In order to research segmented diverters for aircraft lightning protection, a transient 2 D multiphysics model based on magnetohydrodynamics theory is proposed to predict the location of the arc plasma discharge and l...In order to research segmented diverters for aircraft lightning protection, a transient 2 D multiphysics model based on magnetohydrodynamics theory is proposed to predict the location of the arc plasma discharge and lightning channel, and to simulate the electrothermal behavior.Based on numerical calculation and preliminary analysis, factors that affect the breakdown voltage of the segmented diverter are discussed. The results show that the voltage increase rate of the voltage source, the width of the air gap between metal segments and the geometry of these segments influence the breakdown voltage of the strip. High-voltage tests of the segmented diverter are performed to reveal air breakdown of the strip and redirect the lightning current.Experimental and numerical results are compared to verify the correctness of the numerical model. The ionization of the air gap between metal segments and the breakdown voltage of the strip calculated by the model are qualitatively consistent with experimental results. The breakdown voltage of the segmented diverter is far lower than the lightning voltage. When a lightning strike occurs, the segmented diverter can be quickly ionized to form a plasma channel which can guide the lightning current well.展开更多
Traditional transgenic detection methods require high test conditions and struggle to be both sensitive and efficient.In this study,a one-tube dual recombinase polymerase amplification(RPA)reaction system for CP4-EPSP...Traditional transgenic detection methods require high test conditions and struggle to be both sensitive and efficient.In this study,a one-tube dual recombinase polymerase amplification(RPA)reaction system for CP4-EPSPS and Cry1Ab/Ac was proposed and combined with a lateral flow immunochromatographic assay,named“Dual-RPA-LFD”,to visualize the dual detection of genetically modified(GM)crops.In which,the herbicide tolerance gene CP4-EPSPS and the insect resistance gene Cry1Ab/Ac were selected as targets taking into account the current status of the most widespread application of insect resistance and herbicide tolerance traits and their stacked traits.Gradient diluted plasmids,transgenic standards,and actual samples were used as templates to conduct sensitivity,specificity,and practicality assays,respectively.The constructed method achieved the visual detection of plasmid at levels as low as 100 copies,demonstrating its high sensitivity.In addition,good applicability to transgenic samples was observed,with no cross-interference between two test lines and no influence from other genes.In conclusion,this strategy achieved the expected purpose of simultaneous detection of the two popular targets in GM crops within 20 min at 37°C in a rapid,equipmentfree field manner,providing a new alternative for rapid screening for transgenic assays in the field.展开更多
呕吐毒素(deoxynivalenol,DON)对人类和动物的健康以及食品经济构成了极大的威胁。该研究建立一种基于金纳米花(gold nanoflowers,AuNFs)的免疫层析试纸条(immunochromatographic test strip,ICTS)快速检测玉米中的呕吐毒素的方法。采...呕吐毒素(deoxynivalenol,DON)对人类和动物的健康以及食品经济构成了极大的威胁。该研究建立一种基于金纳米花(gold nanoflowers,AuNFs)的免疫层析试纸条(immunochromatographic test strip,ICTS)快速检测玉米中的呕吐毒素的方法。采用种子生长法制备了AuNFs,将其与抗DON单克隆抗体结合制备免疫层析试纸条中的金标探针,并对pH值、抗体量、上样缓冲液浓度、检测线(test-line,T线)浓度和金标探针量进行优化,最终用于玉米中的DON检测。经过优化实验条件,确定了最优pH值为7.5,最佳抗体添加量为1μL,最适上样缓冲液浓度为PBST+1%PVP K30,最好的T线质量浓度为0.1 mg/mL以及最佳探针量为3μL。在最优反应体系下,实验结果可以通过肉眼观察进行定性分析,该方法检出限为20μg/kg,灵敏度≥99%、假阳性率≤1%、假阴性率≤1%,且与现有标准方法检测结果一致,同时与其他真菌毒素无交叉反应。以AuNFs标记抗体为探针制备的ICTS灵敏度高,特异性强,可以实现玉米中DON的快速检测。展开更多
基金supported by National Natural Science Foundation of China (No. 51475369)the Natural Science Basic Research Plan in Shaanxi Province of China (No. 2018JM1001)
文摘In order to research segmented diverters for aircraft lightning protection, a transient 2 D multiphysics model based on magnetohydrodynamics theory is proposed to predict the location of the arc plasma discharge and lightning channel, and to simulate the electrothermal behavior.Based on numerical calculation and preliminary analysis, factors that affect the breakdown voltage of the segmented diverter are discussed. The results show that the voltage increase rate of the voltage source, the width of the air gap between metal segments and the geometry of these segments influence the breakdown voltage of the strip. High-voltage tests of the segmented diverter are performed to reveal air breakdown of the strip and redirect the lightning current.Experimental and numerical results are compared to verify the correctness of the numerical model. The ionization of the air gap between metal segments and the breakdown voltage of the strip calculated by the model are qualitatively consistent with experimental results. The breakdown voltage of the segmented diverter is far lower than the lightning voltage. When a lightning strike occurs, the segmented diverter can be quickly ionized to form a plasma channel which can guide the lightning current well.
基金supported by the Scientific and Innovative Action Plan of Shanghai(21N31900800)Shanghai Rising-Star Program(23QB1403500)+4 种基金the Shanghai Sailing Program(20YF1443000)Shanghai Science and Technology Commission,the Belt and Road Project(20310750500)Talent Project of SAAS(2023-2025)Runup Plan of SAAS(ZP22211)the SAAS Program for Excellent Research Team(2022(B-16))。
文摘Traditional transgenic detection methods require high test conditions and struggle to be both sensitive and efficient.In this study,a one-tube dual recombinase polymerase amplification(RPA)reaction system for CP4-EPSPS and Cry1Ab/Ac was proposed and combined with a lateral flow immunochromatographic assay,named“Dual-RPA-LFD”,to visualize the dual detection of genetically modified(GM)crops.In which,the herbicide tolerance gene CP4-EPSPS and the insect resistance gene Cry1Ab/Ac were selected as targets taking into account the current status of the most widespread application of insect resistance and herbicide tolerance traits and their stacked traits.Gradient diluted plasmids,transgenic standards,and actual samples were used as templates to conduct sensitivity,specificity,and practicality assays,respectively.The constructed method achieved the visual detection of plasmid at levels as low as 100 copies,demonstrating its high sensitivity.In addition,good applicability to transgenic samples was observed,with no cross-interference between two test lines and no influence from other genes.In conclusion,this strategy achieved the expected purpose of simultaneous detection of the two popular targets in GM crops within 20 min at 37°C in a rapid,equipmentfree field manner,providing a new alternative for rapid screening for transgenic assays in the field.
