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运用Real-time quantification PCR方法建立副溶血性弧菌在即食虾中的生长预测模型 被引量:5
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作者 彭织云 王敬敬 +2 位作者 唐晓阳 潘迎捷 赵勇 《食品工业科技》 CAS CSCD 北大核心 2013年第8期108-110,共3页
运用Real-time quantification PCR(real-time qPCR)方法建立副溶血性弧菌在即食虾中生长预测模型。首先构建质粒标准品,梯度稀释后建立标准曲线,然后用Real-time qPCR方法检测虾中副溶血性弧菌的数量,最后建立37℃下即食虾中副溶血性... 运用Real-time quantification PCR(real-time qPCR)方法建立副溶血性弧菌在即食虾中生长预测模型。首先构建质粒标准品,梯度稀释后建立标准曲线,然后用Real-time qPCR方法检测虾中副溶血性弧菌的数量,最后建立37℃下即食虾中副溶血性弧菌生长预测模型,并与传统涂布计数方法进行比较。结果表明,Real-time qPCR方法和传统计数方法均可建立Gmopertz模型、Logistic模型和Richards模型,模型拟合的相关系数R2均在0.9以上。基于Real-timeqPCR方法省时省力、特异性好等优点,用Real-time qPCR方法建立微生物预测模型是未来预测微生物学领域的一种发展趋势。 展开更多
关键词 REAL-TIME quantification PCR 副溶血性弧菌 生长预测模型
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规划设计学中的调查分析法14—数量化Ⅰ类(Quantification Theory Type Ⅰ) 被引量:1
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作者 章俊华 《中国园林》 2003年第9期47-50,共4页
回归及重回归分析和预测,被广泛地应用于各个行业中。但是,有些相当于回归分析中的说明变数,不是数量数据,而是非数量数据,例如:影响入园人数的因素有星期(平日、休日)、天气状况(晴天、阴天、雨雪天等)、季节(春、夏、秋、冬)等。这些... 回归及重回归分析和预测,被广泛地应用于各个行业中。但是,有些相当于回归分析中的说明变数,不是数量数据,而是非数量数据,例如:影响入园人数的因素有星期(平日、休日)、天气状况(晴天、阴天、雨雪天等)、季节(春、夏、秋、冬)等。这些因素因为不是“量”的数据,所以不能用回归或重回归等方法进行分析和预测,但这种情况在规划设计的调查中经常出现,所以数量化Ⅰ类,可以帮助人们进行与回归分析和预测相同的分析,这是十分有效的一种分析方法。 展开更多
关键词 风景园林 规划设计学 调查 数量化I类
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Computational intelligence approach for uncertainty quantification using evidence theory 被引量:4
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作者 Bin Suo Yongsheng Cheng +1 位作者 Chao Zeng Jun Li 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2013年第2期250-260,共11页
As an alternative or complementary approach to the classical probability theory,the ability of the evidence theory in uncertainty quantification(UQ) analyses is subject of intense research in recent years.Two state-... As an alternative or complementary approach to the classical probability theory,the ability of the evidence theory in uncertainty quantification(UQ) analyses is subject of intense research in recent years.Two state-of-the-art numerical methods,the vertex method and the sampling method,are commonly used to calculate the resulting uncertainty based on the evidence theory.The vertex method is very effective for the monotonous system,but not for the non-monotonous one due to its high computational errors.The sampling method is applicable for both systems.But it always requires a high computational cost in UQ analyses,which makes it inefficient in most complex engineering systems.In this work,a computational intelligence approach is developed to reduce the computational cost and improve the practical utility of the evidence theory in UQ analyses.The method is demonstrated on two challenging problems proposed by Sandia National Laboratory.Simulation results show that the computational efficiency of the proposed method outperforms both the vertex method and the sampling method without decreasing the degree of accuracy.Especially,when the numbers of uncertain parameters and focal elements are large,and the system model is non-monotonic,the computational cost is five times less than that of the sampling method. 展开更多
关键词 uncertainty quantification(UQ) evidence theory hybrid algorithm interval algorithm genetic algorithm(GA).
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Multi-component opportunistic maintenance optimization for wind turbines with consideration of seasonal factor 被引量:4
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作者 SU Chun HU Zhao-yong LIU Yang 《Journal of Central South University》 SCIE EI CAS CSCD 2020年第2期490-499,共10页
Aiming at wind turbines,the opportunistic maintenance optimization is carried out for multi-component system,where minimal repair,imperfect repair,replacement as well as their effects on component’s effective age are... Aiming at wind turbines,the opportunistic maintenance optimization is carried out for multi-component system,where minimal repair,imperfect repair,replacement as well as their effects on component’s effective age are considered.At each inspection point,appropriate maintenance mode is selected according to the component’s effective age and its maintenance threshold.To utilize the maintenance opportunities for the components among the wind turbines,opportunistic maintenance approach is adopted.Meanwhile,the influence of seasonal factor on the component’s failure rate and improvement factor’s decrease with the increase of repair’s times are also taken into account.The maintenance threshold is set as the decision variable,and an opportunistic maintenance optimization model is proposed to minimize wind turbine’s life-cycle maintenance cost.Moreover,genetic algorithm is adopted to solve the model,and the effectiveness is verified with a case study.The results show that based on the component’s inherent reliability and maintainability,the proposed model can provide optimal maintenance plans accordingly.Furthermore,the higher the component’s reliability and maintainability are,the less the times of repair and replacement will be. 展开更多
关键词 wind turbine RELIABILITY seasonal factor multi-component maintenance opportunistic maintenance
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Layout problem of multi-component systems arising for improving maintainability 被引量:5
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作者 罗旭 杨拥民 +2 位作者 葛哲学 温熙森 官凤娇 《Journal of Central South University》 SCIE EI CAS 2014年第5期1833-1841,共9页
To improve the mainlainability design efficiency and quality, a layout optimization method for maintainability of multi-component systems was proposed. The impact of the component layout design on system maintainabili... To improve the mainlainability design efficiency and quality, a layout optimization method for maintainability of multi-component systems was proposed. The impact of the component layout design on system maintainability was analyzed, and the layout problem for maintainability was presented. It was formulated as an optimization problem, where maintainability, layout space and distance requirement were formulated as objective functions. A multi-objective particle swarm optimization algorithm, in which the constrained-domination relationship and the update strategy of the global best were simply modified, was then used to obtain Pareto optimal solutions for the maintainability layout design problem. Finally, application in oxygen generation system of a spacecraft was studied in detail to illustrate the effectiveness and usefulness of the proposed method. The results show that the concurrent maintainability design can be carried out during the layout design process by solving the layout optimization problem for maintainability. 展开更多
关键词 MAINTAINABILITY layout problem OPTIMIZATION multi-component system multi-objective particle swarm optimization
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Opportunistic maintenance for multi-component systems considering structural dependence and economic dependence 被引量:6
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作者 Junbao Geng Michael Azarian Michael Pecht 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2015年第3期493-501,共9页
Although opportunistic maintenance strategies are widely used for multi-component systems, all opportunistic mainte- nance strategies only consider economic dependence and do not take structural dependence into accoun... Although opportunistic maintenance strategies are widely used for multi-component systems, all opportunistic mainte- nance strategies only consider economic dependence and do not take structural dependence into account. An opportunistic main- tenance strategy is presented for a multi-component system that considers both structural dependence and economic dependence. The cost relation and time relation among components based on structural dependence are developed. The maintenance strategy for each component of a multi-component system involves one of five maintenance actions, namely, no-maintenance, a minimal maintenance action, an imperfect maintenance action, a perfect maintenance action, and a replacement action. The maintenance action is determined by the virtual age of the component, the life expectancy of the component, and the age threshold values. Monte Carlo simulation is designed to obtain the optimal oppor- tunistic maintenance strategy of the system over its lifetime. The simulation result reveals that the minimum maintenance cost with a strategy that considers structural dependence is less than that with a strategy that does not consider structural dependence. The availability with a strategy that considers structural dependence is greater than that with a strategy that does not consider structural dependence under the same conditions. 展开更多
关键词 opportunistic maintenance multi-component system structural dependence Monte Carlo simulation maintenance cost.
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Dynamic probabilistic design technique for multi-component system with multi-failure modes 被引量:4
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作者 ZHANG Chun-yi LU Cheng +2 位作者 FEI Cheng-wei JING Hui-zhe LI Cheng-wei 《Journal of Central South University》 SCIE EI CAS CSCD 2018年第11期2688-2700,共13页
For unacceptable computational efficiency and accuracy on the probabilistic analysis of multi-component system with multi-failure modes,this paper proposed multi-extremum response surface method(MERSM).MERSM model was... For unacceptable computational efficiency and accuracy on the probabilistic analysis of multi-component system with multi-failure modes,this paper proposed multi-extremum response surface method(MERSM).MERSM model was established based on quadratic polynomial function by taking extremum response surface model as the sub-model of multi-response surface method.The dynamic probabilistic analysis of an aeroengine turbine blisk with two components,and their reliability of deformation and stress failures was obtained,based on thermal-structural coupling technique,by considering the nonlinearity of material parameters and the transients of gas flow,gas temperature and rotational speed.The results show that the comprehensive reliability of structure is 0.9904 when the allowable deformations and stresses of blade and disk are 4.78×10–3 m and 1.41×109 Pa,and 1.64×10–3 m and 1.04×109 Pa,respectively.Besides,gas temperature and rotating speed severely influence the comprehensive reliability of system.Through the comparison of methods,it is shown that the MERSM holds higher computational precision and speed in the probabilistic analysis of turbine blisk,and MERSM computational precision satisfies the requirement of engineering design.The efforts of this study address the difficulties on transients and multiple models coupling for the dynamic probabilistic analysis of multi-component system with multi-failure modes. 展开更多
关键词 probabilistic analysis multi-extremum response surface method multi-component multi-failure modes
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High-dimensional uncertainty quantification of projectile motion in the barrel of a truck-mounted howitzer based on probability density evolution method 被引量:1
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作者 Mingming Wang Linfang Qian +3 位作者 Guangsong Chen Tong Lin Junfei Shi Shijie Zhou 《Defence Technology(防务技术)》 SCIE EI CAS CSCD 2024年第2期209-221,共13页
This paper proposed an efficient research method for high-dimensional uncertainty quantification of projectile motion in the barrel of a truck-mounted howitzer.Firstly,the dynamic model of projectile motion is establi... This paper proposed an efficient research method for high-dimensional uncertainty quantification of projectile motion in the barrel of a truck-mounted howitzer.Firstly,the dynamic model of projectile motion is established considering the flexible deformation of the barrel and the interaction between the projectile and the barrel.Subsequently,the accuracy of the dynamic model is verified based on the external ballistic projectile attitude test platform.Furthermore,the probability density evolution method(PDEM)is developed to high-dimensional uncertainty quantification of projectile motion.The engineering example highlights the results of the proposed method are consistent with the results obtained by the Monte Carlo Simulation(MCS).Finally,the influence of parameter uncertainty on the projectile disturbance at muzzle under different working conditions is analyzed.The results show that the disturbance of the pitch angular,pitch angular velocity and pitch angular of velocity decreases with the increase of launching angle,and the random parameter ranges of both the projectile and coupling model have similar influence on the disturbance of projectile angular motion at muzzle. 展开更多
关键词 Truck-mounted howitzer Projectile motion Uncertainty quantification Probability density evolution method
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Uncertainty quantification of mechanism motion based on coupled mechanism—motor dynamic model for ammunition delivery system 被引量:1
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作者 Jinsong Tang Linfang Qian +3 位作者 Longmiao Chen Guangsong Chen Mingming Wang Guangzu Zhou 《Defence Technology(防务技术)》 SCIE EI CAS CSCD 2024年第3期125-133,共9页
In this paper,a dynamic modeling method of motor driven electromechanical system is presented,and the uncertainty quantification of mechanism motion is investigated based on this method.The main contribution is to pro... In this paper,a dynamic modeling method of motor driven electromechanical system is presented,and the uncertainty quantification of mechanism motion is investigated based on this method.The main contribution is to propose a novel mechanism-motor coupling dynamic modeling method,in which the relationship between mechanism motion and motor rotation is established according to the geometric coordination of the system.The advantages of this include establishing intuitive coupling between the mechanism and motor,facilitating the discussion for the influence of both mechanical and electrical parameters on the mechanism,and enabling dynamic simulation with controller to take the randomness of the electric load into account.Dynamic simulation considering feedback control of ammunition delivery system is carried out,and the feasibility of the model is verified experimentally.Based on probability density evolution theory,we comprehensively discuss the effects of system parameters on mechanism motion from the perspective of uncertainty quantization.Our work can not only provide guidance for engineering design of ammunition delivery mechanism,but also provide theoretical support for modeling and uncertainty quantification research of mechatronics system. 展开更多
关键词 Ammunition delivery system Electromechanical coupling dynamics Uncertainty quantification Generalized probability density evolution
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Enhanced Cycling Stability of LiCoO2 at 4.6 V by Multi-component Doping
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作者 JIANG Weifeng YI Tingfeng 《材料导报》 EI CAS CSCD 北大核心 2019年第19期3175-3176,共2页
Fig.1 3D X-ray tomography reconstruction and element distribution in Ti,Mg and Al co-doped LiCoO2.3D spatial distributions of (a) Al,(b) Co and (c) Ti probed by fluorescence-yield scanning transmission X-ray microscop... Fig.1 3D X-ray tomography reconstruction and element distribution in Ti,Mg and Al co-doped LiCoO2.3D spatial distributions of (a) Al,(b) Co and (c) Ti probed by fluorescence-yield scanning transmission X-ray microscopy;elemental distributions of (d) Al,(e) Co and (f) Ti over the virtual x-z slice through the center of the particle;(g) identified and visualized subdomain formation Fig.2 (a) Comparison of cycling stabilities of Ti,Mg and Al co-doped LiCoO2 and pristine LiCoO2 half cells,charge-discharge profiles of (b) pristine LiCoO2 and (c) Ti,Mg and Al co-doped LiCoO2 half cells,(d) cycle stabilities of Ti,Mg and Al co-doped LiCoO2 and pristine LiCoO2 full batteries (graphite was used anode) and (e) discharge voltage of the full batteries and energy density of the both materials as a function of cycle number Layered lithium cobalt oxide (LiCoO2) with a theoretical capacity of 274 mAh·g^-1 has become a dominant cathode material for lithium-ion batteries of “3C” market (cellular phones,portable computers,camcorders)[1-2].Nevertheless,the actually attained capacity is merely about 140 mAh·g^-1 with a charge cut-off vol- tage of about 4.2 V (vs Li +/Li)[3]. 展开更多
关键词 multi-component DOPING CYCLING STABILITY
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Risk quantification in grade variability of gold deposits using sequential Gaussian simulation
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作者 Tayfun Y.Yunsel 《Journal of Central South University》 SCIE EI CAS 2012年第11期3244-3255,共12页
Risk quantification in grade is critical for mine design and planning.Grade uncertainty is assessed using multiple grade realizations,from geostatistical conditional simulations,which are effective to evaluate local o... Risk quantification in grade is critical for mine design and planning.Grade uncertainty is assessed using multiple grade realizations,from geostatistical conditional simulations,which are effective to evaluate local or global uncertainty by honouring spatial correlation structures.The sequential Gaussian conditional simulation was used to assess uncertainty of grade estimates and illustrate simulated models in Sivas gold deposit,Turkey.In situ variability and risk quantification of the gold grade were assessed by probabilistic approach based on the sequential Gaussian simulations to yield a series of conditional maps characterized by equally probable spatial distribution of the gold grade for the study area.The simulation results were validated by a number of tests such as descriptive statistics,histogram,variogram and contour map reproductions.The case study demonstrates the efficiency of the method in assessing risk associated with geological and engineering variable such as the gold grade variability and distribution.The simulated models can be incorporated into exploration,exploitation and scheduling of the gold deposit. 展开更多
关键词 sequential Gaussian simulation risk quantification probability distribution gold grade variability Sivas gold deposit
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Optimization of extended warranty cost for multi-component systems with economic dependence based on group maintenance
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作者 WANG Rongcai DONG Enzhi +1 位作者 CHENG Zhonghua WANG Qian 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2023年第2期396-407,共12页
During extended warranty(EW)period,maintenance events play a key role in controlling the product systems within normal operations.However,the modelling of failure process and maintenance optimization is complicated ow... During extended warranty(EW)period,maintenance events play a key role in controlling the product systems within normal operations.However,the modelling of failure process and maintenance optimization is complicated owing to the complex features of the product system,namely,components of the multi-component system are interdependent with each other in some form.For the purpose of optimizing the EW pricing decision of the multi-component system scientifically and rationally,taking the series multi-component system with economic dependence sold with EW policy as a research object,this paper optimizes the imperfect preventive maintenance(PM)strategy from the standpoint of EW cost.Taking into consideration adjusting the PM moments of the components in the system,a group maintenance model is developed,in which the system is repaired preventively in accordance with a specified PM base interval.In order to compare with the system EW cost before group maintenance,the system EW cost model before group maintenance is developed.Numerical example demonstrates that offering group maintenance programs can reduce EW cost of the system to a great extent,thereby reducing the EW price,which proves to be a win-win strategy to manufacturers and users. 展开更多
关键词 extended warranty(EW)cost multi-component system economic dependence preventive maintenance(PM) group maintenance
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Quantification of the expression of chitinolytic enzyme encoding genes ech30, ech42 and nag1 in Trichoderma atroviride P1 under varying growth conditions using a real-time RT-PCR assay
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作者 Jihong Liu Clarke Arne Tronsmo +1 位作者 Nicholas Clarkel Sonja Sletner Klemsdal 《浙江大学学报(农业与生命科学版)》 CAS CSCD 北大核心 2004年第4期428-428,共1页
The quantitative expression and the regulation of chitinase-encoding genes ech30, ech42 and nag1 in Trichoderma atroviride P1 under varying growth conditions were investigated using real-time RT-PCR, principle compone... The quantitative expression and the regulation of chitinase-encoding genes ech30, ech42 and nag1 in Trichoderma atroviride P1 under varying growth conditions were investigated using real-time RT-PCR, principle component and multivariate analyses. Twelve media combinations including 0.1% and 3% glucose as carbon source and no (0 mmol/L), low (10 mmol/L) and high (100 mmol/L) ammonium acetate as nitrogen source combined with or without colloidal chitin at 3 time intervals and 2 replications were applied to current study. The real-time RT-PCR analysis showed that the expression of ech30, ech42 and nag1 was regulated by the interaction of nitrogen, glucose and chitin under different growth conditions. The highest and earliest expressions of ech30 were induced by glucose and nitrogen starvation i.e. 0.1% glucose and 10 mmol/L ammonium acetate in the growth media. This was also the case for ech42 and nag1 but at a relatively low level. In contrast, high (3%) glucose and high (100 mmol/L) ammonium acetate concentrations repressed the expression of all the genes studied. These results were confirmed by principle component and multivariate analyses. The effect of chitin on ech30, ech42 and nag1 expression varied depending on the concentrations of glucose and ammonium acetate. 展开更多
关键词 基因 表达 生长条件 RT-PCR 木霉属 真菌
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Simulation Approach to Risk Quantification While Resources Estimation on Seismic and Log Data
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作者 A. G. Averbukh N. L. Ivanova 《岩性油气藏》 CSCD 2010年第F07期109-112,共4页
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农业生态补偿标准的量化方法研究与展望 被引量:3
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作者 乔玉辉 甄华杨 +1 位作者 冯旭 鞠鲤懋 《中国生态农业学报(中英文)》 北大核心 2025年第4期783-793,共11页
生态农业是实现农业可持续发展目标的核心路径之一,但是其发展面临着私人短期经济利益和公共长期生态效益的矛盾。农业生态补偿是化解这一矛盾的重要手段,农业生态补偿标准核算方法则是建立合理生态补偿机制的核心。目前,农业生态补偿... 生态农业是实现农业可持续发展目标的核心路径之一,但是其发展面临着私人短期经济利益和公共长期生态效益的矛盾。农业生态补偿是化解这一矛盾的重要手段,农业生态补偿标准核算方法则是建立合理生态补偿机制的核心。目前,农业生态补偿核算存在着农产品生态价值未被有效识别、核算体系不完善、服务价值与负服务价值未有机结合等核心科学问题。因此,当前亟须建立一套基于农业生态系统服务价值和负服务价值的农业生态补偿标准核算方法,以更好地支持农业生态补偿政策实施,并指导农业生态系统管理。本文提出了一个综合的农业生态补偿标准核算框架,主要包括生态保护成本、经济收益以及农业生态系统服务价值与负服务价值等,并详细介绍了农业生态系统服务的评估指标体系、价值化方法及负服务价值的核算方法,以此为农业生态补偿标准的确定提供理论支持与方法指导。本文从经济成本、相对生态价值和农业生态补偿情景分析等角度探讨了农业生态补偿标准的确定,旨在为各地制定科学合理的农业生态补偿标准提供参考。最后,本文展望了未来农业生态补偿标准核算研究的发展方向,包括生命周期综合生态系统服务价值评估、区域差异化管理以及学术研究与实践应用结合等方面,以期为未来农业生态补偿政策的制定与实施提供理论和方法支撑,促进农业生产与生态环境保护协调发展。 展开更多
关键词 生态补偿 生态农业 补偿标准 量化方法
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面向模型量化的安全性研究综述 被引量:1
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作者 陈晋音 曹志骐 +1 位作者 郑海斌 郑雅羽 《小型微型计算机系统》 北大核心 2025年第6期1473-1490,共18页
随着边缘智能设备的飞速发展,为了在资源受限的边缘端设备上部署参数和存储需求巨大的深度模型,模型压缩技术显得至关重要.现有的模型压缩主要包含剪枝、量化、知识蒸馏和低秩分解4类,量化凭借推理快、功耗低、存储少的优势,使它成为了... 随着边缘智能设备的飞速发展,为了在资源受限的边缘端设备上部署参数和存储需求巨大的深度模型,模型压缩技术显得至关重要.现有的模型压缩主要包含剪枝、量化、知识蒸馏和低秩分解4类,量化凭借推理快、功耗低、存储少的优势,使它成为了边缘端部署的常用技术.然而,已有的量化方法主要关注的是模型量化后的模型精度损失和内存占用情况,而忽略模型量化可能面临的安全性威胁.因此,针对模型量化的安全性研究显得尤为重要.本文首次针对模型量化的安全性问题展开分析,首先定义了模型量化的攻防理论,其次按照模型量化前和模型量化过程中两个阶段对量化攻击方法和量化防御方法进行分析归纳,整理了针对不同攻击任务进行的通用基准数据集与主要评价指标,最后探讨了模型量化的安全性研究及其应用,以及未来潜在研究方向,进一步推动模型量化的安全性研究发展和应用. 展开更多
关键词 模型量化 模型安全 对抗攻击 后门攻击 隐私窃取 公平性 模型防御
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水产养殖轨道式精准饲料投喂系统设计与试验 被引量:1
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作者 朱明 孙勇健 +3 位作者 雷翔 牛鹏基 赵振鹏 万鹏 《渔业现代化》 北大核心 2025年第2期78-89,共12页
针对水产养殖过程中饲料投喂劳动强度大、投喂不均匀、效率低等问题,设计了一款基于地轨的轨道式精准饲料投喂系统,整套系统集机械结构设计、自动控制系统、上位机监视投喂信息管理系统于一体。通过对系统的行走装置、料仓、下料装置、... 针对水产养殖过程中饲料投喂劳动强度大、投喂不均匀、效率低等问题,设计了一款基于地轨的轨道式精准饲料投喂系统,整套系统集机械结构设计、自动控制系统、上位机监视投喂信息管理系统于一体。通过对系统的行走装置、料仓、下料装置、称重装置等主要关键部件进行设计与理论分析,确定了系统的结构参数,基于SIEMENS S7-200 SMART PLC开发了自动控制系统,并以行驶速度、定位精度、投喂速度、投喂精度、饲料破碎率为试验指标进行饲料投喂试验。结果显示:系统运行稳定可靠,可以顺利自动启停,行驶速度为12.7 m/min,定位精度误差范围在39~58 mm,投喂速度为3.31 kg/min,投喂精度误差<0.63%,饲料破碎率低于1%。整个投喂全程自动化运行,上位机能够实时监视系统的行驶过程和投喂过程。研究表明,该系统提高了饲料利用率,降低了劳动成本和投饲成本,同时一体化的投饲设备能更好管理使用,为水产养殖自动化提供了可行方案。 展开更多
关键词 轨道式投饲系统 水产养殖 定位 定时定量 PLC 精准投喂
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一种用于接触网腕臂绝缘子破损大小检测的YOLOv7-OpenCV算法 被引量:1
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作者 张廷荣 段承成 +2 位作者 胡延文 张建鑫 范振华 《铁道科学与工程学报》 北大核心 2025年第4期1870-1882,共13页
针对接触网腕臂绝缘子采用传统机器加人工进行故障识别,难以实现绝缘子破损程度智能识别和即时监测的问题,提出一种YOLOv7-OpenCV算法以实现对腕臂绝缘子破损大小检测结果的即时量化。算法采用一种自适应直方图均衡化(AHE)策略以提升检... 针对接触网腕臂绝缘子采用传统机器加人工进行故障识别,难以实现绝缘子破损程度智能识别和即时监测的问题,提出一种YOLOv7-OpenCV算法以实现对腕臂绝缘子破损大小检测结果的即时量化。算法采用一种自适应直方图均衡化(AHE)策略以提升检测图像的局部对比度,同时将全局注意力机制(GAM)和全维度动态卷积(ODConv)模块引入到YOLOv7骨干网络中,加强网络对破损形状和大小等关键特征边缘信息的提取,提高模型对绝缘子破损目标的关注度和检测精度;在改进后的YOLOv7算法中集成OpenCV算法模块,通过滤波、边缘检测以及轮廓提取算法精确识别出破损轮廓,并实现对绝缘子破损大小的精准量化。通过对现场图像以及实验模拟条件下图像的检测性能结果对比分析,结果表明:所提出的改进算法对破损目标平均检测精度可达95.43%,相较于原YOLOv7算法提升了6.67%,提升了对破损识别的准确性;检测速率为41.3帧/s,满足实时监测对最小帧数的要求;对破损大小识别误差率较单一使用OPenCV平均降低了约10%,具有更高的检测精度与效率。研究所提出的针对腕臂绝缘子破损位置的精准定位与破损大小检测的实时量化算法,有利于提升牵引供电智能巡检水平。 展开更多
关键词 腕臂绝缘子 检测 YOLOv7 OPENCV 破损大小 量化
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用于非洲猪瘟病毒多靶标核酸检测的DNA假病毒制备及定量
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作者 邓俊花 李昊轩 +6 位作者 陈冬杰 吕继洲 王晶晶 张舟 袁向芬 魏方 吴绍强 《畜牧兽医学报》 北大核心 2025年第7期3555-3560,共6页
本研究旨在研制一种全程质控非洲猪瘟病毒(ASFV)核酸检测、避免质粒交叉污染的DNA假病毒。将ASFV B646L/CD2v/MGF505-1R/MGF360-12L 4种检测关键基因及EGFP基因共构建于杆状病毒的转移载体pFastBac^(TM)Dual中,转化形成重组Bacmid pFast... 本研究旨在研制一种全程质控非洲猪瘟病毒(ASFV)核酸检测、避免质粒交叉污染的DNA假病毒。将ASFV B646L/CD2v/MGF505-1R/MGF360-12L 4种检测关键基因及EGFP基因共构建于杆状病毒的转移载体pFastBac^(TM)Dual中,转化形成重组Bacmid pFastBac-EGFP-ASFV,转染至sf21细胞,包装产生ASFV重组杆状病毒。结果显示:通过荧光倒置显微镜观察,表达的EGFP蛋白绿色荧光信号指示成功获取AcMNPV-EGFP-ASFV病毒;重组杆状病毒基因组溯源分析,ASFV B646L/CD2v/MGF505-1R/MGF360-12L基因序列满足现行检测方法质控需求;ASFV质控品均匀、稳定,-20℃保存至少可稳定24个月。采用ddPCR定量为4.09×10^(3) copies·μL^(-1)。本研究非洲猪瘟病毒DNA假病毒的制备,为非洲猪瘟疫情监测提供了新的技术支撑。 展开更多
关键词 非洲猪瘟病毒 DNA假病毒 定量
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傅里叶变换红外光谱法测定吲哚美辛胶囊中滑石粉的含量
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作者 曹桂红 刘璐 +2 位作者 黄春青 许波 王璐 《理化检验(化学分册)》 北大核心 2025年第11期1329-1333,共5页
为实现药品制剂中滑石粉含量的准确测定,提出了题示方法。取含滑石粉约5 mg的吲哚美辛胶囊内容物,置于已于450℃恒重的瓷坩埚内,在500℃加热板上碳化30 min,再于450℃炽灼至恒重。取约一半质量的残渣和100 mg溴化钾,在玛瑙研钵中研磨5 m... 为实现药品制剂中滑石粉含量的准确测定,提出了题示方法。取含滑石粉约5 mg的吲哚美辛胶囊内容物,置于已于450℃恒重的瓷坩埚内,在500℃加热板上碳化30 min,再于450℃炽灼至恒重。取约一半质量的残渣和100 mg溴化钾,在玛瑙研钵中研磨5 min,分取约50 mg,在压力40 kN下压片3 min,采用傅里叶变换红外光谱仪测量(669±2)cm^(-1)处滑石粉特征吸收峰的吸光度,并根据吸光度和滑石粉质量分数间的线性定量关系计算样品中滑石粉的含量。结果显示:滑石粉的线性范围为0.5%~3.5%,检出限为0.05%;对滑石粉对照品片和样片进行精密度试验,吸光度的相对标准偏差(n=6)均不大于4.0%;按照标准加入法进行回收试验,回收率为101%~110%。方法用于7个企业87批吲哚美辛胶囊样品的分析,在4个企业生产的样品中检出了滑石粉,检出量为1.90~12.70 mg·粒^(-1),与企业提供的处方量不符,且在3个企业生产的样品中存在明显的批次间差异。 展开更多
关键词 傅里叶变换红外光谱法 吲哚美辛胶囊 滑石粉 定量
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