MicroRNAs (miRNAs) are a group of regulatory RNAs that regulate gene expression post-transcriptionally by the degradation or translational inhibition of their target messenger RNAs (mRNAs). Regulation is accomplis...MicroRNAs (miRNAs) are a group of regulatory RNAs that regulate gene expression post-transcriptionally by the degradation or translational inhibition of their target messenger RNAs (mRNAs). Regulation is accomplished when the 22-25 nucleotide miRNAs bind to complementary sequences in the 3'-untranslated regions (UTR). One barrier to miRNA research is to find target genes. Although computational target predictions have shed light on important aspects of microRNA target recognition, questions remain concerning the rates of false positives. In addition, we do not completely understand how microRNAs can recognize and regulate their targets. As such, experimental positive predictions and allow for an unbiased stu ap dy proaches are required, which can reflect in vivo processes, eliminating false of microRNA target recognition. In this review, we summarized experimental approaches that have been described for the identification and validation of mRNA targets associated with specific miRNAs.展开更多
基金Supported by the National Natural Science Foundation of China (30570990, 30471059, 31171578)the "863" project (2008AA10Z153)+2 种基金the Key Research Plan of Heilongjiang Province (GA06B103-3)the Innovation Research Group of NEAU (CXT004)the Research Fund for the Doctoral Program of Higher Education of China (20102325120002)
文摘MicroRNAs (miRNAs) are a group of regulatory RNAs that regulate gene expression post-transcriptionally by the degradation or translational inhibition of their target messenger RNAs (mRNAs). Regulation is accomplished when the 22-25 nucleotide miRNAs bind to complementary sequences in the 3'-untranslated regions (UTR). One barrier to miRNA research is to find target genes. Although computational target predictions have shed light on important aspects of microRNA target recognition, questions remain concerning the rates of false positives. In addition, we do not completely understand how microRNAs can recognize and regulate their targets. As such, experimental positive predictions and allow for an unbiased stu ap dy proaches are required, which can reflect in vivo processes, eliminating false of microRNA target recognition. In this review, we summarized experimental approaches that have been described for the identification and validation of mRNA targets associated with specific miRNAs.