Utilizing a reliable, reproducible arterial inversiongraft thrombosis model, comparison of intraarterial VSwith intravenous heparin delivery in maintaining micro-vascular patency has been finished, concentrated on the...Utilizing a reliable, reproducible arterial inversiongraft thrombosis model, comparison of intraarterial VSwith intravenous heparin delivery in maintaining micro-vascular patency has been finished, concentrated on thestudy of the effective mechanism of heparin. Vesselpatency, complication rate and representative electromi-croscopy section of the AIG were studied. Patency inthe intraarterial group is 90% (26/29) as compared to50% (12/26) in the intravenous delivery group(P【0.05).Scanning electromicroscopy showed a significant absenceof dense fibrin deposition and decrease in the numberof aggregated platelets in the intra-arterial perfusedAIG. The results of the present study indicate that thesmall dose of heparin through the artery is still effectivein maintaining the vessel pateney. Furthermore, in alarge, carefully controlled series, the complication ratewas low.展开更多
OBJECTIVE To investigate the regulatory effects of icariin(ICA)on cardiac micro⁃vascular endothelial cells(CMEC)after oxygenglucose deprivation reperfusion(OGD/R)injury.METHODS CMEC were subjected to OGD/R treatment t...OBJECTIVE To investigate the regulatory effects of icariin(ICA)on cardiac micro⁃vascular endothelial cells(CMEC)after oxygenglucose deprivation reperfusion(OGD/R)injury.METHODS CMEC were subjected to OGD/R treatment to construct a myocardial ischemiareperfusion model,and were divided into normal,model,low(10μmol·L^(-1)),medium(20μmol·L^(-1))and high(40μmol·L^(-1))ICA group,and high ICA+inhibitor group(40μmol·L^(-1)+20 nmol·L^(-1)).CCK-8 assay was used to assess the protective ability of ICA against CMEC,and cell migration assay and tube-formation assay were used to detect the migration and generation ability of CMEC.The TCMSP database,Swiss-Target database and literature mining methods were used to col⁃lect ICA-related targets,the GeneCards data⁃base was used to collect target genes related to myocardial ischemia/reperfusion,and Cytoscape 3.8.0 software was used to construct a"drug-tar⁃get-disease"network.The potential targets were imported into STRING 11.5 database to obtain the PPI network.GO and KEGG enrichment analyses were performed on the potential targets using the DAVID database.Molecular docking was performed using AutoDock-vina 1.1.2 soft⁃ware.Western blot detected the expression of related proteins.RESULTS After CMEC was subjected to OGD/R treatment,ICA had a protec⁃tive effect at 10^(-1)60μmol·L^(-1);the results of the cell migration assay showed that each group of ICA could promote the migratory effect of CMEC(P<0.01,P<0.01);and the results of tube-for⁃mation assay showed that each group of ICA could significantly promote the generation of branches(P<0.01)and the capillary length exten⁃sion(P<0.05).Network pharmacology collected a total of 23 ICA action targets,1500 disease tar⁃gets and 12 key targets.GO function enrichment analysis found 85 results.KEGG pathway enrich⁃ment analysis found 53 results,involving AGERAGE signaling pathway,sphingolipid signaling pathway and VEGF signaling pathway.Molecu⁃lar docking results showed that ICA had better binding with core targets PRKCB,PRKCA and PTGS2.Western blot results showed that ICA could regulate the expression of PRKCB,PRKCA and PTGS2 proteins.The results of cell migra⁃tion assay,tube-formation assay and protein expression were reversed after addition of PKC inhibitor.CONCLUSION The potential mecha⁃nism of action of ICA against myocardial isch⁃emia-reperfusion injury may be related to the reg⁃ulation of processes such as CMEC migration and angiogenesis,and it functions through the key target gene PKC.展开更多
微血管网络在机体生理功能的实现中起着至关重要的作用,其生成研究为受损微血管网络的修复提供了潜在的治疗方向。目前,已有研究完成了虚拟异质性微血管树的生成,但由于显微图像无法清晰呈现毛细血管分布,开发符合真实生长规律的毛细血...微血管网络在机体生理功能的实现中起着至关重要的作用,其生成研究为受损微血管网络的修复提供了潜在的治疗方向。目前,已有研究完成了虚拟异质性微血管树的生成,但由于显微图像无法清晰呈现毛细血管分布,开发符合真实生长规律的毛细血管生长算法成为微血管网络生成领域的关键挑战。本研究提出了一种基于异质性微血管树的毛细血管生长算法。研究首先获取3个大鼠肠系膜微血管网络结构,剔除网络中的毛细血管,并分别提取独立的微动脉树和微静脉树;随后,设计并实现了以末端出芽方式进行毛细血管生长的算法,结合归巢机制引导毛细血管生长,模拟其生理特性;最终,通过生理性修剪和优化,构建出虚拟异质性微血管网络。实验对于每个大鼠肠系膜微血管网络记录了30次虚拟微血管网络生成,并对生成网络与真实网络的血管分形维数(FD)和血管密度(VD)进行对比分析。结果显示,生成网络与真实网络的FD和VD均高度相似(FD:1.548±0.025 vs 1.565±0.005,P>0.05,VD:0.060±0.004 vs 0.059±0.003,P>0.05),验证了算法在模拟毛细血管生长上的合理性。此外,通过分形维数空间分布观察,生成网络与真实网络在局部复杂度分布上基本一致。本研究提出的算法为基于异质性微血管树的虚拟微循环网络生成提供了新思路。展开更多
文摘Utilizing a reliable, reproducible arterial inversiongraft thrombosis model, comparison of intraarterial VSwith intravenous heparin delivery in maintaining micro-vascular patency has been finished, concentrated on thestudy of the effective mechanism of heparin. Vesselpatency, complication rate and representative electromi-croscopy section of the AIG were studied. Patency inthe intraarterial group is 90% (26/29) as compared to50% (12/26) in the intravenous delivery group(P【0.05).Scanning electromicroscopy showed a significant absenceof dense fibrin deposition and decrease in the numberof aggregated platelets in the intra-arterial perfusedAIG. The results of the present study indicate that thesmall dose of heparin through the artery is still effectivein maintaining the vessel pateney. Furthermore, in alarge, carefully controlled series, the complication ratewas low.
基金National Natural Science Foundation of China(82030124)National Natural Science Foundation of China(82174015)Science and Technology Innovation Project of China Academy of Traditional Chinese Medicine(CI2021A04609)。
文摘OBJECTIVE To investigate the regulatory effects of icariin(ICA)on cardiac micro⁃vascular endothelial cells(CMEC)after oxygenglucose deprivation reperfusion(OGD/R)injury.METHODS CMEC were subjected to OGD/R treatment to construct a myocardial ischemiareperfusion model,and were divided into normal,model,low(10μmol·L^(-1)),medium(20μmol·L^(-1))and high(40μmol·L^(-1))ICA group,and high ICA+inhibitor group(40μmol·L^(-1)+20 nmol·L^(-1)).CCK-8 assay was used to assess the protective ability of ICA against CMEC,and cell migration assay and tube-formation assay were used to detect the migration and generation ability of CMEC.The TCMSP database,Swiss-Target database and literature mining methods were used to col⁃lect ICA-related targets,the GeneCards data⁃base was used to collect target genes related to myocardial ischemia/reperfusion,and Cytoscape 3.8.0 software was used to construct a"drug-tar⁃get-disease"network.The potential targets were imported into STRING 11.5 database to obtain the PPI network.GO and KEGG enrichment analyses were performed on the potential targets using the DAVID database.Molecular docking was performed using AutoDock-vina 1.1.2 soft⁃ware.Western blot detected the expression of related proteins.RESULTS After CMEC was subjected to OGD/R treatment,ICA had a protec⁃tive effect at 10^(-1)60μmol·L^(-1);the results of the cell migration assay showed that each group of ICA could promote the migratory effect of CMEC(P<0.01,P<0.01);and the results of tube-for⁃mation assay showed that each group of ICA could significantly promote the generation of branches(P<0.01)and the capillary length exten⁃sion(P<0.05).Network pharmacology collected a total of 23 ICA action targets,1500 disease tar⁃gets and 12 key targets.GO function enrichment analysis found 85 results.KEGG pathway enrich⁃ment analysis found 53 results,involving AGERAGE signaling pathway,sphingolipid signaling pathway and VEGF signaling pathway.Molecu⁃lar docking results showed that ICA had better binding with core targets PRKCB,PRKCA and PTGS2.Western blot results showed that ICA could regulate the expression of PRKCB,PRKCA and PTGS2 proteins.The results of cell migra⁃tion assay,tube-formation assay and protein expression were reversed after addition of PKC inhibitor.CONCLUSION The potential mecha⁃nism of action of ICA against myocardial isch⁃emia-reperfusion injury may be related to the reg⁃ulation of processes such as CMEC migration and angiogenesis,and it functions through the key target gene PKC.
文摘微血管网络在机体生理功能的实现中起着至关重要的作用,其生成研究为受损微血管网络的修复提供了潜在的治疗方向。目前,已有研究完成了虚拟异质性微血管树的生成,但由于显微图像无法清晰呈现毛细血管分布,开发符合真实生长规律的毛细血管生长算法成为微血管网络生成领域的关键挑战。本研究提出了一种基于异质性微血管树的毛细血管生长算法。研究首先获取3个大鼠肠系膜微血管网络结构,剔除网络中的毛细血管,并分别提取独立的微动脉树和微静脉树;随后,设计并实现了以末端出芽方式进行毛细血管生长的算法,结合归巢机制引导毛细血管生长,模拟其生理特性;最终,通过生理性修剪和优化,构建出虚拟异质性微血管网络。实验对于每个大鼠肠系膜微血管网络记录了30次虚拟微血管网络生成,并对生成网络与真实网络的血管分形维数(FD)和血管密度(VD)进行对比分析。结果显示,生成网络与真实网络的FD和VD均高度相似(FD:1.548±0.025 vs 1.565±0.005,P>0.05,VD:0.060±0.004 vs 0.059±0.003,P>0.05),验证了算法在模拟毛细血管生长上的合理性。此外,通过分形维数空间分布观察,生成网络与真实网络在局部复杂度分布上基本一致。本研究提出的算法为基于异质性微血管树的虚拟微循环网络生成提供了新思路。
