In this study,the structural characters,antioxidant activities and bile acid-binding ability of sea buckthorn polysaccharides(HRPs)obtained by the commonly used hot water(HRP-W),pressurized hot water(HRP-H),ultrasonic...In this study,the structural characters,antioxidant activities and bile acid-binding ability of sea buckthorn polysaccharides(HRPs)obtained by the commonly used hot water(HRP-W),pressurized hot water(HRP-H),ultrasonic(HRP-U),acid(HRP-C)and alkali(HRP-A)assisted extraction methods were investigated.The results demonstrated that extraction methods had significant effects on extraction yield,monosaccharide composition,molecular weight,particle size,triple-helical structure,and surface morphology of HRPs except for the major linkage bands.Thermogravimetric analysis showed that HRP-U with filamentous reticular microstructure exhibited better thermal stability.The HRP-A with the lowest molecular weight and highest arabinose content possessed the best antioxidant activities.Moreover,the rheological analysis indicated that HRPs with higher galacturonic acid content and molecular weight showed higher viscosity and stronger crosslinking network(HRP-C,HRP-W and HRP-U),which exhibited stronger bile acid binding capacity.The present findings provide scientific evidence in the preparation technology of sea buckthorn polysaccharides with good antioxidant and bile acid binding capacity which are related to the structure affected by the extraction methods.展开更多
This article introduces the concept of load aggregation,which involves a comprehensive analysis of loads to acquire their external characteristics for the purpose of modeling and analyzing power systems.The online ide...This article introduces the concept of load aggregation,which involves a comprehensive analysis of loads to acquire their external characteristics for the purpose of modeling and analyzing power systems.The online identification method is a computer-involved approach for data collection,processing,and system identification,commonly used for adaptive control and prediction.This paper proposes a method for dynamically aggregating large-scale adjustable loads to support high proportions of new energy integration,aiming to study the aggregation characteristics of regional large-scale adjustable loads using online identification techniques and feature extraction methods.The experiment selected 300 central air conditioners as the research subject and analyzed their regulation characteristics,economic efficiency,and comfort.The experimental results show that as the adjustment time of the air conditioner increases from 5 minutes to 35 minutes,the stable adjustment quantity during the adjustment period decreases from 28.46 to 3.57,indicating that air conditioning loads can be controlled over a long period and have better adjustment effects in the short term.Overall,the experimental results of this paper demonstrate that analyzing the aggregation characteristics of regional large-scale adjustable loads using online identification techniques and feature extraction algorithms is effective.展开更多
Polysaccharides are the important biologically active components found in the peel of Dioscorea opposita Thunb.(DTTP).The influences of 4 extraction methods,namely hot water extraction(W),acidic extraction(HA),hot-com...Polysaccharides are the important biologically active components found in the peel of Dioscorea opposita Thunb.(DTTP).The influences of 4 extraction methods,namely hot water extraction(W),acidic extraction(HA),hot-compressed water extraction(HCW)and enzyme-assisted extraction(EAE),on the yields,physicochemical properties,hypoglycemic and antioxidant activities of polysaccharides from DTTP were studied and compared.Among these DTTP polysaccharides,DTTP-HA was outstanding in several respects.DTTP-HA was the most water soluble;it had the highest total carbohydrate content(85.08%),the highest uronic acid(13.20%)and the highest thermal stability.DTTP-HA and DTTP-W possessed a triple-helix structure.These 4 kinds of polysaccharides have the same types of monosaccharides,but in different molar percentages.Extraction method had a significant impact on the microstructures of the extracted polysaccharides.DTT-HA exhibited irregular structure with many holes.Among the 4 extracted methods,the DTTP-HA and DTTP-W initially exhibited higher hypoglycemic and antioxidant activities.The better bioactivities of DTTP-HA may be related to the above factors.The findings indicated that acid extraction is an effective method to extract polysaccharides with high biological activities from DTTP.展开更多
As naturally sourced proteins,peanut proteins have garnered significant attention from the food industry,owing to their numerous advantages,such as easy extraction,non-pungency,and high bioavailability.Furthermore,pea...As naturally sourced proteins,peanut proteins have garnered significant attention from the food industry,owing to their numerous advantages,such as easy extraction,non-pungency,and high bioavailability.Furthermore,peanut proteins are highly digestible in the gastrointestinal tract and boast a high net protein utilization rate,making them an appealing protein source in food products and a promising alternative to animal protein.In this paper,the recent works on the extraction method,modification method,and application of peanut proteins were reviewed.Both advantages and disadvantages of current extraction and modification were discussed.Recently updated information about peanut protein research was summarized.Based on these,the prospection of peanut proteins research was presented,which may be instructive for future research in this field.Future research is still needed for accessible modification methods to develop the functional properties of peanut proteins.展开更多
The basic method of DNA extraction （CTAB） was improved as the multi-times STE-CTAB extraction method and used to extract the DNA of birch leaved in this experiment. Results showed that the improved method is suitabl...The basic method of DNA extraction （CTAB） was improved as the multi-times STE-CTAB extraction method and used to extract the DNA of birch leaved in this experiment. Results showed that the improved method is suitable not only for genomic DNA extraction of birch but also for that of other plants. The purity of genornic DNA extracted by the.multi-times STE-CTAB extraction method is higher than that by one time STE-CTAB method, and it does not need the process of RNase. The factors of influencing ISSR system were explored based on the genomic DNA of birch extracted by the two methods. The optimal conditions for ISSR system were determined as follows： Mg2＋ concentration is 1.5-3.0 mmol·L^-1, dNTP concentration 0.104）.25 mmol·L^-1, the quantity of Taq polymerase 0.5-2.0 U, template DNA 30-100 ng, and the concentration of primer is 0.2-0.4 pmmol·L^-1, and the reaction program was as： initial denaturation for 5 min at 94℃, 30 cycles of denaturation for 30 s at 94℃,annealing for 30 s at 51℃, extension for 30 s at 72℃, and a final 7 min extension at 72℃.展开更多
We compare three different methods to extract coherent modes from Doppler backscattering （DBS）, which are center of gravity （COG） of the complex amplitude spectrum, spectrum of DBS phase derivative （phase derivat...We compare three different methods to extract coherent modes from Doppler backscattering （DBS）, which are center of gravity （COG） of the complex amplitude spectrum, spectrum of DBS phase derivative （phase derivative method）, and phase spectrum, respectively. These three methods are all feasible to extract coherent modes, for example, geodesic acoustic mode oscillation. However, there are stilI differences between dealing with high frequency modes （several hundred kHz） and low frequency modes, （several kHz） hiding in DBS signal. There is a significant amount of power at low frequencies in the phase spectrum, which can be removed by using the phase derivative method and COG. High frequency modes are clearer by using the COO and the phase derivative method than the phase spectrum. The spectrum of DBS amplitude does not show the coherent modes detected by using COG, phase derivative method and phase spectrum. When two Doppler shifted peaks exist, coherent modes and their harmonics appear in the spectrum of DBS amplitude, which are introduced by the DBS phase.展开更多
The determination method of 10-hydroxycamptothecin in Camptotheca acuminata fruits by high-performance liquid chromatogram (HPLC) was studied. The HPLC analysis was performed on a HIQ sil C18(4.6×250 mm) column w...The determination method of 10-hydroxycamptothecin in Camptotheca acuminata fruits by high-performance liquid chromatogram (HPLC) was studied. The HPLC analysis was performed on a HIQ sil C18(4.6×250 mm) column with mobile phase of acetonitrilewater (3:7, V:V), flow rate 1 mLmin-1 and UV detective wavelength 266 nm. Extracting 10-hydroxycamptothecin by ultrasonic method from fruits of C. acuminata to prepare samples for analysis was systematically discussed. The optimal extraction condition was carried out by 60% alcohol solution at 60℃ for 50 minutes.展开更多
The cellulase was used for extracting the volatile constituents of the Leonurus japonicus Houtt.The effects of the pH,the amounts and the fineness of cellulase were discussed.The average yield obtained was 4.05%.16 ch...The cellulase was used for extracting the volatile constituents of the Leonurus japonicus Houtt.The effects of the pH,the amounts and the fineness of cellulase were discussed.The average yield obtained was 4.05%.16 chemical components were identified by gas chromatography-mass spectrometry (GC-MS)method.The cellulase extraction (CE)method was made a camparison with distillation extraction (DSE)method.展开更多
Purpose： This study aims to build an automatic survey generation tool, named CitationAS, based on citation content as represented by the set of citing sentences in the original articles.Design/methodology/approach： ...Purpose： This study aims to build an automatic survey generation tool, named CitationAS, based on citation content as represented by the set of citing sentences in the original articles.Design/methodology/approach： Firstly, we apply LDA to analyse topic distribution of citation content. Secondly, in CitationAS, we use bisecting K-means, Lingo and STC to cluster retrieved citation content. Then Word2Vec, Word Net and combination of them are applied to generate cluster labels. Next, we employ TF-IDF, MMR, as well as considering sentence location information, to extract important sentences, which are used to generate surveys. Finally, we adopt manual evaluation for the generated surveys.Findings： In experiments, we choose 20 high-frequency phrases as search terms. Results show that Lingo-Word2Vec, STC-Word Net and bisecting K-means-Word2Vec have better clustering effects. In 5 points evaluation system, survey quality scores obtained by designing methods are close to 3, indicating surveys are within acceptable limits. When considering sentence location information, survey quality will be improved. Combination of Lingo, Word2Vec, TF-IDF or MMR can acquire higher survey quality.Research limitations： The manual evaluation method may have a certain subjectivity. We use a simple linear function to combine Word2Vec and Word Net that may not bring out their strengths. The generated surveys may not contain some newly created knowledge of some articles which may concentrate on sentences with no citing.Practical implications： CitationAS tool can automatically generate a comprehensive, detailed and accurate survey according to user’s search terms. It can also help researchers learn about research status in a certain field.Originality/value： Citaiton AS tool is of practicability. It merges cluster labels from semantic level to improve clustering results. The tool also considers sentence location information when calculating sentence score by TF-IDF and MMR.展开更多
基金The Guangdong Basic and Applied Basic Research Foundation(2022A1515010730)National Natural Science Foundation of China(32001647)+2 种基金National Natural Science Foundation of China(31972022)Financial and moral assistance supported by the Guangdong Basic and Applied Basic Research Foundation(2019A1515011996)111 Project(B17018)。
文摘In this study,the structural characters,antioxidant activities and bile acid-binding ability of sea buckthorn polysaccharides(HRPs)obtained by the commonly used hot water(HRP-W),pressurized hot water(HRP-H),ultrasonic(HRP-U),acid(HRP-C)and alkali(HRP-A)assisted extraction methods were investigated.The results demonstrated that extraction methods had significant effects on extraction yield,monosaccharide composition,molecular weight,particle size,triple-helical structure,and surface morphology of HRPs except for the major linkage bands.Thermogravimetric analysis showed that HRP-U with filamentous reticular microstructure exhibited better thermal stability.The HRP-A with the lowest molecular weight and highest arabinose content possessed the best antioxidant activities.Moreover,the rheological analysis indicated that HRPs with higher galacturonic acid content and molecular weight showed higher viscosity and stronger crosslinking network(HRP-C,HRP-W and HRP-U),which exhibited stronger bile acid binding capacity.The present findings provide scientific evidence in the preparation technology of sea buckthorn polysaccharides with good antioxidant and bile acid binding capacity which are related to the structure affected by the extraction methods.
基金supported by the State Grid Science&Technology Project(5100-202114296A-0-0-00).
文摘This article introduces the concept of load aggregation,which involves a comprehensive analysis of loads to acquire their external characteristics for the purpose of modeling and analyzing power systems.The online identification method is a computer-involved approach for data collection,processing,and system identification,commonly used for adaptive control and prediction.This paper proposes a method for dynamically aggregating large-scale adjustable loads to support high proportions of new energy integration,aiming to study the aggregation characteristics of regional large-scale adjustable loads using online identification techniques and feature extraction methods.The experiment selected 300 central air conditioners as the research subject and analyzed their regulation characteristics,economic efficiency,and comfort.The experimental results show that as the adjustment time of the air conditioner increases from 5 minutes to 35 minutes,the stable adjustment quantity during the adjustment period decreases from 28.46 to 3.57,indicating that air conditioning loads can be controlled over a long period and have better adjustment effects in the short term.Overall,the experimental results of this paper demonstrate that analyzing the aggregation characteristics of regional large-scale adjustable loads using online identification techniques and feature extraction algorithms is effective.
基金the financial support provided by Zhengzhou 1125 Talents Gathering Project (2018)the Doctor startup fund of Henan Agricultural University (30500431)
文摘Polysaccharides are the important biologically active components found in the peel of Dioscorea opposita Thunb.(DTTP).The influences of 4 extraction methods,namely hot water extraction(W),acidic extraction(HA),hot-compressed water extraction(HCW)and enzyme-assisted extraction(EAE),on the yields,physicochemical properties,hypoglycemic and antioxidant activities of polysaccharides from DTTP were studied and compared.Among these DTTP polysaccharides,DTTP-HA was outstanding in several respects.DTTP-HA was the most water soluble;it had the highest total carbohydrate content(85.08%),the highest uronic acid(13.20%)and the highest thermal stability.DTTP-HA and DTTP-W possessed a triple-helix structure.These 4 kinds of polysaccharides have the same types of monosaccharides,but in different molar percentages.Extraction method had a significant impact on the microstructures of the extracted polysaccharides.DTT-HA exhibited irregular structure with many holes.Among the 4 extracted methods,the DTTP-HA and DTTP-W initially exhibited higher hypoglycemic and antioxidant activities.The better bioactivities of DTTP-HA may be related to the above factors.The findings indicated that acid extraction is an effective method to extract polysaccharides with high biological activities from DTTP.
基金the Natural Science Foundation of Shandong Province[grant number ZR2020QC218]Key R&D plan of Shandong Province[grant number 2019YYSP005]+2 种基金Major Science and Technology Projects of Shandong Province[grant number2019JZZY010722]Qingdao Municipal Science and Technology Benefit People Project[grant number 20-3-4-34-nsh]Breeding Plan of Shandong Provincial Qingchuang Research Team[grant number 2021-Innovation Team of Functional Plant Protein-Based Food]。
文摘As naturally sourced proteins,peanut proteins have garnered significant attention from the food industry,owing to their numerous advantages,such as easy extraction,non-pungency,and high bioavailability.Furthermore,peanut proteins are highly digestible in the gastrointestinal tract and boast a high net protein utilization rate,making them an appealing protein source in food products and a promising alternative to animal protein.In this paper,the recent works on the extraction method,modification method,and application of peanut proteins were reviewed.Both advantages and disadvantages of current extraction and modification were discussed.Recently updated information about peanut protein research was summarized.Based on these,the prospection of peanut proteins research was presented,which may be instructive for future research in this field.Future research is still needed for accessible modification methods to develop the functional properties of peanut proteins.
基金This paper was supported by National Natural Science Foundation of China (No. 30571513) and National High Technology Research and Development Program of China (863 Program) (No. 2002AA241080).
文摘The basic method of DNA extraction （CTAB） was improved as the multi-times STE-CTAB extraction method and used to extract the DNA of birch leaved in this experiment. Results showed that the improved method is suitable not only for genomic DNA extraction of birch but also for that of other plants. The purity of genornic DNA extracted by the.multi-times STE-CTAB extraction method is higher than that by one time STE-CTAB method, and it does not need the process of RNase. The factors of influencing ISSR system were explored based on the genomic DNA of birch extracted by the two methods. The optimal conditions for ISSR system were determined as follows： Mg2＋ concentration is 1.5-3.0 mmol·L^-1, dNTP concentration 0.104）.25 mmol·L^-1, the quantity of Taq polymerase 0.5-2.0 U, template DNA 30-100 ng, and the concentration of primer is 0.2-0.4 pmmol·L^-1, and the reaction program was as： initial denaturation for 5 min at 94℃, 30 cycles of denaturation for 30 s at 94℃,annealing for 30 s at 51℃, extension for 30 s at 72℃, and a final 7 min extension at 72℃.
基金Supported by the National Natural Science Foundation of China under Grant Nos 10990210 and 11475173the National Magnetic Confinement Fusion Energy Program of China under Grant Nos 2013GB106002 and 2014GB109002
文摘We compare three different methods to extract coherent modes from Doppler backscattering （DBS）, which are center of gravity （COG） of the complex amplitude spectrum, spectrum of DBS phase derivative （phase derivative method）, and phase spectrum, respectively. These three methods are all feasible to extract coherent modes, for example, geodesic acoustic mode oscillation. However, there are stilI differences between dealing with high frequency modes （several hundred kHz） and low frequency modes, （several kHz） hiding in DBS signal. There is a significant amount of power at low frequencies in the phase spectrum, which can be removed by using the phase derivative method and COG. High frequency modes are clearer by using the COO and the phase derivative method than the phase spectrum. The spectrum of DBS amplitude does not show the coherent modes detected by using COG, phase derivative method and phase spectrum. When two Doppler shifted peaks exist, coherent modes and their harmonics appear in the spectrum of DBS amplitude, which are introduced by the DBS phase.
基金This paper was supported by National Natural Science Foundation of China (No. 30070086).
文摘The determination method of 10-hydroxycamptothecin in Camptotheca acuminata fruits by high-performance liquid chromatogram (HPLC) was studied. The HPLC analysis was performed on a HIQ sil C18(4.6×250 mm) column with mobile phase of acetonitrilewater (3:7, V:V), flow rate 1 mLmin-1 and UV detective wavelength 266 nm. Extracting 10-hydroxycamptothecin by ultrasonic method from fruits of C. acuminata to prepare samples for analysis was systematically discussed. The optimal extraction condition was carried out by 60% alcohol solution at 60℃ for 50 minutes.
文摘The cellulase was used for extracting the volatile constituents of the Leonurus japonicus Houtt.The effects of the pH,the amounts and the fineness of cellulase were discussed.The average yield obtained was 4.05%.16 chemical components were identified by gas chromatography-mass spectrometry (GC-MS)method.The cellulase extraction (CE)method was made a camparison with distillation extraction (DSE)method.
基金supported by Major Projects of National Social Science Fund (No. 17ZDA291)Fujian Provincial Key Laboratory of Information Processing and Intelligent Control (Minjiang University) (No. MJUKF201704)Qing Lan Project
文摘Purpose： This study aims to build an automatic survey generation tool, named CitationAS, based on citation content as represented by the set of citing sentences in the original articles.Design/methodology/approach： Firstly, we apply LDA to analyse topic distribution of citation content. Secondly, in CitationAS, we use bisecting K-means, Lingo and STC to cluster retrieved citation content. Then Word2Vec, Word Net and combination of them are applied to generate cluster labels. Next, we employ TF-IDF, MMR, as well as considering sentence location information, to extract important sentences, which are used to generate surveys. Finally, we adopt manual evaluation for the generated surveys.Findings： In experiments, we choose 20 high-frequency phrases as search terms. Results show that Lingo-Word2Vec, STC-Word Net and bisecting K-means-Word2Vec have better clustering effects. In 5 points evaluation system, survey quality scores obtained by designing methods are close to 3, indicating surveys are within acceptable limits. When considering sentence location information, survey quality will be improved. Combination of Lingo, Word2Vec, TF-IDF or MMR can acquire higher survey quality.Research limitations： The manual evaluation method may have a certain subjectivity. We use a simple linear function to combine Word2Vec and Word Net that may not bring out their strengths. The generated surveys may not contain some newly created knowledge of some articles which may concentrate on sentences with no citing.Practical implications： CitationAS tool can automatically generate a comprehensive, detailed and accurate survey according to user’s search terms. It can also help researchers learn about research status in a certain field.Originality/value： Citaiton AS tool is of practicability. It merges cluster labels from semantic level to improve clustering results. The tool also considers sentence location information when calculating sentence score by TF-IDF and MMR.
