Due to the limitations of the existing fault detection methods in the embryonic cellular array(ECA), the fault detection coverage cannot reach 100%. In order to evaluate the reliability of the ECA more accurately, emb...Due to the limitations of the existing fault detection methods in the embryonic cellular array(ECA), the fault detection coverage cannot reach 100%. In order to evaluate the reliability of the ECA more accurately, embryonic cell and its input and output(I/O) resources are considered as a whole, named functional unit(FU). The FU fault detection coverage parameter is introduced to ECA reliability analysis, and a new ECA reliability evaluation method based on the Markov status graph model is proposed.Simulation experiment results indicate that the proposed ECA reliability evaluation method can evaluate the ECA reliability more effectively and accurately. Based on the proposed reliability evaluation method, the influence of parameters change on the ECA reliability is studied, and simulation experiment results show that ECA reliability can be improved by increasing the FU fault detection coverage and reducing the FU failure rate. In addition, by increasing the scale of the ECA, the reliability increases to the maximum first, and then it will decrease continuously. ECA reliability variation rules can not only provide theoretical guidance for the ECA optimization design, but also point out the direction for further research.展开更多
Spinal cord injury repair is one of the major challenges in medicine,as it can lead to permanent loss of function of central nervous system and damage to other function of the body.Stem cell transplantation together w...Spinal cord injury repair is one of the major challenges in medicine,as it can lead to permanent loss of function of central nervous system and damage to other function of the body.Stem cell transplantation together with tissue engineering is increasingly becoming a potential choice of treatment.However,direct transplantation of stem cells without scaffolds has yielded poor clinical outcome.Here we show a strategy of using mouse embryonic stem cells(ESCs)cultured within a silk fibroin(SF)based,three-dimensional scaffold with oriented channels by a directional temperature field freezing technique and lysophilization.We find that the ESCs maintained proliferation and migrated in the scaffolds and the cells migrated fastest along the SF channels.SF scaffolds contributed to ESC differentiation into neural and glial cell like cells and expressions of the neural and glial cell markers MAP2 and GFAP were greatly elevated when retinoic acid was used as an inducing factor.Our results suggest that this approach may offer some hope in the future for spinal cord injury repair using SF scaffolds and ESCs.展开更多
RanBP1 is a binding protein of Ran that plays a pivotal role in nucleocytoplasmic transport.In this study,the localization and possible functions of RanBP1 were examined,during the early embryonic development of mice....RanBP1 is a binding protein of Ran that plays a pivotal role in nucleocytoplasmic transport.In this study,the localization and possible functions of RanBP1 were examined,during the early embryonic development of mice.Immunofluorescence results showed that RanBP1 was mainly localized in cytoplasm at mitosis interphase,and its concentration was lower in nucleus and the lowest in nucleolus.With the formation of the spindle in the early embryonic cells,RanBP1 condensed area took the shape of spindle microtubule,the concentration of RanBP1 was low in the site of chromosome.During the formation of nucleus,RanBP1 concentrated in nucleus and there were few dots of RanBP1 around the nucleolus.These dots were lost after the nucleus full growth.The results showed that RanBP1 had important roles in nucleocytoplasmic transport,spindle formation and nuclear assembly in the early embryonic development of mice.展开更多
The experiment was designed to study effects of retinoic acid and ascorbic acid on differentiation of mouse embryonic stem cells to cardiomyocytes. Embryonic bodies （EB） were developed from mESC in suspension cultur...The experiment was designed to study effects of retinoic acid and ascorbic acid on differentiation of mouse embryonic stem cells to cardiomyocytes. Embryonic bodies （EB） were developed from mESC in suspension culture, different levels of concentration of retinoic acid and ascorbic acid were used to determine the optimal conditions for EB formation. The results showed that the optimal concentrations were 10.9 mol. L-1 and 0.1 mg. mL-1 for retinoic acid and ascorbic acids, respectively. 50% of EB which was significantly （p〈0.05） different from the control group developed to cardiomyocytes. In conclusion, rctinoic acid and ascorbic acid had strong ability to promote cardiomyocyte differentiation of mouse embryonic stem cells. 10-9 mol. L-1 retinoic acid and 0.10 mg. mL-1 ascorbic acids were recommended to induce differentiation of mouse ES ceUs toward cardiomyocytes.展开更多
Embryonic stem (ES) cell biology is attracting much attention in cell biology because of their pluripotent behaviors and potential therapeutic applications. However,what maintains ES cell pluripotency and what trigger...Embryonic stem (ES) cell biology is attracting much attention in cell biology because of their pluripotent behaviors and potential therapeutic applications. However,what maintains ES cell pluripotency and what triggers ES cell展开更多
The histological observation was experimentally conducted on in vitro cultured mouse embryonic myocardium cells and myocardiumoid cell mass. The mouse embryo tissue were cultured and regular pulsatile myocardiumoid ti...The histological observation was experimentally conducted on in vitro cultured mouse embryonic myocardium cells and myocardiumoid cell mass. The mouse embryo tissue were cultured and regular pulsatile myocardiumoid tissue could be found. During in vitro culture, the myofilament bundles in the cell were gradually increasing and strongly connectted each other with embryonic age and there were loose muscle fibers initially and intercalated discs were close to each other. The lose myofilament bundles were developed in muscle fibers with age and the distance between intercalated discs was enlarged. There were myofilamentoid structure in inactive cells and filament peripherily.展开更多
Our understanding of the molecular and cellular mechanisms that control self-renewal,pluripotency and differentiation of human pluripotent stem cells(hPSCs)and our progress toward harnessing the regenerative potential...Our understanding of the molecular and cellular mechanisms that control self-renewal,pluripotency and differentiation of human pluripotent stem cells(hPSCs)and our progress toward harnessing the regenerative potential of these cells to treat human diseases are advancing at a rapid rate.Human pluripotent stem cells(hPSCs)include human embryonic stem cells(hESCs)and human induced pluripotent stem cells(hiPSCs).Their unique capacity for indefinite self-renewal(unlimited proliferation)in vitro coupled with their ability to differentiate into almost any cell type present in the adult body(pluripotency)provide a potentially展开更多
Introduction The success in lineage-specific differentiation of human embryonic and induced pluripotent stem(hES/iPS)cells raises new hopes for cell-based therapies.It is envisioned that cells differentiated from hES/...Introduction The success in lineage-specific differentiation of human embryonic and induced pluripotent stem(hES/iPS)cells raises new hopes for cell-based therapies.It is envisioned that cells differentiated from hES/iPS cells can be used to replace or repair damaged or diseased cells and tissues in body.This has not yet been possible due to the difficulty in generating biologically functional cells in vitro.While many factors may contribute to these failures,the lack of tissue niches in the current differentiation systems has been viewed in impairing the maturation of these cells.As revealed by studying mice embryo development,organ development requires strict temporal and spatial control at each stage.The stepwise hESC differentiation展开更多
AIM:Cyr61 is a secreted matrix protein belonging to the emerging CCN family.Targeted knockout of Cyr61 gene in mice results in embryonic lethality due to placental vascular insufficiency and compromised vessel integri...AIM:Cyr61 is a secreted matrix protein belonging to the emerging CCN family.Targeted knockout of Cyr61 gene in mice results in embryonic lethality due to placental vascular insufficiency and compromised vessel integrity.Cyr61 is able to stimulate neovascularization in both rat cornea and rabbit ischemic hindlimb in vivo,be rapidly induced by vascular endothelial cell growth factor and promote proliferation,migration and tube formation of vascular endothelial cells(ECs)in vitro.The present study was designed to investigate the effects of mature ECs and matrix展开更多
1 Chen A L,Liao P F,Li Q Y,Zhao Q L,Yang W K,Zhu S F,Wu F,He R F,Dong Z P,Huang P.The structural variation is associated with the embryonic lethality of a novel red egg mutant Fuyin-lre of silkworm,Bombyx mori.PLoS ON...1 Chen A L,Liao P F,Li Q Y,Zhao Q L,Yang W K,Zhu S F,Wu F,He R F,Dong Z P,Huang P.The structural variation is associated with the embryonic lethality of a novel red egg mutant Fuyin-lre of silkworm,Bombyx mori.PLoS ONE,2015,10（6）：e0128211
题目 基因结构变异导致家蚕红卵突变体Fuyin-lre的胚胎致死
摘要 家蚕有多种类型的卵色突变。虽然之前已经发现了红卵突变,但致死型红卵突变还未有相关研究报道。云南省农业科学院蚕桑蜜蜂研究所的陈安利等人对家蚕红卵突变体（Fuyin—lethal red egg，Fuyin-lre）的遗传规律进行了研究，该突变体是从家蚕种质资源Fuyin中发现的，突变性状表现为蚕卵呈红色，并且在胚胎期致死。展开更多
Regeneration from cotyledonary nodes and embryonic tips of soybean ＂Peking＂ was studied. The disinfectant ways of the mercuric chloride and chlorine gas were used and the concentrations of 6-BA and 2, 4-D were studi...Regeneration from cotyledonary nodes and embryonic tips of soybean ＂Peking＂ was studied. The disinfectant ways of the mercuric chloride and chlorine gas were used and the concentrations of 6-BA and 2, 4-D were studied in the culture medium The results showed that the sterilization effect of chlorine gas was better than that of mercuric chloride. The best concentration of 6-BA was 1.0 mg·L^-1 and the best concentration of 2, 4-D was 2.0 mg·L^-1 in the germinating medium. The number of buds of each explant was 3.56 and 2.98, respectively. The best concentrations of 6-BA and 2, 4-D were 3.0 mg·L^-1 and 3.5 mg·L^-1 in regeneration of embryonic tips. The best inducing time was 16-20 h and the mean shoots per explant was 2.69 and 2.78, respectively.展开更多
OBJECTIVE To determine the role of the basic helix-loop-helix(b HLH)transcription factor,differentiated embryonic chondrocyte gene 1(DEC1),in the apoptosis induced by 1-methyl-4-phenylpyridiniumion(MPP+)in SH-SY5Y cel...OBJECTIVE To determine the role of the basic helix-loop-helix(b HLH)transcription factor,differentiated embryonic chondrocyte gene 1(DEC1),in the apoptosis induced by 1-methyl-4-phenylpyridiniumion(MPP+)in SH-SY5Y cells.METHODS SH-SY5Y cells were treated with different concentrations of MPP+for 24or 48 h.The cell inhibition and apoptosis were measured by MTT and DAPI staining.DEC1,the apoptosis-related proteins and PI3K/Akt/GSK3β/β-catenin signaling were determined by Western blotting.The expression of DEC1was regulated by overexpression and sh RNA.RESULTS MPP+induces apoptosis along with decreasing of DEC1expression in SH-SY5Y cells.Overexpression or knockdown of DEC1 can alleviate or enhance the cell inhibition induced by MPP+.And overexpression of DEC1 can alleviate the increased cleaved caspase 3/caspase 3 but not alleviate Bax/Bcl-2 induced by MPP+.Meanwhile,MPP+represses PI3Kp110α,p-Akt/Akt,p-GSK-3β/GSK-3βandβ-catenin expression,which is accompanied by decreasing DEC1 expressions.It is confirmed that the activator or inhibitor of PI3K/Akt/GSK-3βpathway can alleviate or enhance the repression of PI3K/Akt/GSK3β/β-catenin signaling cascade induced by MPP+.Further study,we find that overexpression of DEC1 alone can increase PI3Kp110α,p-Akt/Akt,p-GSK-3β/GSK-3β,andβ-catenin expression.More importantly,overexpression of DEC1 significantly alleviates the decreased levels of PI3Kp110α,p-Akt/Akt,p-GSK-3β/GSK-3β,andβ-catenin induced by MPP+.CONCLUSION DEC1 provides neuroprotection from apoptosis induced by MPP+through PI3K/Akt pathway in SH-SY5Y cells.Promisingly,DEC1 is a candidate gene that may provide a novel therapeutic approach for the treatment of Parkinson disease.展开更多
基金supported by the National Natural Science Foundation of China(61601495,61372039)。
文摘Due to the limitations of the existing fault detection methods in the embryonic cellular array(ECA), the fault detection coverage cannot reach 100%. In order to evaluate the reliability of the ECA more accurately, embryonic cell and its input and output(I/O) resources are considered as a whole, named functional unit(FU). The FU fault detection coverage parameter is introduced to ECA reliability analysis, and a new ECA reliability evaluation method based on the Markov status graph model is proposed.Simulation experiment results indicate that the proposed ECA reliability evaluation method can evaluate the ECA reliability more effectively and accurately. Based on the proposed reliability evaluation method, the influence of parameters change on the ECA reliability is studied, and simulation experiment results show that ECA reliability can be improved by increasing the FU fault detection coverage and reducing the FU failure rate. In addition, by increasing the scale of the ECA, the reliability increases to the maximum first, and then it will decrease continuously. ECA reliability variation rules can not only provide theoretical guidance for the ECA optimization design, but also point out the direction for further research.
基金supported by funds from Huazhong University of Science and Technology,Wuhan,China
文摘Spinal cord injury repair is one of the major challenges in medicine,as it can lead to permanent loss of function of central nervous system and damage to other function of the body.Stem cell transplantation together with tissue engineering is increasingly becoming a potential choice of treatment.However,direct transplantation of stem cells without scaffolds has yielded poor clinical outcome.Here we show a strategy of using mouse embryonic stem cells(ESCs)cultured within a silk fibroin(SF)based,three-dimensional scaffold with oriented channels by a directional temperature field freezing technique and lysophilization.We find that the ESCs maintained proliferation and migrated in the scaffolds and the cells migrated fastest along the SF channels.SF scaffolds contributed to ESC differentiation into neural and glial cell like cells and expressions of the neural and glial cell markers MAP2 and GFAP were greatly elevated when retinoic acid was used as an inducing factor.Our results suggest that this approach may offer some hope in the future for spinal cord injury repair using SF scaffolds and ESCs.
基金Supported by Heilongjiang Provincial Natural Science Foundation(Face Project)(C2016021)Open Project of Key Laboratory of Feed Science,Northeast Agricultural University,Heilongjiang Province(yy-2012-10)。
文摘RanBP1 is a binding protein of Ran that plays a pivotal role in nucleocytoplasmic transport.In this study,the localization and possible functions of RanBP1 were examined,during the early embryonic development of mice.Immunofluorescence results showed that RanBP1 was mainly localized in cytoplasm at mitosis interphase,and its concentration was lower in nucleus and the lowest in nucleolus.With the formation of the spindle in the early embryonic cells,RanBP1 condensed area took the shape of spindle microtubule,the concentration of RanBP1 was low in the site of chromosome.During the formation of nucleus,RanBP1 concentrated in nucleus and there were few dots of RanBP1 around the nucleolus.These dots were lost after the nucleus full growth.The results showed that RanBP1 had important roles in nucleocytoplasmic transport,spindle formation and nuclear assembly in the early embryonic development of mice.
基金Supported by the Scientifi c Research Foundation for Doctors of Northeast Agricultural University(2012RCB27)Open Projects of Key Laboratory of Animal Genetics,Breeding and Reproduction,College of Heilongjiang Province(GXZDSYS-2012-07)
文摘The experiment was designed to study effects of retinoic acid and ascorbic acid on differentiation of mouse embryonic stem cells to cardiomyocytes. Embryonic bodies （EB） were developed from mESC in suspension culture, different levels of concentration of retinoic acid and ascorbic acid were used to determine the optimal conditions for EB formation. The results showed that the optimal concentrations were 10.9 mol. L-1 and 0.1 mg. mL-1 for retinoic acid and ascorbic acids, respectively. 50% of EB which was significantly （p〈0.05） different from the control group developed to cardiomyocytes. In conclusion, rctinoic acid and ascorbic acid had strong ability to promote cardiomyocyte differentiation of mouse embryonic stem cells. 10-9 mol. L-1 retinoic acid and 0.10 mg. mL-1 ascorbic acids were recommended to induce differentiation of mouse ES ceUs toward cardiomyocytes.
文摘Embryonic stem (ES) cell biology is attracting much attention in cell biology because of their pluripotent behaviors and potential therapeutic applications. However,what maintains ES cell pluripotency and what triggers ES cell
文摘The histological observation was experimentally conducted on in vitro cultured mouse embryonic myocardium cells and myocardiumoid cell mass. The mouse embryo tissue were cultured and regular pulsatile myocardiumoid tissue could be found. During in vitro culture, the myofilament bundles in the cell were gradually increasing and strongly connectted each other with embryonic age and there were loose muscle fibers initially and intercalated discs were close to each other. The lose myofilament bundles were developed in muscle fibers with age and the distance between intercalated discs was enlarged. There were myofilamentoid structure in inactive cells and filament peripherily.
基金supported by the National Science Foundation(CMMI 1129611, CBET 1149401,and ECCS 1231826)the American Heart Association(12SDG12180025)
文摘Our understanding of the molecular and cellular mechanisms that control self-renewal,pluripotency and differentiation of human pluripotent stem cells(hPSCs)and our progress toward harnessing the regenerative potential of these cells to treat human diseases are advancing at a rapid rate.Human pluripotent stem cells(hPSCs)include human embryonic stem cells(hESCs)and human induced pluripotent stem cells(hiPSCs).Their unique capacity for indefinite self-renewal(unlimited proliferation)in vitro coupled with their ability to differentiate into almost any cell type present in the adult body(pluripotency)provide a potentially
文摘Introduction The success in lineage-specific differentiation of human embryonic and induced pluripotent stem(hES/iPS)cells raises new hopes for cell-based therapies.It is envisioned that cells differentiated from hES/iPS cells can be used to replace or repair damaged or diseased cells and tissues in body.This has not yet been possible due to the difficulty in generating biologically functional cells in vitro.While many factors may contribute to these failures,the lack of tissue niches in the current differentiation systems has been viewed in impairing the maturation of these cells.As revealed by studying mice embryo development,organ development requires strict temporal and spatial control at each stage.The stepwise hESC differentiation
文摘AIM:Cyr61 is a secreted matrix protein belonging to the emerging CCN family.Targeted knockout of Cyr61 gene in mice results in embryonic lethality due to placental vascular insufficiency and compromised vessel integrity.Cyr61 is able to stimulate neovascularization in both rat cornea and rabbit ischemic hindlimb in vivo,be rapidly induced by vascular endothelial cell growth factor and promote proliferation,migration and tube formation of vascular endothelial cells(ECs)in vitro.The present study was designed to investigate the effects of mature ECs and matrix
文摘1 Chen A L,Liao P F,Li Q Y,Zhao Q L,Yang W K,Zhu S F,Wu F,He R F,Dong Z P,Huang P.The structural variation is associated with the embryonic lethality of a novel red egg mutant Fuyin-lre of silkworm,Bombyx mori.PLoS ONE,2015,10（6）：e0128211
题目 基因结构变异导致家蚕红卵突变体Fuyin-lre的胚胎致死
摘要 家蚕有多种类型的卵色突变。虽然之前已经发现了红卵突变,但致死型红卵突变还未有相关研究报道。云南省农业科学院蚕桑蜜蜂研究所的陈安利等人对家蚕红卵突变体（Fuyin—lethal red egg，Fuyin-lre）的遗传规律进行了研究，该突变体是从家蚕种质资源Fuyin中发现的，突变性状表现为蚕卵呈红色，并且在胚胎期致死。
基金Supported by Province Education Department Science and Technology Project (11531023)Dean Fund Project of Key Laboratory of Soybean Biologyof Ministry of Education (SB 07A04)
文摘Regeneration from cotyledonary nodes and embryonic tips of soybean ＂Peking＂ was studied. The disinfectant ways of the mercuric chloride and chlorine gas were used and the concentrations of 6-BA and 2, 4-D were studied in the culture medium The results showed that the sterilization effect of chlorine gas was better than that of mercuric chloride. The best concentration of 6-BA was 1.0 mg·L^-1 and the best concentration of 2, 4-D was 2.0 mg·L^-1 in the germinating medium. The number of buds of each explant was 3.56 and 2.98, respectively. The best concentrations of 6-BA and 2, 4-D were 3.0 mg·L^-1 and 3.5 mg·L^-1 in regeneration of embryonic tips. The best inducing time was 16-20 h and the mean shoots per explant was 2.69 and 2.78, respectively.
基金The project supported by National Natural Science Foundation of China(81573503,81373443)by the Major Project of Jiangsu Provincial Department of Education(13KJA310003)
文摘OBJECTIVE To determine the role of the basic helix-loop-helix(b HLH)transcription factor,differentiated embryonic chondrocyte gene 1(DEC1),in the apoptosis induced by 1-methyl-4-phenylpyridiniumion(MPP+)in SH-SY5Y cells.METHODS SH-SY5Y cells were treated with different concentrations of MPP+for 24or 48 h.The cell inhibition and apoptosis were measured by MTT and DAPI staining.DEC1,the apoptosis-related proteins and PI3K/Akt/GSK3β/β-catenin signaling were determined by Western blotting.The expression of DEC1was regulated by overexpression and sh RNA.RESULTS MPP+induces apoptosis along with decreasing of DEC1expression in SH-SY5Y cells.Overexpression or knockdown of DEC1 can alleviate or enhance the cell inhibition induced by MPP+.And overexpression of DEC1 can alleviate the increased cleaved caspase 3/caspase 3 but not alleviate Bax/Bcl-2 induced by MPP+.Meanwhile,MPP+represses PI3Kp110α,p-Akt/Akt,p-GSK-3β/GSK-3βandβ-catenin expression,which is accompanied by decreasing DEC1 expressions.It is confirmed that the activator or inhibitor of PI3K/Akt/GSK-3βpathway can alleviate or enhance the repression of PI3K/Akt/GSK3β/β-catenin signaling cascade induced by MPP+.Further study,we find that overexpression of DEC1 alone can increase PI3Kp110α,p-Akt/Akt,p-GSK-3β/GSK-3β,andβ-catenin expression.More importantly,overexpression of DEC1 significantly alleviates the decreased levels of PI3Kp110α,p-Akt/Akt,p-GSK-3β/GSK-3β,andβ-catenin induced by MPP+.CONCLUSION DEC1 provides neuroprotection from apoptosis induced by MPP+through PI3K/Akt pathway in SH-SY5Y cells.Promisingly,DEC1 is a candidate gene that may provide a novel therapeutic approach for the treatment of Parkinson disease.
