Ischemia is a significant factor affecting the repair of peripheral nerve injuries,while exosomes have been shown to promote angiogenesis.To further investigate the detailed processes and efficacy of exosome thera⁃py ...Ischemia is a significant factor affecting the repair of peripheral nerve injuries,while exosomes have been shown to promote angiogenesis.To further investigate the detailed processes and efficacy of exosome thera⁃py for ischemic peripheral nerve injuries,this study utilized glucose-modified near-infrared-II(NIR-II)quantum dots(QDs)to label adipose-derived stem cell exosomes(QDs-ADSC-Exos),enabling long-term in vivo NIR-II imaging of exosome treatment for ischemic peripheral nerve damage.Experimental results confirmed that QDs can be used for non-invasive in vitro labeling of exosomes,with QDs-ADSC-Exos exhibiting strong fluorescence signals in the NIR-II window and demonstrating favorable NIR-II imaging characteristics in vivo.Notably,QDsADSC-Exos showed accumulation at the site of nerve injury in cases of ischemic peripheral nerve damage.Func⁃tional neurological assessments indicated that QDs-ADSC-Exos effectively promoted neural regeneration.This study highlights the potential of exosomes in treating ischemic peripheral nerve injuries and elucidates the spatio⁃temporal characteristics of exosome therapy,providing objective evidence for the further optimization of exosomebased treatment protocols.展开更多
在法医学检案中,经常要对尸体上的损伤进行检验以确定生前伤或死后伤,并要鉴定损伤时间.损伤超过8小时者一般用常规组织学方法可以解决,但损伤后经过时间较短,单用组织学方法不能解决,如利用酶活性反应方法和利用在组织内细胞内进行特...在法医学检案中,经常要对尸体上的损伤进行检验以确定生前伤或死后伤,并要鉴定损伤时间.损伤超过8小时者一般用常规组织学方法可以解决,但损伤后经过时间较短,单用组织学方法不能解决,如利用酶活性反应方法和利用在组织内细胞内进行特异、灵敏、稳定的抗原抗体反应,可以较准确鉴定损伤时间.Perper J. A. Raek Allio、Tanaka、Fatteh等报导用组织化学方法鉴定损伤时间,祝家镇、刘世仓教授近年来也有报道,但用免疫组化学方法来鉴定损伤后早期时间,就作者所知,国内、外未见报道,而FN在创伤愈合过程中的作用国内外已有报道.展开更多
In order to explore the effect of electroacupuncture on rats with acute spinal injury,we established animal model of the spinal injury.Total proteins were extracted from spinal cord of normal rats,acute spinal injury ...In order to explore the effect of electroacupuncture on rats with acute spinal injury,we established animal model of the spinal injury.Total proteins were extracted from spinal cord of normal rats,acute spinal injury rats and acute spinal injury rat treated by electroacupuncture,and separated by 2-DE.10 differential expressed proteins were identified by the MALDI-TOF-TOF-MS with high mass accuracy,high mascot scores and high reliability.展开更多
基金Supported by the National Natural Science Foundation of China(82371373,W2412120)the Shanghai Natural Science Foundation(21ZR1436100).
文摘Ischemia is a significant factor affecting the repair of peripheral nerve injuries,while exosomes have been shown to promote angiogenesis.To further investigate the detailed processes and efficacy of exosome thera⁃py for ischemic peripheral nerve injuries,this study utilized glucose-modified near-infrared-II(NIR-II)quantum dots(QDs)to label adipose-derived stem cell exosomes(QDs-ADSC-Exos),enabling long-term in vivo NIR-II imaging of exosome treatment for ischemic peripheral nerve damage.Experimental results confirmed that QDs can be used for non-invasive in vitro labeling of exosomes,with QDs-ADSC-Exos exhibiting strong fluorescence signals in the NIR-II window and demonstrating favorable NIR-II imaging characteristics in vivo.Notably,QDsADSC-Exos showed accumulation at the site of nerve injury in cases of ischemic peripheral nerve damage.Func⁃tional neurological assessments indicated that QDs-ADSC-Exos effectively promoted neural regeneration.This study highlights the potential of exosomes in treating ischemic peripheral nerve injuries and elucidates the spatio⁃temporal characteristics of exosome therapy,providing objective evidence for the further optimization of exosomebased treatment protocols.
文摘在法医学检案中,经常要对尸体上的损伤进行检验以确定生前伤或死后伤,并要鉴定损伤时间.损伤超过8小时者一般用常规组织学方法可以解决,但损伤后经过时间较短,单用组织学方法不能解决,如利用酶活性反应方法和利用在组织内细胞内进行特异、灵敏、稳定的抗原抗体反应,可以较准确鉴定损伤时间.Perper J. A. Raek Allio、Tanaka、Fatteh等报导用组织化学方法鉴定损伤时间,祝家镇、刘世仓教授近年来也有报道,但用免疫组化学方法来鉴定损伤后早期时间,就作者所知,国内、外未见报道,而FN在创伤愈合过程中的作用国内外已有报道.
文摘In order to explore the effect of electroacupuncture on rats with acute spinal injury,we established animal model of the spinal injury.Total proteins were extracted from spinal cord of normal rats,acute spinal injury rats and acute spinal injury rat treated by electroacupuncture,and separated by 2-DE.10 differential expressed proteins were identified by the MALDI-TOF-TOF-MS with high mass accuracy,high mascot scores and high reliability.
