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BDMFuse:Multi-scale network fusion for infrared and visible images based on base and detail features
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作者 SI Hai-Ping ZHAO Wen-Rui +4 位作者 LI Ting-Ting LI Fei-Tao Fernando Bacao SUN Chang-Xia LI Yan-Ling 《红外与毫米波学报》 北大核心 2025年第2期289-298,共10页
The fusion of infrared and visible images should emphasize the salient targets in the infrared image while preserving the textural details of the visible images.To meet these requirements,an autoencoder-based method f... The fusion of infrared and visible images should emphasize the salient targets in the infrared image while preserving the textural details of the visible images.To meet these requirements,an autoencoder-based method for infrared and visible image fusion is proposed.The encoder designed according to the optimization objective consists of a base encoder and a detail encoder,which is used to extract low-frequency and high-frequency information from the image.This extraction may lead to some information not being captured,so a compensation encoder is proposed to supplement the missing information.Multi-scale decomposition is also employed to extract image features more comprehensively.The decoder combines low-frequency,high-frequency and supplementary information to obtain multi-scale features.Subsequently,the attention strategy and fusion module are introduced to perform multi-scale fusion for image reconstruction.Experimental results on three datasets show that the fused images generated by this network effectively retain salient targets while being more consistent with human visual perception. 展开更多
关键词 infrared image visible image image fusion encoder-decoder multi-scale features
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Effects of silica fume on the multi-scale material properties of composite Portland cement-based cutoff wall backfill
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作者 ZHOU Tan HU Jian-hua +2 位作者 ZHAO Feng-wen GUO Meng-meng XUE Sheng-guo 《Journal of Central South University》 2025年第1期205-219,共15页
Soil cement bentonite(SCB)is a common material for constructing vertical cutoff walls to prevent groundwater migration at contaminated industrial sites.However,site contaminants can degrade the durability of the cutof... Soil cement bentonite(SCB)is a common material for constructing vertical cutoff walls to prevent groundwater migration at contaminated industrial sites.However,site contaminants can degrade the durability of the cutoff wall.To enhance its performance,this study developed a silica fume-SCB(SSCB).The macroscopic and microscopic properties of SSCB were assessed by unconfined compressive strength test,variable head permeability test,X-ray diffraction(XRD),scanning electron microscopy(SEM)and nuclear magnetic resonance(NMR)spectroscopy.The correlation between its multi-scale properties was analyzed based on pore characteristics.The results indicate that increasing the silica fume substitution ratio improved SSCB strength,especially in the middle and late curing stages.Moreover,increasing the substitution ratio decreased SSCB permeability coefficient,with a more pronounced effect in earlier curing stages.Silica fume addition also refined SSCB pore structure and reduced its porosity.The fractal dimension was used to quantify SSCB pore structure complexity.Increasing silica fume content reduced small pore fractal dimension in SSCB.Concurrently,SSCB strength increased and SSCB permeability coefficient decreased.The findings of this research will demonstrate the great potential of SSCB backfill for practical applications. 展开更多
关键词 silica fume SSCB cutoff wall multi-scale material properties engineering properties microscopic mechanism
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Disparity estimation for multi-scale multi-sensor fusion
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作者 SUN Guoliang PEI Shanshan +2 位作者 LONG Qian ZHENG Sifa YANG Rui 《Journal of Systems Engineering and Electronics》 SCIE CSCD 2024年第2期259-274,共16页
The perception module of advanced driver assistance systems plays a vital role.Perception schemes often use a single sensor for data processing and environmental perception or adopt the information processing results ... The perception module of advanced driver assistance systems plays a vital role.Perception schemes often use a single sensor for data processing and environmental perception or adopt the information processing results of various sensors for the fusion of the detection layer.This paper proposes a multi-scale and multi-sensor data fusion strategy in the front end of perception and accomplishes a multi-sensor function disparity map generation scheme.A binocular stereo vision sensor composed of two cameras and a light deterction and ranging(LiDAR)sensor is used to jointly perceive the environment,and a multi-scale fusion scheme is employed to improve the accuracy of the disparity map.This solution not only has the advantages of dense perception of binocular stereo vision sensors but also considers the perception accuracy of LiDAR sensors.Experiments demonstrate that the multi-scale multi-sensor scheme proposed in this paper significantly improves disparity map estimation. 展开更多
关键词 stereo vision light deterction and ranging(LiDAR) multi-sensor fusion multi-scale fusion disparity map
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Underwater Image Enhancement Based on Multi-scale Adversarial Network
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作者 ZENG Jun-yang SI Zhan-jun 《印刷与数字媒体技术研究》 CAS 北大核心 2024年第5期70-77,共8页
In this study,an underwater image enhancement method based on multi-scale adversarial network was proposed to solve the problem of detail blur and color distortion in underwater images.Firstly,the local features of ea... In this study,an underwater image enhancement method based on multi-scale adversarial network was proposed to solve the problem of detail blur and color distortion in underwater images.Firstly,the local features of each layer were enhanced into the global features by the proposed residual dense block,which ensured that the generated images retain more details.Secondly,a multi-scale structure was adopted to extract multi-scale semantic features of the original images.Finally,the features obtained from the dual channels were fused by an adaptive fusion module to further optimize the features.The discriminant network adopted the structure of the Markov discriminator.In addition,by constructing mean square error,structural similarity,and perceived color loss function,the generated image is consistent with the reference image in structure,color,and content.The experimental results showed that the enhanced underwater image deblurring effect of the proposed algorithm was good and the problem of underwater image color bias was effectively improved.In both subjective and objective evaluation indexes,the experimental results of the proposed algorithm are better than those of the comparison algorithm. 展开更多
关键词 Underwater image enhancement Generative adversarial network multi-scale feature extraction Residual dense block
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Ship recognition based on HRRP via multi-scale sparse preserving method
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作者 YANG Xueling ZHANG Gong SONG Hu 《Journal of Systems Engineering and Electronics》 SCIE CSCD 2024年第3期599-608,共10页
In order to extract the richer feature information of ship targets from sea clutter, and address the high dimensional data problem, a method termed as multi-scale fusion kernel sparse preserving projection(MSFKSPP) ba... In order to extract the richer feature information of ship targets from sea clutter, and address the high dimensional data problem, a method termed as multi-scale fusion kernel sparse preserving projection(MSFKSPP) based on the maximum margin criterion(MMC) is proposed for recognizing the class of ship targets utilizing the high-resolution range profile(HRRP). Multi-scale fusion is introduced to capture the local and detailed information in small-scale features, and the global and contour information in large-scale features, offering help to extract the edge information from sea clutter and further improving the target recognition accuracy. The proposed method can maximally preserve the multi-scale fusion sparse of data and maximize the class separability in the reduced dimensionality by reproducing kernel Hilbert space. Experimental results on the measured radar data show that the proposed method can effectively extract the features of ship target from sea clutter, further reduce the feature dimensionality, and improve target recognition performance. 展开更多
关键词 ship target recognition high-resolution range profile(HRRP) multi-scale fusion kernel sparse preserving projection(MSFKSPP) feature extraction dimensionality reduction
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Unconditionally stable Crank-Nicolson algorithm with enhanced absorption for rotationally symmetric multi-scale problems in anisotropic magnetized plasma
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作者 WEN Yi WANG Junxiang XU Hongbing 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2024年第1期65-73,共9页
Large calculation error can be formed by directly employing the conventional Yee’s grid to curve surfaces.In order to alleviate such condition,unconditionally stable CrankNicolson Douglas-Gunn(CNDG)algorithm with is ... Large calculation error can be formed by directly employing the conventional Yee’s grid to curve surfaces.In order to alleviate such condition,unconditionally stable CrankNicolson Douglas-Gunn(CNDG)algorithm with is proposed for rotationally symmetric multi-scale problems in anisotropic magnetized plasma.Within the CNDG algorithm,an alternative scheme for the simulation of anisotropic plasma is proposed in body-of-revolution domains.Convolutional perfectly matched layer(CPML)formulation is proposed to efficiently solve the open region problems.Numerical example is carried out for the illustration of effectiveness including the efficiency,resources,and absorption.Through the results,it can be concluded that the proposed scheme shows considerable performance during the simulation. 展开更多
关键词 anisotropic magnetized plasma body-of-revolution(BOR) Crank-Nicolson Douglas-Gunn(CNDG) finite-difference time-domain(FDTD) perfectly matched layer(PML) rotationally symmetric multi-scale problems
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基于Annotation的Spring AOP系统设计 被引量:7
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作者 刘荣辉 薛冰 《计算机应用与软件》 CSCD 2009年第9期18-20,共3页
简要介绍AOP(Aspect Oriented Programming)技术和Annotation技术,讨论了Spring中的AOP的两种实现。通过同一个案例阐述了在Spring中通过XML配置来实现AOP和使用了Annotation在Spring中实现AOP,比较了两种实现方式的优缺点。对在Spring... 简要介绍AOP(Aspect Oriented Programming)技术和Annotation技术,讨论了Spring中的AOP的两种实现。通过同一个案例阐述了在Spring中通过XML配置来实现AOP和使用了Annotation在Spring中实现AOP,比较了两种实现方式的优缺点。对在Spring使用Annotation实现AOP作了总结,从而体现了Annotation在设计系统时其简洁性和良好的扩展性。 展开更多
关键词 SPRING XML AOP annotation
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An algorithm for segmentation of lung ROI by mean-shift clustering combined with multi-scale HESSIAN matrix dot filtering 被引量:7
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作者 魏颖 李锐 +1 位作者 杨金柱 赵大哲 《Journal of Central South University》 SCIE EI CAS 2012年第12期3500-3509,共10页
A new algorithm for segmentation of suspected lung ROI(regions of interest)by mean-shift clustering and multi-scale HESSIAN matrix dot filtering was proposed.Original image was firstly filtered by multi-scale HESSIAN ... A new algorithm for segmentation of suspected lung ROI(regions of interest)by mean-shift clustering and multi-scale HESSIAN matrix dot filtering was proposed.Original image was firstly filtered by multi-scale HESSIAN matrix dot filters,round suspected nodular lesions in the image were enhanced,and linear shape regions of the trachea and vascular were suppressed.Then,three types of information,such as,shape filtering value of HESSIAN matrix,gray value,and spatial location,were introduced to feature space.The kernel function of mean-shift clustering was divided into product form of three kinds of kernel functions corresponding to the three feature information.Finally,bandwidths were calculated adaptively to determine the bandwidth of each suspected area,and they were used in mean-shift clustering segmentation.Experimental results show that by the introduction of HESSIAN matrix of dot filtering information to mean-shift clustering,nodular regions can be segmented from blood vessels,trachea,or cross regions connected to the nodule,non-nodular areas can be removed from ROIs properly,and ground glass object(GGO)nodular areas can also be segmented.For the experimental data set of 127 different forms of nodules,the average accuracy of the proposed algorithm is more than 90%. 展开更多
关键词 HESSIAN matrix multi-scale dot filtering mean-shift clustering segmentation of suspected areas lung computer-aideddetection/diagnosis
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Fast-armored target detection based on multi-scale representation and guided anchor 被引量:6
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作者 Fan-jie Meng Xin-qing Wang +2 位作者 Fa-ming Shao Dong Wang Xiao-dong Hu 《Defence Technology(防务技术)》 SCIE EI CAS CSCD 2020年第4期922-932,共11页
Focused on the task of fast and accurate armored target detection in ground battlefield,a detection method based on multi-scale representation network(MS-RN) and shape-fixed Guided Anchor(SF-GA)scheme is proposed.Firs... Focused on the task of fast and accurate armored target detection in ground battlefield,a detection method based on multi-scale representation network(MS-RN) and shape-fixed Guided Anchor(SF-GA)scheme is proposed.Firstly,considering the large-scale variation and camouflage of armored target,a new MS-RN integrating contextual information in battlefield environment is designed.The MS-RN extracts deep features from templates with different scales and strengthens the detection ability of small targets.Armored targets of different sizes are detected on different representation features.Secondly,aiming at the accuracy and real-time detection requirements,improved shape-fixed Guided Anchor is used on feature maps of different scales to recommend regions of interests(ROIs).Different from sliding or random anchor,the SF-GA can filter out 80% of the regions while still improving the recall.A special detection dataset for armored target,named Armored Target Dataset(ARTD),is constructed,based on which the comparable experiments with state-of-art detection methods are conducted.Experimental results show that the proposed method achieves outstanding performance in detection accuracy and efficiency,especially when small armored targets are involved. 展开更多
关键词 RED image RPN Fast-armored target detection based on multi-scale representation and guided anchor
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Attention mechanism based multi-scale feature extraction of bearing fault diagnosis 被引量:4
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作者 LEI Xue LU Ningyun +2 位作者 CHEN Chuang HU Tianzhen JIANG Bin 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2023年第5期1359-1367,共9页
Effective bearing fault diagnosis is vital for the safe and reliable operation of rotating machinery.In practical applications,bearings often work at various rotational speeds as well as load conditions.Yet,the bearin... Effective bearing fault diagnosis is vital for the safe and reliable operation of rotating machinery.In practical applications,bearings often work at various rotational speeds as well as load conditions.Yet,the bearing fault diagnosis under multiple conditions is a new subject,which needs to be further explored.Therefore,a multi-scale deep belief network(DBN)method integrated with attention mechanism is proposed for the purpose of extracting the multi-scale core features from vibration signals,containing four primary steps:preprocessing of multi-scale data,feature extraction,feature fusion,and fault classification.The key novelties include multi-scale feature extraction using multi-scale DBN algorithm,and feature fusion using attention mecha-nism.The benchmark dataset from University of Ottawa is applied to validate the effectiveness as well as advantages of this method.Furthermore,the aforementioned method is compared with four classical fault diagnosis methods reported in the literature,and the comparison results show that our pro-posed method has higher diagnostic accuracy and better robustness. 展开更多
关键词 bearing fault diagnosis multiple conditions atten-tion mechanism multi-scale data deep belief network(DBN)
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Radar emitter signal recognition based on multi-scale wavelet entropy and feature weighting 被引量:16
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作者 李一兵 葛娟 +1 位作者 林云 叶方 《Journal of Central South University》 SCIE EI CAS 2014年第11期4254-4260,共7页
In modern electromagnetic environment, radar emitter signal recognition is an important research topic. On the basis of multi-resolution wavelet analysis, an adaptive radar emitter signal recognition method based on m... In modern electromagnetic environment, radar emitter signal recognition is an important research topic. On the basis of multi-resolution wavelet analysis, an adaptive radar emitter signal recognition method based on multi-scale wavelet entropy feature extraction and feature weighting was proposed. With the only priori knowledge of signal to noise ratio(SNR), the method of extracting multi-scale wavelet entropy features of wavelet coefficients from different received signals were combined with calculating uneven weight factor and stability weight factor of the extracted multi-dimensional characteristics. Radar emitter signals of different modulation types and different parameters modulated were recognized through feature weighting and feature fusion. Theoretical analysis and simulation results show that the presented algorithm has a high recognition rate. Additionally, when the SNR is greater than-4 d B, the correct recognition rate is higher than 93%. Hence, the proposed algorithm has great application value. 展开更多
关键词 emitter recognition multi-scale wavelet entropy feature weighting uneven weight factor stability weight factor
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Vibration analysis of fluid-conveying multi-scale hybrid nanocomposite shells with respect to agglomeration of nanofillers 被引量:2
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作者 Farzad Ebrahimi Ali Dabbagh 《Defence Technology(防务技术)》 SCIE EI CAS CSCD 2021年第1期212-225,共14页
The vibration problem of a fluid conveying cylindrical shell consisted of newly developed multi-scale hybrid nanocomposites is solved in the present manuscript within the framework of an analytical solution.The consis... The vibration problem of a fluid conveying cylindrical shell consisted of newly developed multi-scale hybrid nanocomposites is solved in the present manuscript within the framework of an analytical solution.The consistent material is considered to be made from an initial matrix strengthened via both macro-and nano-scale reinforcements.The influence of nanofillers’agglomeration,generated due to the high surface to volume ratio in nanostructures,is included by implementing Eshelby-Mori-Tanaka homogenization scheme.Afterwards,the equivalent material properties of the carbon nanotube reinforced(CNTR)nanocomposite are coupled with those of CFs within the framework of a modified rule of mixture.On the other hand,the influences of viscous flow are covered by extending the Navier-Stokes equation for cylinders.A cylindrical coordinate system is chosen and mixed with the infinitesimal strains of first-order shear deformation theory of shells to obtain the motion equations on the basis of the dynamic form of principle of virtual work.Next,the achieved governing equations will be solved by Galerkin’s method to reach the natural frequency of the structure for both simply supported and clamped boundary conditions.Presenting a set of illustrations,effects of each parameter on the dimensionless frequency of nanocomposite shells will be shown graphically. 展开更多
关键词 Vibration Agglomeration effect multi-scale hybrid nanocomposites Galerkin’s solution Viscous fluid flow
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一种基于.NET Services的标注系统框架“MyAnnotation.NET”
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作者 谢波 申瑞民 王加俊 《计算机工程》 CAS CSCD 北大核心 2003年第16期179-181,共3页
“MyAnnotation.NET”是一种自动收集、管理、检索和共享个人信息的标准的开放的与操作系统和具体应用无关的通用标注框架 (Annotation Framework),它以.NET Services的形式提供标准化、模块化的网络服务(Web Service)。该文介绍了... “MyAnnotation.NET”是一种自动收集、管理、检索和共享个人信息的标准的开放的与操作系统和具体应用无关的通用标注框架 (Annotation Framework),它以.NET Services的形式提供标准化、模块化的网络服务(Web Service)。该文介绍了它的体系架构、核心算法以及一个e-Learning领域的实施案例。 展开更多
关键词 标注榧架 .NET SERVICES SDT VITERBI TFIDF
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Multi-scale and multi-fractal analysis of pressure fluctuation in slurry bubble column bed reactor 被引量:1
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作者 王兴军 胡立舜 +3 位作者 沈军杰 余志楠 王辅臣 于遵宏 《Journal of Central South University of Technology》 EI 2007年第5期696-700,共5页
The Daubechies second order wavelet was applied to decompose pressure fluctuation signals with the gas flux varying from 0.18 to 0.90 m3/h and the solid mass fraction from 0 to 20% and scales 1?9 detail signals and th... The Daubechies second order wavelet was applied to decompose pressure fluctuation signals with the gas flux varying from 0.18 to 0.90 m3/h and the solid mass fraction from 0 to 20% and scales 1?9 detail signals and the 9th scale approximation signals. The pressure signals were studied by multi-scale and R/S analysis method. Hurst analysis method was applied to analyze multi-fractal characteristics of different scale signals. The results show that the characteristics of mono-fractal under scale 1 and scale 2, and bi-fractal under scale 3?9 are effective in deducing the hydrodynamics in slurry bubbling flow system. The measured pressure signals are decomposed to micro-scale signals, meso-scale signals and macro-scale signals. Micro-scale and macro-scale signals are of mono-fractal characteristics, and meso-scale signals are of bi-fractal characteristics. By analyzing energy distribution of different scale signals,it is shown that pressure fluctuations mainly reflects meso-scale interaction between the particles and the bubble. 展开更多
关键词 pressure fluctuation R/S analysis multi-scale MULTI-FRACTAL bubble column bed reactor
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A Hierarchical Image Annotation Method Based on SVM and Semi-supervised EM 被引量:8
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作者 GAO Yan-Yu YIN Yi-Xin UOZUMI Takashi 《自动化学报》 EI CSCD 北大核心 2010年第7期960-967,共8页
关键词 数字图像 自动化系统 准确性 意义 图像标注
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Multi-scale regionalization based mining of spatio-temporal teleconnection patterns between anomalous sea and land climate events
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作者 XU Feng SHI Yan +3 位作者 DENG Min GONG Jian-ya LIU Qi-liang JIN Rui 《Journal of Central South University》 SCIE EI CAS CSCD 2017年第10期2438-2448,共11页
Climate sequences can be applied to defining sensitive climate zones, and then the mining of spatio-temporal teleconnection patterns is useful for learning from the past and preparing for the future. However, scale-de... Climate sequences can be applied to defining sensitive climate zones, and then the mining of spatio-temporal teleconnection patterns is useful for learning from the past and preparing for the future. However, scale-dependency in this kind of pattern is still not well handled by existing work. Therefore, in this study, the multi-scale regionalization is embedded into the spatio-temporal teleconnection pattern mining between anomalous sea and land climatic events. A modified scale-space clustering algorithm is first developed to group climate sequences into multi-scale climate zones. Then, scale variance analysis method is employed to identify climate zones at characteristic scales, indicating the main characteristics of geographical phenomena. Finally, by using the climate zones identified at characteristic scales, a time association rule mining algorithm based on sliding time windows is employed to discover spatio-temporal teleconnection patterns. Experiments on sea surface temperature, sea level pressure, land precipitation and land temperature datasets show that many patterns obtained by the multi-scale approach are coincident with prior knowledge, indicating that this method is effective and reasonable. In addition, some unknown teleconnection patterns discovered from the multi-scale approach can be further used to guide the prediction of land climate. 展开更多
关键词 CLIMATE sequences ANOMALOUS climatic EVENTS SPATIO-TEMPORAL teleconnection patterns multi-scale REGIONALIZATION
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Global Annotation of Small RNA and MicroRNA Mature Sequences from Developing Ovules of Gossypium hirsutum L.
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作者 ABDURAKHMONOV Ibrokhim Y DEVOR Eric J BURIEV Zabardast T MAKAMOV Abdusalom SHERMATOV Shukhrat E ABDUKARIMOV Abdusattor 《棉花学报》 CSCD 北大核心 2008年第S1期11-,共1页
The involvement of small RNAs in cotton fiber development is under explored.The objective of this work was to directly clone,annotate,and analyze small RNAs of developing ovules to reveal
关键词 Global annotation of Small RNA and MicroRNA Mature Sequences from Developing Ovules of Gossypium hirsutum L RNA
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Cloning,Characterization,and Gene Annotation of Cellulose Synthase Genes from Arabidopsis thaliana
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作者 BALASUBRAMANI G AMUDHA J KATEGERI I S KHADI B M 《棉花学报》 CSCD 北大核心 2008年第S1期50-,共1页
The mechanistic basis of cellulose biosynthesis in plants has gained ground during last decade or so.The isolation of plant cDNA clones encoding cotton homologs of the bacterial cellulose
关键词 Cloning Characterization and Gene annotation of Cellulose Synthase Genes from Arabidopsis thaliana
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1株猪源动物联合乳杆菌S7全基因组测序及生物信息学分析
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作者 刘辉 季海峰 +2 位作者 王四新 陈美霞 张董燕 《中国畜牧兽医》 北大核心 2025年第1期25-38,共14页
[目的]旨在对1株具有较强耐酸耐胆盐特性的猪源动物联合乳杆菌S7进行全基因组测序及生物信息学分析。[方法]结合三代PacBio RSⅡ和二代Illumina HiSeq 2000测序技术对动物联合乳杆菌S7进行了全基因组测序,在此基础上使用GO、EggNOG、KEG... [目的]旨在对1株具有较强耐酸耐胆盐特性的猪源动物联合乳杆菌S7进行全基因组测序及生物信息学分析。[方法]结合三代PacBio RSⅡ和二代Illumina HiSeq 2000测序技术对动物联合乳杆菌S7进行了全基因组测序,在此基础上使用GO、EggNOG、KEGG、CAZy、VFDB和CARD等数据库注释功能基因,利用TYGS(Type Strain Genome Server)构建系统发育树,并与2株同源模式菌株进行了共线性分析。[结果]动物联合乳杆菌S7的基因组大小为2.03 Mb, GC含量为44.14%,共预测到1 993个编码基因,包含65个tRNA、19个rRNA、35个sRNA、29个持家基因、11个基因组岛、6个前噬菌体、4个CRISPR-Cas、8个插入序列和10个转座子;分别有1 521、1 567、1 185和60个基因在GO、EggNOG、KEGG和CAZy数据库中得到注释;在VFDB和CARD数据库中注释到181个毒力基因和110个耐药基因;另外有14个基因参与耐酸,2个基因参与耐胆盐,22个基因参与黏附和聚集;基因组中还包括与温度应激、氧化应激和细菌素合成等多种与益生功能相关的基因。系统发育树和共线性分析发现,该菌株与模式菌株Ligilactobacillus animalis P38的进化关系最近。[结论]动物联合乳杆菌S7基因组中具有与耐酸、耐胆盐、黏附和聚集、温度应激、氧化应激和细菌素等相关的功能基因,可为菌株在饲料添加剂中的科学应用提供理论依据。 展开更多
关键词 动物联合乳杆菌 全基因组测序 基因注释
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茶银尺蠖触角转录组及相关嗅觉基因的分析
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作者 张方梅 尤伟晨 +5 位作者 郭世保 陈俊华 史洪中 耿书宝 周洲 乔利 《环境昆虫学报》 北大核心 2025年第2期504-514,共11页
本研究利用IIIumina Hiseq 4000高通量测序平台对茶银尺蠖Scopula subpunctaria(Herrich-Schaeffer)雌雄成虫触角进行转录组测序,共获得93335条unigenes,N50长度为1151 bp。通过基因功能注释,有23581条unigenes注释到六大公共数据库中,... 本研究利用IIIumina Hiseq 4000高通量测序平台对茶银尺蠖Scopula subpunctaria(Herrich-Schaeffer)雌雄成虫触角进行转录组测序,共获得93335条unigenes,N50长度为1151 bp。通过基因功能注释,有23581条unigenes注释到六大公共数据库中,其中注释到NR数据库的unigenes最多,占39.96%,且与家蚕Bombyx mori相似基因数目最多,占20.32%;10718条unigenes注释到GO数据库的生物过程、细胞组分和分子功能3个大类46个亚类;5464条unigenes注释到KEGG数据库的38个小类代谢通路,其中注释在信号传导通路的unigenes最多,占10.64%。根据注释信息,共筛选得到60个候选嗅觉基因,包含17个气味结合蛋白基因(Odorant binding protein genes,OBPs)、13个化学感受蛋白基因(Chemosensory protein genes,CSPs)、14个嗅觉受体基因(Olfactory receptor genes,ORs)、13个离子型受体基因(Ionotropic receptor genes,IRs)和3个感觉神经膜蛋白基因(Sensory neuron membrane protein genes,SNMPs)。利用FPKM值对SsubOBPs和SsubCSPs基因进行表达量分析发现,SsubOBP2和SsubOBP3在茶银尺蠖雌雄成虫触角中均高丰度表达(FPKM>2000),SsubCSP4、SsubCSP7和SsubCSP9在茶银尺蠖雌雄成虫触角中均高丰度表达(FPKM>900)。本研究首次获得了茶银尺蠖触角转录组数据,并鉴定出候选嗅觉相关基因,结果为进一步研究茶银尺蠖的嗅觉感受机制提供分子基础。 展开更多
关键词 茶银尺蠖 触角转录组 嗅觉相关蛋白 基因注释
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