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Symbiosis between fungi and the hybrid Cymbidium and its mycorrhizal microstructures 被引量:1
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作者 DONG Fang LIU Hong-xia +1 位作者 JIN Hui LUO Yi-bo 《Forestry Studies in China》 CAS 2008年第1期41-44,共4页
Tissue culture seedlings of the hybrid Cymbidium were inoculated with six different fungal strains, isolated from the roots of different wild terrestrial orchids. About three months later, the average increment of fre... Tissue culture seedlings of the hybrid Cymbidium were inoculated with six different fungal strains, isolated from the roots of different wild terrestrial orchids. About three months later, the average increment of fresh weight of seedlings inoculated with strains CF1, CF3 and CF12 were respectively 130.26%, 345.65% and 153.34% while that of the control was only 88.40%. The differences between the three treatments and the control were statistically significant (α = 0.05), highlighting the treatment with strain CF3 (α = 0.01). In addition, the three strains were obtained by re-isolating. Pelotons, regarded as typical structures of orchid mycorrhiza, were also found in the inoculating roots under a microscope. It seems that the strains of CF1, CF3, and CF12 are associated with the hybrid Cymbidium and supplied the orchid with nutrition. It can be confirmed that the three strains are beneficial for the seedlings of this hybrid. 展开更多
关键词 hybrid cymbidium mycorrhizal fungi symbiosis mycorrhizal structure
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基于抑制消减杂交技术筛选AM真菌与紫穗槐共生相关基因 被引量:2
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作者 宋福强 孔祥仕 +1 位作者 李季泽 常伟 《林业科学》 EI CAS CSCD 北大核心 2014年第11期64-74,共11页
利用抑制消减杂交技术筛选AM真菌在侵染紫穗槐的过程中紫穗槐差异表达的基因,共获得47个unigenes,其中35个显著匹配。根据GO分类信息、Blast注释信息和相关文献信息,显著匹配的35个unigenes基因按功能分为防御胁迫(24%)、物质代谢(17%)... 利用抑制消减杂交技术筛选AM真菌在侵染紫穗槐的过程中紫穗槐差异表达的基因,共获得47个unigenes,其中35个显著匹配。根据GO分类信息、Blast注释信息和相关文献信息,显著匹配的35个unigenes基因按功能分为防御胁迫(24%)、物质代谢(17%)、能量代谢(14%)、蛋白合成(11%)、信号转导(11%)、细胞结构构建(11%)、转录(6%)、蛋白折叠和降解(6%)8类。其中,胁迫防御相关基因和代谢相关基因所占比例最大,表明二者在AM真菌和紫穗槐共生互作过程中起重要作用。这些基因广泛参与生物体生命活动的各个过程,因此AM真菌与紫穗槐共生的过程是一个系统涉及各种生物过程的活动。 展开更多
关键词 紫穗槐 丛枝菌根真菌 抑制消减杂交技术 共生相关基因
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