With the help of adaptive optics （AO） technology, cellular level imaging of living human retina can be achieved. Aiming to reduce distressing feelings and to avoid potential drug induced diseases, we attempted to im...With the help of adaptive optics （AO） technology, cellular level imaging of living human retina can be achieved. Aiming to reduce distressing feelings and to avoid potential drug induced diseases, we attempted to image retina with dilated pupil and froze accommodation without drugs. An optimized liquid crystal adaptive optics camera was adopted for retinal imaging. A novel eye stared system was used for stimulating accommodation and fixating imaging area. Illumination sources and imaging camera kept linkage for focusing and imaging different layers. Four subjects with diverse degree of myopia were imaged. Based on the optical properties of the human eye, the eye stared system reduced the defocus to less than the typical ocular depth of focus. In this way, the illumination light can be projected on certain retina layer precisely. Since that the defocus had been compensated by the eye stared system, the adopted 512 × 512 liquid crystal spatial light modulator （LC-SLM） corrector provided the crucial spatial fidelity to fully compensate high-order aberrations. The Strehl ratio of a subject with -8 diopter myopia was improved to 0.78, which was nearly close to diffraction-limited imaging. By finely adjusting the axial displacement of illumination sources and imaging camera, cone photoreceptors, blood vessels and nerve fiber layer were clearly imaged successfully.展开更多
In order to obtain a clear image of the retina of model eye, an adaptive optics system used to correct the wave-front error is introduced in this paper. The spatial light modulator that we use here is a liquid crystal...In order to obtain a clear image of the retina of model eye, an adaptive optics system used to correct the wave-front error is introduced in this paper. The spatial light modulator that we use here is a liquid crystal on a silicon device instead of a conversional deformable mirror. A paper with carbon granule is used to simulate the retina of human eye. The pupil size of the model eye is adjustable （3-7mm）. A Shack-Hartman wave-front sensor is used to detect the wave-front aberration. With this construction, a value of peak-to-valley is achieved to be 0.086 A, where A is wavelength. The modulation transfer functions before and after corrections are compared. And the resolution of this system after correction （691p/m） is very close to the diffraction limit resolution. The carbon granule on the white paper which has a size of 4.7μm is seen clearly. The size of the retina cell is between 4 and 10 μm. So this system has an ability to image the human eye＇s retina.展开更多
Even in the early stage,endocrine metabolism disease may lead to micro aneurysms in retinal capillaries whose diameters are less than 10 μm.However,the fundus cameras used in clinic diagnosis can only obtain images o...Even in the early stage,endocrine metabolism disease may lead to micro aneurysms in retinal capillaries whose diameters are less than 10 μm.However,the fundus cameras used in clinic diagnosis can only obtain images of vessels larger than 20 μm in diameter.The human retina is a thin and multiple layer tissue,and the layer of capillaries less than10 μm in diameter only exists in the inner nuclear layer.The layer thickness of capillaries less than 10 μm in diameter is about 40 μm and the distance range to rod＆cone cell surface is tens of micrometers,which varies from person to person.Therefore,determining reasonable capillary layer（CL） position in different human eyes is very difficult.In this paper,we propose a method to determine the position of retinal CL based on the rod＆cone cell layer.The public positions of CL are recognized with 15 subjects from 40 to 59 years old,and the imaging planes of CL are calculated by the effective focal length of the human eye.High resolution retinal capillary imaging results obtained from 17 subjects with a liquid crystal adaptive optics system（LCAOS） validate our method.All of the subjects＇ CLs have public positions from 127 μm to 147 μm from the rod＆cone cell layer,which is influenced by the depth of focus.展开更多
This paper proposes a new Zernike modal gray map reconstruction algorithm used in the nematic liquid crystal adaptive optics system. Firstly, the new modal algorithm is described. Secondly, a single loop correction ex...This paper proposes a new Zernike modal gray map reconstruction algorithm used in the nematic liquid crystal adaptive optics system. Firstly, the new modal algorithm is described. Secondly, a single loop correction experiment was conducted, and it showed that the modal method has a higher precision in gray map reconstruction than the widely used slope method. Finally, the contrast close-loop correction experiment was conducted to correct static aberration in the laboratory. The experimental results showed that the average peak to valley （PV） and root mean square （RMS） of the wavefront corrected by mode method were reduced from 2.501A （λ= 633 nm） and 0.610A to 0.0334λ and 0.00845A, respectively. The corrected PV and RMS were much smaller than those of 0.173A and 0.048A by slope method. The Strehl ratio and modulation transfer function of the system corrected by mode method were much closer to diffraction limit than with slope method. These results indicate that the mode method can take good advantage of the large number of pixels of the liquid crystal corrector to realize high correction precision.展开更多
基金supported by the National Natural Science Foundation of China(Grant Nos.60736042,1174274,and 1174279)the Plan for Scientific and Technology Development of Suzhou,China(Grant No.ZXS201001)
文摘With the help of adaptive optics （AO） technology, cellular level imaging of living human retina can be achieved. Aiming to reduce distressing feelings and to avoid potential drug induced diseases, we attempted to image retina with dilated pupil and froze accommodation without drugs. An optimized liquid crystal adaptive optics camera was adopted for retinal imaging. A novel eye stared system was used for stimulating accommodation and fixating imaging area. Illumination sources and imaging camera kept linkage for focusing and imaging different layers. Four subjects with diverse degree of myopia were imaged. Based on the optical properties of the human eye, the eye stared system reduced the defocus to less than the typical ocular depth of focus. In this way, the illumination light can be projected on certain retina layer precisely. Since that the defocus had been compensated by the eye stared system, the adopted 512 × 512 liquid crystal spatial light modulator （LC-SLM） corrector provided the crucial spatial fidelity to fully compensate high-order aberrations. The Strehl ratio of a subject with -8 diopter myopia was improved to 0.78, which was nearly close to diffraction-limited imaging. By finely adjusting the axial displacement of illumination sources and imaging camera, cone photoreceptors, blood vessels and nerve fiber layer were clearly imaged successfully.
基金Project supported by the National Natural Science Foundation of China (Grant Nos 60578035, 50473040 and 60736042)the Science Foundation of Jilin Province, China (Grant Nos 20050520 and 20050321-2)
文摘In order to obtain a clear image of the retina of model eye, an adaptive optics system used to correct the wave-front error is introduced in this paper. The spatial light modulator that we use here is a liquid crystal on a silicon device instead of a conversional deformable mirror. A paper with carbon granule is used to simulate the retina of human eye. The pupil size of the model eye is adjustable （3-7mm）. A Shack-Hartman wave-front sensor is used to detect the wave-front aberration. With this construction, a value of peak-to-valley is achieved to be 0.086 A, where A is wavelength. The modulation transfer functions before and after corrections are compared. And the resolution of this system after correction （691p/m） is very close to the diffraction limit resolution. The carbon granule on the white paper which has a size of 4.7μm is seen clearly. The size of the retina cell is between 4 and 10 μm. So this system has an ability to image the human eye＇s retina.
基金Project supported by the National Natural Science Foundation of China(Grant Nos.11174274,11174279,61205021,11204299,61475152,and 61405194)
文摘Even in the early stage,endocrine metabolism disease may lead to micro aneurysms in retinal capillaries whose diameters are less than 10 μm.However,the fundus cameras used in clinic diagnosis can only obtain images of vessels larger than 20 μm in diameter.The human retina is a thin and multiple layer tissue,and the layer of capillaries less than10 μm in diameter only exists in the inner nuclear layer.The layer thickness of capillaries less than 10 μm in diameter is about 40 μm and the distance range to rod＆cone cell surface is tens of micrometers,which varies from person to person.Therefore,determining reasonable capillary layer（CL） position in different human eyes is very difficult.In this paper,we propose a method to determine the position of retinal CL based on the rod＆cone cell layer.The public positions of CL are recognized with 15 subjects from 40 to 59 years old,and the imaging planes of CL are calculated by the effective focal length of the human eye.High resolution retinal capillary imaging results obtained from 17 subjects with a liquid crystal adaptive optics system（LCAOS） validate our method.All of the subjects＇ CLs have public positions from 127 μm to 147 μm from the rod＆cone cell layer,which is influenced by the depth of focus.
基金Project supported by the National Natural Science Foundation of China (Grants Nos.60736042,60578035 and 50703039)Science and Technology Cooperation Project between Chinese Academy of Sciences and Jilin Province (Grant No.2008SYHZ0005)
文摘This paper proposes a new Zernike modal gray map reconstruction algorithm used in the nematic liquid crystal adaptive optics system. Firstly, the new modal algorithm is described. Secondly, a single loop correction experiment was conducted, and it showed that the modal method has a higher precision in gray map reconstruction than the widely used slope method. Finally, the contrast close-loop correction experiment was conducted to correct static aberration in the laboratory. The experimental results showed that the average peak to valley （PV） and root mean square （RMS） of the wavefront corrected by mode method were reduced from 2.501A （λ= 633 nm） and 0.610A to 0.0334λ and 0.00845A, respectively. The corrected PV and RMS were much smaller than those of 0.173A and 0.048A by slope method. The Strehl ratio and modulation transfer function of the system corrected by mode method were much closer to diffraction limit than with slope method. These results indicate that the mode method can take good advantage of the large number of pixels of the liquid crystal corrector to realize high correction precision.
