芦荟素是芦荟叶中主要的蒽醌类成分,通常以芦荟素A和B两种非对映异构体形式并存,目前已成为许多芦荟产品质量控制指标之一。采用高速逆流色谱和硅胶柱色谱结合的方法,从老芦荟干粉中分离制备了高纯度的芦荟素A(纯度为98%)和芦荟素B(纯度...芦荟素是芦荟叶中主要的蒽醌类成分,通常以芦荟素A和B两种非对映异构体形式并存,目前已成为许多芦荟产品质量控制指标之一。采用高速逆流色谱和硅胶柱色谱结合的方法,从老芦荟干粉中分离制备了高纯度的芦荟素A(纯度为98%)和芦荟素B(纯度为96%)样品,并采用快原子轰击质谱(FAB-M S)和核磁共振氢谱(1H NMR)以及GOE SY(grad ien t-enhanced nuc lear O verhau ser e ffec t spec tro scopy)等方法对所得的两个芦荟素异构体的立体构象进行了确认。该法具有制备量大和分离效率高的特点。展开更多
Alkaloids from swertia mileensis were isolated successfully by high speed countercurrent chromatography with a two phase solvent system, CHCl 3∶CH 3OH∶NaH 2PO 4(pH=5.3)=4∶3∶1,to obtain 6 components,in which 3 comp...Alkaloids from swertia mileensis were isolated successfully by high speed countercurrent chromatography with a two phase solvent system, CHCl 3∶CH 3OH∶NaH 2PO 4(pH=5.3)=4∶3∶1,to obtain 6 components,in which 3 components exhibited orange red colour with bismuth potassium iodide reagent.The results were verified by TLC and six pure components were isolated.One was characterized as gentianine(28.5%) by spectroscopic method.展开更多
文摘芦荟素是芦荟叶中主要的蒽醌类成分,通常以芦荟素A和B两种非对映异构体形式并存,目前已成为许多芦荟产品质量控制指标之一。采用高速逆流色谱和硅胶柱色谱结合的方法,从老芦荟干粉中分离制备了高纯度的芦荟素A(纯度为98%)和芦荟素B(纯度为96%)样品,并采用快原子轰击质谱(FAB-M S)和核磁共振氢谱(1H NMR)以及GOE SY(grad ien t-enhanced nuc lear O verhau ser e ffec t spec tro scopy)等方法对所得的两个芦荟素异构体的立体构象进行了确认。该法具有制备量大和分离效率高的特点。
文摘Alkaloids from swertia mileensis were isolated successfully by high speed countercurrent chromatography with a two phase solvent system, CHCl 3∶CH 3OH∶NaH 2PO 4(pH=5.3)=4∶3∶1,to obtain 6 components,in which 3 components exhibited orange red colour with bismuth potassium iodide reagent.The results were verified by TLC and six pure components were isolated.One was characterized as gentianine(28.5%) by spectroscopic method.
