OBJECTIVE Forsythiae Fructus(Lianqiao)is a typical heat-clearing and detoxicating traditional Chinese medicine(TCM)herb,which has been traditionally used for treating cancer according to TCM theory.However,the underly...OBJECTIVE Forsythiae Fructus(Lianqiao)is a typical heat-clearing and detoxicating traditional Chinese medicine(TCM)herb,which has been traditionally used for treating cancer according to TCM theory.However,the underlying mechanism has not been fully explained.METHODS In this study,we investigated the antitumor effect of Forsythiae Fructus aqueous extract(FAE)on B16-F10 melanoma.RESULTS FAE strongly inhibited the tumor growth and metastasis formation in B16-F10 melanoma transplanted mice.The survival time of tumor-bearing mice was also significantly prolonged by FAE.The levels of ROS,MDA,TNF-αand IL-6 decreased,while GSH increased in the FAE treatment group,indicating FAE possesses strong anti-oxidative and anti-inflammatory activity.Western blotting analysis demonstrated that antioxidant proteins Nrf2 and HO-1,tumor suppressors P53 and p-PTEN,and the MAPK pathways in tumor tissues were upregulated by FAE treatment.Serum metabolomics analysis further uncovered that 17 metabolites mostly involving in glycerophospholipid metabolism were correlated with the antitumor effect of FAE.Notably,several lysophosphatidylcholines(LysoPCs)significantly decreased in tumor model group,while FAE treatment restored the changes of these phospholipids to about normal condition.LysoPC acyltransferase 1(LPCAT1)and autotaxin(ATX)highly expressed in melanoma and markedly downregulated by FAE were believed to be responsible for this modulation.CONCLUSION FAE exhibites strong antitumor activity against B16-F10 melanoma through activating MAPKs/Nrf2/HO-1 mediated anti-oxidation and anti-inflammation and modulating glycerophospholipid metabolism via downregulating LPCAT1 and ATX.Besides,it is suggested that serum LysoPCs could be potential biomarkers for the diagnosis and prognosis of melanoma.展开更多
N 1-（2-Fursnidyl）-5-fluorouracil reacted with multimethylenes chlorohydrin in the presence of NaHCO3 in acetonitrile at 80 ℃ to give N 1-（2-furanidyl）-N 3-（hydroxyalkyl）-5-fulorouracils in a high yield （≥93%...N 1-（2-Fursnidyl）-5-fluorouracil reacted with multimethylenes chlorohydrin in the presence of NaHCO3 in acetonitrile at 80 ℃ to give N 1-（2-furanidyl）-N 3-（hydroxyalkyl）-5-fulorouracils in a high yield （≥93%）. Their cyclic glycerophospholipid conjugates were synthesized and had a good activity against the man urinary bladder cancer cell in vitro.展开更多
Milk is a complex biological fluid containing lipids,proteins,carbohydrates and minerals,which are essential for infant growth.While the lipid portion constitutes only 3%-5%of the total milk composition,it accounts fo...Milk is a complex biological fluid containing lipids,proteins,carbohydrates and minerals,which are essential for infant growth.While the lipid portion constitutes only 3%-5%of the total milk composition,it accounts for over 50%of the infant’s daily energy intake.The dominant portion(approximately 98%)is in the form of triacylglycerols and polar lipids,such as glycerophospholipids and sphingolipids,forming minor components.Recently,with the development of lipidomics,important progresses have been made in milk lipidomics,and the identification and quantification of several milk lipids at the group and molecular species level has become a reality,thereby providing useful information for the infant formula industry.In this review,an overview of the separation of the main components of milk lipids was presented,including glycerolipids,phospholipids and sphingolipids.The analytical methods and strategies for milk lipidomics,including gas chromatography-mass spectrometry(MS),capillary electrophoresis MS,nuclear magnetic resonance,matrix-assisted laser desorption ionization-MS,electrospray ionization-MS,shotgun lipidomics and liquid chromatography-MS,were reviewed.Additionally,the bioinformatics of lipidomics for milk lipid determination,including lipid classification,lipid databases and lipid analysis software,were investigated.This review would aid future investigations of the nutrition of milk lipids and refined researches on formula milk powder.展开更多
文摘OBJECTIVE Forsythiae Fructus(Lianqiao)is a typical heat-clearing and detoxicating traditional Chinese medicine(TCM)herb,which has been traditionally used for treating cancer according to TCM theory.However,the underlying mechanism has not been fully explained.METHODS In this study,we investigated the antitumor effect of Forsythiae Fructus aqueous extract(FAE)on B16-F10 melanoma.RESULTS FAE strongly inhibited the tumor growth and metastasis formation in B16-F10 melanoma transplanted mice.The survival time of tumor-bearing mice was also significantly prolonged by FAE.The levels of ROS,MDA,TNF-αand IL-6 decreased,while GSH increased in the FAE treatment group,indicating FAE possesses strong anti-oxidative and anti-inflammatory activity.Western blotting analysis demonstrated that antioxidant proteins Nrf2 and HO-1,tumor suppressors P53 and p-PTEN,and the MAPK pathways in tumor tissues were upregulated by FAE treatment.Serum metabolomics analysis further uncovered that 17 metabolites mostly involving in glycerophospholipid metabolism were correlated with the antitumor effect of FAE.Notably,several lysophosphatidylcholines(LysoPCs)significantly decreased in tumor model group,while FAE treatment restored the changes of these phospholipids to about normal condition.LysoPC acyltransferase 1(LPCAT1)and autotaxin(ATX)highly expressed in melanoma and markedly downregulated by FAE were believed to be responsible for this modulation.CONCLUSION FAE exhibites strong antitumor activity against B16-F10 melanoma through activating MAPKs/Nrf2/HO-1 mediated anti-oxidation and anti-inflammation and modulating glycerophospholipid metabolism via downregulating LPCAT1 and ATX.Besides,it is suggested that serum LysoPCs could be potential biomarkers for the diagnosis and prognosis of melanoma.
文摘N 1-（2-Fursnidyl）-5-fluorouracil reacted with multimethylenes chlorohydrin in the presence of NaHCO3 in acetonitrile at 80 ℃ to give N 1-（2-furanidyl）-N 3-（hydroxyalkyl）-5-fulorouracils in a high yield （≥93%）. Their cyclic glycerophospholipid conjugates were synthesized and had a good activity against the man urinary bladder cancer cell in vitro.
基金Supported by"the 13th Five-Year"National Science and Technology Plan Project of China(2018YFC1603703,2018YFC1604302)the National Natural Science Foundation of China(2013BAD18B03)+3 种基金China Scholarship Council(202008210391)Shenyang Technological Innovation Project(Y17-0-028)Liaoning Revitalization Talents Project(XLYC1902083)Postgraduate Innovation and Cultivation Project of Shenyang Agricultural University(2021YCXB04)。
文摘Milk is a complex biological fluid containing lipids,proteins,carbohydrates and minerals,which are essential for infant growth.While the lipid portion constitutes only 3%-5%of the total milk composition,it accounts for over 50%of the infant’s daily energy intake.The dominant portion(approximately 98%)is in the form of triacylglycerols and polar lipids,such as glycerophospholipids and sphingolipids,forming minor components.Recently,with the development of lipidomics,important progresses have been made in milk lipidomics,and the identification and quantification of several milk lipids at the group and molecular species level has become a reality,thereby providing useful information for the infant formula industry.In this review,an overview of the separation of the main components of milk lipids was presented,including glycerolipids,phospholipids and sphingolipids.The analytical methods and strategies for milk lipidomics,including gas chromatography-mass spectrometry(MS),capillary electrophoresis MS,nuclear magnetic resonance,matrix-assisted laser desorption ionization-MS,electrospray ionization-MS,shotgun lipidomics and liquid chromatography-MS,were reviewed.Additionally,the bioinformatics of lipidomics for milk lipid determination,including lipid classification,lipid databases and lipid analysis software,were investigated.This review would aid future investigations of the nutrition of milk lipids and refined researches on formula milk powder.
