An online dynamic method based on electrical conductivity probe, tensiometer and datataker was presented to measure saturation-capillary pressure （S-p） relation in water-light nonaqueous phase liquid （LNAPL） two-p...An online dynamic method based on electrical conductivity probe, tensiometer and datataker was presented to measure saturation-capillary pressure （S-p） relation in water-light nonaqueous phase liquid （LNAPL） two-phase sandy medium under water level fluctuation. Three-electrode electrical conductivity probe （ECP） was used to measure water saturation. Hydrophobic tensiometer was obtained by spraying waterproof material to the ceramic cup of commercially available hydrophilic tensiometer. A couple of hydrophilic tensiometer and hydrophobic tensiometer were used to measure pore water pressure and pore LNAPL pressure of the sandy medium, respectively. All the signals from ECP and tensiometer were collected by a data taker connected with a computer. The results show that this method can finish the measurement of S-R relation of a complete drainage or imbibition process in less than 60 min. It is much more timesaving compared with 10-40 d of traditional methods. Two cycles of water level fluctuation were produced, and four saturation-capillary pressure relations including two stable residual LNAPL saturations of the sandy medium were obtained during in 350 h. The results show that this method has a good durable performance and feasibility in the porous medium with complicated multiphase flow. Although further studies are needed on the signal stability and accuracy drift of the ECP, this online dynamic method can be used successfully in the rapid characterization of a LNAPL migration in porous media.展开更多
The Jinchuan Ni-Cu sulfide deposits,NW China,are hosted in small ultramafic intrusions that were emplaced into Paleoproterozoic metamorphic rocks. The ultramafic intrusions were previously thought to be the segments o...The Jinchuan Ni-Cu sulfide deposits,NW China,are hosted in small ultramafic intrusions that were emplaced into Paleoproterozoic metamorphic rocks. The ultramafic intrusions were previously thought to be the segments of a single elongate intrusion that was dismembered by late faults into eastern and western portions,each of which have distinct stratigraphic sequences.展开更多
As the air combat environment becomes more complicated and changeable, accurate threat assessment of air target has a significant impact on air defense operations. This paper proposes an improved generalized intuition...As the air combat environment becomes more complicated and changeable, accurate threat assessment of air target has a significant impact on air defense operations. This paper proposes an improved generalized intuitionistic fuzzy soft set (GIFSS) method for dynamic assessment of air target threat. Firstly, the threat assessment index is reasonably determined by analyzing the typical characteristics of air targets. Secondly, after the GIFSS at different time is obtained, the index weight is determined by the intuitionistic fuzzy set entropy and the relative entropy theory. Then, the inverse Poisson distribution method is used to determine the weight of time series, and then the time-weighted GIFSS is obtained. Finally, threat assessment of five air targets is carried out by using the improved GIFSS (I-GIFSS) and comparison methods. The validity and superiority of the proposed method are verified by calculation and comparison.展开更多
Accurate 3-D fracture network model for rock mass in dam foundation is of vital importance for stability,grouting and seepage analysis of dam foundation.With the aim of reducing deviation between fracture network mode...Accurate 3-D fracture network model for rock mass in dam foundation is of vital importance for stability,grouting and seepage analysis of dam foundation.With the aim of reducing deviation between fracture network model and measured data,a 3-D fracture network dynamic modeling method based on error analysis was proposed.Firstly,errors of four fracture volume density estimation methods(proposed by ODA,KULATILAKE,MAULDON,and SONG)and that of four fracture size estimation methods(proposed by EINSTEIN,SONG and TONON)were respectively compared,and the optimal methods were determined.Additionally,error index representing the deviation between fracture network model and measured data was established with integrated use of fractal dimension and relative absolute error(RAE).On this basis,the downhill simplex method was used to build the dynamic modeling method,which takes the minimum of error index as objective function and dynamically adjusts the fracture density and size parameters to correct the error index.Finally,the 3-D fracture network model could be obtained which meets the requirements.The proposed method was applied for 3-D fractures simulation in Miao Wei hydropower project in China for feasibility verification and the error index reduced from 2.618 to 0.337.展开更多
A finite element model for the supercavitating underwater vehicle was developed by employing 16-node shell elements of relative degrees of freedom.The nonlinear structural dynamic response was performed by introducing...A finite element model for the supercavitating underwater vehicle was developed by employing 16-node shell elements of relative degrees of freedom.The nonlinear structural dynamic response was performed by introducing the updated Lagrangian formulation.The numerical results indicate that there exists a critical thickness for the supercavitating plain shell for the considered velocity of the vehicle.The structure fails more easily because of instability with the thickness less than the critical value,while the structure maintains dynamic stability with the thickness greater than the critical value.As the velocity of the vehicle increases,the critical thickness for the plain shell increases accordingly.For the considered structural configuration,the critical thicknesses of plain shells are 5 and 7 mm for the velocities of 300 and 400 m/s,respectively.The structural stability is enhanced by using the stiffened configuration.With the shell configuration of nine ring stiffeners,the maximal displacement and von Mises stress of the supercavitating structure decrease by 25% and 17% for the velocity of 300 m/s,respectively.Compared with ring stiffeners,longitudinal stiffeners are more significant to improve structural dynamic performance and decrease the critical value of thickness of the shell for the supercavitating vehicle.展开更多
Integrin activation,the transition from a low to a high affinity state,regulates the numerous cellular responses consequent to integrin engagement by extracellular matrix proteins.Kindlin proteins,play crucial roles i...Integrin activation,the transition from a low to a high affinity state,regulates the numerous cellular responses consequent to integrin engagement by extracellular matrix proteins.Kindlin proteins,play crucial roles in the integrin-signaling pathway by directly interacting with and activating integrins,which mediate the cell-extracellular matrix adhesion and signaling.As a widely distributed PTB domain protein and a major member of the kindlin family,kindlin2 interacts withβ3-tail,bridges talin-activated integrins to promote integrin aggregation,and enhances talin-induced integrin activation.Thus,kindlin2 is identified as a coactivator of integrins.Unlike talins,kindlin2 cannot directly alter the conformation of the integrin transmembrane helix and fail to activate integrin alone.Nevertheless,although it is widely accepted that kindlins and talins synergistically promote integrin activation,the underlying mechanism is unclear.Thus,the study of the force dissociation of the kindlin2/β3-tail complex and the conformation stabilization under different mechanical micro-environments should be of great significance for the further understanding of the structural basis of its synergistically activation of integrin.To reveal the molecular dynamics mechanism of interaction between kindlin2 andβ3-tail,we perform molecular dynamics(MD)simulations for this complex with different computing strategies interaction.In MD simulations,the available crystal structures of Kindlin-2/β3-tail complex(Protein Data Bank code 5XQ1)was downloaded from the PDB database.Two software packages,VMD for visualization and modeling and NAMD 2.13 for energy minimizations and MD simulations,were used here.The steadystate conformation of the complex was obtained from the equilibrium simulation.The dissociation event was observed by the constant velocity simulation,and the mechanical stability of the complex was observed by the constant force simulation.Our results showed that,during the equilibrium of the kindlin2-F3/β34ail complex,the residue MET612,LYS613 and TRP615 on the F3 domain of kindlin2 contributed to hydrogen-bonding with the corresponding residues onβ3 integrin.These bonds exhibit moderate or strong stability through steered molecular dynamics(SMD)simulation.During the constant velocity simulation,the complex exhibits a variety of unfolding pathways against tension applications,which are mainly distinguished by the disruption of hydrogen-bonds between the F3 domain a1/a2 helixes andβ1/β2 sheets.During the constant force simulation,the different phases of the composite force dissociation have different dissociation probabilities,which shows the biphasic force-dependent characteristics.And,the key residues in the pulling were recognized according not only to the number of interacting residue pairs,but also to their bond strength.Using molecular dynamics simulation,we showed the steady state of the kindlin2-F3/β3-tail complex under different tensile forces,and observe the dynamic process of molecular interaction.A possible underlying biophysical mechanism is that,the dissociation of Kindlin2-F3/β3-tail complex is biphasic force-dependent,and the conformations under different stretching states have different binding affinities.This study not only provides insights into the structural basis and mechanical regulation mechanisms of the kindlin/integrin interaction,in understanding in kindlin/integrin-related signaling in different cellular biological processes,but also provides new ideas for novel drug design and the treatment of related diseases.展开更多
According to the existing problems of liquefaction models of saturated sand that were put forward under dynamic action,on the basis of Handin-Drnevich model,a new calculating model of the dynamic constitutive relation...According to the existing problems of liquefaction models of saturated sand that were put forward under dynamic action,on the basis of Handin-Drnevich model,a new calculating model of the dynamic constitutive relation of saturated sand was put forward.The model was based on the basic hypothesis of instantaneous limit balance according to the basic principle that the stress estate is the destroyed condition was not overstepped.The calculated method of increment nonlinear was referenced and combined with the excellence of the model of distributed particles.The process of vibrating liquefaction of saturated sand was divided into some areas.And the phenomena of shearing dilatation and unloading shrink of saturated sand were considered.On above basic a new calculating constitutive relation model was proposed.There are a few parameters in the model.The physical means of the parameters are very evident and quantized.They could be obtained from the dynamic triaxial test in door.The model was contrasted and validated with the results of the dynamic triaxial test in door.The comparison of the results of the dynamic triaxial test in door and the calculating results of the model indicates that all sorts of phenomenon appearing in the process of liquefaction of saturated sand could be more perfectly reflected by the model.Especially at the initial stage of development of pore water pressure and strain of saturated sand,the results of the dynamic triaxial test in door are consistent with the calculated results of the model very much.But there is some difference between the results at the anaphase of development of pore water pressure and strain.On the path of stress,the calculating and experimenting ultimate state surfaces are almost identical.展开更多
基金Project(8151027501000008) supported by Guangdong Natural Science Foundation, ChinaProject(2007490511) supported by the Open Foundation of State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering, Hohai University, ChinaProject (2006K0006) supported by the Open Foundation of Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, China
文摘An online dynamic method based on electrical conductivity probe, tensiometer and datataker was presented to measure saturation-capillary pressure （S-p） relation in water-light nonaqueous phase liquid （LNAPL） two-phase sandy medium under water level fluctuation. Three-electrode electrical conductivity probe （ECP） was used to measure water saturation. Hydrophobic tensiometer was obtained by spraying waterproof material to the ceramic cup of commercially available hydrophilic tensiometer. A couple of hydrophilic tensiometer and hydrophobic tensiometer were used to measure pore water pressure and pore LNAPL pressure of the sandy medium, respectively. All the signals from ECP and tensiometer were collected by a data taker connected with a computer. The results show that this method can finish the measurement of S-R relation of a complete drainage or imbibition process in less than 60 min. It is much more timesaving compared with 10-40 d of traditional methods. Two cycles of water level fluctuation were produced, and four saturation-capillary pressure relations including two stable residual LNAPL saturations of the sandy medium were obtained during in 350 h. The results show that this method has a good durable performance and feasibility in the porous medium with complicated multiphase flow. Although further studies are needed on the signal stability and accuracy drift of the ECP, this online dynamic method can be used successfully in the rapid characterization of a LNAPL migration in porous media.
文摘The Jinchuan Ni-Cu sulfide deposits,NW China,are hosted in small ultramafic intrusions that were emplaced into Paleoproterozoic metamorphic rocks. The ultramafic intrusions were previously thought to be the segments of a single elongate intrusion that was dismembered by late faults into eastern and western portions,each of which have distinct stratigraphic sequences.
基金supported by the National Natural Science Foundation of China(51779263)
文摘As the air combat environment becomes more complicated and changeable, accurate threat assessment of air target has a significant impact on air defense operations. This paper proposes an improved generalized intuitionistic fuzzy soft set (GIFSS) method for dynamic assessment of air target threat. Firstly, the threat assessment index is reasonably determined by analyzing the typical characteristics of air targets. Secondly, after the GIFSS at different time is obtained, the index weight is determined by the intuitionistic fuzzy set entropy and the relative entropy theory. Then, the inverse Poisson distribution method is used to determine the weight of time series, and then the time-weighted GIFSS is obtained. Finally, threat assessment of five air targets is carried out by using the improved GIFSS (I-GIFSS) and comparison methods. The validity and superiority of the proposed method are verified by calculation and comparison.
基金Project(51321065)supported by the Innovative Research Groups of the National Natural Science Foundation of ChinaProject(2013CB035904)supported by the National Basic Research Program of China(973 Program)Project(51439005)supported by the National Natural Science Foundation of China
文摘Accurate 3-D fracture network model for rock mass in dam foundation is of vital importance for stability,grouting and seepage analysis of dam foundation.With the aim of reducing deviation between fracture network model and measured data,a 3-D fracture network dynamic modeling method based on error analysis was proposed.Firstly,errors of four fracture volume density estimation methods(proposed by ODA,KULATILAKE,MAULDON,and SONG)and that of four fracture size estimation methods(proposed by EINSTEIN,SONG and TONON)were respectively compared,and the optimal methods were determined.Additionally,error index representing the deviation between fracture network model and measured data was established with integrated use of fractal dimension and relative absolute error(RAE).On this basis,the downhill simplex method was used to build the dynamic modeling method,which takes the minimum of error index as objective function and dynamically adjusts the fracture density and size parameters to correct the error index.Finally,the 3-D fracture network model could be obtained which meets the requirements.The proposed method was applied for 3-D fractures simulation in Miao Wei hydropower project in China for feasibility verification and the error index reduced from 2.618 to 0.337.
文摘A finite element model for the supercavitating underwater vehicle was developed by employing 16-node shell elements of relative degrees of freedom.The nonlinear structural dynamic response was performed by introducing the updated Lagrangian formulation.The numerical results indicate that there exists a critical thickness for the supercavitating plain shell for the considered velocity of the vehicle.The structure fails more easily because of instability with the thickness less than the critical value,while the structure maintains dynamic stability with the thickness greater than the critical value.As the velocity of the vehicle increases,the critical thickness for the plain shell increases accordingly.For the considered structural configuration,the critical thicknesses of plain shells are 5 and 7 mm for the velocities of 300 and 400 m/s,respectively.The structural stability is enhanced by using the stiffened configuration.With the shell configuration of nine ring stiffeners,the maximal displacement and von Mises stress of the supercavitating structure decrease by 25% and 17% for the velocity of 300 m/s,respectively.Compared with ring stiffeners,longitudinal stiffeners are more significant to improve structural dynamic performance and decrease the critical value of thickness of the shell for the supercavitating vehicle.
基金supported by the National Natural Science Foundation of China ( 116272109, 11432006)
文摘Integrin activation,the transition from a low to a high affinity state,regulates the numerous cellular responses consequent to integrin engagement by extracellular matrix proteins.Kindlin proteins,play crucial roles in the integrin-signaling pathway by directly interacting with and activating integrins,which mediate the cell-extracellular matrix adhesion and signaling.As a widely distributed PTB domain protein and a major member of the kindlin family,kindlin2 interacts withβ3-tail,bridges talin-activated integrins to promote integrin aggregation,and enhances talin-induced integrin activation.Thus,kindlin2 is identified as a coactivator of integrins.Unlike talins,kindlin2 cannot directly alter the conformation of the integrin transmembrane helix and fail to activate integrin alone.Nevertheless,although it is widely accepted that kindlins and talins synergistically promote integrin activation,the underlying mechanism is unclear.Thus,the study of the force dissociation of the kindlin2/β3-tail complex and the conformation stabilization under different mechanical micro-environments should be of great significance for the further understanding of the structural basis of its synergistically activation of integrin.To reveal the molecular dynamics mechanism of interaction between kindlin2 andβ3-tail,we perform molecular dynamics(MD)simulations for this complex with different computing strategies interaction.In MD simulations,the available crystal structures of Kindlin-2/β3-tail complex(Protein Data Bank code 5XQ1)was downloaded from the PDB database.Two software packages,VMD for visualization and modeling and NAMD 2.13 for energy minimizations and MD simulations,were used here.The steadystate conformation of the complex was obtained from the equilibrium simulation.The dissociation event was observed by the constant velocity simulation,and the mechanical stability of the complex was observed by the constant force simulation.Our results showed that,during the equilibrium of the kindlin2-F3/β34ail complex,the residue MET612,LYS613 and TRP615 on the F3 domain of kindlin2 contributed to hydrogen-bonding with the corresponding residues onβ3 integrin.These bonds exhibit moderate or strong stability through steered molecular dynamics(SMD)simulation.During the constant velocity simulation,the complex exhibits a variety of unfolding pathways against tension applications,which are mainly distinguished by the disruption of hydrogen-bonds between the F3 domain a1/a2 helixes andβ1/β2 sheets.During the constant force simulation,the different phases of the composite force dissociation have different dissociation probabilities,which shows the biphasic force-dependent characteristics.And,the key residues in the pulling were recognized according not only to the number of interacting residue pairs,but also to their bond strength.Using molecular dynamics simulation,we showed the steady state of the kindlin2-F3/β3-tail complex under different tensile forces,and observe the dynamic process of molecular interaction.A possible underlying biophysical mechanism is that,the dissociation of Kindlin2-F3/β3-tail complex is biphasic force-dependent,and the conformations under different stretching states have different binding affinities.This study not only provides insights into the structural basis and mechanical regulation mechanisms of the kindlin/integrin interaction,in understanding in kindlin/integrin-related signaling in different cellular biological processes,but also provides new ideas for novel drug design and the treatment of related diseases.
基金Project(59979001) supported by the National Natural Science Foundation of China
文摘According to the existing problems of liquefaction models of saturated sand that were put forward under dynamic action,on the basis of Handin-Drnevich model,a new calculating model of the dynamic constitutive relation of saturated sand was put forward.The model was based on the basic hypothesis of instantaneous limit balance according to the basic principle that the stress estate is the destroyed condition was not overstepped.The calculated method of increment nonlinear was referenced and combined with the excellence of the model of distributed particles.The process of vibrating liquefaction of saturated sand was divided into some areas.And the phenomena of shearing dilatation and unloading shrink of saturated sand were considered.On above basic a new calculating constitutive relation model was proposed.There are a few parameters in the model.The physical means of the parameters are very evident and quantized.They could be obtained from the dynamic triaxial test in door.The model was contrasted and validated with the results of the dynamic triaxial test in door.The comparison of the results of the dynamic triaxial test in door and the calculating results of the model indicates that all sorts of phenomenon appearing in the process of liquefaction of saturated sand could be more perfectly reflected by the model.Especially at the initial stage of development of pore water pressure and strain of saturated sand,the results of the dynamic triaxial test in door are consistent with the calculated results of the model very much.But there is some difference between the results at the anaphase of development of pore water pressure and strain.On the path of stress,the calculating and experimenting ultimate state surfaces are almost identical.
