Previous studies have suggested that polypeptides extracted from milk, soybean, fish, eggs, and meat possess potential anti-inflammatory effects. To date, few studies have reported the anti-inflammatory function of st...Previous studies have suggested that polypeptides extracted from milk, soybean, fish, eggs, and meat possess potential anti-inflammatory effects. To date, few studies have reported the anti-inflammatory function of sturgeon peptides and their underlying mechanisms are unknown. The current study was therefore to determine the anti-inflammatory potential of sturgeon peptides with lipopolysaccharide (LPS)-induced RAW264.7 inflammatory model. Pepsin hydrolysate (PeH) was purified by ultrafiltration and Sephadex G-15 gel filtration chromatography. PeH significantly reduced the inflammatory mediator (NO) and inflammatory cytokines (IL-6, TNF-α and IL-1β) expression in a dose-dependent manner. Moreover, the purified sturgeon peptide (F2) possessed strong antioxidant potential and effectively inhibited DPPH and ABTS free radicals. F2 significantly suppressed the expression of MAPK, IκBα, and NF-κB p65, indicating that F2 exerted anti-inflammatory influence by the inhibition of MAPK and NF-κB pathways.展开更多
目的:探究叉分蓼多糖(Polygonum divaricatum L. polysaccharides,PSPDL)体外抗炎活性及其作用机制。方法:从叉分蓼中提取多糖,采用高效凝胶渗透色谱、高效液相色谱、傅里叶变换红外光谱、X射线衍射、核磁共振波谱技术对其结构进行表征...目的:探究叉分蓼多糖(Polygonum divaricatum L. polysaccharides,PSPDL)体外抗炎活性及其作用机制。方法:从叉分蓼中提取多糖,采用高效凝胶渗透色谱、高效液相色谱、傅里叶变换红外光谱、X射线衍射、核磁共振波谱技术对其结构进行表征,并通过脂多糖诱导RAW264.7细胞炎症模型评估其体外抗炎活性及其作用机制。结果:PSPDL的重均分子质量为59.475 kDa,主要由甘露糖、鼠李糖、葡萄糖醛酸、半乳糖醛酸、葡萄糖、半乳糖、阿拉伯糖组成,其物质的量比为1.69∶4.95∶1.04∶21.79∶19.01∶31.68∶19.84,是含有(1→4)-α-D-Glcp的α-吡喃型多糖;能够抑制炎症因子的释放及其相关mRNA的表达,改善氧化应激,下调p38、p-p38、IκB-α、p65和p-p65蛋白的表达水平。结论:PSDPL具有抗炎活性,其作用机制可能与调节炎症相关mRNA表达,调控丝裂原活化蛋白激酶/核转录因子κB信号通路相关,本研究结果可为PSPDL资源的开发与应用提供科学依据。展开更多
多种真菌多糖近年来因其免疫调节活性成为保健食品领域研究的热点。本实验以食药用真菌蛹虫草提取物蛹虫草多糖(Cordyceps militaris polysaccharides,CMP)为研究材料,初步探究CMP对小鼠巨噬细胞RAW264.7免疫活性的调节机制。噻唑蓝实...多种真菌多糖近年来因其免疫调节活性成为保健食品领域研究的热点。本实验以食药用真菌蛹虫草提取物蛹虫草多糖(Cordyceps militaris polysaccharides,CMP)为研究材料,初步探究CMP对小鼠巨噬细胞RAW264.7免疫活性的调节机制。噻唑蓝实验结果显示CMP无细胞毒性,且100、200μg/mL CMP可以明显增强RAW264.7细胞活性;中性红法、Griess法和酶联免疫吸附检测结果表明25～200μg/mL的CMP以剂量依赖方式增强RAW264.7细胞吞噬活性并增加一氧化氮(nitric oxide,NO)和白介素-1β(interleukin-1β,IL-1β)分泌,肿瘤坏死因子-α(tumor necrosisfactor-α,TNF-α)的分泌呈先升高后降低的趋势,在100μg/mL时达到最高值;抑制剂中和实验结果显示当Toll样受体4(Toll-like receptors 4,TLR4)和甘露糖受体(mannose receptor,MR)受到抑制时,CMP诱导的RAW264.7免疫反应显著降低,这表明TLR4和MR均为CMP激活巨噬细胞的受体;此外,Western blot结果显示丝裂原活化蛋白激酶(mitogen-activated protein kinases,MAPK)信号通路也参与CMP诱导的RAW264.7细胞分泌NO、TNF-α和IL-1β。表明TLR4和MR/MAPK信号传导途径在CMP诱导巨噬细胞RAW264.7产生免疫应答时发挥了重要作用。展开更多
文摘Previous studies have suggested that polypeptides extracted from milk, soybean, fish, eggs, and meat possess potential anti-inflammatory effects. To date, few studies have reported the anti-inflammatory function of sturgeon peptides and their underlying mechanisms are unknown. The current study was therefore to determine the anti-inflammatory potential of sturgeon peptides with lipopolysaccharide (LPS)-induced RAW264.7 inflammatory model. Pepsin hydrolysate (PeH) was purified by ultrafiltration and Sephadex G-15 gel filtration chromatography. PeH significantly reduced the inflammatory mediator (NO) and inflammatory cytokines (IL-6, TNF-α and IL-1β) expression in a dose-dependent manner. Moreover, the purified sturgeon peptide (F2) possessed strong antioxidant potential and effectively inhibited DPPH and ABTS free radicals. F2 significantly suppressed the expression of MAPK, IκBα, and NF-κB p65, indicating that F2 exerted anti-inflammatory influence by the inhibition of MAPK and NF-κB pathways.
文摘为解析槲皮万寿菊素、槲皮素与叶黄素单独处理以及联合处理对急性肺损伤的作用机制,以脂多糖诱导构建RAW264.7细胞炎症模型,以一氧化氮(nitric oxide,NO)相对含量为评价指标,采用联合指数法确定槲皮万寿菊素与叶黄素以及槲皮素与叶黄素的最佳复配比例;分析比较槲皮万寿菊素、槲皮素与叶黄素单独及联合处理对RAW264.7细胞中炎症因子(肿瘤坏死因子-α、白细胞介素(interleukin,IL)-1β、IL-6)含量、丙二醛含量、超氧化物歧化酶活性以及谷胱甘肽过氧化物酶活性的影响;采用免疫印迹法测定核因子κB(nuclear factor kappa-B,NF-κB)信号通路中p65、p50以及沉默信息调节因子1(silent information regulator 1,SIRT1)、核因子E2相关因子2(nuclear factor-erythroid 2 related factor 2,Nrf2)和NOD样受体热蛋白结构域相关蛋白3(NOD-like receptor thermal protein domain associated protein 3,NLRP3)的相对表达量。结果表明,槲皮万寿菊素与叶黄素高剂量3∶1(30μg/mL+10μg/mL)复配能够最大程度降低RAW264.7细胞中的NO相对含量。二者单独及联合作用均能通过降低炎症因子、丙二醛含量,提高超氧化物歧化酶和谷胱甘肽还原酶活性,下调NF-κB p65、p50以及NLRP3表达水平并上调SIRT1、Nrf2蛋白相对表达量发挥改善急性肺损伤的作用,且联合处理效果优于单独处理组。
文摘目的:探究叉分蓼多糖(Polygonum divaricatum L. polysaccharides,PSPDL)体外抗炎活性及其作用机制。方法:从叉分蓼中提取多糖,采用高效凝胶渗透色谱、高效液相色谱、傅里叶变换红外光谱、X射线衍射、核磁共振波谱技术对其结构进行表征,并通过脂多糖诱导RAW264.7细胞炎症模型评估其体外抗炎活性及其作用机制。结果:PSPDL的重均分子质量为59.475 kDa,主要由甘露糖、鼠李糖、葡萄糖醛酸、半乳糖醛酸、葡萄糖、半乳糖、阿拉伯糖组成,其物质的量比为1.69∶4.95∶1.04∶21.79∶19.01∶31.68∶19.84,是含有(1→4)-α-D-Glcp的α-吡喃型多糖;能够抑制炎症因子的释放及其相关mRNA的表达,改善氧化应激,下调p38、p-p38、IκB-α、p65和p-p65蛋白的表达水平。结论:PSDPL具有抗炎活性,其作用机制可能与调节炎症相关mRNA表达,调控丝裂原活化蛋白激酶/核转录因子κB信号通路相关,本研究结果可为PSPDL资源的开发与应用提供科学依据。
