The flower meristem identity gene LEAFY is a developmental switch,sufficient for flower initiation in diverse plants. Ginkgo biloba is a kind of gymnosperm,and a dioecious tree species.The nucleotide DNA sequence was ...The flower meristem identity gene LEAFY is a developmental switch,sufficient for flower initiation in diverse plants. Ginkgo biloba is a kind of gymnosperm,and a dioecious tree species.The nucleotide DNA sequence was cloned from the genomic DNA of the tender leaves collected from a male tree in Ginkgo biloba L.cv.Dafushou.The analysis of DNA sequence structure indicated that the gene was composed of 3450 bp,and inferred the sequence to LEAFY gene of the male Ginkgo .Comparing the gene with LEAFY gene of the female Ginkgo reported in the relative references,the rate of homology was 99%,only lacking 3 nucleotides.The research work laid a sound foundation for studing the regulation and controlling flowering mechanism in Ginkgo biloba L.at the molecuar level in the future.展开更多
Clausena lansium, Citrus sinensis and Poncirus trifoliata belong to the different genus of Rutaceae. On the basis of LEAFY (LFY in brief) gene sequences of C. sinensis and P. trifoliata, a pair of 21 bp primers was de...Clausena lansium, Citrus sinensis and Poncirus trifoliata belong to the different genus of Rutaceae. On the basis of LEAFY (LFY in brief) gene sequences of C. sinensis and P. trifoliata, a pair of 21 bp primers was designed and used to amplify about a 2 300 bp DNA fragment from C. lansium by PCR. The DNA fragment was cloned into pMD18-T vector and then sequenced. The sequence analysis showed that the genomic DNA clone with 2 346 bp contained a complete coding sequence of LFY homologous gene (ClLFY) which 3 exons were composed of a completed open reading frame and encoded 397 amino acids. The identities of nucleotide sequences of LFY genes between C. lansium with C. sinensis and P. trifoliata were more than 92%, and their identities of amino acid sequences were 95%.The result also indicated that C. lansium LFY gene was one amino acid less than the LFY homologous genes from C. sinensis and P. trifoliata.The research laid a sound foundation for studying the regulation and control in flowering mechanism in C. lansium at the molecular level in the future.展开更多
The list of underutilized green leafy vegetables is enormous: ranging from commonly consumed and underutilized vegetables. There are data on commonly consumed vegetables, but information is sparse on nutritional, chem...The list of underutilized green leafy vegetables is enormous: ranging from commonly consumed and underutilized vegetables. There are data on commonly consumed vegetables, but information is sparse on nutritional, chemical and anti-nutritional properties of less explored vegetables. This work aimed to evaluate some attributes of less explored or underutilized leafy vegetables. Some nutritional and anti-nutritional contents of 10 under-utilized leafy vegetables(Amaranthus spinosus Linnaeus, Basella alba Linnaeus, Corchorous tridens L., Cyrtosperma senegalense(Schott), Erigeron floribundus Sch, Hoslundia opposita Vahl, Lagenaria siceraria(Molina) Standl, Ocimum basilicum L., Solanum aethiopicum L. and Talinum portulacifolium(Forssk.) Asch. ex Schweinf) were investigated. Cyrtosperma senegalense had the highest protein value(5.93%). Hoslundia opposita had the highest fat content(0.54%). Amaranthus spinosus was high in β-carotene content(346.3 mg · 100 g-1); Cyrtosperma senegalense had the highest moisture content. A. spinosus had the highest ascorbic acid content(108.1 mg · 100 g-1). The highest tannin level(0.0057%) was found in C. senegalense and the lowest(0.0003%) was found in Solanum aethiopicum. Saponin was the highest in C. tridens(0.0052%) and the lowest in T. portulacifolium(0.00014%). C. senegalense and S. aethiopicum had their highest and the lowest phytate values, respectively. Although anti-nutrients could interfere with nutrient utilization, their values in these vegetables were not at toxic levels.展开更多
文摘The flower meristem identity gene LEAFY is a developmental switch,sufficient for flower initiation in diverse plants. Ginkgo biloba is a kind of gymnosperm,and a dioecious tree species.The nucleotide DNA sequence was cloned from the genomic DNA of the tender leaves collected from a male tree in Ginkgo biloba L.cv.Dafushou.The analysis of DNA sequence structure indicated that the gene was composed of 3450 bp,and inferred the sequence to LEAFY gene of the male Ginkgo .Comparing the gene with LEAFY gene of the female Ginkgo reported in the relative references,the rate of homology was 99%,only lacking 3 nucleotides.The research work laid a sound foundation for studing the regulation and controlling flowering mechanism in Ginkgo biloba L.at the molecuar level in the future.
文摘Clausena lansium, Citrus sinensis and Poncirus trifoliata belong to the different genus of Rutaceae. On the basis of LEAFY (LFY in brief) gene sequences of C. sinensis and P. trifoliata, a pair of 21 bp primers was designed and used to amplify about a 2 300 bp DNA fragment from C. lansium by PCR. The DNA fragment was cloned into pMD18-T vector and then sequenced. The sequence analysis showed that the genomic DNA clone with 2 346 bp contained a complete coding sequence of LFY homologous gene (ClLFY) which 3 exons were composed of a completed open reading frame and encoded 397 amino acids. The identities of nucleotide sequences of LFY genes between C. lansium with C. sinensis and P. trifoliata were more than 92%, and their identities of amino acid sequences were 95%.The result also indicated that C. lansium LFY gene was one amino acid less than the LFY homologous genes from C. sinensis and P. trifoliata.The research laid a sound foundation for studying the regulation and control in flowering mechanism in C. lansium at the molecular level in the future.
文摘The list of underutilized green leafy vegetables is enormous: ranging from commonly consumed and underutilized vegetables. There are data on commonly consumed vegetables, but information is sparse on nutritional, chemical and anti-nutritional properties of less explored vegetables. This work aimed to evaluate some attributes of less explored or underutilized leafy vegetables. Some nutritional and anti-nutritional contents of 10 under-utilized leafy vegetables(Amaranthus spinosus Linnaeus, Basella alba Linnaeus, Corchorous tridens L., Cyrtosperma senegalense(Schott), Erigeron floribundus Sch, Hoslundia opposita Vahl, Lagenaria siceraria(Molina) Standl, Ocimum basilicum L., Solanum aethiopicum L. and Talinum portulacifolium(Forssk.) Asch. ex Schweinf) were investigated. Cyrtosperma senegalense had the highest protein value(5.93%). Hoslundia opposita had the highest fat content(0.54%). Amaranthus spinosus was high in β-carotene content(346.3 mg · 100 g-1); Cyrtosperma senegalense had the highest moisture content. A. spinosus had the highest ascorbic acid content(108.1 mg · 100 g-1). The highest tannin level(0.0057%) was found in C. senegalense and the lowest(0.0003%) was found in Solanum aethiopicum. Saponin was the highest in C. tridens(0.0052%) and the lowest in T. portulacifolium(0.00014%). C. senegalense and S. aethiopicum had their highest and the lowest phytate values, respectively. Although anti-nutrients could interfere with nutrient utilization, their values in these vegetables were not at toxic levels.
