;further,separation ofβ5-strand andα4-less-loop made Tyr1605 and Met1606 and the respective binding sites for ADAMTS13 Spacer domain,Cys-rich domain,and Disintegrin-like domain be fully exposed to reach the optimal catalytic state;lastly,withβ4-strand separation,the cleavage sites and binding sites all were overstretched,leading to mismatch of ADAMTS13 and A2 conformation especially in the binding sites.This conformational mismatch may cause reduction of ADAMTS13 hydrolysis efficiency.Furthermore,the data of SMD simulations under constant forces demonstrated that,the stretched A2 conformation had different quasi-stable states,which all had the better mechanical stability within simulation time of 100 ns;and the conformational transformation from one state to another must overcome their respective potential barriers.The hydrolysis efficiency should depend on each state of the stretched A2 conformation,because of the exclusive matched-degree of A2 and ADMATS13.This computer prediction on the mechanical stability and multi-states of stretched A2 provides a novel insight into the mechano-chemical regulation on cleavage of A2 by ADAMTS13.It would be helpful for design of related drug targeting the binding sites on A2 and exosites on ADAMTS13 for the treatment of patients with acquired TTP.
基金financially supported by the National Natural Science Foundation of China(No.52074130)the Engineering Research Center of Resource Utilization of Carbon-containing Waste with Carbon Neutrality,Ministry of Education。
文摘Alkaline water electrolysis(AWE)is the most mature technology for hydrogen production by water electrolysis.Alkaline water electrolyzer consists of multiple electrolysis cells,and a single cell consists of a diaphragm,electrodes,bipolar plates and end plates,etc.The existing industrial bipolar plate channel is concave-convex structure,which is manufactured by complicated and high-cost mold punching.This structure still results in uneven electrolyte flow and low current density in the electrolytic cell,further increasing in energy consumption and cost of AWE.Thereby,in this article,the electrochemical and flow model is firstly constructed,based on the existing industrial concave and convex flow channel structure of bipolar plate,to study the current density,electrolyte flow and bubble distribution in the electrolysis cell.The reliability of the model was verified by comparison with experimental data in literature.Among which,the electrochemical current density affects the bubble yield,on the other hand,the generated bubbles cover the electrode surface,affecting the active specific surface area and ohmic resistance,which in turn affects the electrochemical reaction.The result indicates that the flow velocity near the bottom of the concave ball approaches zero,while the flow velocity on the convex ball surface is significantly higher.Additionally,vortices are observed within the flow channel structure,leading to an uneven distribution of electrolyte.Next,modelling is used to optimize the bipolar plate structure of AWE by simulating the electrochemistry and fluid flow performances of four kinds of structures,namely,concave and convex,rhombus,wedge and expanded mesh,in the bipolar plate of alkaline water electrolyzer.The results show that the expanded mesh channel structure has the largest current density of 3330 A/m^(2)and electrolyte flow velocity of 0.507 m/s in the electrolytic cell.Under the same current density,the electrolytic cell with the expanded mesh runner structure has the smallest potential and energy consumption.This work provides a useful guide for the comprehensive understanding and optimization of channel structures,and a theoretical basis for the design of large-scale electrolyzer.
