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改进Hybrid A*算法的履带车辆路径规划
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作者 陈漫 谭赟璐 +3 位作者 于亮 刘宇键 王浩东 张小鹏 《北京理工大学学报》 北大核心 2025年第8期807-814,共8页
针对非结构化道路场景下,二维栅格地图规划的路径不适用于履带车辆的问题,利用Hybrid A*算法进行改进.设定自适应步长和自适应RS曲线半径,改变Hybrid A*节点扩展方法,根据节点所在位置及周围障碍物情况实现步长和RS曲线半径的动态扩展,... 针对非结构化道路场景下,二维栅格地图规划的路径不适用于履带车辆的问题,利用Hybrid A*算法进行改进.设定自适应步长和自适应RS曲线半径,改变Hybrid A*节点扩展方法,根据节点所在位置及周围障碍物情况实现步长和RS曲线半径的动态扩展,提高路径搜索的效率和灵活性;引入坡度代价函数,并对原代价函数进行改进,使路径避开地形起伏较大的区域,降低车辆运动过程中的俯仰和侧倾变化,减小路径的转向操作;通过梯度下降法对路径进行平滑处理,保证路径不与障碍物发生碰撞的同时降低曲率的变化,提高路径质量.经过仿真验证表明:自适应步长Hybrid A*-自适应RS曲线半径的路径规划算法可获得更短和转向更平滑的路径,具有较高的效率和灵活性;考虑坡度、转向的代价函数可有效降低路径在非结构化道路下的起伏,更利于车辆的跟踪和控制. 展开更多
关键词 履带车辆 路径规划 A*混合算法 RS曲线
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基于Real-time PCR法检测乳粉中牛源性成分定量研究
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作者 陈晨 史国华 +5 位作者 陈勃旭 张瑞 王玉欣 贾文珅 陈佳 周巍 《粮油食品科技》 CAS CSCD 北大核心 2024年第2期159-164,共6页
基于Real-timePCR建立了乳粉中牛源性成分相对定量检测方法,并对牛的特异性引物与探针进行了特异性、灵敏度和稳定性测试。通过模拟不同浓度牛乳粉与马乳粉混合样本,根据其△Ct值的函数关系进行线性拟合进而绘制标准曲线,建立乳粉中牛... 基于Real-timePCR建立了乳粉中牛源性成分相对定量检测方法,并对牛的特异性引物与探针进行了特异性、灵敏度和稳定性测试。通过模拟不同浓度牛乳粉与马乳粉混合样本,根据其△Ct值的函数关系进行线性拟合进而绘制标准曲线,建立乳粉中牛源性成分的相对定量检测。结果显示,该方法的最低检测限为0.00001 mg/mL,回收率为91.11%~119.2%,组间变异系数≤0.58%、组内变异系数≤1.44%。说明该方法在特异性与稳定性上适用于乳粉中牛源性成分及含量的掺假检测。 展开更多
关键词 牛乳粉 马乳粉 real-time PCR 掺假检测
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A Framework of LSTM Neural Network Model in Multi-Time Scale Real-Time Prediction of Ship Motions in Head Waves 被引量:1
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作者 CHEN Zhan-yang ZHAN Zheng-yong +2 位作者 CHANG Shao-ping XU Shao-feng LIU Xing-yun 《船舶力学》 EI CSCD 北大核心 2024年第12期1803-1819,共17页
Ship motions induced by waves have a significant impact on the efficiency and safety of offshore operations.Real-time prediction of ship motions in the next few seconds plays a crucial role in performing sensitive act... Ship motions induced by waves have a significant impact on the efficiency and safety of offshore operations.Real-time prediction of ship motions in the next few seconds plays a crucial role in performing sensitive activities.However,the obvious memory effect of ship motion time series brings certain difficulty to rapid and accurate prediction.Therefore,a real-time framework based on the Long-Short Term Memory(LSTM)neural network model is proposed to predict ship motions in regular and irregular head waves.A 15000 TEU container ship model is employed to illustrate the proposed framework.The numerical implementation and the real-time ship motion prediction in irregular head waves corresponding to the different time scales are carried out based on the container ship model.The related experimental data were employed to verify the numerical simulation results.The results show that the proposed method is more robust than the classical extreme short-term prediction method based on potential flow theory in the prediction of nonlinear ship motions. 展开更多
关键词 deep learning LSTM ship motion real-time prediction irregular waves
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Redox mechanism of geobattery and related electrical signals using a novel real-time self-potential monitoring experimental platform 被引量:2
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作者 XIE Jing CUI Yi-an +4 位作者 ZHANG Li-juan GUO You-jun CHEN Hang ZHANG Peng-fei LIU Jian-xin 《Journal of Central South University》 CSCD 2024年第11期4155-4173,共19页
Controlled laboratory experiments are proved to be a valuable tool for investigating changes in underground physical properties and the related response of surface geophysical signals.The self-potential(SP)method is w... Controlled laboratory experiments are proved to be a valuable tool for investigating changes in underground physical properties and the related response of surface geophysical signals.The self-potential(SP)method is widely used in mineral resource exploration due to its direct correlation with underground electrochemical gradients.This paper presented the design and construction of an experimental platform based on a multi-channel SP monitoring system.The proposed platform was used to monitor the anodizing corrosion process of different metal blocks from a laboratory perspective,record the real-time SP signal generated by the redox reaction,as well as investigate the geobattery mechanism associated with the natural polarization process of metal mineral resources.The experimental results demonstrate that the constructed SP monitoring platform effectively captures time-series SP signals and provides direct laboratory evidence for the geobattery model.The measured SP data were quantitatively interpreted using the simulated annealing algorithm,and the inversion results closely match the real model.This finding highlights the potential of the SP method as a promising tool for determining the location and spatial distribution of underground polarizers.The study holds reference value for the exploration and exploitation of mineral resources in both terrestrial and marine environments. 展开更多
关键词 SELF-POTENTIAL real-time monitoring laboratory experiment geobattery mechanism quantitative inversion
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一种基于real-time PCR技术的TTV检测方法的建立及应用
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作者 贾毅博 王高玉 +4 位作者 邓宛心 林彩云 杨华 陈运春 尹飞飞 《海南医学院学报》 CAS 北大核心 2024年第7期489-497,共9页
目的:本研究旨在开发一种具有更高灵敏度和特异性的TTV检测技术,为揭示TTV在多种疾病过程中的作用提供重要的技术支持。方法:为了更精确、灵敏的检测TTV,本研究分析了目前公布的所有亚型的TTV基因序列,在此基础上建立了一种基于UTR区域... 目的:本研究旨在开发一种具有更高灵敏度和特异性的TTV检测技术,为揭示TTV在多种疾病过程中的作用提供重要的技术支持。方法:为了更精确、灵敏的检测TTV,本研究分析了目前公布的所有亚型的TTV基因序列,在此基础上建立了一种基于UTR区域的real-time PCR检测方法,并与文献报道应用较为广泛的PCR检测方法进行了对比。结果:本研究建立的方法在1×10^(7)~1×10^(1) copies/μL标准品浓度范围内具有良好的线性关系,相关系数为1.000,斜率为-3.446,检测下限为1×10^(1) copies/μL。重复性试验结果显示,组内变异系数为7.22%,表明本方法重复性、稳定性较强。针对30份临床样本,使用本研究建立的real-time PCR检测方法及目前被多个研究所使用的4套引物进行对比。结果表明,本研究所建立的方法灵敏度显著高于文献中报道的4种方法(P<0.01);Sanger测序结果表明,本方法检测出的30份阳性样本均为TTV,检测特异性为100%。结论:本研究采用基于TaqMan探针的real-time PCR检测方法,检测灵敏性高、覆盖基因型范围广,尤其对于TTV病毒载量较低的情况下能够进行定量检测,对于TTV病毒的致病性及作为免疫标志物的应用提供重要的技术支持。 展开更多
关键词 Torque teno virus 基因组扩增测序 real-time PCR检测
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基于JPS和变半径RS曲线的Hybrid A^(*)路径规划算法
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作者 张博强 张成龙 +1 位作者 冯天培 高向川 《郑州大学学报(工学版)》 北大核心 2025年第2期19-25,共7页
为解决混合A^(*)(Hybrid A^(*))算法在高分辨率地图和复杂场景下搜索效率低、耗费时间长的问题,通过对影响传统Hybrid A^(*)算法搜索效率的因素进行分析,提出了J-Hybrid A^(*)算法。首先,在Hybrid A^(*)算法扩展节点前,使用跳点搜索(JPS... 为解决混合A^(*)(Hybrid A^(*))算法在高分辨率地图和复杂场景下搜索效率低、耗费时间长的问题,通过对影响传统Hybrid A^(*)算法搜索效率的因素进行分析,提出了J-Hybrid A^(*)算法。首先,在Hybrid A^(*)算法扩展节点前,使用跳点搜索(JPS)算法进行起点到终点的路径搜索,将该路径进行拉直处理后作为计算节点启发值的基础;其次,设计了新的启发函数,在Hybrid A^(*)算法扩展前就能完成所有节点启发值的计算,减少了Hybrid A^(*)扩展节点时计算启发值所需的时间;最后,将RS曲线由最小转弯半径搜索改为变半径RS曲线搜索,使RS曲线能够更早搜索到一条无碰撞路径,进一步提升了Hybrid A^(*)算法的搜索效率。仿真结果表明:所提J-Hybrid A^(*)算法在简单环境中比传统Hybrid A^(*)算法和反向Hybrid A^(*)算法用时分别缩短68%、21%,在复杂环境中缩短59%、27%。在不同分辨率地图场景中,随着地图分辨率的提高,规划效率显著提升。实车实验表明:所提J-Hybrid A^(*)算法相较于传统Hybrid A^(*)算法和反向Hybrid A^(*)算法的搜索用时分别减少88%、82%,有效提升了Hybrid A^(*)算法的搜索效率、缩短了路径规划所需时间。 展开更多
关键词 hybrid A^(*)算法 启发函数 JPS算法 RS曲线 路径规划
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Real-Time Monitoring Method for Cow Rumination Behavior Based on Edge Computing and Improved MobileNet v3
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作者 ZHANG Yu LI Xiangting +4 位作者 SUN Yalin XUE Aidi ZHANG Yi JIANG Hailong SHEN Weizheng 《智慧农业(中英文)》 CSCD 2024年第4期29-41,共13页
[Objective]Real-time monitoring of cow ruminant behavior is of paramount importance for promptly obtaining relevant information about cow health and predicting cow diseases.Currently,various strategies have been propo... [Objective]Real-time monitoring of cow ruminant behavior is of paramount importance for promptly obtaining relevant information about cow health and predicting cow diseases.Currently,various strategies have been proposed for monitoring cow ruminant behavior,including video surveillance,sound recognition,and sensor monitoring methods.How‐ever,the application of edge device gives rise to the issue of inadequate real-time performance.To reduce the volume of data transmission and cloud computing workload while achieving real-time monitoring of dairy cow rumination behavior,a real-time monitoring method was proposed for cow ruminant behavior based on edge computing.[Methods]Autono‐mously designed edge devices were utilized to collect and process six-axis acceleration signals from cows in real-time.Based on these six-axis data,two distinct strategies,federated edge intelligence and split edge intelligence,were investigat‐ed for the real-time recognition of cow ruminant behavior.Focused on the real-time recognition method for cow ruminant behavior leveraging federated edge intelligence,the CA-MobileNet v3 network was proposed by enhancing the MobileNet v3 network with a collaborative attention mechanism.Additionally,a federated edge intelligence model was designed uti‐lizing the CA-MobileNet v3 network and the FedAvg federated aggregation algorithm.In the study on split edge intelli‐gence,a split edge intelligence model named MobileNet-LSTM was designed by integrating the MobileNet v3 network with a fusion collaborative attention mechanism and the Bi-LSTM network.[Results and Discussions]Through compara‐tive experiments with MobileNet v3 and MobileNet-LSTM,the federated edge intelligence model based on CA-Mo‐bileNet v3 achieved an average Precision rate,Recall rate,F1-Score,Specificity,and Accuracy of 97.1%,97.9%,97.5%,98.3%,and 98.2%,respectively,yielding the best recognition performance.[Conclusions]It is provided a real-time and effective method for monitoring cow ruminant behavior,and the proposed federated edge intelligence model can be ap‐plied in practical settings. 展开更多
关键词 cow rumination behavior real-time monitoring edge computing improved MobileNet v3 edge intelligence model Bi-LSTM
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Hybrid fault tolerance in distributed in-memory storage systems
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作者 Zheng Gong Si Wu Yinlong Xu 《中国科学技术大学学报》 北大核心 2025年第1期59-68,58,I0002,共12页
An in-memory storage system provides submillisecond latency and improves the concurrency of user applications by caching data into memory from external storage.Fault tolerance of in-memory storage systems is essential... An in-memory storage system provides submillisecond latency and improves the concurrency of user applications by caching data into memory from external storage.Fault tolerance of in-memory storage systems is essential,as the loss of cached data requires access to data from external storage,which evidently increases the response latency.Typically,replication and erasure code(EC)are two fault-tolerant schemes that pose different trade-offs between access performance and storage usage.To help make the best performance and space trade-off,we design ElasticMem,a hybrid fault-tolerant distributed in-memory storage system that supports elastic redundancy transition to dynamically change the fault-tolerant scheme.ElasticMem exploits a novel EC-oriented replication(EOR)that carefully designs the data placement of replication according to the future data layout of EC to enhance the I/O efficiency of redundancy transition.ElasticMem solves the consistency problem caused by concurrent data accesses via a lightweight table-based scheme combined with data bypassing.It detects correlated read and write requests and serves subsequent read requests with local data.We implement a prototype that realizes ElasticMem based on Memcached.Experiments show that ElasticMem remarkably reduces the time of redundancy transition,the overall latency of correlated concurrent data accesses,and the latency of single data access among them. 展开更多
关键词 in-memory storage system hybrid fault tolerance replication erasure code
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A review of carbon-based hybrid materials for supercapacitors
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作者 Theodore Azemtsop Manfo Hannu Laaksonen 《新型炭材料(中英文)》 北大核心 2025年第1期81-110,共30页
Supercapacitors are gaining popularity due to their high cycling stability,power density,and fast charge and discharge rates.Researchers are ex-ploring electrode materials,electrolytes,and separat-ors for cost-effecti... Supercapacitors are gaining popularity due to their high cycling stability,power density,and fast charge and discharge rates.Researchers are ex-ploring electrode materials,electrolytes,and separat-ors for cost-effective energy storage systems.Ad-vances in materials science have led to the develop-ment of hybrid nanomaterials,such as combining fil-amentous carbon forms with inorganic nanoparticles,to create new charge and energy transfer processes.Notable materials for electrochemical energy-stor-age applications include MXenes,2D transition met-al carbides,and nitrides,carbon black,carbon aerogels,activated carbon,carbon nanotubes,conducting polymers,carbon fibers,and nanofibers,and graphene,because of their thermal,electrical,and mechanical properties.Carbon materials mixed with conducting polymers,ceramics,metal oxides,transition metal oxides,metal hydroxides,transition metal sulfides,trans-ition metal dichalcogenide,metal sulfides,carbides,nitrides,and biomass materials have received widespread attention due to their remarkable performance,eco-friendliness,cost-effectiveness,and renewability.This article explores the development of carbon-based hybrid materials for future supercapacitors,including electric double-layer capacitors,pseudocapacitors,and hy-brid supercapacitors.It investigates the difficulties that influence structural design,manufacturing(electrospinning,hydro-thermal/solvothermal,template-assisted synthesis,electrodeposition,electrospray,3D printing)techniques and the latest car-bon-based hybrid materials research offer practical solutions for producing high-performance,next-generation supercapacitors. 展开更多
关键词 Carbon-based hybrid material Structure design Electrode material Specific capacitance SUPERCAPACITORS
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3D printed hybrid rocket fuels with μAl core-shell particles coated with polyvinylidene fluoride and polydopamine: Enhanced combustion characteristics
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作者 Qihang Chen Xiaolong Fu +6 位作者 Weitao Yang Suhang Chen Zhiming Guo Rui Hu Huijie Zhang Lianpeng Cui Xu Xia 《Defence Technology(防务技术)》 2025年第4期59-70,共12页
3D printing technology enhances the combustion characteristics of hybrid rocket fuels by enabling complex geometries. However, improvements in regression rates and energy properties of monotonous 3D printed fuels have... 3D printing technology enhances the combustion characteristics of hybrid rocket fuels by enabling complex geometries. However, improvements in regression rates and energy properties of monotonous 3D printed fuels have been limited. This study explores the impact of poly(vinylidene fluoride) and polydopamine-coated aluminum particles on the thermal and combustion properties of 3D printed hybrid rocket fuels. Physical self-assembly and anti-solvent methods were employed for constructing composite μAl particles. Characterization using SEM, XRD, XPS, FTIR, and μCT revealed a core-shell structure and homogeneous elemental distribution. Thermal analysis showed that PVDF coatings significantly increased the heat of combustion for aluminum particles, with maximum enhancement observed in μAl@PDA@PVDF(denoted as μAl@PF) at 6.20 k J/g. Subsequently, 3D printed fuels with varying pure and composite μAl particle contents were prepared using 3D printing. Combustion tests indicated higher regression rates for Al@PF/Resin composites compared to pure resin, positively correlating with particle content. The fluorocarbon-alumina reaction during the combustion stage intensified Al particle combustion, reducing residue size. A comprehensive model based on experiments provides insights into the combustion process of PDA and PVDF-coated droplets. This study advances the design of 3D-printed hybrid rocket fuels, offering strategies to improve regression rates and energy release, crucial for enhancing solid fuel performance for hybrid propulsion. 展开更多
关键词 hybrid propulsion Regression rate 3D print fuels Micro aluminum CORE-SHELL mAl@PDA@PVDF
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Optimization of Infrared-microwave Post-processing Process for 3D Printed Raspberry Preserves Based on AHP-CRITIC Hybrid Weighting Combined with Response Surface Method
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作者 Zheng Xianzhe Song Ruonan +2 位作者 Cong Hongyue Zhang Yuhan Xue Liangliang 《Journal of Northeast Agricultural University(English Edition)》 2025年第1期27-44,共18页
In order to improve the quality of 3D printed raspberry preserves after post-processing,microwave ovens combining infrared and microwave methods were utilized.The effects of infrared heating temperature,infrared heati... In order to improve the quality of 3D printed raspberry preserves after post-processing,microwave ovens combining infrared and microwave methods were utilized.The effects of infrared heating temperature,infrared heating time,microwave power,microwave heating time on the center temperature,moisture content,the chroma(C*),the total color difference(ΔE*),shape fidelity,hardness,and the total anthocyanin content of 3D printed raspberry preserves were analyzed by response surface method(RSM).The results showed that under combining with the two methods,infrared heating improved the fidelity and quality degradation of printed products,while microwave heating enhanced the efficiency of infrared heating.Infrared-microwave combination cooking could maintain relatively stable color appearance and shape of 3D printed raspberry preserves.The AHP–CRITIC hybrid weighting method combined with the response surface test to determine the comprehensive weights of the evaluation indicators optimized the process parameters,and the optimal process parameters were obtained:infrared heating temperature of 190℃,infrared heating time of 10 min and 30 s,microwave power of 300 W,and microwave heating time of 2 min and 6 s.The 3D printed raspberry cooking methods obtained under the optimal conditions seldom had color variation,porous structure,uniform texture,and high shape fidelity,which retained the characteristics of personalized manufacturing by 3D printing.This study could provide a reference for the postprocessing and quality control of 3D cooking methods. 展开更多
关键词 3D printing RASPBERRY MICROWAVE infrared heating hybrid weighting response surface method
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Screening of metal additives in ABS polymer fuel for enhanced performance in hybrid rocket motors:A computational analysis using CEA
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作者 Gail Ndlovu Bilainu Oboirien Patrick Ndungu 《Defence Technology(防务技术)》 2025年第8期176-184,共9页
This study investigates the potential of metal additives in acrylonitrile butadiene styrene(ABS)polymer fuel to enhance hybrid rocket motor(HRM)performance through computational analysis,Chemical Equilibrium with Appl... This study investigates the potential of metal additives in acrylonitrile butadiene styrene(ABS)polymer fuel to enhance hybrid rocket motor(HRM)performance through computational analysis,Chemical Equilibrium with Applications(CEA),software.ABS was selected as the base fuel due to its thermoplastic nature,which allows for the creation of complex fuel geometries through 3D printing,offering significant flexibility in fuel design.Hybrid rockets,which combine a solid fuel with a liquid oxidiser,offer advantages in terms of operational simplicity and safety.However,conventional polymer fuels often exhibit low regression rates and suboptimal combustion efficiencies.In this research,we evaluated a range of metal additives-aluminium(Al),boron(B),nickel(Ni),copper(Cu),and iron(Fe)-at chamber pressures ranging from 1 to 30 bar and oxidiser-to-fuel(O/F)ratios between 1.1 and 12,resulting in 1800 unique test conditions.The main performance parameters used to assess each formulation were characteristic velocity(C^(*))and adiabatic flame temperature.The results revealed that each test produced a different optimum O/F ratio,with most ratios falling between 4 and 6.The highest performance was achieved at a chamber pressure of 30 bar across all formulations.Among the additives,Al and B demonstrated significant potential for improved combustion performance with increasing metal loadings.In contrast,Fe,Cu,and Ni reached optimal performance at a minimum loading of 1%.Future work includes investigating B-Al metal composites as additives into the ABS base polymer fuel,and doing experimental validation tests where the metallised ABS polymer fuel is 3D printed. 展开更多
关键词 hybrid rocket motors Acrylonitrile butadiene styrene(ABS) Metallised polymer fuels Combustion performance Characteristic velocity(C*) Chemical equilibrium with applications (CEA)
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运用Real-time PCR方法研究日粮添加豆油与胡麻油对肉牛瘤胃纤维分解菌数量的影响 被引量:13
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作者 李旦 王加启 +3 位作者 卜登攀 杨舒黎 魏宏阳 周凌云 《动物营养学报》 CAS CSCD 北大核心 2008年第3期256-260,共5页
本研究分别以产琥珀酸丝状杆菌(Fibrobacter succinogenes)、黄色瘤胃球菌(Ruminococcus flavefaciens)、白色瘤胃球菌(Ruminobacter albus)和溶纤维丁酸弧菌(Butyrivibrio fibrisolvens)16S rDNA序列设计引物,运用Real-ti me PCR技术... 本研究分别以产琥珀酸丝状杆菌(Fibrobacter succinogenes)、黄色瘤胃球菌(Ruminococcus flavefaciens)、白色瘤胃球菌(Ruminobacter albus)和溶纤维丁酸弧菌(Butyrivibrio fibrisolvens)16S rDNA序列设计引物,运用Real-ti me PCR技术研究日粮中添加豆油与胡麻油对肉牛上述4种瘤胃纤维分解菌数量的影响。结果表明,与对照组(CK)相比,添加豆油组(LOC1)和胡麻油(LOC2)组,产琥珀酸丝状杆菌(Fibrobacter succinogenes)、黄色瘤胃球菌(Ruminococcus flavefaciens)、白色瘤胃球菌(Ruminobacter albus)和溶纤维丁酸弧菌(Butyrivibrio fibrisol-vens)数量显著减少(P<0.05),分别降低了78%和31%、30%和36%、27%和23%、6%和13%。通过该方法的结果表明日粮中添加4%的豆油和胡麻油显著减少了瘤胃中纤维分解菌,对产琥珀酸丝状杆菌的影响明显。而且采用Real-ti me PCR方法对瘤胃纤维分解菌进行定量,可以快速有效反映出在日粮改变的情况下菌的数量变化趋势,相对于传统计数方法更直观、快捷与准确。 展开更多
关键词 real-time PCR 瘤胃纤维菌 定量 肉牛
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非洲猪瘟病毒常规PCR及Real-time PCR检测方法的建立 被引量:22
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作者 张泉 朱鸿飞 孙怀昌 《中国预防兽医学报》 CAS CSCD 北大核心 2007年第6期458-461,共4页
根据非洲猪瘟病毒(African swine fever virus,ASFV)P72基因的核苷酸序列,设计并合成引物以及荧光标记的TaqMan探针,以含P72基因的重组质粒作为阳性模板,用于常规PCR和Real-time PCR方法的建立,结果表明常规PCR的检测灵敏度是600个拷贝... 根据非洲猪瘟病毒(African swine fever virus,ASFV)P72基因的核苷酸序列,设计并合成引物以及荧光标记的TaqMan探针,以含P72基因的重组质粒作为阳性模板,用于常规PCR和Real-time PCR方法的建立,结果表明常规PCR的检测灵敏度是600个拷贝的病毒核酸分子,Real-time PCR的检测灵敏度是20个拷贝的病毒核酸分子,两种PCR检测方法均具有特异性强、简单快速的优点。可以用于出入境检验检疫部门对非洲猪瘟病毒的快速检测。 展开更多
关键词 非洲猪瘟 常规PCR real-time PCR
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一种莱姆病螺旋体real-time PCR方法的建立及其在鼠标本检测中的应用评价(英文) 被引量:13
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作者 耿震 侯学霞 +1 位作者 张琳 郝琴 《中国人兽共患病学报》 CAS CSCD 北大核心 2015年第9期812-816,共5页
目的基于莱姆病螺旋体recA基因,建立一种检测鼠中莱姆病螺旋体的real-time PCR方法。方法通过GenBank分析比较莱姆病螺旋体recA基因,选择其保守序列设计MGB探针及引物并进行方法学评估。并应用建立的real-time PCR方法和nested PCR方法... 目的基于莱姆病螺旋体recA基因,建立一种检测鼠中莱姆病螺旋体的real-time PCR方法。方法通过GenBank分析比较莱姆病螺旋体recA基因,选择其保守序列设计MGB探针及引物并进行方法学评估。并应用建立的real-time PCR方法和nested PCR方法对收集的123份鼠标本进行检测分析。结果本研究建立的real-time PCR方法仅对莱姆病螺旋体检测阳性,其最小检出浓度为101copies/μL。标准曲线各浓度点Ct值批内、批间平均变异系数(CV)分别为1.56%和2.30%。123份鼠标本中,real-time PCR检测59例阳性,nested PCR检测43例阳性。结论新建立的real-time PCR方法具有快速、敏感和特异的优点,可用于鼠标本中莱姆病螺旋体的检测。 展开更多
关键词 莱姆病螺旋体 real-time PCR nested PCR
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用DGGE和Real-Time PCR对低温沼气池中产甲烷古菌群落的研究 被引量:23
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作者 王彦伟 徐凤花 +3 位作者 阮志勇 宋金龙 王庆 赵斌 《中国沼气》 北大核心 2012年第1期8-12,共5页
利用DGGE及Real-Time PCR技术对不同地区低温沼气池中沼泥行进发酵前期、后期产甲烷古菌群落变化进行了研究。DGGE技术用于分析发酵前后产甲烷古菌优势群落的变化;Real-Time PCR技术用于分析发酵前期、后期样品产甲烷古菌的数量变化。... 利用DGGE及Real-Time PCR技术对不同地区低温沼气池中沼泥行进发酵前期、后期产甲烷古菌群落变化进行了研究。DGGE技术用于分析发酵前后产甲烷古菌优势群落的变化;Real-Time PCR技术用于分析发酵前期、后期样品产甲烷古菌的数量变化。结果表明,沼气低温发酵的过程中,不同沼泥样品发酵前期、后期产甲烷古菌的优势群落差异明显且数量上也存在较大差异;主要变化的产甲烷古菌的优势群落为甲烷粒菌属(Methanocor-pusculum),甲烷八叠球菌属(Methanosarcina),甲烷鬃毛菌属(Methanosaeta)。甲烷八叠球菌属(Methanosarcina)在黑龙江沼泥样品(A1)发酵后期成为优势群落,甲烷鬃毛菌属(Methanosaeta)在山东发酵前期(B1),后期样品(B2)及安徽发酵前期样品(C1)中占有绝对优势。产甲烷古菌的数量介于104~105个.mL-1之间,其中黑龙江样品中产甲烷菌数量最多,而安徽样品中产甲烷菌数量最少。 展开更多
关键词 沼泥 产甲烷古菌 DGGE real-time PCR
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Real-time PCR方法检测肉品中的沙门氏菌 被引量:16
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作者 方平 杨永莉 +1 位作者 杨宝 王晓闻 《山西农业科学》 2010年第8期71-76,共6页
应用SYBR Green I染料能选择性结合双链DNA的特点,可检测到沙门氏菌fimI基因特异性靶序列扩增所产生的荧光信号,通过熔解曲线可知其熔点值约为85.6℃,而对其他非沙门氏菌则检测不到荧光信号。建立了一种肉品中的沙门氏菌Real-time PCR... 应用SYBR Green I染料能选择性结合双链DNA的特点,可检测到沙门氏菌fimI基因特异性靶序列扩增所产生的荧光信号,通过熔解曲线可知其熔点值约为85.6℃,而对其他非沙门氏菌则检测不到荧光信号。建立了一种肉品中的沙门氏菌Real-time PCR检测方法,用该方法检测市售牛肉、香肠中的沙门氏菌,其检测灵敏度分别为13,12 cfu/25 g,从样品的处理到得出检验结果可以在10 h内完成。该检测方法具有简便、快速、特异性强、敏感度高等特点。 展开更多
关键词 沙门氏菌 real-time PCR 快速检测 肉品
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Real-time PCR技术的应用研究进展 被引量:6
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作者 李珊珊 王加启 +3 位作者 李旦 董晓丽 赵圣国 卜登攀 《生物技术通报》 CAS CSCD 北大核心 2009年第8期60-62,共3页
Real-time PCR(RT-PCR)是基于PCR,利用不同的荧光检测定量核酸的技术,广泛应用于基因分型,单核苷酸多态性,等位基因突变检测等方面。综述了RT-PCR作为一种检测技术,在医学、食品、环境微生物等不同领域的应用进展。
关键词 real-time PCR基因诊断 基因检测 基因表达
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Real-Time PCR探针法定量检测沙门菌方法的建立及应用 被引量:3
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作者 耿士忠 潘志明 +5 位作者 方强 丛秋霞 刘杰 刘志成 文志发 焦新安 《中国人兽共患病学报》 CAS CSCD 北大核心 2010年第11期1004-1007,1027,共5页
目的建立快速、特异性好、灵敏度高的Real-Time PCR方法定量检测沙门菌。方法根据编码沙门菌肠毒素基因stn的核苷酸序列,设计荧光探针和一对引物,通过对荧光定量PCR反应体系和反应条件的摸索,建立定量检测沙门菌的方法。结果建立的Real-... 目的建立快速、特异性好、灵敏度高的Real-Time PCR方法定量检测沙门菌。方法根据编码沙门菌肠毒素基因stn的核苷酸序列,设计荧光探针和一对引物,通过对荧光定量PCR反应体系和反应条件的摸索,建立定量检测沙门菌的方法。结果建立的Real-Ti me PCR方法有很好的特异性与敏感性,所检测沙门菌结果均为阳性,而非沙门菌均为阴性;标准曲线相关系数为R2=0.993,其敏感性为5CFU。运用该方法对108份鸡粪便、50份鸡肉以及58份水样进行检测,阳性率分别为3.7%(6/108)、4%(2/50)和3.4%(2/58),与传统细菌分离检测结果相符。结论结果表明该方法具有简便、快速、特异性强、敏感性高等特点,此研究为环境及疾病诊断中沙门菌快速检测提供了新方法。 展开更多
关键词 沙门菌 real-time PCR 荧光定量 快速检测
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猪圆环病毒2型SYBRGreen Real-time qPCR检测方法的建立 被引量:9
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作者 冯华 刘运超 +2 位作者 陈玉梅 魏蔷 张改平 《华北农学报》 CSCD 北大核心 2018年第4期98-103,共6页
为了建立一种基于SYBRGreen的猪圆环病毒2型(PCV2)荧光定量PCR诊断方法,以PCV2 ORF2基因序列为研究对象,构建p MD19T-ORF2重组质粒作为阳性标准品,设计特异性引物,对该方法的最适引物浓度、退火进行了优化,并对该方法的灵敏度、特异性... 为了建立一种基于SYBRGreen的猪圆环病毒2型(PCV2)荧光定量PCR诊断方法,以PCV2 ORF2基因序列为研究对象,构建p MD19T-ORF2重组质粒作为阳性标准品,设计特异性引物,对该方法的最适引物浓度、退火进行了优化,并对该方法的灵敏度、特异性、可重复性以及临床样品检出率进行了评价。结果表明,该方法引物的最佳终浓度为0. 2 mol/L,最适退火温度为55℃;检测限可达到3. 0×10~2个拷贝/mL,而普通PCR检测限在3. 0×10~4个拷贝/mL;溶解曲线分析显示,在77~79℃有单一的溶解峰出现,并与CSFV、PPV、PRRSV、PRV没有交叉反应;组内重复性变异系数为0. 29%~0. 32%,组间变异系数为0. 32%~0. 36%;此外,该方法对临床样品的检出率为81. 5%(31/38),而常规PCR为68. 4%(26/38)。因此,相比常规PCR,该方法更为快速、灵敏、特异、稳定,更适合PCV2临床样品的检测,可为该PCV2早期感染的快速检测提供新方法。 展开更多
关键词 猪圆环病毒2型 PCV2 ORF2 SYBRGreen real-time qPCR
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