The polymorphisms(Pvu Ⅱand Hind Ⅲ) on the lipoprotein lipase(LPL) gene locus was investigated in a sample of 100 patients surviving previous myocardial infarction and 100 age matched healthy individuals selected fro...The polymorphisms(Pvu Ⅱand Hind Ⅲ) on the lipoprotein lipase(LPL) gene locus was investigated in a sample of 100 patients surviving previous myocardial infarction and 100 age matched healthy individuals selected from Han Chinese of Beijing area.In patient group a strong association was found between H+allele of Hind Ⅲ polymorphism and raised TG levels(P<0.01).In control group P-P-genotype was observed to be associated with higher TG levels compared with P+P genotype of Pvu Ⅱ polymorphism(P<0.05).Combination of H+H+ genotype with P-P-genotype showed the highest TG levels among all nine kinds of genotype combinations in patient group(P<0.01).However,comparison of distribution of alleles and genotypes of these polymorphisms between patient group and control group demonstrated no significant difference. Our data suggest that the polymorphisms at the LPL gene,as the linkage markers with an aetiologic mutation at or around LPL gene,may constitute one of the genetic determinants for the population variation in plasma TG levels,as well as for the common dyslipidemia in Chinese population.展开更多
Objective： To explore the roles of persistent systemic inflammation in atherosclerosis and the effects of saponins of Panax Notoginseng （PNS） on this process in rabbits. Methods： Thirty rabbits were divided random...Objective： To explore the roles of persistent systemic inflammation in atherosclerosis and the effects of saponins of Panax Notoginseng （PNS） on this process in rabbits. Methods： Thirty rabbits were divided randomly and equally into 6 groups, i.e., control, high-fat diet, inflammation, aspirin, PNS and simple-inflammation group. All the animals except that in control group and simple-inflammation group were fed with high-fat diet for 8 weeks. Based on that, rabbits in inflammation, aspirin and PNS groups were treated with zymosan injection （10 mg/kg, i.p.）. Normal saline was given to rabbits in control group. Besides zymosan injection, animals in aspirin and PNS group were administrated with aspirin （12 mg/kg, i.g.） and PNS （120 mg/kg, i.g.） respectively. The animals in simple-inflammation group were treated with zymosan injection （10mg/kg, i.p.） and fed with normal diet. The atherosclerosis lesion in aortas was observed by Sudan IV staining. Serum total cholesterol, triglyceride （TG）, TNF-α and activity of post-heparin lipoprotein lipase （LPL） were measured at the end of the 4th and 8th week after an overnight fast. Results： Compared with high-fat diet group, the area of atherosclerosis lesion, serum TG and TNF-α were markedly increased in rabbits of inflammation group, and the activity of LPL was decreased remarkably. Serum TNF-α level was negatively correlated with the activity of post-heparin LPL （r=0.708, P〈0.01）. The area of atherosclerosis, serum TG and TNF-α were decreased in aspirin and PNS group compared with that in inflammation group, and the activity of LPL was increased remarkably. Compared with control group, serum TG and TNF-α were markedly increased in simple-inflammation group, while LPL activity was decreased. Atherosclerotic lesion did not occur in simple-inflammation group. Conclusion： Persistent systemic inflammation could accelerate the formation of atherosclerosis lesion in aortas, which partly depend on the decreasing of the activity of post-heparin lipoprotein lipase. PNS could improve the changes caused by inflammation.展开更多
文摘The polymorphisms(Pvu Ⅱand Hind Ⅲ) on the lipoprotein lipase(LPL) gene locus was investigated in a sample of 100 patients surviving previous myocardial infarction and 100 age matched healthy individuals selected from Han Chinese of Beijing area.In patient group a strong association was found between H+allele of Hind Ⅲ polymorphism and raised TG levels(P<0.01).In control group P-P-genotype was observed to be associated with higher TG levels compared with P+P genotype of Pvu Ⅱ polymorphism(P<0.05).Combination of H+H+ genotype with P-P-genotype showed the highest TG levels among all nine kinds of genotype combinations in patient group(P<0.01).However,comparison of distribution of alleles and genotypes of these polymorphisms between patient group and control group demonstrated no significant difference. Our data suggest that the polymorphisms at the LPL gene,as the linkage markers with an aetiologic mutation at or around LPL gene,may constitute one of the genetic determinants for the population variation in plasma TG levels,as well as for the common dyslipidemia in Chinese population.
基金Supported by the National Natural Science Foundation of China(30470465 and 30371768).
文摘Objective： To explore the roles of persistent systemic inflammation in atherosclerosis and the effects of saponins of Panax Notoginseng （PNS） on this process in rabbits. Methods： Thirty rabbits were divided randomly and equally into 6 groups, i.e., control, high-fat diet, inflammation, aspirin, PNS and simple-inflammation group. All the animals except that in control group and simple-inflammation group were fed with high-fat diet for 8 weeks. Based on that, rabbits in inflammation, aspirin and PNS groups were treated with zymosan injection （10 mg/kg, i.p.）. Normal saline was given to rabbits in control group. Besides zymosan injection, animals in aspirin and PNS group were administrated with aspirin （12 mg/kg, i.g.） and PNS （120 mg/kg, i.g.） respectively. The animals in simple-inflammation group were treated with zymosan injection （10mg/kg, i.p.） and fed with normal diet. The atherosclerosis lesion in aortas was observed by Sudan IV staining. Serum total cholesterol, triglyceride （TG）, TNF-α and activity of post-heparin lipoprotein lipase （LPL） were measured at the end of the 4th and 8th week after an overnight fast. Results： Compared with high-fat diet group, the area of atherosclerosis lesion, serum TG and TNF-α were markedly increased in rabbits of inflammation group, and the activity of LPL was decreased remarkably. Serum TNF-α level was negatively correlated with the activity of post-heparin LPL （r=0.708, P〈0.01）. The area of atherosclerosis, serum TG and TNF-α were decreased in aspirin and PNS group compared with that in inflammation group, and the activity of LPL was increased remarkably. Compared with control group, serum TG and TNF-α were markedly increased in simple-inflammation group, while LPL activity was decreased. Atherosclerotic lesion did not occur in simple-inflammation group. Conclusion： Persistent systemic inflammation could accelerate the formation of atherosclerosis lesion in aortas, which partly depend on the decreasing of the activity of post-heparin lipoprotein lipase. PNS could improve the changes caused by inflammation.
