In this wide-ranging interview,world famous educator Judith Langer introduces the significance of Envisionment Building,contextualizes how to build envisionraent and critical thinking in class-room teaching.She propos...In this wide-ranging interview,world famous educator Judith Langer introduces the significance of Envisionment Building,contextualizes how to build envisionraent and critical thinking in class-room teaching.She proposes rubrics and on-going assessment in the students evaluation,which provides very insightful answers to the possible problems encountered in classroom teaching.展开更多
文摘目的研究使用En Vision多标记读板仪检测细胞内钙信号的可行性。方法分别用卡巴胆碱和凝血酶刺激LN229、SKN-MC细胞或者磷脂酶C抑制剂U73122预处理的LN229细胞,使用En Vision多标记读板仪检测细胞内钙信号强度的变化。结果卡巴胆碱诱导的LN229细胞内钙信号变化呈现浓度依赖性;卡巴胆碱和凝血酶诱导的细胞内钙信号变化趋势不同;凝血酶诱导的钙信号变化具有细胞特异性;U73122对凝血酶诱导的钙信号的抑制作用呈现剂量依赖性。结论 En Vision多标记读板仪作为细胞内钙信号检测仪器,具有快速、可重复性好和灵敏度高等优势,适用于钙信号相关的研究和小分子抑制剂的筛选等领域的工作。
文摘In this wide-ranging interview,world famous educator Judith Langer introduces the significance of Envisionment Building,contextualizes how to build envisionraent and critical thinking in class-room teaching.She proposes rubrics and on-going assessment in the students evaluation,which provides very insightful answers to the possible problems encountered in classroom teaching.
