According to the sequence of the bile salt hydrolase （BSH） gene of Bifidobacterium and the restriction enzyme cutting sites of expression vector pNZ8148, primers were designed and the bile salt hydrolase （BSH） gen...According to the sequence of the bile salt hydrolase （BSH） gene of Bifidobacterium and the restriction enzyme cutting sites of expression vector pNZ8148, primers were designed and the bile salt hydrolase （BSH） gene was gotten from Bacillus bifidus ATCC 29521 by PCR. BSH gene was inserted into lactic acid bacteria expression vector pNZ8148 to construct the recombinant pNZ8148-BSH. The recombinant pNZ8148-BSH was transferred into lactic acid bacteria NZ9000 with electrotransformation method. And the recombinant which could express BSH protein was obtained. It was identified by SDS-PAGE electrophoresis and activity verification. The result could provide a rationale reference for expressing BSH in lactic acid bacteria.展开更多
Eight cholesterol-lowering lactic acid bacteria(LAB 1 through 8)isolated from pickles and yogurt were evaluated for probiotic potential based on their tolerance to low pH and bile salt and antagonistic activity agains...Eight cholesterol-lowering lactic acid bacteria(LAB 1 through 8)isolated from pickles and yogurt were evaluated for probiotic potential based on their tolerance to low pH and bile salt and antagonistic activity against Staphylococcus aureusand Escherichia coli.Strain LAB 4 had excellent tolerance to low pH and bile salt and all 8 LAB strains had antagonisticactivity against Staphylococcus aureus and Escherichia coli.Moreover,strain LAB4 had synergic cholesterol-lowering effect with 0.0125%licorice root extract,0.025%Chinese wolfberry extract or 0.025%milkvetch root extract.展开更多
基金Supported by 863 Projects (2008AA10Z311)National Science and Technology Support Projects (2009BADB9B06)+1 种基金Started Post-doctoral Research Grant of Heilongjiang Province (LBH-Q07023)Harbin Technological Innovation of Special Funds (2007RFQXN020)
文摘According to the sequence of the bile salt hydrolase （BSH） gene of Bifidobacterium and the restriction enzyme cutting sites of expression vector pNZ8148, primers were designed and the bile salt hydrolase （BSH） gene was gotten from Bacillus bifidus ATCC 29521 by PCR. BSH gene was inserted into lactic acid bacteria expression vector pNZ8148 to construct the recombinant pNZ8148-BSH. The recombinant pNZ8148-BSH was transferred into lactic acid bacteria NZ9000 with electrotransformation method. And the recombinant which could express BSH protein was obtained. It was identified by SDS-PAGE electrophoresis and activity verification. The result could provide a rationale reference for expressing BSH in lactic acid bacteria.
文摘Eight cholesterol-lowering lactic acid bacteria(LAB 1 through 8)isolated from pickles and yogurt were evaluated for probiotic potential based on their tolerance to low pH and bile salt and antagonistic activity against Staphylococcus aureusand Escherichia coli.Strain LAB 4 had excellent tolerance to low pH and bile salt and all 8 LAB strains had antagonisticactivity against Staphylococcus aureus and Escherichia coli.Moreover,strain LAB4 had synergic cholesterol-lowering effect with 0.0125%licorice root extract,0.025%Chinese wolfberry extract or 0.025%milkvetch root extract.
