Several clinical studies have shown a large number of mental symptoms by immunomodulatory treatment with interferon (IFN). The most frequently described symptoms are depression, suicidal behaviour, manic symptoms, anx...Several clinical studies have shown a large number of mental symptoms by immunomodulatory treatment with interferon (IFN). The most frequently described symptoms are depression, suicidal behaviour, manic symptoms, anxiety, psychosis and delirium, associated with other non-specific symptoms such as fatigue, irritability, psychomotor retardation, decreased libido, insomnia, difficulty in concentration and attention. Having a history of mental disorder contraindicates the use of IFN-alpha. These adverse effects that affect the mental state appear usually at the beginning of the treatment (most after 3 weeks of treatment). The incidence of psychotic episodes is low and the episodes usually remit when treatment is interrupted;only some cases require antipsychotic treatment. We present the case of a patient affected with hepatitis C who began to present self-referential delirious symptoms after receiving the treatment with IFN and who was successfully treated with paliperidone. This patient could be classified within the group of high-risk psychiatric patients given the family history of schizophrenia and his personal history of illegal drug consumption. The pharmacological actions of paliperidone are similar to other high potency atypical antipsychotics.丁he receptorbinding profile of paliperidone most closely resembles that of risperidone and ziprasidone. Paliperidone differs from risperidone and most other antipsychotics by its relatively low extent of enzymatic hepatic metabolism. To the best of our knowledge, this is the first case described that was successfully treated with paliperidone.展开更多
Six patients with chronic hepatitis B were investigated for hepatitis B virus (HBV) DNA both in serum and in peripheral blood mononuclear cells (PBMC) by polymerase chain reaction (PCR) before ,during and after interf...Six patients with chronic hepatitis B were investigated for hepatitis B virus (HBV) DNA both in serum and in peripheral blood mononuclear cells (PBMC) by polymerase chain reaction (PCR) before ,during and after interferon alpha (IFN-a)treatment.All patients responded to IFN therapy with remission of serum alanine aminotransferase (ALT).HBV DNA disappeared in serum of 6 and in PBMCs of 5 patients during the treatment with IFN-a. The average elimination period was 5 weeks (range 3-10 weeks) in serum and 15 weeks (range 12-20weeks) in PBMCs. Relapse of serum ALT was seen in one patient with HBV DNA in PBMCs persistently positive during and after treatment with IFN-a. The results showed that clearance of HBV DNA was more difficult in PBMCs than that in serum,and that the persistent appearance of HBV DNA in PBMCs during and after treatment with IFN-a may imply the failure of IFN therapy.展开更多
Objective: To investigate the effect of tazarotene on the expression of HLA-DR induced by IFN-γ. Methods: (1) Keratinocytes from normal human skin were cultured in vitro;(2) Tazarotene, IFN-γ and the combination of ...Objective: To investigate the effect of tazarotene on the expression of HLA-DR induced by IFN-γ. Methods: (1) Keratinocytes from normal human skin were cultured in vitro;(2) Tazarotene, IFN-γ and the combination of the two compounds were incubated with the keratinocytes in medium, respectively. The expression of HLA-DR in keratinocytes was determined using immunocytochemistry techniques at 24h after incubation. Results: (1) There was rare expression of HLA-DR in normal human keratinocytes; (2) 10 -6mol/L tazarotene failed to induce the expression of HLA-DR in keratinocytes at 24h after incubation; (3) 500 U/ml IFN-γ obviously induced the HLA-DR expression in keratinocytes at 24h after treatment; (4) After 24h, 10 -7-10 -5 mol/L tazarotene had a significantly enhancing effect on the expression of HLA-DR induced by IFN-γ (P<0.005). Conclusion: Tazarotene up-regulates the expression of HLA-DR in keratinocytes cultured in vitro when combined with IFN-γ . Therefore, the reduction of HLA-DR positive keratinocytes in psoriatic lesions may be attributed to not direct interaction of tazarotene in combination with IFN-γ but other pathways.展开更多
Objective To investigate the antiviral activity of recombinant interferonα-2b suppository(IFNα-2b)in vivo and in vitro.Methods The cytopathic-effect inhibition assay was applied in this study to investigate the anti...Objective To investigate the antiviral activity of recombinant interferonα-2b suppository(IFNα-2b)in vivo and in vitro.Methods The cytopathic-effect inhibition assay was applied in this study to investigate the antiviral activity of this drug as well as yingtelong and axiluowei as positive control.The guinea pig model of vaginitis and skin infection caused by HSV-2 infection were established,treated with IFNα-2b suppository at dosages of 60000、180000、540000 IU,using IFNα-2b injection 180000 IU·kg-1 as controls.Score the pathological changes of appearance and skin,the virus activities of vaginal secretion and tissue sections of viginae were assayed after treatment.Results The TD50 of IFN α-2b and yingtelong for Vero cells was(>100)μg·mL-1 and(>100000)IU·mL-1,respectively.The IC50 of IFN α-2b and yingtelong and axiluowei for Herpes virus type 1 was(0.29±0.08)μg·mL-1 and(185.0±28.8)IU·mL-1 and(0.19±0.03)μg·mL-1,respectively.The mean scores for vaginal and skin lesion of the treated groups were lower than those of untreated group.Among these concentrations,the IFNα-2b suppository of 540000 IU·kg-1 group.Showed highest anti-viral activity.The virus activity in vaginal secretion of treated group was lower than that of untreated group too(P<0.01 or P<0.05).Tissue sections of viginae after treatment with IFNα-2b suppository showed significantly therapeutical effects on the degrees of vaginal lesion.At the same dosage,The anti-HSV activity of IFNα-2b suppository was also compared with IFNα-2b injection,the results showed that the activity of suppository of 540000 IU·kg-1 group was similar to that of the injection.Conclusions The IFNα-2b suppository has anti-viruses function both in vivo and in vitro.展开更多
The effects of IFN on the colony-forming ability,phagocytosis andmorphology of cells of murine CFU-GM were reported.The results showed thatIFN could inhibit the proliferation of CFU-GM,but 100IU IFN might promotethe p...The effects of IFN on the colony-forming ability,phagocytosis andmorphology of cells of murine CFU-GM were reported.The results showed thatIFN could inhibit the proliferation of CFU-GM,but 100IU IFN might promotethe phagocytosis of macrophagocytic cells.The significance of IFN in the regula-tion of hemopoiesis and the pathogenesis of aplastic anemia was discussed.展开更多
Objective:To investigate the reversing effects of interferon a (IFN-a) on drug resistance of bone marrow cells in leukemia and its possible mechanism. Methods: Before and after the bone marrow cells extracted from50 c...Objective:To investigate the reversing effects of interferon a (IFN-a) on drug resistance of bone marrow cells in leukemia and its possible mechanism. Methods: Before and after the bone marrow cells extracted from50 cases of acute leukemia and 2 of chronic leukemia (BP) were incubated in the media with the addition of IFN-α,MDR1 mRNA expression and concentration of daunorubicin (DNR) in the cells were determined respectively withSAG-ISH and respectrofluorimetry. Results: Twenty-four (64. 2% ) our of all the 52 cases presented MDR1 mRNA expression. There was significant difference in the concentration of DNR between MDR1-positive and MDR1-negative cases before and after incubation. In all the 52 cases, no significant difference was found between the concentration of DNR before the incubation and that after the incubation. Conclusion: IFN-a can increase the concentration of DNR in the bone marrow cells in leukemia. No occurrence of MDR reversal is on the level of MDRl buton other levels.展开更多
Human interferon alpha-8(IFN-α8) is an important cytokine with multiple biological functions.A genetically engineered strain, E. coli XL1-Blue/pBm, was constructed by DNA recombination technology and characterized by...Human interferon alpha-8(IFN-α8) is an important cytokine with multiple biological functions.A genetically engineered strain, E. coli XL1-Blue/pBm, was constructed by DNA recombination technology and characterized by restriction analysis, DNA sequencing.展开更多
Objective：To investigate the effects and mechanisms of interferon in combination with alltrans retinoic acid （ATRA） on proliferation and differentiation of ATRA-resistent APL cell. Methods ：After MR2 cells （ATRA-...Objective：To investigate the effects and mechanisms of interferon in combination with alltrans retinoic acid （ATRA） on proliferation and differentiation of ATRA-resistent APL cell. Methods ：After MR2 cells （ATRA-resistance cell line） were treated with IFN-α, IFN-γ and ATRA alone or IFN-α and IFN-γ in combination with ATRA respectively. The cell proliferation was tested by MTT test and the cell differentiation was tested through light microscope by NBT test and flow cytometry （FCM）. The expres sion of promyelocytic leukemia （PML） protein was observed by indirect immune fluorescent method. Results： Both IFN-α and IFN-γ could inhibit the proliferation and induce the differentiation of MR2 cells to some extent. The effects were more obvious after both interferons in combination with ATRA respectively （P〈0.05）. Moreover, the maturation of MR2 cells induced by IFN-γ＋ATRA group was more higher than that by IFN-α＋ATRA group （P〈0. 05）. Both interferons could induce the expressions of PML protein. Conclusion：Both interferons can inhibit MR2 cells proliferation, which may be related to the expression of PML protein induced by both interferons. The inducing differentiation effects of IFN-γ＋ATRA group on MR2 cells are more powerful than those of IFN-aq-ATRA group, which may be related to the different signal transduction pathway of both interferons.展开更多
Objective: To study the effect of intracerebroventricular administration of interferon (IFN-α) on the splenic sympathetic nerve activity. Methods: IFN-α (3×104 U/rat ) was microinjected into the third cerebrove...Objective: To study the effect of intracerebroventricular administration of interferon (IFN-α) on the splenic sympathetic nerve activity. Methods: IFN-α (3×104 U/rat ) was microinjected into the third cerebroven-tricle of urethane and α-chloralose anesthetized rats. Electrical activity of the splenic nerve. body temperature and blood pressure were determined simultaneously. Results: Intracerebroventricular administration of IFN-α caused amarked and long term (>120 min) increase in the splenic nerve activity, which could be reversed by naloxonetreatment. No changes in blood pressure and body temperature. which could alter the sympathetic nerve actlvity by reflex. were observed during IFN-α and naloxone treatment. Naloxone intravenous injection alone had no effecton the splenic nerve activity. Conclusion: IFN-α in the brain can activate the splenic sympathetic nerve, and thisaction is, in some way, mediated by the opioid receptor.展开更多
Establishing the hepatoma cell-specific expression of human interferon gene mediated by retroviral vectors. Methods: Human interferon-β complementary DNA (IFN-β cDNA) was inserted into polylinker site of pMNSM retro...Establishing the hepatoma cell-specific expression of human interferon gene mediated by retroviral vectors. Methods: Human interferon-β complementary DNA (IFN-β cDNA) was inserted into polylinker site of pMNSM retroviral vector to construct recombinant retroviral vector pMNSIFNB, where the transcription of IFN-β gene was driven by SV40 early region promoter, and MNAIFNB, where the transcription of IFN-β gene was driven by SV40 early region promoter regulated by α-fetoprotein enhancer. The retroviral constructs were respectively introduced into PA317 amphotropic packaging cells by means of lipofectamine mediated gene transfer procedure. The plasmids transfection efficiency was among (4-25)ｘ103 colonies/μg DNA/106 PA317 cells. The retrovirus infection efficiency was among (4. 5-500)ｘ104 Colony Forming Units (CFU)/ml. The recombinant retroviruses were used to infect human hepatoma cells, renal cell carcinoma cells and melanoma cell lines in the presence of 4 μg/ml polybrene. Results: Dot hybridization of total RNA from the neomycin resistant colonies and interferon expression assay indicated that human α-fetoprotein enhancer induced efficient and specific transcription and expression of IFN-β gene driven by the promoter of different origin in human hepatoma cells by which α-fetoprotein was highly produced. Conclusion: Cis-active element of α-fetoprotein gene can drive IFN-β gene specifically expressed in human hepatoma cells, which presents some valuable materials for the hepatoma-specific immune gene therapy.展开更多
目的基于网状Meta分析系统评价重组干扰素α-2b治疗小儿疱疹性咽峡炎的疗效及安全性。方法计算机检索中国生物医学文献(CBM)、中国知网、万方、维普、读秀、Ovid、Cochrane Library、Embase、PubMed、Web of Science等数据库,纳入重组...目的基于网状Meta分析系统评价重组干扰素α-2b治疗小儿疱疹性咽峡炎的疗效及安全性。方法计算机检索中国生物医学文献(CBM)、中国知网、万方、维普、读秀、Ovid、Cochrane Library、Embase、PubMed、Web of Science等数据库,纳入重组干扰素α-2b治疗疱疹性咽峡炎患儿(年龄≤7岁)的随机对照试验,检索时限为建库至2024年2月27日。2名独立的研究者遵循纳入与排除标准,采用改良后的Jadad量表和Cochrane偏倚风险评估工具,对文献进行筛选、资料提取和质量评价。提取临床总有效率、退热时间、疱疹消失时间、恢复进食时间、住院时间及不良反应发生率等结局指标,通过Stata16.0软件对筛选出的文献进行Meta网状分析。结果最终纳入24篇文献,共计3078例疱疹性咽峡炎患儿,对照组干预措施包括利巴韦林、常规治疗、喜炎平、开喉剑及热毒宁等。网状Meta分析结果显示,与对照组不同干预措施相比,试验组采用重组干扰素α-2b可提高临床总有效率,缩短退热时间、疱疹消失时间、恢复进食时间、住院时间(P均<0.05),且不会增加不良反应发生率。结论对比其他治疗方案,使用重组干扰素α-2b治疗小儿疱疹性咽峡炎疗效显著,安全性较高。展开更多
文摘Several clinical studies have shown a large number of mental symptoms by immunomodulatory treatment with interferon (IFN). The most frequently described symptoms are depression, suicidal behaviour, manic symptoms, anxiety, psychosis and delirium, associated with other non-specific symptoms such as fatigue, irritability, psychomotor retardation, decreased libido, insomnia, difficulty in concentration and attention. Having a history of mental disorder contraindicates the use of IFN-alpha. These adverse effects that affect the mental state appear usually at the beginning of the treatment (most after 3 weeks of treatment). The incidence of psychotic episodes is low and the episodes usually remit when treatment is interrupted;only some cases require antipsychotic treatment. We present the case of a patient affected with hepatitis C who began to present self-referential delirious symptoms after receiving the treatment with IFN and who was successfully treated with paliperidone. This patient could be classified within the group of high-risk psychiatric patients given the family history of schizophrenia and his personal history of illegal drug consumption. The pharmacological actions of paliperidone are similar to other high potency atypical antipsychotics.丁he receptorbinding profile of paliperidone most closely resembles that of risperidone and ziprasidone. Paliperidone differs from risperidone and most other antipsychotics by its relatively low extent of enzymatic hepatic metabolism. To the best of our knowledge, this is the first case described that was successfully treated with paliperidone.
文摘Six patients with chronic hepatitis B were investigated for hepatitis B virus (HBV) DNA both in serum and in peripheral blood mononuclear cells (PBMC) by polymerase chain reaction (PCR) before ,during and after interferon alpha (IFN-a)treatment.All patients responded to IFN therapy with remission of serum alanine aminotransferase (ALT).HBV DNA disappeared in serum of 6 and in PBMCs of 5 patients during the treatment with IFN-a. The average elimination period was 5 weeks (range 3-10 weeks) in serum and 15 weeks (range 12-20weeks) in PBMCs. Relapse of serum ALT was seen in one patient with HBV DNA in PBMCs persistently positive during and after treatment with IFN-a. The results showed that clearance of HBV DNA was more difficult in PBMCs than that in serum,and that the persistent appearance of HBV DNA in PBMCs during and after treatment with IFN-a may imply the failure of IFN therapy.
文摘Objective: To investigate the effect of tazarotene on the expression of HLA-DR induced by IFN-γ. Methods: (1) Keratinocytes from normal human skin were cultured in vitro;(2) Tazarotene, IFN-γ and the combination of the two compounds were incubated with the keratinocytes in medium, respectively. The expression of HLA-DR in keratinocytes was determined using immunocytochemistry techniques at 24h after incubation. Results: (1) There was rare expression of HLA-DR in normal human keratinocytes; (2) 10 -6mol/L tazarotene failed to induce the expression of HLA-DR in keratinocytes at 24h after incubation; (3) 500 U/ml IFN-γ obviously induced the HLA-DR expression in keratinocytes at 24h after treatment; (4) After 24h, 10 -7-10 -5 mol/L tazarotene had a significantly enhancing effect on the expression of HLA-DR induced by IFN-γ (P<0.005). Conclusion: Tazarotene up-regulates the expression of HLA-DR in keratinocytes cultured in vitro when combined with IFN-γ . Therefore, the reduction of HLA-DR positive keratinocytes in psoriatic lesions may be attributed to not direct interaction of tazarotene in combination with IFN-γ but other pathways.
文摘Objective To investigate the antiviral activity of recombinant interferonα-2b suppository(IFNα-2b)in vivo and in vitro.Methods The cytopathic-effect inhibition assay was applied in this study to investigate the antiviral activity of this drug as well as yingtelong and axiluowei as positive control.The guinea pig model of vaginitis and skin infection caused by HSV-2 infection were established,treated with IFNα-2b suppository at dosages of 60000、180000、540000 IU,using IFNα-2b injection 180000 IU·kg-1 as controls.Score the pathological changes of appearance and skin,the virus activities of vaginal secretion and tissue sections of viginae were assayed after treatment.Results The TD50 of IFN α-2b and yingtelong for Vero cells was(>100)μg·mL-1 and(>100000)IU·mL-1,respectively.The IC50 of IFN α-2b and yingtelong and axiluowei for Herpes virus type 1 was(0.29±0.08)μg·mL-1 and(185.0±28.8)IU·mL-1 and(0.19±0.03)μg·mL-1,respectively.The mean scores for vaginal and skin lesion of the treated groups were lower than those of untreated group.Among these concentrations,the IFNα-2b suppository of 540000 IU·kg-1 group.Showed highest anti-viral activity.The virus activity in vaginal secretion of treated group was lower than that of untreated group too(P<0.01 or P<0.05).Tissue sections of viginae after treatment with IFNα-2b suppository showed significantly therapeutical effects on the degrees of vaginal lesion.At the same dosage,The anti-HSV activity of IFNα-2b suppository was also compared with IFNα-2b injection,the results showed that the activity of suppository of 540000 IU·kg-1 group was similar to that of the injection.Conclusions The IFNα-2b suppository has anti-viruses function both in vivo and in vitro.
文摘The effects of IFN on the colony-forming ability,phagocytosis andmorphology of cells of murine CFU-GM were reported.The results showed thatIFN could inhibit the proliferation of CFU-GM,but 100IU IFN might promotethe phagocytosis of macrophagocytic cells.The significance of IFN in the regula-tion of hemopoiesis and the pathogenesis of aplastic anemia was discussed.
文摘Objective:To investigate the reversing effects of interferon a (IFN-a) on drug resistance of bone marrow cells in leukemia and its possible mechanism. Methods: Before and after the bone marrow cells extracted from50 cases of acute leukemia and 2 of chronic leukemia (BP) were incubated in the media with the addition of IFN-α,MDR1 mRNA expression and concentration of daunorubicin (DNR) in the cells were determined respectively withSAG-ISH and respectrofluorimetry. Results: Twenty-four (64. 2% ) our of all the 52 cases presented MDR1 mRNA expression. There was significant difference in the concentration of DNR between MDR1-positive and MDR1-negative cases before and after incubation. In all the 52 cases, no significant difference was found between the concentration of DNR before the incubation and that after the incubation. Conclusion: IFN-a can increase the concentration of DNR in the bone marrow cells in leukemia. No occurrence of MDR reversal is on the level of MDRl buton other levels.
文摘Human interferon alpha-8(IFN-α8) is an important cytokine with multiple biological functions.A genetically engineered strain, E. coli XL1-Blue/pBm, was constructed by DNA recombination technology and characterized by restriction analysis, DNA sequencing.
文摘Objective：To investigate the effects and mechanisms of interferon in combination with alltrans retinoic acid （ATRA） on proliferation and differentiation of ATRA-resistent APL cell. Methods ：After MR2 cells （ATRA-resistance cell line） were treated with IFN-α, IFN-γ and ATRA alone or IFN-α and IFN-γ in combination with ATRA respectively. The cell proliferation was tested by MTT test and the cell differentiation was tested through light microscope by NBT test and flow cytometry （FCM）. The expres sion of promyelocytic leukemia （PML） protein was observed by indirect immune fluorescent method. Results： Both IFN-α and IFN-γ could inhibit the proliferation and induce the differentiation of MR2 cells to some extent. The effects were more obvious after both interferons in combination with ATRA respectively （P〈0.05）. Moreover, the maturation of MR2 cells induced by IFN-γ＋ATRA group was more higher than that by IFN-α＋ATRA group （P〈0. 05）. Both interferons could induce the expressions of PML protein. Conclusion：Both interferons can inhibit MR2 cells proliferation, which may be related to the expression of PML protein induced by both interferons. The inducing differentiation effects of IFN-γ＋ATRA group on MR2 cells are more powerful than those of IFN-aq-ATRA group, which may be related to the different signal transduction pathway of both interferons.
文摘Objective: To study the effect of intracerebroventricular administration of interferon (IFN-α) on the splenic sympathetic nerve activity. Methods: IFN-α (3×104 U/rat ) was microinjected into the third cerebroven-tricle of urethane and α-chloralose anesthetized rats. Electrical activity of the splenic nerve. body temperature and blood pressure were determined simultaneously. Results: Intracerebroventricular administration of IFN-α caused amarked and long term (>120 min) increase in the splenic nerve activity, which could be reversed by naloxonetreatment. No changes in blood pressure and body temperature. which could alter the sympathetic nerve actlvity by reflex. were observed during IFN-α and naloxone treatment. Naloxone intravenous injection alone had no effecton the splenic nerve activity. Conclusion: IFN-α in the brain can activate the splenic sympathetic nerve, and thisaction is, in some way, mediated by the opioid receptor.
文摘Establishing the hepatoma cell-specific expression of human interferon gene mediated by retroviral vectors. Methods: Human interferon-β complementary DNA (IFN-β cDNA) was inserted into polylinker site of pMNSM retroviral vector to construct recombinant retroviral vector pMNSIFNB, where the transcription of IFN-β gene was driven by SV40 early region promoter, and MNAIFNB, where the transcription of IFN-β gene was driven by SV40 early region promoter regulated by α-fetoprotein enhancer. The retroviral constructs were respectively introduced into PA317 amphotropic packaging cells by means of lipofectamine mediated gene transfer procedure. The plasmids transfection efficiency was among (4-25)ｘ103 colonies/μg DNA/106 PA317 cells. The retrovirus infection efficiency was among (4. 5-500)ｘ104 Colony Forming Units (CFU)/ml. The recombinant retroviruses were used to infect human hepatoma cells, renal cell carcinoma cells and melanoma cell lines in the presence of 4 μg/ml polybrene. Results: Dot hybridization of total RNA from the neomycin resistant colonies and interferon expression assay indicated that human α-fetoprotein enhancer induced efficient and specific transcription and expression of IFN-β gene driven by the promoter of different origin in human hepatoma cells by which α-fetoprotein was highly produced. Conclusion: Cis-active element of α-fetoprotein gene can drive IFN-β gene specifically expressed in human hepatoma cells, which presents some valuable materials for the hepatoma-specific immune gene therapy.
文摘目的基于网状Meta分析系统评价重组干扰素α-2b治疗小儿疱疹性咽峡炎的疗效及安全性。方法计算机检索中国生物医学文献(CBM)、中国知网、万方、维普、读秀、Ovid、Cochrane Library、Embase、PubMed、Web of Science等数据库,纳入重组干扰素α-2b治疗疱疹性咽峡炎患儿(年龄≤7岁)的随机对照试验,检索时限为建库至2024年2月27日。2名独立的研究者遵循纳入与排除标准,采用改良后的Jadad量表和Cochrane偏倚风险评估工具,对文献进行筛选、资料提取和质量评价。提取临床总有效率、退热时间、疱疹消失时间、恢复进食时间、住院时间及不良反应发生率等结局指标,通过Stata16.0软件对筛选出的文献进行Meta网状分析。结果最终纳入24篇文献,共计3078例疱疹性咽峡炎患儿,对照组干预措施包括利巴韦林、常规治疗、喜炎平、开喉剑及热毒宁等。网状Meta分析结果显示,与对照组不同干预措施相比,试验组采用重组干扰素α-2b可提高临床总有效率,缩短退热时间、疱疹消失时间、恢复进食时间、住院时间(P均<0.05),且不会增加不良反应发生率。结论对比其他治疗方案,使用重组干扰素α-2b治疗小儿疱疹性咽峡炎疗效显著,安全性较高。
