Taking annual Heracl'eum moellendorffii Hance as the material,this research studied the epidemic dynamics of H.moellendorffii Hance powdery mildew and plant growth by using the interactive methods of soil moisture...Taking annual Heracl'eum moellendorffii Hance as the material,this research studied the epidemic dynamics of H.moellendorffii Hance powdery mildew and plant growth by using the interactive methods of soil moisture and soil organic matter.The results showed that the interaction of higher soil moisture content(60%-80%)and soil organic matter content(75-100 g•kg^(-1))had few diseases,dosease index and area under disease progression curve(AUDPC)compared with those of other treatments,thus could effectivley control powdery mildew disease.Moreover,higher soil moisture and organic matter content increased the yield,contents of soluble sugar,soluble protein,vitamin C and coumarin in H.moellendorffii Hance.展开更多
1 mL of sporangial suspensions (5 x 10 5spporangia per milliliter) of Pseudoperonospora cubensis was droplet-inoculated on the surface of the second leaf of the plant grown in greenhouse ( inducing inoculatoin), then ...1 mL of sporangial suspensions (5 x 10 5spporangia per milliliter) of Pseudoperonospora cubensis was droplet-inoculated on the surface of the second leaf of the plant grown in greenhouse ( inducing inoculatoin), then the lower surfaces of the third, the forth and the fifth leaves were uniformly sprayed with inoculum of the same fungi (5 x 10 4sporangia per milliliter, about 5 mL per plant) every 3 days interval (challenge inoculation). Plants were moistened at 18- 22 C for 18 h, then kept at room temperature (24 - 28 C) and supplemented with cool-white fluorescent lights. All three challenge leaves were collected after 7 days of challenge to measure the amount of sporulation and area of necrosis. Plants prior inoculated with P. cubensis were protected 38% (based on the area of necrosis) against disaesc caused by subsequent foliar challenge with the pathogen. Protective action was about 12% after 3 days, and maintained this level until 9 days, suddenly reached 34% after 12 days, and came to a maximum after 15 days, then dropped down slowly.展开更多
Powdery mildew is one of the most serious diseases of wheat in China. In this paper,bulked segregant analysis (BSA) was used to search for randomly amplified polymorphic DNA (RAPD) markers linked to the Pm12 gene,whic...Powdery mildew is one of the most serious diseases of wheat in China. In this paper,bulked segregant analysis (BSA) was used to search for randomly amplified polymorphic DNA (RAPD) markers linked to the Pm12 gene,which confers resistance to the powdery mildew in wheat. 200 decamer primers were screened and one RAPD marker (S107 1900 ) was identified to be linked to Pm12 in coupling phase,and their genetic distance is 11.98± 4.00cM. This marker can be used for marker-assisted selection in wheat breeding for the identification or pyramiding of Pm12 with other resistance genes.展开更多
基金Supported by the National Key Research and Development Program of China(2016YFC0500307-06)。
文摘Taking annual Heracl'eum moellendorffii Hance as the material,this research studied the epidemic dynamics of H.moellendorffii Hance powdery mildew and plant growth by using the interactive methods of soil moisture and soil organic matter.The results showed that the interaction of higher soil moisture content(60%-80%)and soil organic matter content(75-100 g•kg^(-1))had few diseases,dosease index and area under disease progression curve(AUDPC)compared with those of other treatments,thus could effectivley control powdery mildew disease.Moreover,higher soil moisture and organic matter content increased the yield,contents of soluble sugar,soluble protein,vitamin C and coumarin in H.moellendorffii Hance.
文摘1 mL of sporangial suspensions (5 x 10 5spporangia per milliliter) of Pseudoperonospora cubensis was droplet-inoculated on the surface of the second leaf of the plant grown in greenhouse ( inducing inoculatoin), then the lower surfaces of the third, the forth and the fifth leaves were uniformly sprayed with inoculum of the same fungi (5 x 10 4sporangia per milliliter, about 5 mL per plant) every 3 days interval (challenge inoculation). Plants were moistened at 18- 22 C for 18 h, then kept at room temperature (24 - 28 C) and supplemented with cool-white fluorescent lights. All three challenge leaves were collected after 7 days of challenge to measure the amount of sporulation and area of necrosis. Plants prior inoculated with P. cubensis were protected 38% (based on the area of necrosis) against disaesc caused by subsequent foliar challenge with the pathogen. Protective action was about 12% after 3 days, and maintained this level until 9 days, suddenly reached 34% after 12 days, and came to a maximum after 15 days, then dropped down slowly.
文摘Powdery mildew is one of the most serious diseases of wheat in China. In this paper,bulked segregant analysis (BSA) was used to search for randomly amplified polymorphic DNA (RAPD) markers linked to the Pm12 gene,which confers resistance to the powdery mildew in wheat. 200 decamer primers were screened and one RAPD marker (S107 1900 ) was identified to be linked to Pm12 in coupling phase,and their genetic distance is 11.98± 4.00cM. This marker can be used for marker-assisted selection in wheat breeding for the identification or pyramiding of Pm12 with other resistance genes.
