Exosomes,ubiquitously present in body fluids,serve as non-invasive biomarkers for disease diagnosis,monitoring,and treatment.As intercellular messengers,exosomes encapsulate a rich array of proteins,nucleic acids,and ...Exosomes,ubiquitously present in body fluids,serve as non-invasive biomarkers for disease diagnosis,monitoring,and treatment.As intercellular messengers,exosomes encapsulate a rich array of proteins,nucleic acids,and metabolites,although most studies have primarily focused on proteins and RNA.Recently,exosome metabolomics has demonstrated clinical value and potential advantages in disease detection and pathophysiology,despite significant challenges,particularly in exosome isolation and metabolite detection.This review discusses the significant technical challenges in exosome isolation and metabolite detection,highlighting the advancements in these areas that support the clinical application of exosome metabolomics,and illustrates the potential of exosomal metabolites from various body fluids as biomarkers for early disease diagnosis and treatment.展开更多
目的 探讨自发性蝶窦外侧隐窝脑脊液鼻漏的临床特点、诊断及手术治疗。方法与结果 回顾分析2019年1月至2023年6月首都医科大学附属北京同仁医院收治的24例自发性蝶窦外侧隐窝脑脊液鼻漏患者的临床资料。男性7例、女性17例,平均年龄为46...目的 探讨自发性蝶窦外侧隐窝脑脊液鼻漏的临床特点、诊断及手术治疗。方法与结果 回顾分析2019年1月至2023年6月首都医科大学附属北京同仁医院收治的24例自发性蝶窦外侧隐窝脑脊液鼻漏患者的临床资料。男性7例、女性17例,平均年龄为46岁,平均体重指数为27.24 kg/m^(2);21例术前腰椎穿刺脑脊液压力平均为200 mm H_(2)O,其中11例≥200 mm H_(2)O;影像学检查均存在骨质缺损以及脑膜脑膨出疝入蝶窦内。所有患者均行经鼻内镜脑脊液鼻漏修补术,其中2例术前脑脊液压力≥300 mm H_(2)O,先行腰大池-腹腔分流术;余22例先行内镜下经翼突入路蝶窦外侧隐窝脑膜脑膨出切除术伴颅底漏口修补术。术后2周21例复查脑脊液压力为140~320 mm H_(2)O,平均185 mm H_(2)O;平均随访25.40个月,3例脑脊液鼻漏复发,行腰大池-腹腔分流术,最终所有患者均实现临床治愈。结论 自发性脑脊液鼻漏多与慢性颅内压增高有关,经鼻内镜漏口修补及分流手术是治愈并防止复发的有效方法。展开更多
文摘Exosomes,ubiquitously present in body fluids,serve as non-invasive biomarkers for disease diagnosis,monitoring,and treatment.As intercellular messengers,exosomes encapsulate a rich array of proteins,nucleic acids,and metabolites,although most studies have primarily focused on proteins and RNA.Recently,exosome metabolomics has demonstrated clinical value and potential advantages in disease detection and pathophysiology,despite significant challenges,particularly in exosome isolation and metabolite detection.This review discusses the significant technical challenges in exosome isolation and metabolite detection,highlighting the advancements in these areas that support the clinical application of exosome metabolomics,and illustrates the potential of exosomal metabolites from various body fluids as biomarkers for early disease diagnosis and treatment.
文摘目的 探讨自发性蝶窦外侧隐窝脑脊液鼻漏的临床特点、诊断及手术治疗。方法与结果 回顾分析2019年1月至2023年6月首都医科大学附属北京同仁医院收治的24例自发性蝶窦外侧隐窝脑脊液鼻漏患者的临床资料。男性7例、女性17例,平均年龄为46岁,平均体重指数为27.24 kg/m^(2);21例术前腰椎穿刺脑脊液压力平均为200 mm H_(2)O,其中11例≥200 mm H_(2)O;影像学检查均存在骨质缺损以及脑膜脑膨出疝入蝶窦内。所有患者均行经鼻内镜脑脊液鼻漏修补术,其中2例术前脑脊液压力≥300 mm H_(2)O,先行腰大池-腹腔分流术;余22例先行内镜下经翼突入路蝶窦外侧隐窝脑膜脑膨出切除术伴颅底漏口修补术。术后2周21例复查脑脊液压力为140~320 mm H_(2)O,平均185 mm H_(2)O;平均随访25.40个月,3例脑脊液鼻漏复发,行腰大池-腹腔分流术,最终所有患者均实现临床治愈。结论 自发性脑脊液鼻漏多与慢性颅内压增高有关,经鼻内镜漏口修补及分流手术是治愈并防止复发的有效方法。
