OBJECTIVE To explore the mechanism of action of Qihuang Zhuyu formula(QHZYF)in improving depression after myocardial infarction(MI),with a focus on revealing its regulatory effect on the inflammatory response of the h...OBJECTIVE To explore the mechanism of action of Qihuang Zhuyu formula(QHZYF)in improving depression after myocardial infarction(MI),with a focus on revealing its regulatory effect on the inflammatory response of the heart and brain.METHODS The active ingredients of QHZYF and the action targets for intervening in depression after MI were analyzed by using ultra-performance liquid chromatography-high-resolution mass spectrometry(UPLC-Q-TOF/MS)combined with network pharmacology and molecular docking.A rat model of depression after MI was established by ligation of the left anterior descending coronary artery combined with chronic restraint stress.Echocardiography was used to evaluate cardiac function,hematoxylin-eosin(HE)and Masson staining were used to evaluate myocardial injury,behavioral tests were used to detect melancholic behaviors,Nissl staining was used to evaluate hippocampal neuron injury.Western blot detection of tumor necrosis factor receptor 2(TNFR2),phosphatidylinositol-3-kinase(PI3K),phosphorylated seronine protein kinase(p-AKT),seronine protein kinase(AKT),tumor necrosis factor receptor 1(TNFR1),phosphorylated nuclear factorκB(p-NF-κB),and nuclear factorκB(NF-κB)in cardiac and hippocampal tissues was conducted.The levels of serum IL-6 and IL-10 were detected by enzyme-linked immunosorbent assay(ELISA),and the expression of TNFR1 and TNFR2 was detected by immunohistochemical technique(IHC).In vitro experiments,co-culture of rat cardiomyocyte line H9C2 cells and rat adrenal pheochromocytoma cell line with high differentiation PC12 cells was conducted,TNFR1 inhibitor(H398)and TNFR2 agonist(C-6His)were administered for intervention,and the expression of TNFR2,PI3K,p-AKT,AKT,TNFR1,NF-κB,p-NF-κB was detected by Western blot.Observe the apoptosis of cells by TUNEL staining,ELISA was used to detect the levels of IL-6 and IL-10 in the cell supernatant.RESULTS Network pharmacological analysis indicates that the TNF signaling pathway was a key target for the treatment of depression after MI with the QHZYF.In vivo experiments have confirmed that the intervention of QHZYF could significantly improve the cardiac function,myocardial tissue and hippocampal neuron structure damage of depressed rats after MI,and improve their depression-like behaviors.At the molecular level,the high-dose group of QHZYF significantly upregulated TNFR2,p-AKT/AKT,and IL-10 in cardiac and hippocampal tissues(P<0.01),and downregulated TNFR1,p-NF-κB/NF-κB and IL-6(P<0.01).In vitro experiments showed that the drug-containing serum of QHZYF significantly upregulated the expression of TNFR2,p-AKT/AKT and IL-10 in H9C2 and PC12 cells(P<0.01),downregulated the expression of TNFR1,p-NF-κB/NF-κB and IL-6(P<0.01),and significantly inhibited cell apoptosis(P<0.01).Furthermore,experiments on the combined application of H398 or C-6His further confirmed that its protective and anti-inflammatory effects were mediated by regulating the TNFR2/PI3K/AKT and TNFR1/NF-κB pathways.CONCLUSION QHZYF improves the homeostasis of heart and brain inflammation by regulating the TNF pathway,and ameliorates myocardial injury and depressive state in depressed rats after MI.展开更多
Praising or compliment is a communicative behavior shared by people of different languages. However, due to the influence of unique culture and different society, the language of compliment differs greatly. The author...Praising or compliment is a communicative behavior shared by people of different languages. However, due to the influence of unique culture and different society, the language of compliment differs greatly. The author of this essay is trying to analyze the formula of English compliment shared by all the English speaking nations, esp. in the United States.展开更多
OBJECTIVE Depression is one of the prevalent and prominent complex psychiatric diseases and the number of depressed patients has been on the rise globally during the recent decades.Xiaoyaosan,as a famous Chinese herba...OBJECTIVE Depression is one of the prevalent and prominent complex psychiatric diseases and the number of depressed patients has been on the rise globally during the recent decades.Xiaoyaosan,as a famous Chinese herbal formula,has been widely used in depression patients for a long time. However,the therapeutic mechanisms remain uncertain because of difficulty of depression pathophysiology and the lack of bioinformatic approach to understand the molecular connection. METHODS In this thesis,we applied a network pharmacology approach to explain the potential mechanisms between Xiaoyaosan and depression involved in oral bioavailability screening,drug-likeness assessment,caco-2 permeability,blood-brain barrier target recognition and network analysis. RESULTS 66 active compounds in Xiaoyaosan formula with favorable pharmacokinetic profiles are predicted as active compounds for anti-depression treatment. Network analyses show that these 66 compounds target 40depression-associated proteins including especially HTR2A,NR 3C1,MAOB,XDH and CNR2. These proteins are mainly involved in the neuroactive ligand-receptor interaction,serotonergic synapse,cAMP signaling pathways and calcium signaling pathways. CONCLUSION The integrated network pharmacology method is an effective approach to illustrate the anti-depression mechanisms of herbs,and this in silico approach can be applied in drug discovery.展开更多
A new approach to loading for orthogonal frequency division multiplexing （OFDM） system is proposed, this bit-loading algorithm assigns bits to different subchannels in order to minimize the transmit energy. In the a...A new approach to loading for orthogonal frequency division multiplexing （OFDM） system is proposed, this bit-loading algorithm assigns bits to different subchannels in order to minimize the transmit energy. In the algorithm, first most bit are allocated to each subchannels according to channel condition, Shannon formula and QoS require of the user, then the residual bit are allocated to the subchannels bit by bit. In this way the algorithm is efficient while calculation is less complex. This is the first time to load bits with the scale following Shannon formula and the algorithm is of O （4 N） complexity.展开更多
In this paper, the general calculation formulas of radial matrix elements for relativistic n-dimensional hydrogen atom of spin S=0 are obtained, and the recurrence relation of different power order radial matrix eleme...In this paper, the general calculation formulas of radial matrix elements for relativistic n-dimensional hydrogen atom of spin S=0 are obtained, and the recurrence relation of different power order radial matrix elements are also derived.展开更多
OBJECTIVE Despite the status of cisplatin(DDP) as a classical chemotherapeutic agent in the treatment of cancer,the development of multidrug resistance often leads to a failure of DDP therapy.Traditional Chinese medic...OBJECTIVE Despite the status of cisplatin(DDP) as a classical chemotherapeutic agent in the treatment of cancer,the development of multidrug resistance often leads to a failure of DDP therapy.Traditional Chinese medicine(TCM) as adjuvant chemotherapy of cancer drugs in China has been widely used in cancer treatment.ZuoJin WAN(ZJW),a TCM formula,was proved reversing drug resistance in gastric cancer,but its exact mechanism was still unclear.METHODS CCK-8 assay was used to detect the cell viability.The levels of proteins and mRNA were evaluated using Western blot and q-PCR.Mitochondrial membrane potential was measured by flow cytometry.Depolymerisa.tion of F-actin and translocation of G-actin(gamma-actin) from the cytoplasm to the mitochondria was detected using an immuno fl uorescence assay.RESULTS phosphorylated coflin-1(p-coflin-1) was overexpressed in the DDP-resistant human gastric cancer cell lines SGC7901/DDP and BGC823/DDP,relative to the respective parent cell lines(SGC7901 and BGC823),and DDP induced the dephosphory.lation of p-coflin-1 in both parent lines but not in the DDP-resistant lines.However,ZJW could induce the dephosphorylation of pcoflin-1 and promote coflin-1 translocation from the cytoplasm into the mito.chondria in both SGC7901/DDP and BGC823/DDP cells.This mitochondrial translocation of coflin-1 was found to induce the conversion of flamentous actin to globular-actin,activate mitochondrial dam.age and calcium overloading,and induce the mitochondrial apoptosis pathway.These effects of ZJW on DDP-resistant human gastric cancer cell lines could be reversed via transfection with coflin-1-specifc siRNA,or treatment with a PP1 and PP2A inhibitor.CONCLUSION ZJW can be used as an inhibitor of chemoresistance in gastric cancer,which may partly be due to dephosphorylation of p-coflin-1 via the activation of PP1 and PP2A.展开更多
The determination of material formula needs try-and-error experiment,and consumes large amount of time and fund.In order to solve the problem,a comprehensive method is established,via the experiment of artificial-simi...The determination of material formula needs try-and-error experiment,and consumes large amount of time and fund.In order to solve the problem,a comprehensive method is established,via the experiment of artificial-similar material formula of a mine slope.We controlled the samples by the compactness,and arranged the formula of the test group with the method of the uniform formula experiment.The physical and mechanical parameters of these samples were analyzed using the method of the partial least-squares regression(PLS).And a mathematical model of the indexes of physical and mechanics parameters relating to the factors of formulation constituents was established eventually.We used the model to analyze the effect of each formulation constituent on physical and mechanics parameters of samples.The experiment results and analysis illustrates that1)in the formulation of similar material,the effect of raw materials on the internal friction angleφand cohesion C is opposite;2)The method can highly facilitate the process of the of preparing artificial-similar materials,more economic and effective.展开更多
This paper investigated the natural ventilation driven by hot pressure on a typical office building with single window opening.The actual result indicates that the ventilation rate of natural ventilation driven by hot...This paper investigated the natural ventilation driven by hot pressure on a typical office building with single window opening.The actual result indicates that the ventilation rate of natural ventilation driven by hot pressure in the actual single window opening construction is very different from that obtained by the theoretical formula,since the ventilation conditions between actual environment and ventilation driven by hot pressure in the single window opening are different.Therefore,the theoretical formula is amended,and it becomes more consistent with the actual ventilation rate of natural ventilation driven by hot pressure under conditions that the construction is in the form of single window opening.展开更多
Aim Liver fibrosis is a consequence of chronic liver disease which can progress into liver cirrhosis evenhepatocarcinoma. FuzhengHuayu （FZHY） , a Chinese herbal formula, had been reported to have the effect of anti-...Aim Liver fibrosis is a consequence of chronic liver disease which can progress into liver cirrhosis evenhepatocarcinoma. FuzhengHuayu （FZHY） , a Chinese herbal formula, had been reported to have the effect of anti- fibrosis. This study aims to investigate the effective targets and the mechanisms of FZHY on liver fibrosis. Methods The liver tissue samples of normal group, model group and FZHY-treated group were examined by microarray and iTRAQ. Profiles of differentially expressed genes （DEGs） and differentially expressed proteins （DEPs） in CC14-in- duced liver fibrosis model in rat were analyzed. GO and Pathway were analyzed by DAVID, and protein-protein in- teraction （PPI） was analyzed by STRING and cytoscape. The targets of FZHY compounds were predicted by TCM- SP. Results The results showed that 255 genes （ fold change ≥ 1.5, P 〈 0.05） and 507 proteins （ fold change ≥ 1.2 ,P 〈 0.05 ） were differentially expressed between FZHY group and model group. The high-throughput data of transcriptomics and proteomics was analyzed synthetically. DAVID function annotation analysis showed that these DEGS and DEPs both enriched in 15 GO terms, among drug metabolic process, response to extracellular stimulus, response to vitamin, arachidonic acid metabolic process, response to wounding and oxidation reduction may involve in liver fibrosis. KEGG pathway analysis showed that these DEGS and DEPs both enriched in 9 pathways, among arachidonic acid metabolism, retinol metabolism, metabolism of xenobiotics by cytochrome P450 and drug metabo- lism may be related to liver fibrosis. PPI analysis found that 10 overlapped core genes and proteins, among, Ugt2a3, Cyp2bl and Cyp3al8 were of higher degree, which are all enriched in retinol metabolism. Interestedly, Cyp2bl and Cyp3al8 were also predicted with TCMSP as the targets of FZHY compounds. Conclusion The re- sults indicated that the effective mechanism of FZHY against liver fibrosis is involved in the regulation of multiple targets and multiple pathways, among, retinol metabolism pathway by targeting Ugt2a3, Cyp2bl and Cyp3al8 may play an important role in the treatment of liver fibrosis.展开更多
In this paper we investigate the complexity of several problems concerning 2CNF formulas. At first, we show that the minimal unsatisfiability problem for 2CNF formulas can be solved in linear time. Then we prove that ...In this paper we investigate the complexity of several problems concerning 2CNF formulas. At first, we show that the minimal unsatisfiability problem for 2CNF formulas can be solved in linear time. Then we prove that the problem determining if a 2CNF formula can be transformed to a minimal unsatisfiable formula is also solvable in linear time. Thirdly, we show the polynomial solvability of the satisfiability problem for symmetric monotone formulas in which all clauses has length 2 or ? n - k ( n is the ...展开更多
A new type of iron-based matrix formula as a potential substitute for traditional WC-based matrix formula for hot pressed diamond bit was investigated.Iron,phosphor-iron,663-Cu,nickel,cobalt and certain additives were...A new type of iron-based matrix formula as a potential substitute for traditional WC-based matrix formula for hot pressed diamond bit was investigated.Iron,phosphor-iron,663-Cu,nickel,cobalt and certain additives were selected as the studied formula constituents.Among matrix performances,the hardness and wear resistance were chosen as experimental indexes in this paper.Constrained uniform design method was used for the formula design of iron-based matrix.Two forms of regression models of matrix hardness and wear resistance were obtained by regression analysis using MATLAB.Moreover,the optimization of matrix formulae and matrix performances were also achieved through constrained nonlinear programming.It was found that matrix hardness,significantly affected by the factor of Ni-Co-additives and Fe,increased with the increment of Ni-Co-additives,Fe and P-Fe,but reduced with the increase of 663-Cu.On the other hand,matrix wear resistance is mainly affected by Fe;the effect of the interaction between Fe and P-Fe is also relatively obvious. The increment of 663-Cu powder may result in a slight improvement in matrix wear resistance.In addition,the results of nonlinear programming revealed that the predictive optimum value of hardness was 139.5 HRB and the optimum wear resistance was 0.056 g,whereas they could not reach the optimum value at the same time.展开更多
The frequently used concept of "global reaction" is discussed and the reason for the confusion behind explained. The misconception is cleared by formula writing based on the donor–acceptor(donac) reaction c...The frequently used concept of "global reaction" is discussed and the reason for the confusion behind explained. The misconception is cleared by formula writing based on the donor–acceptor(donac) reaction concept and by applying the Grand Rule of Formula Writing that is based on it.展开更多
OBJECTIVE Bushen Yizhi formula,constructed by Prof LIAO Shi-long′research group(Guangzhou University of chinese medicine),is the combination of clinical experience and modern pharmacological research,and mainly focus...OBJECTIVE Bushen Yizhi formula,constructed by Prof LIAO Shi-long′research group(Guangzhou University of chinese medicine),is the combination of clinical experience and modern pharmacological research,and mainly focuses on the etiology and pathogenesis of AD for treating both cause and symptoms.In this research,the pharmacodynamic study protocol of Bushen Yizhi formula was designed according to the characteristics and indications function of herbs in the formula,and was conformed to preclinical pharmacological research of traditional Chinese medicine for AD,The Technical Requirements of Pharmacology and Toxicology Research in New Drug Development Enacted by State Food and Drug administration also was guiding principle.METHODS A preliminary randomized double-blind clinical trail with 141 cases of AD showed that the efficiency of Bushen Yizhi formula was 61.9%and the increased mean of MMSE was 3.17,but the specific mechanism remains unclear.This study was aimed to reveal the preventive and therapeutic effect of Bushen Yizhi formula on AD rats and its related mechanism.In this research,forebrain-injected IBO-induced AD rats(cholinergic neuron lesion),senescence accelerated mouse,scopolamineinduced learning and memory deficiency rats(mild cognitive impairment)were all established.The learning and memory ability was tested with Morris water maze.Then formation of age pigment,extent of neuronal loss,activation of astrocytes,content of NTFs and degree of oxidized stress damage were determined by morphology,mmunohistochemical and molecular biology methods.RESULTS As the application of Bushen Yizhi formula,the learning and memory ability in all three groups were significantly improved,the formation of age pigment and the content of ACH in cortex and hippocampus were reduced,the activation of astrocytes and release of inflammatory factor(TNF-αand IL-1β)were inhibited,and the antioxidases(CAT,SOD,GSH-PX)were up-regulated and MDA was down-regulated.CONCLUSION Bushen Yizhi formula can prevent and treat AD rats,which might be achieved by anti-inflammatory,antioxidant and protecting cholinergic neuron.展开更多
A new parallel architecture for quantified boolean formula(QBF)solving was proposed,and the prediction model based on machine learning technology was proposed for how sharing knowledge affects the solving performance ...A new parallel architecture for quantified boolean formula(QBF)solving was proposed,and the prediction model based on machine learning technology was proposed for how sharing knowledge affects the solving performance in QBF parallel solving system,and the experimental evaluation scheme was also designed.It shows that the characterization factor of clause and cube influence the solving performance markedly in our experiment.At the same time,the heuristic machine learning algorithm was applied,support vector machine was chosen to predict the performance of QBF parallel solving system based on clause sharing and cube sharing.The relative error of accuracy for prediction can be controlled in a reasonable range of 20%30%.The results show the important and complex role that knowledge sharing plays in any modern parallel solver.It shows that the parallel solver with machine learning reduces the quantity of knowledge sharing about 30%and saving computational resource but does not reduce the performance of solving system.展开更多
A class of parallel implicit Runge-Kutta formulas is constructed for multiprocessor system. A family of parallel implicit two-stage fourth order Runge-Kutta formulas is given. For these formulas, the convergence is pr...A class of parallel implicit Runge-Kutta formulas is constructed for multiprocessor system. A family of parallel implicit two-stage fourth order Runge-Kutta formulas is given. For these formulas, the convergence is proved and the stability analysis is given. The numerical examples demonstrate that these formulas can solve an extensive class of initial value problems for the ordinary differential equations.展开更多
文摘OBJECTIVE To explore the mechanism of action of Qihuang Zhuyu formula(QHZYF)in improving depression after myocardial infarction(MI),with a focus on revealing its regulatory effect on the inflammatory response of the heart and brain.METHODS The active ingredients of QHZYF and the action targets for intervening in depression after MI were analyzed by using ultra-performance liquid chromatography-high-resolution mass spectrometry(UPLC-Q-TOF/MS)combined with network pharmacology and molecular docking.A rat model of depression after MI was established by ligation of the left anterior descending coronary artery combined with chronic restraint stress.Echocardiography was used to evaluate cardiac function,hematoxylin-eosin(HE)and Masson staining were used to evaluate myocardial injury,behavioral tests were used to detect melancholic behaviors,Nissl staining was used to evaluate hippocampal neuron injury.Western blot detection of tumor necrosis factor receptor 2(TNFR2),phosphatidylinositol-3-kinase(PI3K),phosphorylated seronine protein kinase(p-AKT),seronine protein kinase(AKT),tumor necrosis factor receptor 1(TNFR1),phosphorylated nuclear factorκB(p-NF-κB),and nuclear factorκB(NF-κB)in cardiac and hippocampal tissues was conducted.The levels of serum IL-6 and IL-10 were detected by enzyme-linked immunosorbent assay(ELISA),and the expression of TNFR1 and TNFR2 was detected by immunohistochemical technique(IHC).In vitro experiments,co-culture of rat cardiomyocyte line H9C2 cells and rat adrenal pheochromocytoma cell line with high differentiation PC12 cells was conducted,TNFR1 inhibitor(H398)and TNFR2 agonist(C-6His)were administered for intervention,and the expression of TNFR2,PI3K,p-AKT,AKT,TNFR1,NF-κB,p-NF-κB was detected by Western blot.Observe the apoptosis of cells by TUNEL staining,ELISA was used to detect the levels of IL-6 and IL-10 in the cell supernatant.RESULTS Network pharmacological analysis indicates that the TNF signaling pathway was a key target for the treatment of depression after MI with the QHZYF.In vivo experiments have confirmed that the intervention of QHZYF could significantly improve the cardiac function,myocardial tissue and hippocampal neuron structure damage of depressed rats after MI,and improve their depression-like behaviors.At the molecular level,the high-dose group of QHZYF significantly upregulated TNFR2,p-AKT/AKT,and IL-10 in cardiac and hippocampal tissues(P<0.01),and downregulated TNFR1,p-NF-κB/NF-κB and IL-6(P<0.01).In vitro experiments showed that the drug-containing serum of QHZYF significantly upregulated the expression of TNFR2,p-AKT/AKT and IL-10 in H9C2 and PC12 cells(P<0.01),downregulated the expression of TNFR1,p-NF-κB/NF-κB and IL-6(P<0.01),and significantly inhibited cell apoptosis(P<0.01).Furthermore,experiments on the combined application of H398 or C-6His further confirmed that its protective and anti-inflammatory effects were mediated by regulating the TNFR2/PI3K/AKT and TNFR1/NF-κB pathways.CONCLUSION QHZYF improves the homeostasis of heart and brain inflammation by regulating the TNF pathway,and ameliorates myocardial injury and depressive state in depressed rats after MI.
文摘Praising or compliment is a communicative behavior shared by people of different languages. However, due to the influence of unique culture and different society, the language of compliment differs greatly. The author of this essay is trying to analyze the formula of English compliment shared by all the English speaking nations, esp. in the United States.
文摘OBJECTIVE Depression is one of the prevalent and prominent complex psychiatric diseases and the number of depressed patients has been on the rise globally during the recent decades.Xiaoyaosan,as a famous Chinese herbal formula,has been widely used in depression patients for a long time. However,the therapeutic mechanisms remain uncertain because of difficulty of depression pathophysiology and the lack of bioinformatic approach to understand the molecular connection. METHODS In this thesis,we applied a network pharmacology approach to explain the potential mechanisms between Xiaoyaosan and depression involved in oral bioavailability screening,drug-likeness assessment,caco-2 permeability,blood-brain barrier target recognition and network analysis. RESULTS 66 active compounds in Xiaoyaosan formula with favorable pharmacokinetic profiles are predicted as active compounds for anti-depression treatment. Network analyses show that these 66 compounds target 40depression-associated proteins including especially HTR2A,NR 3C1,MAOB,XDH and CNR2. These proteins are mainly involved in the neuroactive ligand-receptor interaction,serotonergic synapse,cAMP signaling pathways and calcium signaling pathways. CONCLUSION The integrated network pharmacology method is an effective approach to illustrate the anti-depression mechanisms of herbs,and this in silico approach can be applied in drug discovery.
文摘A new approach to loading for orthogonal frequency division multiplexing （OFDM） system is proposed, this bit-loading algorithm assigns bits to different subchannels in order to minimize the transmit energy. In the algorithm, first most bit are allocated to each subchannels according to channel condition, Shannon formula and QoS require of the user, then the residual bit are allocated to the subchannels bit by bit. In this way the algorithm is efficient while calculation is less complex. This is the first time to load bits with the scale following Shannon formula and the algorithm is of O （4 N） complexity.
文摘In this paper, the general calculation formulas of radial matrix elements for relativistic n-dimensional hydrogen atom of spin S=0 are obtained, and the recurrence relation of different power order radial matrix elements are also derived.
基金supported by National Natural Science Foundation of China(81473481)Construct Program of the Key Discipline of State Administration of Traditional Chinese Medicine of People′s Republic of Chinathe Science Foundation for Shanghai Municipal Health and Family Planning Commission(20124065)
文摘OBJECTIVE Despite the status of cisplatin(DDP) as a classical chemotherapeutic agent in the treatment of cancer,the development of multidrug resistance often leads to a failure of DDP therapy.Traditional Chinese medicine(TCM) as adjuvant chemotherapy of cancer drugs in China has been widely used in cancer treatment.ZuoJin WAN(ZJW),a TCM formula,was proved reversing drug resistance in gastric cancer,but its exact mechanism was still unclear.METHODS CCK-8 assay was used to detect the cell viability.The levels of proteins and mRNA were evaluated using Western blot and q-PCR.Mitochondrial membrane potential was measured by flow cytometry.Depolymerisa.tion of F-actin and translocation of G-actin(gamma-actin) from the cytoplasm to the mitochondria was detected using an immuno fl uorescence assay.RESULTS phosphorylated coflin-1(p-coflin-1) was overexpressed in the DDP-resistant human gastric cancer cell lines SGC7901/DDP and BGC823/DDP,relative to the respective parent cell lines(SGC7901 and BGC823),and DDP induced the dephosphory.lation of p-coflin-1 in both parent lines but not in the DDP-resistant lines.However,ZJW could induce the dephosphorylation of pcoflin-1 and promote coflin-1 translocation from the cytoplasm into the mito.chondria in both SGC7901/DDP and BGC823/DDP cells.This mitochondrial translocation of coflin-1 was found to induce the conversion of flamentous actin to globular-actin,activate mitochondrial dam.age and calcium overloading,and induce the mitochondrial apoptosis pathway.These effects of ZJW on DDP-resistant human gastric cancer cell lines could be reversed via transfection with coflin-1-specifc siRNA,or treatment with a PP1 and PP2A inhibitor.CONCLUSION ZJW can be used as an inhibitor of chemoresistance in gastric cancer,which may partly be due to dephosphorylation of p-coflin-1 via the activation of PP1 and PP2A.
基金Projects(41372312,51379194)supported by the National Natural Science Foundation of ChinaProject(CUGL140817)supported by the Fundamental Research Funds for the Central Universities of China University of Geosciences(Wuhan)+1 种基金Project(2014CFB894)supported by the Natural Science Foundation of Hubei Province of ChinaProject(2014M552113)supported by the China Postdoctoral Science Foundation
文摘The determination of material formula needs try-and-error experiment,and consumes large amount of time and fund.In order to solve the problem,a comprehensive method is established,via the experiment of artificial-similar material formula of a mine slope.We controlled the samples by the compactness,and arranged the formula of the test group with the method of the uniform formula experiment.The physical and mechanical parameters of these samples were analyzed using the method of the partial least-squares regression(PLS).And a mathematical model of the indexes of physical and mechanics parameters relating to the factors of formulation constituents was established eventually.We used the model to analyze the effect of each formulation constituent on physical and mechanics parameters of samples.The experiment results and analysis illustrates that1)in the formulation of similar material,the effect of raw materials on the internal friction angleφand cohesion C is opposite;2)The method can highly facilitate the process of the of preparing artificial-similar materials,more economic and effective.
基金Supported by "Eleventh Five-Year" National Scientific and Technological Support Scheme Funded Projects(2006BAJ02A02,2006BAJ02A05)Ministry of Education,the New Century Talents Scheme Funded Projects(Education and Technology Divi-sion[2007]No.209)National Personnel Merit-Based Projects(Office of Hair People[2007]170)
文摘This paper investigated the natural ventilation driven by hot pressure on a typical office building with single window opening.The actual result indicates that the ventilation rate of natural ventilation driven by hot pressure in the actual single window opening construction is very different from that obtained by the theoretical formula,since the ventilation conditions between actual environment and ventilation driven by hot pressure in the single window opening are different.Therefore,the theoretical formula is amended,and it becomes more consistent with the actual ventilation rate of natural ventilation driven by hot pressure under conditions that the construction is in the form of single window opening.
文摘Aim Liver fibrosis is a consequence of chronic liver disease which can progress into liver cirrhosis evenhepatocarcinoma. FuzhengHuayu （FZHY） , a Chinese herbal formula, had been reported to have the effect of anti- fibrosis. This study aims to investigate the effective targets and the mechanisms of FZHY on liver fibrosis. Methods The liver tissue samples of normal group, model group and FZHY-treated group were examined by microarray and iTRAQ. Profiles of differentially expressed genes （DEGs） and differentially expressed proteins （DEPs） in CC14-in- duced liver fibrosis model in rat were analyzed. GO and Pathway were analyzed by DAVID, and protein-protein in- teraction （PPI） was analyzed by STRING and cytoscape. The targets of FZHY compounds were predicted by TCM- SP. Results The results showed that 255 genes （ fold change ≥ 1.5, P 〈 0.05） and 507 proteins （ fold change ≥ 1.2 ,P 〈 0.05 ） were differentially expressed between FZHY group and model group. The high-throughput data of transcriptomics and proteomics was analyzed synthetically. DAVID function annotation analysis showed that these DEGS and DEPs both enriched in 15 GO terms, among drug metabolic process, response to extracellular stimulus, response to vitamin, arachidonic acid metabolic process, response to wounding and oxidation reduction may involve in liver fibrosis. KEGG pathway analysis showed that these DEGS and DEPs both enriched in 9 pathways, among arachidonic acid metabolism, retinol metabolism, metabolism of xenobiotics by cytochrome P450 and drug metabo- lism may be related to liver fibrosis. PPI analysis found that 10 overlapped core genes and proteins, among, Ugt2a3, Cyp2bl and Cyp3al8 were of higher degree, which are all enriched in retinol metabolism. Interestedly, Cyp2bl and Cyp3al8 were also predicted with TCMSP as the targets of FZHY compounds. Conclusion The re- sults indicated that the effective mechanism of FZHY against liver fibrosis is involved in the regulation of multiple targets and multiple pathways, among, retinol metabolism pathway by targeting Ugt2a3, Cyp2bl and Cyp3al8 may play an important role in the treatment of liver fibrosis.
文摘In this paper we investigate the complexity of several problems concerning 2CNF formulas. At first, we show that the minimal unsatisfiability problem for 2CNF formulas can be solved in linear time. Then we prove that the problem determining if a 2CNF formula can be transformed to a minimal unsatisfiable formula is also solvable in linear time. Thirdly, we show the polynomial solvability of the satisfiability problem for symmetric monotone formulas in which all clauses has length 2 or ? n - k ( n is the ...
文摘A new type of iron-based matrix formula as a potential substitute for traditional WC-based matrix formula for hot pressed diamond bit was investigated.Iron,phosphor-iron,663-Cu,nickel,cobalt and certain additives were selected as the studied formula constituents.Among matrix performances,the hardness and wear resistance were chosen as experimental indexes in this paper.Constrained uniform design method was used for the formula design of iron-based matrix.Two forms of regression models of matrix hardness and wear resistance were obtained by regression analysis using MATLAB.Moreover,the optimization of matrix formulae and matrix performances were also achieved through constrained nonlinear programming.It was found that matrix hardness,significantly affected by the factor of Ni-Co-additives and Fe,increased with the increment of Ni-Co-additives,Fe and P-Fe,but reduced with the increase of 663-Cu.On the other hand,matrix wear resistance is mainly affected by Fe;the effect of the interaction between Fe and P-Fe is also relatively obvious. The increment of 663-Cu powder may result in a slight improvement in matrix wear resistance.In addition,the results of nonlinear programming revealed that the predictive optimum value of hardness was 139.5 HRB and the optimum wear resistance was 0.056 g,whereas they could not reach the optimum value at the same time.
文摘The frequently used concept of "global reaction" is discussed and the reason for the confusion behind explained. The misconception is cleared by formula writing based on the donor–acceptor(donac) reaction concept and by applying the Grand Rule of Formula Writing that is based on it.
基金The project supported by National Natural Science Foundation of China(81273817)Shandong Province Natural Science Foundation of China(ZR2013HL062)by Foundation of Overseas Distinguished Taishan Scholars of Shandong Province
文摘OBJECTIVE Bushen Yizhi formula,constructed by Prof LIAO Shi-long′research group(Guangzhou University of chinese medicine),is the combination of clinical experience and modern pharmacological research,and mainly focuses on the etiology and pathogenesis of AD for treating both cause and symptoms.In this research,the pharmacodynamic study protocol of Bushen Yizhi formula was designed according to the characteristics and indications function of herbs in the formula,and was conformed to preclinical pharmacological research of traditional Chinese medicine for AD,The Technical Requirements of Pharmacology and Toxicology Research in New Drug Development Enacted by State Food and Drug administration also was guiding principle.METHODS A preliminary randomized double-blind clinical trail with 141 cases of AD showed that the efficiency of Bushen Yizhi formula was 61.9%and the increased mean of MMSE was 3.17,but the specific mechanism remains unclear.This study was aimed to reveal the preventive and therapeutic effect of Bushen Yizhi formula on AD rats and its related mechanism.In this research,forebrain-injected IBO-induced AD rats(cholinergic neuron lesion),senescence accelerated mouse,scopolamineinduced learning and memory deficiency rats(mild cognitive impairment)were all established.The learning and memory ability was tested with Morris water maze.Then formation of age pigment,extent of neuronal loss,activation of astrocytes,content of NTFs and degree of oxidized stress damage were determined by morphology,mmunohistochemical and molecular biology methods.RESULTS As the application of Bushen Yizhi formula,the learning and memory ability in all three groups were significantly improved,the formation of age pigment and the content of ACH in cortex and hippocampus were reduced,the activation of astrocytes and release of inflammatory factor(TNF-αand IL-1β)were inhibited,and the antioxidases(CAT,SOD,GSH-PX)were up-regulated and MDA was down-regulated.CONCLUSION Bushen Yizhi formula can prevent and treat AD rats,which might be achieved by anti-inflammatory,antioxidant and protecting cholinergic neuron.
基金Project(61171141)supported by the National Natural Science Foundation of China
文摘A new parallel architecture for quantified boolean formula(QBF)solving was proposed,and the prediction model based on machine learning technology was proposed for how sharing knowledge affects the solving performance in QBF parallel solving system,and the experimental evaluation scheme was also designed.It shows that the characterization factor of clause and cube influence the solving performance markedly in our experiment.At the same time,the heuristic machine learning algorithm was applied,support vector machine was chosen to predict the performance of QBF parallel solving system based on clause sharing and cube sharing.The relative error of accuracy for prediction can be controlled in a reasonable range of 20%30%.The results show the important and complex role that knowledge sharing plays in any modern parallel solver.It shows that the parallel solver with machine learning reduces the quantity of knowledge sharing about 30%and saving computational resource but does not reduce the performance of solving system.
基金Project supported by the National Natural Science Foundation of China
文摘A class of parallel implicit Runge-Kutta formulas is constructed for multiprocessor system. A family of parallel implicit two-stage fourth order Runge-Kutta formulas is given. For these formulas, the convergence is proved and the stability analysis is given. The numerical examples demonstrate that these formulas can solve an extensive class of initial value problems for the ordinary differential equations.
