1997年,第10号染色体缺失的磷酸酶与张力蛋白同源物基因（phosphatase and tensin homolog deleted on chromosome 10,PTEN）由国外3个独立的研究小组先后克隆命名,这是目前发现的第一个具有磷酸酶活性的抑癌基因,其编码的蛋白在细胞内...1997年,第10号染色体缺失的磷酸酶与张力蛋白同源物基因（phosphatase and tensin homolog deleted on chromosome 10,PTEN）由国外3个独立的研究小组先后克隆命名,这是目前发现的第一个具有磷酸酶活性的抑癌基因,其编码的蛋白在细胞内具有脂质磷酸酶和蛋白磷酸酶的双重活性。PTEN具有广泛的生物学功能,通过细胞内多个信号通路来调控细胞的生长、分化、迁移、凋亡等过程。展开更多
Herpes simplex virus type 1 (HSV-1) naturallyestablishes latency in neurons of the nervous systemwith the concommitant expression of the latencyassociated transcripts (LATS) and the loss in
Chain length of closed circle DNA is equal. The same closed circle DNA＇s position corresponds to different recognition sequence, and the same recognition sequence corresponds to different foreign DNA segment, so clos...Chain length of closed circle DNA is equal. The same closed circle DNA＇s position corresponds to different recognition sequence, and the same recognition sequence corresponds to different foreign DNA segment, so closed circle DNA computing model is generalized. For change positive-weighted Hamilton circuit problem, closed circle DNA algorithm is put forward. First, three groups of DNA encoding are encoded for all arcs, and deck groups are designed for all vertices. All possible solutions are composed. Then, the feasible solutions are filtered out by using group detect experiment, and the optimization solutions are obtained by using group insert experiment and electrophoresis experiment. Finally, all optimization solutions are found by using detect experiment. Complexity of algorithm is concluded and validity of DNA algorithm is explained by an example. Three dominances of the closed circle DNA algorithm are analyzed, and characteristics and dominances of group delete experiment are discussed.展开更多
The CDKN2 (MTS1/P16<sup>INK4A</sup>) is believed as atumor suppressor gene. It maps in the human’schromosome gp21. It encodes a p16<sup>INK4A</sup> protein thatis an inhibitor of cyclin-depend...The CDKN2 (MTS1/P16<sup>INK4A</sup>) is believed as atumor suppressor gene. It maps in the human’schromosome gp21. It encodes a p16<sup>INK4A</sup> protein thatis an inhibitor of cyclin-dependent kinase 4. CDKN2gene’s homozygous deletion is common in many tumorderived cell lines. Purpose: We examine展开更多
During the past ten years. our molecular studies haddemonstrated that myelodysplasia of MDS bone marrowwas associated with the rearrangement/amplification ofc-erbB and deletion/ inactivation of c-erbA and identifiedth...During the past ten years. our molecular studies haddemonstrated that myelodysplasia of MDS bone marrowwas associated with the rearrangement/amplification ofc-erbB and deletion/ inactivation of c-erbA and identifiedthe sites of the rearrangement by v-erbB PCR genediagnosis of MDS and confirmed their etiogenicsignificance by the follow-up of MDS cases. These resultsprovided 2 target sequences of c-erbB.The展开更多
Human DNA polymerase η,a low-fidelity DNA polymerase,plays an important role in genesis of non-targeted mutations in cells in response to the mutagen and carcinogen N-methyl-N’-nitro-N-nitrosoguanidine(MNNG).In this...Human DNA polymerase η,a low-fidelity DNA polymerase,plays an important role in genesis of non-targeted mutations in cells in response to the mutagen and carcinogen N-methyl-N’-nitro-N-nitrosoguanidine(MNNG).In this study,we showed that the expression of Pol η was up-regulated in human amnion FL cells stimulated by MNNG,and the putative promoter of POLH gene was activated in response to MNNG treatment.Reporter gene assays with deletion constructs of the POLH promoter展开更多
When detecting deletions in complex human genomes,split-read approaches using short reads generated with next-generation sequencing still face the challenge that either false discovery rate is high,or sensitivity is l...When detecting deletions in complex human genomes,split-read approaches using short reads generated with next-generation sequencing still face the challenge that either false discovery rate is high,or sensitivity is low.To address the problem,an integrated strategy is proposed.It organically combines the fundamental theories of the three mainstream methods(read-pair approaches,split-read technologies and read-depth analysis) with modern machine learning algorithms,using the recipe of feature extraction as a bridge.Compared with the state-of-art split-read methods for deletion detection in both low and high sequence coverage,the machine-learning-aided strategy shows great ability in intelligently balancing sensitivity and false discovery rate and getting a both more sensitive and more precise call set at single-base-pair resolution.Thus,users do not need to rely on former experience to make an unnecessary trade-off beforehand and adjust parameters over and over again any more.It should be noted that modern machine learning models can play an important role in the field of structural variation prediction.展开更多
文摘1997年,第10号染色体缺失的磷酸酶与张力蛋白同源物基因（phosphatase and tensin homolog deleted on chromosome 10,PTEN）由国外3个独立的研究小组先后克隆命名,这是目前发现的第一个具有磷酸酶活性的抑癌基因,其编码的蛋白在细胞内具有脂质磷酸酶和蛋白磷酸酶的双重活性。PTEN具有广泛的生物学功能,通过细胞内多个信号通路来调控细胞的生长、分化、迁移、凋亡等过程。
文摘Herpes simplex virus type 1 (HSV-1) naturallyestablishes latency in neurons of the nervous systemwith the concommitant expression of the latencyassociated transcripts (LATS) and the loss in
基金supported by the National Natural Science Foundation of China(60574041)the Natural ScienceFoundation of Hubei Province(2007ABA407).
文摘Chain length of closed circle DNA is equal. The same closed circle DNA＇s position corresponds to different recognition sequence, and the same recognition sequence corresponds to different foreign DNA segment, so closed circle DNA computing model is generalized. For change positive-weighted Hamilton circuit problem, closed circle DNA algorithm is put forward. First, three groups of DNA encoding are encoded for all arcs, and deck groups are designed for all vertices. All possible solutions are composed. Then, the feasible solutions are filtered out by using group detect experiment, and the optimization solutions are obtained by using group insert experiment and electrophoresis experiment. Finally, all optimization solutions are found by using detect experiment. Complexity of algorithm is concluded and validity of DNA algorithm is explained by an example. Three dominances of the closed circle DNA algorithm are analyzed, and characteristics and dominances of group delete experiment are discussed.
文摘The CDKN2 (MTS1/P16<sup>INK4A</sup>) is believed as atumor suppressor gene. It maps in the human’schromosome gp21. It encodes a p16<sup>INK4A</sup> protein thatis an inhibitor of cyclin-dependent kinase 4. CDKN2gene’s homozygous deletion is common in many tumorderived cell lines. Purpose: We examine
文摘During the past ten years. our molecular studies haddemonstrated that myelodysplasia of MDS bone marrowwas associated with the rearrangement/amplification ofc-erbB and deletion/ inactivation of c-erbA and identifiedthe sites of the rearrangement by v-erbB PCR genediagnosis of MDS and confirmed their etiogenicsignificance by the follow-up of MDS cases. These resultsprovided 2 target sequences of c-erbB.The
基金Supported by the National Natural Science Foundation of China(No.30770831No.J0730856)Zhejiang Provincial Natural Science Foundation for Excellent Research Groups(No.R207153)
文摘Human DNA polymerase η,a low-fidelity DNA polymerase,plays an important role in genesis of non-targeted mutations in cells in response to the mutagen and carcinogen N-methyl-N’-nitro-N-nitrosoguanidine(MNNG).In this study,we showed that the expression of Pol η was up-regulated in human amnion FL cells stimulated by MNNG,and the putative promoter of POLH gene was activated in response to MNNG treatment.Reporter gene assays with deletion constructs of the POLH promoter
基金Project(61472026)supported by the National Natural Science Foundation of ChinaProject(2014J410081)supported by Guangzhou Scientific Research Program,China
文摘When detecting deletions in complex human genomes,split-read approaches using short reads generated with next-generation sequencing still face the challenge that either false discovery rate is high,or sensitivity is low.To address the problem,an integrated strategy is proposed.It organically combines the fundamental theories of the three mainstream methods(read-pair approaches,split-read technologies and read-depth analysis) with modern machine learning algorithms,using the recipe of feature extraction as a bridge.Compared with the state-of-art split-read methods for deletion detection in both low and high sequence coverage,the machine-learning-aided strategy shows great ability in intelligently balancing sensitivity and false discovery rate and getting a both more sensitive and more precise call set at single-base-pair resolution.Thus,users do not need to rely on former experience to make an unnecessary trade-off beforehand and adjust parameters over and over again any more.It should be noted that modern machine learning models can play an important role in the field of structural variation prediction.
