In order to research the influence of liquid water content ( LWC ) on blade icing of wind turbine, a numerical simulation method for blade icing was established. The numerical simulation was based on low speed viscous...In order to research the influence of liquid water content ( LWC ) on blade icing of wind turbine, a numerical simulation method for blade icing was established. The numerical simulation was based on low speed viscous N-S equation. The trajectory equation of water droplets was established by Lagrangian method. The mass and energy conservation equations of the water droplets impacting on the surface of the blade were solved based on control body theory. Three sections along blade span wise of a 1.5 MW wind turbine were decided to simulate icing. Five kinds of LWC were selected for simulation including 0.2,0.4,0.6,0.8 and 1.0 g/m^3 under two ambient temperatures of -10 ℃ and -20 ℃. The medium volume droplet diameter ( MVD ) was 30μm. The simulations included icing shape on blade surface, dimensionless icing area and dimensionless maximum stagnation thickness. Furthermore, the flow fields around both the iced blade airfoil and the original one were simulated and analyzed. Accor-ding to the results, the typical icing characteristics of icing shape, icing area and thickness were greatly affected by the difference of LWCs. This study can provide theoretical reference for the research on antiicing and deicing of wind turbine blade.展开更多
Objective:To anatomically and phenotypically characterize the insular cortex(IC)-nucleus tractus soli-tari(NTS)neural pathway.Methods:Adult male Sprague-Dawley rats were divided into three experimental cohorts for neu...Objective:To anatomically and phenotypically characterize the insular cortex(IC)-nucleus tractus soli-tari(NTS)neural pathway.Methods:Adult male Sprague-Dawley rats were divided into three experimental cohorts for neural circuit tracing.Anterograde labeling was achieved by injecting anterograde self-complementary adeno-associated viruses(scAAVs)into the IC.Retrograde tracing involved NTS injections of either retrograde scAAVs or FluoroGold(FG),combined with immunofluorescence histochemical staining to identify IC-originating projection neurons.For postsynaptic neurochemical phenotype characterization,IC was injected with AAV2/1-CaMKII-Cre,while a mixture of AAV2/9-Syn-DIO-mCherry and AAV2/9-VGAT1-EGFP was injected into the NTS.The rats were allowed to survive for one week following scAAVs or FG injection or four weeks after recombinase-dependent systems injection.Then the rats were sacrificed,and serial brain sections were prepared for immunofluorescence histochemical staining(brain section containing FG)and subsequent fluorescence/confocal microscopic analysis.Results:(1)Anterograde viral tracing re-vealed dense axonal terminals from the IC projecting to the medial subnucleus of the NTS,while retrograde tracing re-vealed that IC neurons projecting to the NTS were predominantly localized within the dysgranular layer;(2)IC-NTS projection neurons were exclusive glutamatergic(100%,n=3);(3)NTS neurons receiving IC inputs were mainly lo-calized in the medial subnucleus,and were predominantly GABAergic(79.8±3.2%,n=3).Conclusion:The pres-ent results indicate that a descending pathway from excitatory neurons of the IC terminates onto inhibitory neurons of the NTS,which might represent a potential neuromodulatory target for visceral pain disorders.展开更多
基金sponsored by the projects supported by the National Natural Science Foundation of China(NSFC,No.51576037 and 11172314)the National Key Basic Research Program of China (2015CB755800)
文摘In order to research the influence of liquid water content ( LWC ) on blade icing of wind turbine, a numerical simulation method for blade icing was established. The numerical simulation was based on low speed viscous N-S equation. The trajectory equation of water droplets was established by Lagrangian method. The mass and energy conservation equations of the water droplets impacting on the surface of the blade were solved based on control body theory. Three sections along blade span wise of a 1.5 MW wind turbine were decided to simulate icing. Five kinds of LWC were selected for simulation including 0.2,0.4,0.6,0.8 and 1.0 g/m^3 under two ambient temperatures of -10 ℃ and -20 ℃. The medium volume droplet diameter ( MVD ) was 30μm. The simulations included icing shape on blade surface, dimensionless icing area and dimensionless maximum stagnation thickness. Furthermore, the flow fields around both the iced blade airfoil and the original one were simulated and analyzed. Accor-ding to the results, the typical icing characteristics of icing shape, icing area and thickness were greatly affected by the difference of LWCs. This study can provide theoretical reference for the research on antiicing and deicing of wind turbine blade.
文摘Objective:To anatomically and phenotypically characterize the insular cortex(IC)-nucleus tractus soli-tari(NTS)neural pathway.Methods:Adult male Sprague-Dawley rats were divided into three experimental cohorts for neural circuit tracing.Anterograde labeling was achieved by injecting anterograde self-complementary adeno-associated viruses(scAAVs)into the IC.Retrograde tracing involved NTS injections of either retrograde scAAVs or FluoroGold(FG),combined with immunofluorescence histochemical staining to identify IC-originating projection neurons.For postsynaptic neurochemical phenotype characterization,IC was injected with AAV2/1-CaMKII-Cre,while a mixture of AAV2/9-Syn-DIO-mCherry and AAV2/9-VGAT1-EGFP was injected into the NTS.The rats were allowed to survive for one week following scAAVs or FG injection or four weeks after recombinase-dependent systems injection.Then the rats were sacrificed,and serial brain sections were prepared for immunofluorescence histochemical staining(brain section containing FG)and subsequent fluorescence/confocal microscopic analysis.Results:(1)Anterograde viral tracing re-vealed dense axonal terminals from the IC projecting to the medial subnucleus of the NTS,while retrograde tracing re-vealed that IC neurons projecting to the NTS were predominantly localized within the dysgranular layer;(2)IC-NTS projection neurons were exclusive glutamatergic(100%,n=3);(3)NTS neurons receiving IC inputs were mainly lo-calized in the medial subnucleus,and were predominantly GABAergic(79.8±3.2%,n=3).Conclusion:The pres-ent results indicate that a descending pathway from excitatory neurons of the IC terminates onto inhibitory neurons of the NTS,which might represent a potential neuromodulatory target for visceral pain disorders.
