Biosensors, which are the products of the biotechnology industry, are among the key projects of the 7th, 8th, and 9th Fiveyear Plans of China Science ＆ Technology Developing Programs, respectively, and they play an i...Biosensors, which are the products of the biotechnology industry, are among the key projects of the 7th, 8th, and 9th Fiveyear Plans of China Science ＆ Technology Developing Programs, respectively, and they play an important role in developing and reforming traditional biotechnology. SBA series biosensor analyzer, as the only one commercial biosensor in China, has attracted lots of attention in the process of information gathering and measurement for biological industry with the development of technology and society. In this paper, we presented an overview of the most important contributions dealing with the monitoring of the biochemical analytes in fermentation processes using SBA series biosensor analyzers in China. Future trends of the biosensor analyzer in China were also mentioned in the last section.展开更多
Optimal glucose feed strategy for glycerol fed-batch fermentation was investigated by Pontryagin’s maximum principle to maximize the final glycerol yield. The problem was solved by a nonsingular control approach by s...Optimal glucose feed strategy for glycerol fed-batch fermentation was investigated by Pontryagin’s maximum principle to maximize the final glycerol yield. The problem was solved by a nonsingular control approach by selecting the culture volume as the control variable, then the general optimal feed profile was numerically determined.展开更多
为提高海洋来源成团泛菌(Pantoea agglomerans)Y-03产低温甾醇酯酶的酶活力,该研究首先通过单因素试验及Plackett-Burman(PB)试验确定成团泛菌Y-03产低温甾醇酯酶酶活的显著影响因素,然后运用Box-Behnken(BB)响应面试验对其发酵工艺进...为提高海洋来源成团泛菌(Pantoea agglomerans)Y-03产低温甾醇酯酶的酶活力,该研究首先通过单因素试验及Plackett-Burman(PB)试验确定成团泛菌Y-03产低温甾醇酯酶酶活的显著影响因素,然后运用Box-Behnken(BB)响应面试验对其发酵工艺进行优化。结果表明,成团泛菌Y-03产低温甾醇酯酶的最适发酵工艺为葡萄糖添加量3.7%、胰蛋白胨添加量1.3%、K_(2)HPO_(4)添加量0.4%、初始p H值8、转速180 r/min、接种量4%、装液量44 m L/250 m L、发酵温度27℃。在此优化条件下,甾醇酯酶活力为484.24 U/L,相比于优化前酶活力提高143.89%。该研究为提高成团泛菌的甾醇酯酶产量及酶活力和未来放大生产研究奠定了基础。展开更多
基金Supported by the Postdoctoral Innovation Fund of Shandong Province(201303032)the Independent Innovation Projects of Shandong Province(2012CX20505)the National 863 High Technology Project of the Ministry of Science and Technology of China(2012AA021201)
文摘Biosensors, which are the products of the biotechnology industry, are among the key projects of the 7th, 8th, and 9th Fiveyear Plans of China Science ＆ Technology Developing Programs, respectively, and they play an important role in developing and reforming traditional biotechnology. SBA series biosensor analyzer, as the only one commercial biosensor in China, has attracted lots of attention in the process of information gathering and measurement for biological industry with the development of technology and society. In this paper, we presented an overview of the most important contributions dealing with the monitoring of the biochemical analytes in fermentation processes using SBA series biosensor analyzers in China. Future trends of the biosensor analyzer in China were also mentioned in the last section.
基金From National Ninth Five Years Project (NO. 96-03-03-03A).
文摘Optimal glucose feed strategy for glycerol fed-batch fermentation was investigated by Pontryagin’s maximum principle to maximize the final glycerol yield. The problem was solved by a nonsingular control approach by selecting the culture volume as the control variable, then the general optimal feed profile was numerically determined.
文摘为提高海洋来源成团泛菌(Pantoea agglomerans)Y-03产低温甾醇酯酶的酶活力,该研究首先通过单因素试验及Plackett-Burman(PB)试验确定成团泛菌Y-03产低温甾醇酯酶酶活的显著影响因素,然后运用Box-Behnken(BB)响应面试验对其发酵工艺进行优化。结果表明,成团泛菌Y-03产低温甾醇酯酶的最适发酵工艺为葡萄糖添加量3.7%、胰蛋白胨添加量1.3%、K_(2)HPO_(4)添加量0.4%、初始p H值8、转速180 r/min、接种量4%、装液量44 m L/250 m L、发酵温度27℃。在此优化条件下,甾醇酯酶活力为484.24 U/L,相比于优化前酶活力提高143.89%。该研究为提高成团泛菌的甾醇酯酶产量及酶活力和未来放大生产研究奠定了基础。
